Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,552,1910CA21.2% 348.4 / 16.0 146intergenic (+200/+207)fdnI/yddMformate dehydrogenase N subunit gamma/DNA‑binding transcriptional regulator YddM
Reads supporting (aligned to +/- strand):  ref base C (40/75);  new base A (21/10);  total (61/85)
Fisher's exact test for biased strand distribution p-value = 1.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 147 total aligned reads displayed.

TGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGC  >  U00096/1552046‑1552338
                                                                                                                                                 |                                                                                                                                                   
tGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaaa                                                                                                                                                  <  2:1162606/149‑1 (MQ=33)
 gTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAt                                                                                                                                                 <  2:1162608/149‑1 (MQ=34)
 gTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAAt                                                                                                                                                 <  1:1162609/149‑1 (MQ=34)
  tAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATg                                                                                                                                                <  1:2265921/149‑1 (MQ=34)
  tAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATg                                                                                                                                                <  1:1162610/149‑1 (MQ=34)
   aGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATgg                                                                                                                                               <  1:1162611/149‑1 (MQ=34)
    gACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgc                                                                                                                                              <  1:1162613/149‑1 (MQ=34)
    gACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgc                                                                                                                                              <  2:1162614/149‑1 (MQ=34)
         cGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGt                                                                                                                                         <  2:1162617/149‑1 (MQ=35)
         cGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGt                                                                                                                                         <  2:1162616/149‑1 (MQ=35)
         cGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGt                                                                                                                                         >  2:1162754/1‑149 (MQ=18)
           tAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaa                                                                                                                                       <  2:1162620/149‑1 (MQ=38)
           tAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaa                                                                                                                                       <  2:1162619/149‑1 (MQ=38)
           tAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTaa                                                                                                                                       >  2:1162751/1‑149 (MQ=17)
            aGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaaa                                                                                                                                      <  1:1162621/149‑1 (MQ=38)
             ggCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaaaa                                                                                                                                     >  2:1162659/1‑149 (MQ=39)
               cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGTCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAgg                                                                                                                                   <  2:1162624/149‑1 (MQ=255)
               cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAgg                                                                                                                                   <  1:1162625/149‑1 (MQ=40)
                 ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCg                                                                                                                                 >  2:1162675/1‑149 (MQ=255)
                 ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCg                                                                                                                                 <  2:1162626/149‑1 (MQ=255)
                   gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTc                                                                                                                               <  1:1162627/149‑1 (MQ=255)
                      cATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAtt                                                                                                                            <  1:1162630/149‑1 (MQ=255)
                      cATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAtt                                                                                                                            <  2:1162629/149‑1 (MQ=255)
                       aTGCCTGATGCTGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                                                                                                                           <  2:1162634/149‑1 (MQ=255)
                       aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                                                                                                                           <  2:1162632/149‑1 (MQ=255)
                       aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTACAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                                                                                                                           <  1:1162633/149‑1 (MQ=255)
                          ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCAGATAAGCGTAGCTCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATATTCa                                                                                                                        >  2:1568708/1‑149 (MQ=255)
                              aTGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt                                                                                                                    <  2:1162636/149‑1 (MQ=255)
                                  ggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGATGTAAAAGGCGTCATTTTCATATTGTAg                                                                                                                >  1:1162686/1‑149 (MQ=255)
                                     gTGAACGCCTTATCCGGCATGAAAACCCTTCAATTCCAATAGATTGCAGTGTACGTGTAGGCCTTATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCTTCAGTTTCAAATGGCGCTGTATAAGTCGTCATTTTCATATTGTAGACa                                                                                                             <  2:1162638/149‑1 (MQ=17)
                                       gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt                                                                                                                    <  1:1162642/140‑1 (MQ=255)
                                       gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAc                                                                                                              <  1:1162641/146‑1 (MQ=255)
                                       gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAAc                                                                                                           <  2:1162640/149‑1 (MQ=255)
                                       gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAAc                                                                                                           <  2:1162643/149‑1 (MQ=255)
                                        aaCGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACg                                                                                                          <  1:1162645/149‑1 (MQ=255)
                                          cGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTa                                                                                                        <  1:1162646/149‑1 (MQ=255)
                                                aTCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCttt                                                                                                  >  1:1162681/1‑149 (MQ=255)
                                                 tCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACa                                                                                                             <  1:1162649/137‑1 (MQ=255)
                                                 tCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTg                                                                                                 <  1:1162650/149‑1 (MQ=255)
                                                  ccGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCATGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGt                                                                                                <  2:1162651/149‑1 (MQ=255)
                                                   cGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGTACGTGTAGTCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTTTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGtt                                                                                               <  2:1162653/149‑1 (MQ=255)
                                                   cGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGTCATTTTAATATTGAAGACAACGAAGGCTTTGta                                                                                               >  2:1162813/1‑148 (MQ=17)
                                                    ggCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCAGCAGTTTCAAATGGCGCTGGAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGtt                                                                                               >  1:1162710/1‑148 (MQ=255)
                                                     gCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCa                                                                                             <  2:1162655/149‑1 (MQ=255)
                                                        tgcaaaCCCTGCACATACATTAGATTGCATCGTACGAGTAGTCCTGATAAGGGGACCTCATCAGGAAATGTTTCGTTTTGCATAAGTTGCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGc                                                                                          <  1:1162688/146‑1 (MQ=12)
                                                            aaCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGa                                                                                      <  1:1162657/149‑1 (MQ=255)
                                                            aaCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGa                                                                                      <  1:1162658/149‑1 (MQ=255)
                                                              cccTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATg                                                                                    <  1:1162659/149‑1 (MQ=255)
                                                               ccTTCAAATCCAATACATAGCAGTGCACGTGTCGGCCTTATAAGCTTAGCGCATCAGGCTATGTTGCGATAGTCATCAGCTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgc                                                                                   <  2:1162662/149‑1 (MQ=21)
                                                                 ttCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcgg                                                                                 <  2:1162663/149‑1 (MQ=255)
                                                                     attCCAATAGATTGCAGTGAACGTGTATGCCTGATTAGCGTAGAGCATCAGGCAATTTTGAGATTTTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTg                                                                             <  2:1162666/147‑1 (MQ=21)
                                                                       tCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGaa                                                                           <  2:1162667/149‑1 (MQ=255)
                                                                        ccAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAAc                                                                          <  1:1162668/149‑1 (MQ=255)
                                                                          aaTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATAAGGCGGCATTTTCATATTGTAGACACCGTAGGCTTTGTTCATGCCGGATGCGGCGTGat                                                                           >  2:1162842/1‑145 (MQ=255)
                                                                                ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                  <  2:1209036/149‑1 (MQ=255)
                                                                                 tGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGATTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc                                                                 <  1:1162669/149‑1 (MQ=255)
                                                                                     gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTCTCCGGCa                                                             >  2:1162829/1‑149 (MQ=255)
                                                                                        aaCGTGTAGGCCTGATAAGCGTAGCGCATGAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGa                                                          <  2:1162671/149‑1 (MQ=255)
                                                                                          cGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaaa                                                        <  2:1162673/149‑1 (MQ=255)
                                                                                          cGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaaa                                                        <  2:1162672/149‑1 (MQ=255)
                                                                                          cGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGAGTCATTTTAAAATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGTCGTTAAAGACTTATACGGCATGaaa                                                        >  2:1162869/1‑149 (MQ=21)
                                                                                             gtAGGCCTGATAAGCGTAGCGCATCATGCAATGTTGCGTTTGTCATCAGTTTAAAATGGAGCTGTAAAAGGAGACATTTTAATATTGAAGACAACGTAGGAGTTGATCATACCGGATGCGGCGTGAGAGCAGTACACGGCATGAAAAca                                                     >  2:1162833/1‑148 (MQ=18)
                                                                                             gtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATAAGGCGGCATTTTATTATTGTAGGCAACGTAGGCTTTGTTCATGACGGATGAGGCGTGGACGCGTTATCCGGCATGAAAAcc                                                     >  2:1162835/1‑149 (MQ=21)
                                                                                                  ccTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTg                                                                             >  1:3538481/1‑120 (MQ=255)
                                                                                                  ccTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa                                                <  2:1162676/149‑1 (MQ=255)
                                                                                                   cTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCt                                                                                                                                           >  1:1162822/1‑57 (MQ=255)
                                                                                                      aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATc                                            <  2:1162680/149‑1 (MQ=255)
                                                                                                      aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATc                                            >  1:1162679/1‑149 (MQ=255)
                                                                                                       tAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAtttt                                                                                                                          >  1:1162895/1‑70 (MQ=255)
                                                                                                       tAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGAGTCATTTTCATATTGTAGACAAAGTCGGCTTTGTTCATGCCGGATGCGGCGTGAAAGACATATACGGCATGAAAACCCTTGAAAtac                                           >  2:1162844/1‑147 (MQ=18)
                                                                                                        aaGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCa                                          <  2:1162681/149‑1 (MQ=255)
                                                                                                        aaGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTa                                                                                                                                        >  1:1162832/1‑55 (MQ=255)
                                                                                                          gCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                        <  2:1162684/149‑1 (MQ=255)
                                                                                                              aGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTATGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAt                                    <  2:1162686/149‑1 (MQ=255)
                                                                                                                 gcATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGAGGCATTTTAATATTg                                                                                                                   >  1:1162764/1‑67 (MQ=18)
                                                                                                                   aTCAGGCAATGTTTCGTTTTTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAg                               <  2:1162689/149‑1 (MQ=255)
                                                                                                                    tCAGGCAATGTTGCTTTATGCATCAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGt                              <  2:1162700/149‑1 (MQ=11)
                                                                                                                    tCAGGCAATGTTGCGTTTTCCATCAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGt                              <  2:1162690/149‑1 (MQ=14)
                                                                                                                    tCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTAATATTg                                                                                                                   >  1:1162820/1‑64 (MQ=21)
                                                                                                                          aaTGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGGGGCATTTTATTATTGTATCCAACGTAGGCTATGTTCCTGCCGGATGCGGCGTGAACGACTGATGCGGCAAGAACACCCTTCAAATACAATAGATGGCAGTGCACgt                        >  2:1162880/1‑149 (MQ=17)
                                                                                                                          aaTGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTa                                                                                                                                        >  1:1162862/1‑37 (MQ=255)
                                                                                                                               tGCGTTTGTCATCAGTTTCAAATGGCGCTTTAAAAGGCGTCAtttt                                                                                                                          >  1:1162796/1‑46 (MQ=255)
                                                                                                                               tGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCGTATCCGGCATGAACACCCGTCAAATCCAATAGATTGCAGTGAACGTGTAgg                   >  2:1162849/1‑149 (MQ=255)
                                                                                                                                 cGTTTGTCATCAGTTTCAAATGGCGCGGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGcc                 <  1:2704666/149‑1 (MQ=255)
                                                                                                                                  gTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCt                >  2:1162855/1‑149 (MQ=255)
                                                                                                                                  gTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCt                >  1:1162848/1‑149 (MQ=255)
                                                                                                                                   tttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg               >  2:1162878/1‑149 (MQ=255)
                                                                                                                                   tgtctcataaGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg               <  2:1162697/145‑1 (MQ=255)
                                                                                                                                    ttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGa              >  1:1162894/1‑149 (MQ=255)
                                                                                                                                    ttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGa              >  2:1162850/1‑149 (MQ=255)
                                                                                                                                        catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGt                                                                                                >  1:1162838/1‑63 (MQ=255)
                                                                                                                                        catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAg          >  2:1162804/1‑149 (MQ=40)
                                                                                                                                         atcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                             <  1:1162696/97‑1 (MQ=255)
                                                                                                                                          tcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCg        >  2:1162762/1‑149 (MQ=38)
                                                                                                                                            aGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTa      >  2:1162800/1‑149 (MQ=37)
                                                                                                                                             gTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg     >  1:1162834/1‑149 (MQ=35)
                                                                                                                                             gTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg     >  2:1162787/1‑149 (MQ=35)
                                                                                                                                              tttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgc    >  1:1162755/1‑149 (MQ=35)
                                                                                                                                               ttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcg   >  2:1162763/1‑149 (MQ=34)
                                                                                                                                               ttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg     >  1:1162775/1‑147 (MQ=34)
                                                                                                                                                 |                                                                                                                                                   
TGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGC  >  U00096/1552046‑1552338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑