Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009974 2,331,550 C→T Q88Q (CAG→CAA)  RPPX_10445 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0099742,331,5500CT99.8% 1489.3 / NA 396Q88Q (CAG→CAA) RPPX_10445membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (205/190);  minor base G (1/0);  total (206/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 396 total aligned reads displayed.

GGCCTATGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCCTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCGGCTGCC  >  CP009974/2331402‑2331697
                                                                                                                                                    |                                                                                                                                                   
ggCCTATGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTg                                                                                                                                                   <  2:2993877/151‑1 (MQ=255)
   cTATGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTc                                                                                                                                                <  1:673880/151‑1 (MQ=255)
   cTATGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTc                                                                                                                                                <  2:2717268/151‑1 (MQ=255)
    tATGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTcc                                                                                                                                               <  1:1358085/151‑1 (MQ=255)
     aTGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCa                                                                                                                                              >  1:4281569/1‑151 (MQ=255)
      tGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAg                                                                                                                                             <  1:5945780/151‑1 (MQ=255)
       gTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAgg                                                                                                                                            >  1:2127203/1‑151 (MQ=255)
        tAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGgc                                                                                                                                           <  2:5682939/151‑1 (MQ=255)
        tAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGgc                                                                                                                                           >  1:4459261/1‑151 (MQ=255)
             ggCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGc                                                                                                                                      >  2:5888638/1‑151 (MQ=255)
             ggCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGGTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGGATTGGCCGCGGTCCGTGGCGTAGTCCAGGCGCTGc                                                                                                                                      >  2:1459710/1‑151 (MQ=255)
              gcgcCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCg                                                                                                                                     >  2:1792132/1‑151 (MQ=255)
                    aTGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCCCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTCGGTCCAGGCGCTGCGGAGTTa                                                                                                                               >  2:2278423/1‑151 (MQ=255)
                      gAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGc                                                                                                                             >  2:4325874/1‑151 (MQ=255)
                       aaCGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCg                                                                                                                            >  2:2541473/1‑151 (MQ=255)
                          gCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCa                                                                                                                         <  1:3264722/151‑1 (MQ=255)
                           cTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCAc                                                                                                                        >  1:2474549/1‑151 (MQ=255)
                               gCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACggcg                                                                                                                    >  2:3675446/1‑150 (MQ=255)
                                cTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACggctg                                                                                                                   >  1:4967413/1‑151 (MQ=255)
                                 tCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACggctgg                                                                                                                  >  1:3827576/1‑151 (MQ=255)
                                 tCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACggctgg                                                                                                                  <  2:1054559/151‑1 (MQ=255)
                                  cATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACggctggc                                                                                                                 <  1:529094/151‑1 (MQ=255)
                                    tGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAg                                                                                                                              <  1:2269044/136‑1 (MQ=255)
                                    tGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGgctggctg                                                                                                               >  2:523526/1‑151 (MQ=255)
                                     ggCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCTCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTTGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGctggctgg                                                                                                              >  1:3104213/1‑151 (MQ=255)
                                     ggCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGc                                                                                                                             >  2:3293236/1‑136 (MQ=255)
                                     ggCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGctggctgg                                                                                                              >  1:4863491/1‑151 (MQ=255)
                                      gCCGGGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGa                                                                                                             <  2:5912680/151‑1 (MQ=255)
                                       ccGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGaa                                                                                                            >  1:1978732/1‑151 (MQ=255)
                                         gTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACt                                                                                                          <  2:391636/151‑1 (MQ=255)
                                         gTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACt                                                                                                          <  1:984415/151‑1 (MQ=255)
                                            gCATCGAGATTGATATGCAGTTCAGGCGAGCGGCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCgg                                                                                                       <  2:2302801/151‑1 (MQ=255)
                                             cATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCggg                                                                                                      <  1:5744517/151‑1 (MQ=255)
                                              aTCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGc                                                                                                     >  1:2326692/1‑151 (MQ=255)
                                              aTCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGc                                                                                                     <  1:1115767/151‑1 (MQ=255)
                                                cGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACCTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGACTGGCTGGAACTCGGGCgg                                                                                                   >  2:700034/1‑151 (MQ=255)
                                                 gACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGt                                                                                                  <  1:5030837/151‑1 (MQ=255)
                                                       gATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAgg                                                                                            <  1:3577472/151‑1 (MQ=255)
                                                           tGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCtt                                                                                        <  1:4009626/151‑1 (MQ=255)
                                                            gCAGTTCCGGCGAGCGCGCGGCGAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTc                                                                                       <  2:5455002/151‑1 (MQ=255)
                                                            gCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTc                                                                                       >  2:6016631/1‑151 (MQ=255)
                                                            gCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTc                                                                                       <  2:1297789/151‑1 (MQ=255)
                                                                ttCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTg                                                                                   <  2:6167024/151‑1 (MQ=255)
                                                                  ccGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAg                                                                                 <  1:3859084/151‑1 (MQ=255)
                                                                     gCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCtt                                                                              >  1:2877742/1‑151 (MQ=255)
                                                                     gCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCtt                                                                              <  2:5799891/151‑1 (MQ=255)
                                                                       gAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTgc                                                                            >  2:5215492/1‑151 (MQ=255)
                                                                       gAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTgc                                                                            <  2:286136/151‑1 (MQ=255)
                                                                          cgcCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGcgcg                                                                         >  2:4965452/1‑151 (MQ=255)
                                                                           gcCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCga                                                                        <  1:4325874/151‑1 (MQ=255)
                                                                             cgggCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCgaga                                                                      <  2:2206331/149‑1 (MQ=255)
                                                                                 ccAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAg                                                                  <  1:792906/151‑1 (MQ=255)
                                                                                 ccAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAg                                                                  <  2:512608/151‑1 (MQ=255)
                                                                                    gcagGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCt                                                               <  1:3748994/151‑1 (MQ=255)
                                                                                     ccggTCGCTCTGGAAGTTGACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTGCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTa                                                              <  2:2015452/149‑1 (MQ=255)
                                                                                        gTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATgg                                                           >  2:3535408/1‑151 (MQ=255)
                                                                                        gTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATgg                                                           >  2:4794073/1‑151 (MQ=255)
                                                                                         tCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGt                                                          <  2:307540/151‑1 (MQ=255)
                                                                                         tCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGt                                                          <  2:3775235/151‑1 (MQ=255)
                                                                                         tCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGt                                                          >  2:1743657/1‑151 (MQ=255)
                                                                                             tctGGAAGTTCACCGGCACGTTGATGGCGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTc                                                      <  2:6415925/151‑1 (MQ=255)
                                                                                             tctGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTc                                                      >  1:6247483/1‑151 (MQ=255)
                                                                                              ctGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCCGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCa                                                     >  1:5159322/1‑151 (MQ=255)
                                                                                                 gAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATcc                                                  <  1:4245324/151‑1 (MQ=255)
                                                                                                  aaGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATcca                                                 >  1:268107/1‑151 (MQ=255)
                                                                                                   aGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATccac                                                >  1:6307365/1‑151 (MQ=255)
                                                                                                    gTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATccacc                                               <  2:2440131/151‑1 (MQ=255)
                                                                                                       cACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCaccacc                                            >  2:1617193/1‑151 (MQ=255)
                                                                                                        aCCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCg                                           >  1:5487039/1‑151 (MQ=255)
                                                                                                        aCCGGCACGTAGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCg                                           <  1:2156684/151‑1 (MQ=255)
                                                                                                          cGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGcc                                         >  1:3402981/1‑151 (MQ=255)
                                                                                                           ggCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCa                                        >  2:1968819/1‑151 (MQ=255)
                                                                                                           ggCACGTGGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCa                                        <  2:4863491/151‑1 (MQ=255)
                                                                                                            gCACGGGGATGGCGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCAc                                       <  2:4121359/151‑1 (MQ=255)
                                                                                                               cGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCt                                    >  2:3251776/1‑151 (MQ=255)
                                                                                                                gTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTg                                   <  2:3597357/151‑1 (MQ=255)
                                                                                                                gTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTg                                   >  1:2780437/1‑151 (MQ=255)
                                                                                                                gTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTg                                   >  1:158335/1‑151 (MQ=255)
                                                                                                                  tgatgaCGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGc                                 >  2:423067/1‑151 (MQ=255)
                                                                                                                  ggatgaCGAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGACGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGc                                 <  2:6278474/150‑1 (MQ=255)
                                                                                                                         gAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTgtgt                          >  2:2611405/1‑151 (MQ=255)
                                                                                                                         gAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTgtgt                          <  1:6059744/151‑1 (MQ=255)
                                                                                                                         gAAGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTgtgt                          >  1:1761127/1‑151 (MQ=255)
                                                                                                                           aGGTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTAc                        <  1:46700/151‑1 (MQ=255)
                                                                                                                            ggTGTATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTg                                                                             <  2:2486096/97‑1 (MQ=255)
                                                                                                                               gtATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCg                    >  1:1083878/1‑151 (MQ=255)
                                                                                                                                tATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGa                   >  2:1433403/1‑151 (MQ=255)
                                                                                                                                tATTGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGa                   >  1:5860754/1‑151 (MQ=255)
                                                                                                                                  ttGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATc                 >  2:1641584/1‑151 (MQ=255)
                                                                                                                                  ttGGCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATc                 >  1:3701255/1‑151 (MQ=255)
                                                                                                                                    ggCCGCTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCAc               >  1:4184852/1‑151 (MQ=255)
                                                                                                                                      ccgccgCCCATGGCTTGGTCCAGGCGCTGCCGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTACGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCg             >  2:2091228/1‑151 (MQ=255)
                                                                                                                                         cTGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGc          >  2:5822008/1‑151 (MQ=255)
                                                                                                                                          tGTCCATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGcc         <  1:1635771/151‑1 (MQ=255)
                                                                                                                                           gTCCATGGCTTGGTCCAGGCGCTGCGGGGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCg        <  2:3838594/151‑1 (MQ=255)
                                                                                                                                              cATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCGGCt     <  1:3243495/151‑1 (MQ=255)
                                                                                                                                              cATGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCGGCt     <  1:1474592/151‑1 (MQ=255)
                                                                                                                                               aTGGCTTGGTCCAGGCGGTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCGGCTg    <  2:4455788/151‑1 (MQ=255)
                                                                                                                                               aTGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCGGCTg    >  1:5383388/1‑151 (MQ=255)
                                                                                                                                               aTGGCTTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTCGTGTACGCCGATCACCGAGCCGGCTg    >  2:5301817/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCTATGTAGCCGGCGCCCATGAACGCTTGGCTCATGGCCGTGGCATCGACATTGATCTGCAGTTCCGGCGAGCGCCCGGCCAGCAGGTCGCTCTGGAAGTTCACCGGCACGTTGATGACGAAGGTGTATTGGCCGCTGTCCATGGCCTGGTCCAGGCGCTGCGGAGGTAGCGGCACGGCTGGCTGGAACTCGGGCGGTTGCAGGGCTTCGCTGAGCTTGCGCGAGAGCAGGCTATGGTCTTCATCCACCACCGCCACGCTGGCGTTGTGTACGCCGATCACCGAGCCGGCTGCC  >  CP009974/2331402‑2331697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑