Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,708,7510AG22.5% 62.8 / 14.5 40E611E (GAA→GAG) rsxCSoxR [2Fe‑2S] reducing system protein RsxC
Reads supporting (aligned to +/- strand):  ref base A (8/23);  new base G (8/1);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.17e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG  >  U00096/1708605‑1708881
                                                                                                                                                  |                                                                                                                                  
aTCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAcc                                                                                                                                  <  2:945859/149‑1 (MQ=255)
   tcccGCGCCAAATCTCGCAATCTGGAACATCAACAGGCTAATGCGGAACCAGAACACCAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCaga                                                                                                                               <  2:945861/148‑1 (MQ=14)
   gCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTAGATCCGCACAGAGCCGCCGTCGAAGACGCTATTGCACGAGCAAAAGCCCGCAAGCTTTAAAAAAAACAGGCTAATGCGGAGcc                                                                                                                                  >  2:945893/1‑146 (MQ=11)
    cccGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACATGTCGATCCTCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCa                                                                                                                                 <  2:945862/146‑1 (MQ=255)
                cgcgcTTGCTGGAACAGCAACTCGCTACTGCGGAACCAGACCACCTGGTCGTTCCGCGCAATGCGGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                  <  2:2238088/149‑1 (MQ=14)
                     aaGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgcg                                                                                                             <  2:945866/149‑1 (MQ=255)
                           gAACAGCAACAGGCTACTGCGGACCCAGAACCCGAGGCCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATAGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgc                                                                                                       <  1:945867/149‑1 (MQ=14)
                           gAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgc                                                                                                       <  2:945869/149‑1 (MQ=255)
                              catcatcAGGCTAATGCGGAACCAGAACCAGTGGCCGATCCTCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccgc                                                                                                    <  2:945870/143‑1 (MQ=14)
                              cAGCATCAGGCTAATGCGGAACCAGAACCACAGGGCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccgc                                                                                                    <  2:945871/149‑1 (MQ=14)
                               agcttcAGGATACTTCGGAAGCAGAAACACTTGCCGATCCTCGTAAATCAGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccgcc                                                                                                   <  2:945882/144‑1 (MQ=11)
                                       gCTAATCCGGAACCAGCACCAGAGGCTGATCCTCGAAAATCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGc                                                                                           <  2:945886/149‑1 (MQ=14)
                                                           acaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCagag                                                                       >  2:945932/1‑149 (MQ=255)
                                                                 cGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                     <  1:945873/97‑1 (MQ=255)
                                                                  gATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                       <  1:945876/94‑1 (MQ=255)
                                                                  gATCCCCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaaga                                                                                                                            <  1:945875/89‑1 (MQ=255)
                                                                                 gccgTCGAAGCAGCTATAGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                       <  1:945884/79‑1 (MQ=11)
                                                                                            cGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAAAGTGGGATGCGCGCAAATCTGCCGTCGAGTCCGATTTTGCGCGAGCCAATGCCCGCATGGGGGTGAAGAAACAGGCTTATGcgg                                      >  1:945999/1‑147 (MQ=2)
                                                                                             gCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgcg                                                                                                             >  1:945940/1‑77 (MQ=255)
                                                                                               tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCa                                                                                                                                 >  2:945953/1‑55 (MQ=11)
                                                                                               tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGt                                                                                                                      >  1:946001/1‑66 (MQ=255)
                                                                                               tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCaga                                                                                                                               >  1:945953/1‑57 (MQ=255)
                                                                                                 ttGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGttg                                                                                                                    <  2:946002/66‑3 (MQ=255)
                                                                                                  agCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGAGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                                <  2:945890/148‑1 (MQ=255)
                                                                                                         gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg                                                                                         <  1:945891/85‑1 (MQ=33)
                                                                                                           cAAAGCGCGCAAGCTGGAACAGCAACAGTCTAATGCGTAAGCACAAGACCAGGGCGACCCGCGCTAAGCCGCGGTCTAAGCCGCTATTGCCCGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                       <  1:945892/149‑1 (MQ=14)
                                                                                                            aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATcc                                                                                                                >  2:945960/1‑59 (MQ=11)
                                                                                                            aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCAAGATCCAAAGCGCGAAATCTAGAAAAGCAACAAGCTAAAGCGGAGCCAGaacaca                          >  2:945961/1‑143 (MQ=11)
                                                                                                                 gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGc                                                                                        <  1:945893/78‑1 (MQ=21)
                                                                                                                   gcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCagaaga                                                                                                                            >  1:945925/1‑40 (MQ=255)
                                                                                                                   gcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAg                                                                                                                        >  2:945962/1‑44 (MQ=255)
                                                                                                                   gcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAg                                                                                                                        <  1:945961/44‑1 (MQ=255)
                                                                                                                   gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc                                                              <  1:945894/102‑1 (MQ=35)
                                                                                                                     aaGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg                                                                                                                        <  1:2708734/42‑1 (MQ=38)
                                                                                                                         tGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCcgcg                                                                                                             >  2:945964/1‑49 (MQ=11)
                                                                                                                                gCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGAAAGCTGGAACAGCATAAGGATAATTCCGATACAGAACATCCGGTATGACCGCGCAAAgcacacg  >  2:1797448/1‑144 (MQ=14)
                                                                                                                                     aGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTAGCCGCGGCTATTGCCCg                                                                               >  1:946027/1‑67 (MQ=11)
                                                                                                                                       gCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGc                                                                                           >  1:945967/1‑53 (MQ=255)
                                                                                                                                       gCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGc                                                                                           <  2:945968/53‑1 (MQ=255)
                                                                                                                                             gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                            >  1:945933/1‑46 (MQ=38)
                                                                                                                                                 aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCg                                                                               >  1:946040/1‑55 (MQ=11)
                                                                                                                                                  |                                                                                                                                  
ATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCG  >  U00096/1708605‑1708881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑