BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009974	1,915,245	A→G	noncoding (50/116 nt)	RPPX_08540 ←	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009974	1,915,245	0	A	G	93.0%	470.9 / 12.6	186	noncoding (50/116 nt)	RPPX_08540	5S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (5/8); new base G (97/75); total (103/83)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

AGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTTGCCAGAATGTCCAGATGGACAGCCCAGCGAATCCGGATATGTGATGTTTGCGAAGCTCTTGTGTAAAGCACGAATTTTCGGGTCTTTC  >  CP009974/1915097‑1915392

aGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCActgctg                                                                                                                                                  <  1:3287596/151‑1 (MQ=21)

  cTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAg                                                                                                                                                >  1:796434/1‑151 (MQ=21)

  cTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAg                                                                                                                                                <  1:2575458/151‑1 (MQ=21)

   tATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGt                                                                                                                                               >  2:3449864/1‑151 (MQ=21)

    aTCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGtt                                                                                                                                              >  1:72938/1‑151 (MQ=21)

     tCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATAGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTc                                                                                                                                             <  1:598557/151‑1 (MQ=18)

      cAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCg                                                                                                                                            >  1:2968509/1‑151 (MQ=18)

      cAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCg                                                                                                                                            >  1:3630251/1‑151 (MQ=21)

       aGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgg                                                                                                                                           <  2:73172/151‑1 (MQ=18)

        gTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCggg                                                                                                                                          <  2:4433660/151‑1 (MQ=18)

         ttCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCggga                                                                                                                                         >  2:393663/1‑151 (MQ=18)

          tCTAACCGATATTGAAACCCGTGAAAGACAACGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggat                                                                                                                                        <  2:4636445/151‑1 (MQ=18)

          tCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggat                                                                                                                                        >  2:2046247/1‑151 (MQ=18)

          tCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggat                                                                                                                                        <  2:2322556/151‑1 (MQ=18)

           cTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggttg                                                                                                                                       >  1:2412721/1‑151 (MQ=18)

           cTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggatg                                                                                                                                       <  1:850386/151‑1 (MQ=18)

           cTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggatg                                                                                                                                       <  1:1178540/151‑1 (MQ=18)

            tAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCgggatgg                                                                                                                                      <  2:6111055/151‑1 (MQ=18)

             aaCCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgggatggg                                                                                                                                     <  2:5321699/151‑1 (MQ=18)

              aCCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCgggatggga                                                                                                                                    <  2:4213362/151‑1 (MQ=18)

                  atatTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAg                                                                                                                                >  2:4853905/1‑151 (MQ=18)

                  atatTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAg                                                                                                                                <  2:4354434/151‑1 (MQ=17)

                    atTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAggt                                                                                                                              >  1:2198239/1‑151 (MQ=17)

                      tGAATCCCGTGAAAGACAACGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAggtgg                                                                                                                            <  2:3281664/151‑1 (MQ=17)

                      tGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAggtgg                                                                                                                            <  2:5175107/151‑1 (MQ=17)

                        aataccGTGAGAGACAAAGCCCCTGTGTGCAGGGCAGACGGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGtt                                                                                                                          <  2:6007521/147‑1 (MQ=14)

                         aaCCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTc                                                                                                                         >  2:2094405/1‑151 (MQ=17)

                         aaCCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTc                                                                                                                         <  1:2046247/151‑1 (MQ=17)

                          aCCCGTTAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTcc                                                                                                                        <  1:2436106/151‑1 (MQ=14)

                          aCCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTcc                                                                                                                        <  1:6264891/151‑1 (MQ=17)

                          aCCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTcc                                                                                                                        <  1:3449864/151‑1 (MQ=17)

                            ccGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCaa                                                                                                                      <  1:147716/151‑1 (MQ=17)

                             cGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAAt                                                                                                                     <  2:876776/151‑1 (MQ=17)

                              gTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATg                                                                                                                    <  2:788174/151‑1 (MQ=14)

                               tGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGc                                                                                                                   <  2:195283/151‑1 (MQ=14)

                                gAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGct                                                                                                                  >  1:4978716/1‑151 (MQ=14)

                                 aaaGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGctc                                                                                                                 >  1:6457812/1‑151 (MQ=14)

                                  aaGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGctct                                                                                                                <  2:372733/151‑1 (MQ=14)

                                  aaGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGctct                                                                                                                >  1:5644347/1‑151 (MQ=14)

                                   aGACAAAGCCCCTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCCCTa                                                                                                               >  1:1667129/1‑151 (MQ=14)

                                   aGACAAAGCCCCTGTCAGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTa                                                                                                               <  2:1831899/151‑1 (MQ=14)

                                        aaGCCCCTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGgtc                                                                                                          >  2:4101266/1‑151 (MQ=14)

                                              cTGTCTGCATCGCAGACAGGGGCTTTGGG‑TTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCACTATGGTCGTCAAg                                                                                                    >  1:6335187/1‑151 (MQ=14)

                                              cTGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAg                                                                                                    >  2:3667526/1‑151 (MQ=14)

                                               tGTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGa                                                                                                   >  2:256437/1‑151 (MQ=14)

                                                gTCTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGaa                                                                                                  >  2:148565/1‑151 (MQ=11)

                                                  cTGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAAt                                                                                                <  2:6475439/151‑1 (MQ=11)

                                                   tGCATCGCATACAGGGGCTTTG‑GGTTTGAATCTTGACCATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAAtt                                                                                               <  2:5126432/151‑1 (MQ=11)

                                                   tGCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGACAtt                                                                                               >  2:5058423/1‑151 (MQ=11)

                                                    gCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTc                                                                                              <  2:6292666/151‑1 (MQ=11)

                                                    gCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTc                                                                                              <  1:1853456/151‑1 (MQ=11)

                                                    gCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTc                                                                                              <  2:5100716/151‑1 (MQ=11)

                                                    gCATCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTc                                                                                              <  1:6053867/151‑1 (MQ=11)

                                                      aTCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTc                                                                                              >  1:2327629/1‑149 (MQ=11)

                                                       tCGCAGACAGGGGCTTTG‑GGTTTGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGt                                                                                           >  1:570204/1‑151 (MQ=11)

                                                        cGCAGACAGGGGCTTTGGGTT‑TGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTg                                                                                          >  1:1756024‑M2/1‑150 (MQ=255)

                                                                       ttgggtttgaatcttGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcac                                                                            >  2:4453166‑M2/8‑136 (MQ=255)

                                                                           gtttgaaTCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactagg                                                                        >  2:5857299‑M2/4‑132 (MQ=255)

                                                                             ttgAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggac                                                                      >  2:3354744‑M2/2‑130 (MQ=255)

                                                                              tGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacg                                                                     >  1:6433796‑M2/1‑129 (MQ=255)

                                                                               gAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgc                                                                    >  1:2187344‑M2/1‑128 (MQ=255)

                                                                                 aTCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgc                                                                  >  1:975790‑M2/1‑126 (MQ=255)

                                                                                 aTCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGGGGTTCCACTGCTCTATGGTCGTCAAGAAATTCTGTgtgcgggccagtcacttggacgcca                                                                  <  1:4867843‑M2/151‑26 (MQ=255)

                                                                                  tCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcc                                                                 >  2:2334725‑M2/1‑125 (MQ=255)

                                                                                  tCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcc                                                                 >  1:3648598‑M2/1‑125 (MQ=255)

                                                                                        cGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcgcgtcactaggacgcgcctgcgaa                                                           <  1:3992417‑M2/151‑33 (MQ=255)

                                                                                        cGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaa                                                           <  1:3320875‑M2/151‑33 (MQ=255)

                                                                                        cGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaa                                                           >  1:4487903‑M2/1‑119 (MQ=255)

                                                                                         gATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaat                                                          >  2:268850‑M2/1‑118 (MQ=255)

                                                                                         gATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaat                                                          <  1:3350866‑M2/151‑34 (MQ=255)

                                                                                          aTGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatc                                                         <  1:5782983‑M2/151‑35 (MQ=255)

                                                                                              ccTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctg                                                     >  2:3868442‑M2/1‑113 (MQ=255)

                                                                                              ccTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctg                                                     >  1:6114591‑M2/1‑113 (MQ=255)

                                                                                               cTACTCGCACGTAGAGAACCCCCACACTGCCGTCGGCGATGCATCGTTTGACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatccctgt                                                    >  2:5384370‑M2/1‑112 (MQ=255)

                                                                                                 aCTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtag                                                  >  2:2641258‑M2/1‑110 (MQ=255)

                                                                                                      cacaTGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcct                                             >  2:2198239‑M2/1‑105 (MQ=255)

                                                                                                        caTGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctac                                           <  1:105339‑M2/151‑49 (MQ=255)

                                                                                                           ggggAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctactta                                        >  1:4151154‑M2/1‑100 (MQ=255)

                                                                                                            gggAAACCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaa                                       >  1:1517101‑M2/1‑99 (MQ=255)

                                                                                                               aaaCCCCACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaaga                                    >  1:5512814‑M2/1‑96 (MQ=255)

                                                                                                                   cccACACTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaa                                >  2:2305638‑M2/1‑92 (MQ=255)

                                                                                                                     cacaCTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaac                              <  1:4107018‑M2/151‑62 (MQ=255)

                                                                                                                         cTACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccct                          <  1:4234914‑M2/151‑66 (MQ=255)

                                                                                                                          tACCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaaccccta                         >  2:3629810‑M2/1‑85 (MQ=255)

                                                                                                                           aCCATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctac                        >  2:3661826‑M2/1‑84 (MQ=255)

                                                                                                                             cATCGGCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacct                      >  2:239538‑M2/1‑82 (MQ=255)

                                                                                                                                  gCGATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcata                 >  2:5471262‑M2/1‑77 (MQ=255)

                                                                                                                                    gATGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacg               <  1:347980‑M2/151‑77 (MQ=255)

                                                                                                                                     aTGCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgc              >  2:4532411‑M2/1‑74 (MQ=255)

                                                                                                                                       gCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcag            >  1:5134600‑M2/1‑72 (MQ=255)

                                                                                                                                       gCATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTGGCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaacacccctacctgcatacgcag            >  2:3590313‑M2/1‑72 (MQ=255)

                                                                                                                                        cATCGTTTCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaacacccctacctgcatacgcaga           <  2:2723101‑M2/151‑81 (MQ=255)

                                                                                                                                             tttCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggctcgtcactaggacgcgcctgcgaatccctgtaggtcctgcttacagacaaaacctctacctgcatactccgataggg      <  2:1991175‑M2/151‑86 (MQ=255)

                                                                                                                                             tttCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtaggtcctgcttaaagacaaaacctctaccggcagacgcagataggg      <  1:5037136‑M2/151‑86 (MQ=255)

                                                                                                                                              ttCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatagggg     >  1:1020488‑M2/1‑65 (MQ=255)

                                                                                                                                              ggcacTGCTGGGTGCGGAATGGGATCAGGTGGTTCCAATGCTCTGGGGTCGGCAAGAAATTCTGTgtgctggcccgtcactaggacacgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagatagggg     >  2:3199355‑M2/3‑65 (MQ=255)

                                                                                                                                               tCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggt    >  2:426878‑M2/1‑64 (MQ=255)

                                                                                                                                               tCACTGCTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggt    >  1:1634824‑M2/1‑64 (MQ=255)

                                                                                                                                                cACTGCAGAGATCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAAATCTGTgtgctggcacgtcactaggacgcgccagcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggtt   >  2:2968509‑M2/1‑63 (MQ=255)

                                                                                                                                                 actgctGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTgtgctggcccgtcactaggacgcgcctgcgaatctctgtagttcctacttaaagacaaaacccctacctgcatacgcagataggggttt  >  2:5690625‑M2/1‑62 (MQ=255)

AGCTATCAGTTCTAACCGATATTGAAACCCGTGAAAGACAAAGCCCCTGTCTGCATCGCAGACAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACTACCATCGGCGATGCATCGTTTCACTACTGAGTTCGGGATGGGATCAGGTGGTTCCAATGCTCTATGGTCGTCAAGAAATTCTGTTGCCAGAATGTCCAGATGGACAGCCCAGCGAATCCGGATATGTGATGTTTGCGAAGCTCTTGTGTAAAGCACGAATTTTCGGGTCTTTC  >  CP009974/1915097‑1915392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`