Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	2,947,467	0	A	C	27.7%	135.5 / 48.2	65	intergenic (+4/‑30)	metZ/metW	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (14/33);  new base C (4/14);  total (18/47)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT  >  U00096/2947320‑2947575                                                                                                                                                    |                                                                                                               aaTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaa                                                                                                               <  2:2265428/149‑1 (MQ=255)  aTCCGTTATCGGGGTATAAAAAAGGCTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                                                              <  2:2265432/149‑1 (MQ=255)  aTCCGTGATCGGGGTAAAAAAACGGTTGCATGAAAACGCGAGCGGAGTATAGGGCGCATCCACGGACGCGGGGGGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                              <  1:2265430/149‑1 (MQ=255)  aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                              <  1:2265431/149‑1 (MQ=255)   tCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaaa                                                                                                             <  1:2265433/149‑1 (MQ=255)     cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAtt                                                                                                           <  1:2265435/149‑1 (MQ=255)        gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                                        <  1:2265438/149‑1 (MQ=255)         aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgatg                                                                                                      <  1:2265439/149‑1 (MQ=255)          tCGGGTTAAAAATCCGGTGGCATGAAAACGCGATCGTAGTATAGTTCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgatga                                                                                                     <  2:2265475/149‑1 (MQ=14)          tCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatg                                                                                                      <  2:2265441/149‑1 (MQ=255)           cGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGGGCGCATCCACGGTCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatga                                                                                                     <  2:2265442/149‑1 (MQ=255)            tggttAAAACACAGGTGGCATGAAAACTCTAGCGTAGTATAGTTCGCATACACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAg                                                                                                   <  2:2265529/145‑1 (MQ=14)            tgcgTATAATAAAGGTGGCATGAAATCGCTAGCGGTGAATAGTGGGAATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAg                                                                                                   <  2:2265549/146‑1 (MQ=14)             gggTATAAAAAAGGTTGAATGAAAACTCGAGCGTAGTATAGTTCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑t                                                                                                 <  2:2265446/149‑1 (MQ=17)             gggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑t                                                                                                 >  2:2265533/1‑149 (MQ=255)               gTAAAAAAAAGGTTGCCTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTTGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatga                                                                                                     <  1:2265449/145‑1 (MQ=255)               gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑a                                                                                               <  1:2265448/149‑1 (MQ=255)                tAAAAACAACGTTGAATGAAAACGCGAGCGGAGTAGAGTGCGCCTCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaa                                                                                             <  2:2265451/149‑1 (MQ=17)                tAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑aa                                                                                              <  2:2265450/149‑1 (MQ=255)                 aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑a                                                                                               <  1:2265453/147‑1 (MQ=255)                 aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaa                                                                                            <  2:2265454/149‑2 (MQ=255)                  aaaaaacGGTTCCATCAAAACGCCCGCGCAGTATCGTGCCCATCCACGGCCGCGGGGGGCAGCAGCCTGGTCGCCCGTCGGGCCCAAACCCCGAAGGTCGCCGGTCCAAACCCGGCCCCCGCAACCAATTAAAATTTCATCACG‑T‑aacc                                                                                            <  2:2265455/149‑3 (MQ=255)                    aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCa                                                                                          <  1:2265458/149‑1 (MQ=255)                    aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCACCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCa                                                                                          >  2:2265500/1‑149 (MQ=255)                      aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGt                                                                                        <  2:2265463/149‑1 (MQ=255)                      aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGt                                                                                        <  2:2265464/149‑1 (MQ=255)                      aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGt                                                                                        <  2:2265461/149‑1 (MQ=255)                      aaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCAGCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGt                                                                                        <  2:2265460/149‑1 (MQ=255)                       aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTa                                                                                       <  2:2265465/149‑1 (MQ=255)                        agtttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTAc                                                                                      <  1:2265466/146‑1 (MQ=255)                        aGGCTGCACGAAAACGCGAGCGGAGTAGAGTGCGCATCCTCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTAc                                                                                      <  2:2265468/149‑1 (MQ=14)                          gTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACgg                                                                                    <  2:2265471/149‑1 (MQ=255)                           ttGCATGAAAACGCTAGCGGAGTATATTTCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTACGGt                                                                                   <  2:2265472/149‑1 (MQ=14)                           ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGt                                                                                   <  1:2265474/149‑1 (MQ=255)                           ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGt                                                                                   <  1:2265473/149‑1 (MQ=255)                           ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATGAAATTTTGATGATG‑T‑AAAGCAGTACGGt                                                                                   >  1:2265472/1‑149 (MQ=255)                             gCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGa                                                                                 <  1:2265476/149‑1 (MQ=255)                              tgatgaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAc                                                                                <  2:2265486/144‑1 (MQ=255)                              cATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAc                                                                                <  1:2265478/149‑1 (MQ=255)                               aTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAc                                                                                <  1:2265482/148‑1 (MQ=255)                                tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGAcgc                                                                              <  1:2265483/149‑1 (MQ=255)                                  aaaaCTCGTTCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAA‑CAAATCAAATTTGATGAAG‑T‑aaaag                                                                                           <  2:2265498/133‑3 (MQ=14)                                  aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑TAAAAGCAGTACGGTGACGCgg                                                                            <  2:2265484/149‑1 (MQ=255)                                  aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaagc                                                                                          >  2:2265485/1‑131 (MQ=255)                                  aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaagc                                                                                          <  1:2265485/134‑4 (MQ=255)                                   aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAAGTTGAGGAAGTT‑AAAGc                                                                                           >  1:2265498/1‑133 (MQ=14)                                    catccgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCgggg                                                                          <  1:2265494/145‑1 (MQ=255)                                    aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCgggg                                                                          <  1:2265487/149‑1 (MQ=255)                                     aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGt                                                                         <  2:2265490/149‑1 (MQ=255)                                     aCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGt                                                                         <  1:2265489/149‑1 (MQ=255)                                      cgcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                >  1:2265488/1‑111 (MQ=255)                                       gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGt                                                                         <  1:2265492/147‑1 (MQ=255)                                        cgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGa                                                                      <  1:2265493/149‑1 (MQ=255)                                          aGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGagc                                                                    <  2:2265496/149‑1 (MQ=255)                                           gCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                >  1:2265495/1‑106 (MQ=255)                                              gAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTg                                                                        <  1:2265500/141‑1 (MQ=255)                                               aGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCGACCAA‑AAAAATTATCTGCAG‑TAAAAGCAGTACGGTGCCGCGGGGTGGAGCAGCCt                                                               >  1:2265601/1‑149 (MQ=11)                                                 tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAATTTTAATAAGG‑A‑AAAGCAGTACGGTGa                                                                                 >  2:2265536/1‑129 (MQ=14)                                                  ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                >  1:2265587/1‑99 (MQ=255)                                                    aGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                                                              >  1:2265537/1‑99 (MQ=255)                                                    aGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaag                                                                                           <  2:2265538/115‑3 (MQ=255)                                                    aGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAG‑T‑aaaag                                                                                           >  1:2265538/1‑113 (MQ=255)                                                     gTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                                                              >  2:2265550/1‑98 (MQ=255)                                                      tGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                                >  1:2265599/1‑95 (MQ=255)                                                          cATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTAATAAAG‑A‑AAAGCAGTACGGTGACGCgg                                                                            >  2:2265527/1‑125 (MQ=255)                                                           aTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTATTAAGG‑T‑AAAGCAGTACGGTGACGGGGGGGGGTGCTGGATGGTAGCacgtgg                                                   >  2:2265534/1‑147 (MQ=11)                                                                             gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTAc                                                                                      <  1:2265507/96‑1 (MQ=255)                                                                                      ggTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                              >  1:2265555/1‑65 (MQ=33)                                                                                                    cTCATAACCCGAAGGTCGTCGGTTCAAATCCTGCCCCCGCAACCAATTAAAATTtgatga                                                                                                     <  1:2265508/60‑1 (MQ=255)                                                                                                                                            aaCCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                    >  2:2265573/1‑67 (MQ=255)                                                                                                                                             aCCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCaa   >  1:2265614/1‑115 (MQ=255)                                                                                                                                                aaTTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt  >  1:2265570/1‑113 (MQ=40)                                                                                                                                                aaTTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt  >  1:2265562/1‑113 (MQ=40)                                                                                                                                                 aTTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGcc              >  2:2265572/1‑100 (MQ=40)                                                                                                                                                 aTTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt  >  1:2265632/1‑112 (MQ=39)                                                                                                                                                  ttAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt  >  1:2265564/1‑111 (MQ=38)                                                                                                                                                  ttAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt  >  1:2265560/1‑111 (MQ=38)                                                                                                                                                    |                                                                                                               AATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAG‑T‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT  >  U00096/2947320‑2947575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑