Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG23.8% 251.3 / 14.7 122intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (12/81);  new base G (25/4);  total (37/85)
Fisher's exact test for biased strand distribution p-value = 3.97e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 123 total aligned reads displayed.

TGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAC  >  U00096/422288‑422575
                                                                                                                                             |                                                                                                                                                  
tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcctc                                                                                                                                             >  2:245819/1‑149 (MQ=255)
    ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGccccc                                                                                                                                         >  1:245824/1‑149 (MQ=255)
     tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTCCGCCCCCa                                                                                                                                        >  2:245800/1‑149 (MQ=255)
      gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGccccccc                                                                                                                                       >  2:245782/1‑147 (MQ=255)
       ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                      <  1:245733/149‑1 (MQ=255)
       ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGGCCTCCTCCCCCaaa                                                                                                                                      >  1:245839/1‑149 (MQ=255)
            cgggtgcttGGCTTGATTTTTAAACGCCGCCACGTTCGTCCTCTGGCTGTAAACCAGTTATTCTTGCGGTCTTTTAGCCGGGTGATGCGGTAATCCTGTTCCGGGTATTCCGTAAGTTTTCCGCCCCCGGCCTCCTCCCCCAAATATcc                                                                                                                                 <  2:841843/142‑1 (MQ=255)
                 gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                             <  1:245735/148‑1 (MQ=255)
                  aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                           <  2:245737/149‑1 (MQ=255)
                   ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                          <  1:245737/149‑1 (MQ=255)
                           aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                  <  2:245739/149‑1 (MQ=255)
                             gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                <  2:245741/149‑1 (MQ=255)
                              tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                               <  1:245741/149‑1 (MQ=255)
                                   aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTCCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                          <  2:245743/149‑1 (MQ=255)
                                       cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                       <  1:245743/148‑1 (MQ=255)
                                        gccACGATCGTCAGCTGGCTGAAAACCAGTACTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                     <  1:245744/149‑1 (MQ=255)
                                         ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                    <  1:1903593/149‑1 (MQ=255)
                                         ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                    <  1:245745/149‑1 (MQ=255)
                                          cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                   <  2:2214312/149‑1 (MQ=255)
                                          cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                   <  2:245748/149‑1 (MQ=255)
                                             gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                <  1:245748/149‑1 (MQ=255)
                                              aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCCGCCCCCAAAAATCCTGCAGAGGTGGAGTGATGGGGCATTAGGGTATgg                                                                                               >  2:245830/1‑149 (MQ=255)
                                              aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                               <  1:2061955/149‑1 (MQ=255)
                                              aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATACTGCATTAGGCTATgg                                                                                               >  2:245864/1‑149 (MQ=255)
                                               tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                              <  1:245749/149‑1 (MQ=255)
                                                cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                             <  2:245752/149‑1 (MQ=255)
                                                 gTCAGCTGGCTGTAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                            <  1:245752/149‑1 (MQ=255)
                                                 gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                            <  2:245756/149‑1 (MQ=255)
                                                 gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                            <  2:245754/149‑1 (MQ=255)
                                                 gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                            <  1:245756/149‑1 (MQ=255)
                                                 gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                            <  1:245757/149‑1 (MQ=255)
                                                 gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                            <  2:245755/149‑1 (MQ=255)
                                                    aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGCGGGCCGCCTCCCCCCAAAATCCTTCAGATGGTGAGTGAGCCTGCATTAGGCTAgggggaggg                                                                                         >  2:245837/1‑143 (MQ=255)
                                                     gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                          <  1:245760/147‑1 (MQ=255)
                                                       tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                      <  2:245764/149‑1 (MQ=255)
                                                       tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                      <  2:245762/149‑1 (MQ=255)
                                                       tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                      <  1:245762/149‑1 (MQ=255)
                                                        ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                     <  1:245765/149‑1 (MQ=255)
                                                        ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                     <  1:245764/149‑1 (MQ=255)
                                                          ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCGCGGCCTCCGCCCCCAAATAACCATCAGATGAGGAGGGATGCTGCATTAGGGGATGGGAAGGTGATGAg                                                                                   >  2:1317010/1‑149 (MQ=255)
                                                           tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTCTCCGACCCCGGCCGCCTACCCCCAAAATCAATCAGATAGTGTGTGATCAAGCATTAGGCGATGGCCAGGtcggc                                                                                     >  2:245818/1‑142 (MQ=255)
                                                           tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCTCCCCCAAATATCCTGCAGATGATGAGTGGTGCTGCATTAGGCGATGGGAAGGGGATc                                                                                     >  2:245820/1‑146 (MQ=255)
                                                           tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGATTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                    <  1:245767/147‑1 (MQ=255)
                                                           tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                  <  2:245770/149‑1 (MQ=255)
                                                           tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                  <  1:245768/149‑1 (MQ=255)
                                                               aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGGCGCCCCCCCAGATCAGGCAGAAGAGGCGTGGTGCGGCGGTAGGCTATGGGAAGGGGAGAAGAtttt                                                                              >  2:245828/1‑149 (MQ=255)
                                                               aaCCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                              <  2:245771/149‑1 (MQ=255)
                                                                 ccAGTAATCCTTGCGGTCTGTAAGCCGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                             <  1:245771/148‑1 (MQ=255)
                                                                  cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCGCCCCAAGATACGGCAGAGGAGGAGTGAGACGGCAGTAGGCTAGTGGAGGGTGAGCAGGTTTtcat                                                                           >  2:245833/1‑149 (MQ=255)
                                                                   aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                          <  1:245774/149‑1 (MQ=255)
                                                                         ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCTCCCCCCAATATCATTGAGATGATGTGTGATACTGCATTAGGGTATGGCAAGGTGGTCAGATTTTGATCGCAggg                                                                    >  2:245832/1‑149 (MQ=255)
                                                                          cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCGCCTCCCCCAGCGAGGCGTCAGAGGATGAGGGGTCCGGGGGTGGGGTATGGGAAGGGGAGGAGAGGTTGGTCACAgggg                                                                   >  2:245878/1‑149 (MQ=255)
                                                                           ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATATACTTGAGATGATGAGTGGTCCTGCGTTAGGGGTTGGGAGGGTGGTCAGCTTTTGATCACAggggg                                                                  >  2:245889/1‑148 (MQ=255)
                                                                           ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGATGATGAGGGATACGGCATTAGGCTATGGCAAGGTGATCAGATTTTCATGACAGGGGa                                                                  >  2:245866/1‑149 (MQ=255)
                                                                              cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                               <  1:245776/149‑1 (MQ=255)
                                                                               gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                              <  1:245777/149‑1 (MQ=255)
                                                                                ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCGGCGTTAGGGTATGGCAAGGTGCTGAGATTTTGATCACAGGGGAATTat                                                             >  2:360780/1‑149 (MQ=255)
                                                                                ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                <  1:245778/146‑1 (MQ=255)
                                                                                ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                             <  2:245780/149‑1 (MQ=255)
                                                                                ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                             <  2:245781/149‑1 (MQ=255)
                                                                                 tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGGATTATGCTATGGCAAGGTGTTGAGATTTTGATAACAGGGGAATTatg                                                            >  2:245875/1‑149 (MQ=255)
                                                                                  cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                           <  1:245781/149‑1 (MQ=255)
                                                                                    gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                         <  2:245783/149‑1 (MQ=255)
                                                                                    gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTGCTGACCCAAAAATCAGGCAGAGGATTAGGGATGCGGCATGAGGCTGTGGCAGGGGGAGGAGTTTTTAGTCAAAGGGGGAGTatgatg                                                         >  2:245867/1‑149 (MQ=255)
                                                                                      aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                       <  2:245784/149‑1 (MQ=255)
                                                                                           ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                  <  1:245785/149‑1 (MQ=255)
                                                                                             ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                <  2:245788/149‑1 (MQ=255)
                                                                                              tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                               <  2:245789/149‑1 (MQ=255)
                                                                                              tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                               <  1:245789/149‑1 (MQ=255)
                                                                                               cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGACGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                              <  1:245790/149‑1 (MQ=255)
                                                                                                  gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                     <  1:245791/139‑1 (MQ=255)
                                                                                                     gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAATTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                        <  2:245793/149‑1 (MQ=255)
                                                                                                      aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCGGCGGGGGCGGCCCCCAAAGAGCCTTCAGAAGATGAGTGATGATGAATGAGGGTATGGCAAGGTGATAAGATTTTAATAAAAGGGGAATTATGATGTTAAATTAATGGCaaatg                                       >  2:245861/1‑145 (MQ=255)
                                                                                                      aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                         <  1:245793/147‑1 (MQ=255)
                                                                                                         tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGGCCGCCGCCCCCAAAAATCATGCAGATGAGGAGGGATCGTGCATTAGGGTATGGCAAGGTAGTCAGATTTTAATAACAGGGGAATTATGATGTTAAATGAATGGCACCTGCCg                                    >  2:245853/1‑149 (MQ=255)
                                                                                                          ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                   <  1:245795/149‑1 (MQ=255)
                                                                                                           cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                  <  2:2399620/149‑1 (MQ=255)
                                                                                                           cTGATCCGGCTATTCCGAAAGTTATCCGACCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATTGCACCTGCCGGt                                  <  2:245797/149‑1 (MQ=255)
                                                                                                            tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                 >  1:245846/1‑149 (MQ=255)
                                                                                                             gATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGc                                <  1:245797/149‑1 (MQ=255)
                                                                                                                ccGGCTATTCCGAAAGTTATCCGCCCCCCGCCACCTCCCCCAAAAAACCATCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGGGATCAGATTTTAATCAAAGGGGAATTATTATGTTAAATTCATGGCACCTGCCGGGGcccc                             >  2:245848/1‑149 (MQ=255)
                                                                                                                  ggCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGAGGATGAGTGATCCTGAATTAGGGTATGGCAAGGTGATCAGATATTAATCACAGGGGAATTATGATGTTAAATTAATGGCACCAGCCGGTGCCCCCa                           >  2:245873/1‑149 (MQ=255)
                                                                                                                   gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                          <  1:245799/149‑1 (MQ=255)
                                                                                                                     tATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATACTTCAGATGATGAGTGTTGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGGGgcccccgtt                        >  2:245865/1‑145 (MQ=255)
                                                                                                                      aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                       <  2:245801/149‑1 (MQ=255)
                                                                                                                       ttCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAAAATGCATCAGATGATGAGTGATGCTGGATTAGGCTATGGCAAGGTGATCAGATTTTAATCACAGGGGAATTATGATGTTAAATTCAAGGCACCTGCCGGGGCCCCCATATGt                      >  2:245881/1‑149 (MQ=255)
                                                                                                                         ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                    <  2:245803/149‑1 (MQ=255)
                                                                                                                         ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                    <  1:245801/149‑1 (MQ=255)
                                                                                                                           gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                   <  1:245804/148‑1 (MQ=255)
                                                                                                                            aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                 <  1:245805/149‑1 (MQ=255)
                                                                                                                            aaaGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTGCAGATGATGAGTGGTACTGCAGTAGGGTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTTAATGAATGGCACCTGCCGGTGCCCCCAATTGTTAAAc                 >  2:245917/1‑149 (MQ=255)
                                                                                                                             aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                <  2:245807/149‑1 (MQ=255)
                                                                                                                                 tATCCGCGCCCGGCCGGCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGGAAGGGGATGAGATTTTAATCAAAGGGGAATTATGATGTTTAATGAATGGCACCTGCCGGTGCGCCCATTTGTTAAAcaaagc            >  2:245842/1‑149 (MQ=255)
                                                                                                                                 tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag             <  1:245807/148‑1 (MQ=255)
                                                                                                                                       cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa          >  1:245853/1‑145 (MQ=255)
                                                                                                                                       cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa       <  1:245809/148‑1 (MQ=255)
                                                                                                                                         cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa    <  2:245811/149‑1 (MQ=255)
                                                                                                                                         cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa    <  1:245811/149‑1 (MQ=255)
                                                                                                                                           cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc  <  2:245813/149‑1 (MQ=255)
                                                                                                                                           cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc  >  1:245907/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAC  >  U00096/422288‑422575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑