Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 1,844,787 G→A R199Q (CGG→CAG)  RPPX_08230 → monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099741,844,7870GA99.4% 1327.4 / NA 353R199Q (CGG→CAG) RPPX_08230monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (162/186);  total (166/187)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

CTCACCCTCAAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCGGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATCCCCAACATTCA  >  CP009974/1844639‑1844934
                                                                                                                                                    |                                                                                                                                                   
ctcaccctcaAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGATCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCagg                                                                                                                                                   >  2:3904797/1‑151 (MQ=255)
   accctcaAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGaa                                                                                                                                                <  2:1608810/151‑1 (MQ=255)
     cctcaAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGc                                                                                                                                              >  1:6095141/1‑151 (MQ=255)
     cctcaAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGc                                                                                                                                              <  1:2672820/151‑1 (MQ=255)
       tcaAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGccc                                                                                                                                            <  1:3540752/151‑1 (MQ=255)
       tcaAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGccc                                                                                                                                            >  2:4883335/1‑151 (MQ=255)
          aGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCggg                                                                                                                                         >  1:5812689/1‑151 (MQ=255)
            gCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGt                                                                                                                                       <  1:3351567/151‑1 (MQ=255)
              ccGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTg                                                                                                                                     <  1:910851/151‑1 (MQ=255)
                gAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTgcg                                                                                                                                   >  1:4671831/1‑151 (MQ=255)
                gAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTgcg                                                                                                                                   >  1:1929577/1‑151 (MQ=255)
                gAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTgcg                                                                                                                                   >  1:132818/1‑151 (MQ=255)
                gAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTgcg                                                                                                                                   >  1:2860826/1‑151 (MQ=255)
                 aaTTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTgcgc                                                                                                                                  >  1:4854607/1‑151 (MQ=255)
                  aTTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCa                                                                                                                                 >  2:4086916/1‑151 (MQ=255)
                         gcgcCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGa                                                                                                                          >  2:1005080/1‑151 (MQ=255)
                          cgcCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCCCCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGAt                                                                                                                         <  1:4975317/151‑1 (MQ=255)
                            ccGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTACAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAGGCCCGGGGTTGCGCATGGTCGATGa                                                                                                                       >  2:3609684/1‑151 (MQ=255)
                            ccGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGa                                                                                                                       <  1:4365542/151‑1 (MQ=255)
                              ggCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACt                                                                                                                     >  2:2332451/1‑151 (MQ=255)
                               gCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTg                                                                                                                    <  1:3590410/151‑1 (MQ=255)
                                  aCAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTc                                                                                                                 >  1:5710421/1‑151 (MQ=255)
                                   cAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTcc                                                                                                                >  1:1834508/1‑151 (MQ=255)
                                   cAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTGCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTcc                                                                                                                <  1:225298/151‑1 (MQ=255)
                                    aGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGATGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCg                                                                                                               <  2:5245848/151‑1 (MQ=255)
                                      cTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCggg                                                                                                             <  1:598152/151‑1 (MQ=255)
                                      cTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCggg                                                                                                             <  1:113252/151‑1 (MQ=255)
                                            cATCAGTGGCGAGAAGTTCTACGCCACCGGTGCCGTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGGCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGc                                                                                                       <  1:6425002/151‑1 (MQ=255)
                                              tAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCa                                                                                                     >  2:3813410/1‑151 (MQ=255)
                                               aaGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAg                                                                                                    >  1:3967827/1‑151 (MQ=255)
                                               aaGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAg                                                                                                    <  1:3283517/151‑1 (MQ=255)
                                                aGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAgc                                                                                                   >  1:4057155/1‑151 (MQ=255)
                                                aGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAgc                                                                                                   <  2:5531357/151‑1 (MQ=255)
                                                 gTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAgcg                                                                                                  <  2:56127/151‑1 (MQ=255)
                                                  tGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAgcgc                                                                                                 >  2:1540673/1‑151 (MQ=255)
                                                    gCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGcac                                                                                               >  2:2123102/1‑151 (MQ=255)
                                                     cGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGcacc                                                                                              >  1:779258/1‑151 (MQ=255)
                                                        gaAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCaccacc                                                                                           >  1:1889938/1‑151 (MQ=255)
                                                         aaGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCg                                                                                          <  1:4281302/151‑1 (MQ=255)
                                                            ttCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCa                                                                                       >  2:4180243/1‑151 (MQ=255)
                                                            ttCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAAGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCa                                                                                       >  2:1174795/1‑151 (MQ=255)
                                                              cTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGc                                                                                     >  2:1106377/1‑151 (MQ=255)
                                                               tACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCg                                                                                    <  2:2792735/151‑1 (MQ=255)
                                                                aCTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCgg                                                                                   >  2:3033033/1‑151 (MQ=255)
                                                                aCTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCgg                                                                                   >  2:1036507/1‑151 (MQ=255)
                                                                  tCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCCGCGGTa                                                                                 >  2:2198047/1‑151 (MQ=255)
                                                                  tCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTa                                                                                 >  2:6412751/1‑151 (MQ=255)
                                                                  tCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTa                                                                                 <  1:6047557/151‑1 (MQ=255)
                                                                    cACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTAcc                                                                               <  2:3430375/151‑1 (MQ=255)
                                                                     aCCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGTCGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCTGGGAAGCCCGGGGTTGCGCATTGTGGATTTCTGGTTCGGGGTCGGCAGGCGCACCACCGCCATCGGTAccc                                                                              >  2:3746899/1‑150 (MQ=255)
                                                                      ccGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGt                                                                             <  1:5837267/151‑1 (MQ=255)
                                                                       cGGTGCCTTGTTCGCCCATTGGGTGGCTGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGAGGACTGGGCCGGGTTCGGCCGGCGCACCACCGCCAGCGGTTCCGtg                                                                            >  2:2690791/1‑151 (MQ=255)
                                                                         gTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGtgct                                                                          >  1:5100779/1‑151 (MQ=255)
                                                                            ccTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTgctgct                                                                       <  1:1208600/151‑1 (MQ=255)
                                                                            ccTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTgctgct                                                                       >  1:254915/1‑151 (MQ=255)
                                                                              ttGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTgg                                                                     <  2:2098346/151‑1 (MQ=255)
                                                                              ttGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTgg                                                                     >  2:3546364/1‑151 (MQ=255)
                                                                                gTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGAc                                                                   <  1:1491798/151‑1 (MQ=255)
                                                                                   cGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAg                                                                >  2:1451737/1‑151 (MQ=255)
                                                                                   cGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAg                                                                <  2:616277/151‑1 (MQ=255)
                                                                                   cGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGAGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGCACCAg                                                                >  2:1982018/1‑151 (MQ=255)
                                                                                      ccATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTg                                                             <  2:1808321/151‑1 (MQ=255)
                                                                                           gggTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGt                                                        >  2:534848/1‑151 (MQ=255)
                                                                                              tGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGa                                                     >  1:4546514/1‑151 (MQ=255)
                                                                                              tGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGa                                                     >  2:3193793/1‑151 (MQ=255)
                                                                                                  ggTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGAcgcg                                                 <  2:4006627/151‑1 (MQ=255)
                                                                                                   gTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCgg                                                >  1:245405/1‑151 (MQ=255)
                                                                                                   gTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCgg                                                <  2:1523957/151‑1 (MQ=255)
                                                                                                       aaGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACt                                            >  2:1740078/1‑151 (MQ=255)
                                                                                                       aaGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACt                                            <  2:1369532/151‑1 (MQ=255)
                                                                                                        aGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTg                                           >  1:5929148/1‑151 (MQ=255)
                                                                                                              cgacgacgaAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATc                                     >  2:873609/1‑151 (MQ=255)
                                                                                                               gacgacgaAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCCTTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCg                                    <  2:5464864/151‑1 (MQ=255)
                                                                                                               gacgacgaAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCg                                    >  1:208793/1‑151 (MQ=255)
                                                                                                                  gacgaAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACa                                 <  1:1028752/151‑1 (MQ=255)
                                                                                                                  gacgaAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACa                                 >  2:1890767/1‑151 (MQ=255)
                                                                                                                  gacgaAGGCCGCCAGCGCCTGCCATTCGTGCGCCAGGGGAGCCCGGGCTTGCCCGTTGTCGATGACGCGACCGGGTTCGGCCACCGCACCACCGCCCGCGGTACCGCCCTGCTGGACCAGCCGCCGGTTGACGCAGAACCGGTGATCGAcc                                 >  2:2583621/1‑150 (MQ=255)
                                                                                                                     gaAGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACt                              <  1:6268290/151‑1 (MQ=255)
                                                                                                                      aaGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCGTTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTg                             <  2:6260329/151‑1 (MQ=255)
                                                                                                                      aaGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTg                             <  2:679467/151‑1 (MQ=255)
                                                                                                                       aGGCCGCCAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTgg                            >  2:595729/1‑151 (MQ=255)
                                                                                                                         gccgccAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGgcg                          >  1:5410287/1‑151 (MQ=255)
                                                                                                                           cgccAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGcc                        >  2:2407909/1‑151 (MQ=255)
                                                                                                                            gccAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCa                       <  1:230397/151‑1 (MQ=255)
                                                                                                                             ccAGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAg                      <  1:1356367/151‑1 (MQ=255)
                                                                                                                               aGCGCCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAgcg                    <  2:2877987/151‑1 (MQ=255)
                                                                                                                                gcgcCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAgcgc                   >  1:2801723/1‑151 (MQ=255)
                                                                                                                                  gcCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGcgcgc                 >  2:4426828/1‑150 (MQ=255)
                                                                                                                                  gcCTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGa                 >  2:499552/1‑151 (MQ=255)
                                                                                                                                   ccTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGat                <  1:6165504/151‑1 (MQ=255)
                                                                                                                                   ccTGGCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGat                >  2:2598198/1‑151 (MQ=255)
                                                                                                                                     ggggagtCGTGCGCCGGGGGCGCCCGGGGTTGCGGATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGatat              <  2:6479351/145‑1 (MQ=255)
                                                                                                                                      ggCATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGCTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATc             >  1:6512957/1‑151 (MQ=255)
                                                                                                                                        cATTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATccc           <  1:3930627/151‑1 (MQ=255)
                                                                                                                                         aTTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATcccc          <  1:1656155/151‑1 (MQ=255)
                                                                                                                                         aTTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATcccc          <  2:5788451/151‑1 (MQ=255)
                                                                                                                                         aTTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATcccc          >  2:4619598/1‑151 (MQ=255)
                                                                                                                                         aTTCGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATcccc          <  2:3780903/151‑1 (MQ=255)
                                                                                                                                            cGTGCGCCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATCCCCAAc       >  1:5267927/1‑151 (MQ=255)
                                                                                                                                               gcgcCAGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATCCCCAACAtt    <  1:311313/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTCACCCTCAAGGCCCGAATTACGCGCGCCGGCGACAGCTACCGCATAAGTGGCGAGAAGTTCTACTCCACCGGTGCCTTGTTCGCCCATTGGGTGGCGGTAAAAGCCCTCGACGACGAAGGCCGCCAGCGCCTGGCATTCGTGCGCCGGGGAAGCCCGGGGTTGCGCATTGTCGATGACTGGTCCGGGTTCGGCCAGCGCACCACCGCCAGCGGTACCGTGCTGCTGGACCAGGTGCCGGTTGACGCGGAACTGGTGATCGACAACTGGCGCCAGCGCGATATCCCCAACATTCA  >  CP009974/1844639‑1844934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑