Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000962,947,4670AC23.8% 93.8 / 18.6 42intergenic (+4/‑30)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (12/20);  new base C (4/6);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCA  >  U00096/2947319‑2947577
                                                                                                                                                    |                                                                                                               
aaaTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                 >  2:1672308/1‑149 (MQ=255)
 aaTCCGTGATCGTGCTAAAATACAGGTTGCACGAAAACGCCAGCGTAGTATAGAGCGACTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                                                                                                <  1:1672278/149‑1 (MQ=17)
 aaTCCGTGATCGGGGTAAAAAATAGGTTGCATGAAATCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTTGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaa                                                                                                                <  2:1672277/149‑1 (MQ=255)
  aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                               <  1:1672279/149‑1 (MQ=255)
    ccGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt                                                                                                             <  1:1672283/149‑1 (MQ=255)
    ccGGGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCAGCCCCGGACGCGGGGTGGAGCAGCCGGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt                                                                                                             <  2:1672281/149‑1 (MQ=255)
     cGTGATCGTGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACACGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAtt                                                                                                            <  1:1672284/149‑1 (MQ=255)
       tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                                                          >  2:1672287/1‑149 (MQ=255)
       tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                                                          <  1:1672287/149‑1 (MQ=255)
       tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                                         >  2:1672333/1‑149 (MQ=255)
        gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                                         <  2:1672289/149‑1 (MQ=255)
        gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                                                          <  1:1672289/148‑1 (MQ=255)
        gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                                                               >  1:1672296/1‑143 (MQ=255)
        gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgat                                                                                                        >  1:1672290/1‑149 (MQ=255)
           cGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGaa                                                                                                     <  2:1672291/149‑1 (MQ=255)
              ggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                                                   <  2:1672293/149‑1 (MQ=255)
              ggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                                                   <  2:1672292/149‑1 (MQ=255)
               gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑a                                                                                                 <  1:1672293/149‑1 (MQ=255)
                 aaaataaaGGTTGCATGAAAACTCGAGCGTAGTATAGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaa                                                                                              <  2:1672297/149‑2 (MQ=17)
                   aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaagc                                                                                            >  2:1672331/1‑146 (MQ=255)
                    aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                                         <  1:1672299/136‑1 (MQ=255)
                    aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                                         >  2:1672300/1‑136 (MQ=255)
                       aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTa                                                                                         <  1:1672300/149‑1 (MQ=255)
                       aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAATTTTG‑TGAAGTAAAAGCAGTa                                                                                         >  1:1672353/1‑149 (MQ=18)
                         ggTTGAATGAAAACTCTAGAGTAGTATAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaat                                                                                                              <  2:1672302/127‑2 (MQ=14)
                          gTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAAtt                                                                                                            >  1:1672301/1‑127 (MQ=255)
                           ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGt                                                                                     >  2:2424255/1‑149 (MQ=255)
                           ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGt                                                                                     <  2:1672303/149‑1 (MQ=255)
                           ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGt                                                                                     <  2:1672304/149‑1 (MQ=255)
                             gaaTGAATAAGCTAGCGGAGTATAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGa                                                                                   <  2:1672354/147‑1 (MQ=11)
                             gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGa                                                                                   <  1:1672304/149‑1 (MQ=255)
                               aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCTGGCTCATAAACCGAAGGTCGACGGTTCAAATCCGGCCCCCGCAACCAATTAAAAGTTGATAAAGT‑AAAGCAGTACGGTGCcg                                                                                 >  1:1672337/1‑149 (MQ=21)
                               aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGAc                                                                                  <  1:1672307/148‑1 (MQ=255)
                                tGAAAACGCGAGCGGAGTATAGTGCGATTCCCGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTGAcgc                                                                                <  2:1672352/149‑1 (MQ=11)
                                    aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCgggg                                                                            <  2:1672312/149‑1 (MQ=255)
                                       gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑ATAGCAGTACGGTGACGCGGGGTgg                                                                         <  2:1672313/149‑1 (MQ=255)
                                         gAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGag                                                                       <  2:1672314/149‑1 (MQ=255)
                                           ccGGAGTATAGTGCGCATCCACGTCAGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGTATCATAACCCTAATGTCGTCGGGGCTAATCCGGCCGCCGCAACCAATTAAATATTGCTGAAGT‑AACGCATTACTGGGACGCGGGGTGGagca                                                                     <  2:1672315/148‑1 (MQ=17)
                                              gAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACCAATTAAAATTTGCTGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                  <  1:1672316/149‑1 (MQ=255)
                                                 tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                 >  1:1672379/1‑100 (MQ=255)
                                                          cATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                               >  1:1672352/1‑93 (MQ=255)
                                                                   aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATa                                             <  1:1672321/149‑1 (MQ=40)
                                                                                                  ggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTAATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcg                                                        >  2:1672362/1‑107 (MQ=255)
                                                                                                   gCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                    >  2:1672324/1‑110 (MQ=255)
                                                                                                   gCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCgg                                                    <  1:1672323/110‑1 (MQ=255)
                                                                                                                               aTCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCt                                                          >  1:2301509/1‑76 (MQ=255)
                                                                                                                                            aaCCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCgg                             >  1:1672371/1‑92 (MQ=255)
                                                                                                                                                  ttAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCaa     >  1:1672363/1‑110 (MQ=38)
                                                                                                                                                  ttAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtta  >  2:1672368/1‑111 (MQ=25)
                                                                                                                                                   tAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                   >  2:1672369/1‑95 (MQ=38)
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AAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCA  >  U00096/2947319‑2947577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑