Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	4,255,502	T→C	Q322R (CAG→CGG)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	4,255,502	0	T	C	100.0%	181.1 / NA	48	Q322R (CAG→CGG)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/17);  total (31/17)

AGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAG  >  U00096/4255358‑4255645                                                                                                                                                 |                                                                                                                                                agaTACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGt                                                                                                                                              >  1:946822/1‑148 (MQ=255)   aTACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGa                                                                                                                                            >  1:946825/1‑148 (MQ=255)          cGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCa                                                                                                                                     >  1:946836/1‑148 (MQ=255)          cGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCa                                                                                                                                     >  2:946796/1‑148 (MQ=255)               aaCGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGCTCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGAGATACAGCACGTaa                                                                                                                                >  2:946826/1‑148 (MQ=255)                   gTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGaaa                                                                                                                            <  1:946798/148‑1 (MQ=255)                    tGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAag                                                                                                                           >  2:946813/1‑148 (MQ=255)                    tGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAag                                                                                                                           >  2:946815/1‑148 (MQ=255)                     ggAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAg                                                                                                                               >  1:946829/1‑143 (MQ=255)                           aaGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGcagcag                                                                                                                     <  1:946799/147‑1 (MQ=255)                                 aTTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTcc                                                                                                              >  2:946833/1‑148 (MQ=255)                                 aTTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTcc                                                                                                              >  2:946811/1‑148 (MQ=255)                                   tGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAt                                                                                                            >  1:946837/1‑148 (MQ=255)                                      cTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCAtgtg                                                                                                         <  2:946800/148‑1 (MQ=255)                                        ttAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGAc                                                                                                       <  2:946801/148‑1 (MQ=255)                                         tAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACt                                                                                                      <  1:946802/148‑1 (MQ=255)                                          aaaCGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCCGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTg                                                                                                     >  2:946845/1‑148 (MQ=255)                                            aCGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCg                                                                                                   >  2:946821/1‑148 (MQ=255)                                             cGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCgg                                                                                                  <  1:946803/148‑1 (MQ=255)                                                   gCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCaa                                                                                            >  2:946824/1‑148 (MQ=255)                                                     gAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAgg                                                                                          <  2:946804/148‑1 (MQ=255)                                                      aaTAAAGAACGCCCCTAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGAGACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGc                                                                                         >  2:946832/1‑148 (MQ=255)                                                        tAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGcac                                                                                       >  2:946846/1‑148 (MQ=255)                                                          aaGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACat                                                                                     <  2:946805/148‑1 (MQ=255)                                                           aGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCATCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACata                                                                                    >  2:946817/1‑148 (MQ=255)                                                            gAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACatat                                                                                   >  1:946841/1‑148 (MQ=255)                                                              aCGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATAc                                                                                 <  2:946806/148‑1 (MQ=255)                                                                    ccAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTc                                                                           <  2:946807/148‑1 (MQ=255)                                                                    ccAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTc                                                                           >  2:946816/1‑148 (MQ=255)                                                                     cAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCa                                                                          >  1:946842/1‑148 (MQ=255)                                                                       gACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATg                                                                        >  2:946830/1‑148 (MQ=255)                                                                               aaaaTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACcgtcgt                                                                <  1:946808/148‑1 (MQ=255)                                                                               aaaaTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACcgtcgt                                                                >  1:946852/1‑148 (MQ=255)                                                                               aaaaTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACcgtcgt                                                                >  2:946854/1‑148 (MQ=255)                                                                                  aTCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTggg                                                             >  1:946848/1‑148 (MQ=255)                                                                                   tCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCACGGTGATACAGAACGTAAGAAAGCAGCAGGTACTCCATGTTACTGAGGTGGCAAGGCACATATACCAGCTCATTACCGTCGTggtc                                                            >  1:946827/1‑146 (MQ=255)                                                                                                ccAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAAcg                                               <  2:946809/148‑1 (MQ=255)                                                                                                   gAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGa                                                                       <  1:946810/121‑1 (MQ=255)                                                                                                    aaaTTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCt                                           >  2:946843/1‑148 (MQ=255)                                                                                                     aaTTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTc                                          <  1:946811/148‑1 (MQ=255)                                                                                                            gTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTa                                   <  2:946812/148‑1 (MQ=255)                                                                                                             ttGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTAt                                  >  2:946828/1‑148 (MQ=255)                                                                                                                   ccGGCGGCGATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGt                            <  1:946813/148‑1 (MQ=255)                                                                                                                         gcgATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATg                       >  1:946814/1‑147 (MQ=255)                                                                                                                          cgATATGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGcc                     >  2:946835/1‑148 (MQ=255)                                                                                                                              atGCGGCGGCACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTgg                 >  2:946855/1‑148 (MQ=255)                                                                                                                                   gcggcACCAGCCCCCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAg            >  2:946831/1‑148 (MQ=255)                                                                                                                                             ccccCGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAg  <  1:946815/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                AGATACTCCCGGAAAACGGTGGAATAAAGTTTATTGCCTTTAAACGTACGGCGAATAAAGAACGCCCCCAGACGGCGGAAAATCGGCCCGGCAGGCCAGAAATTCAGGTTGATCCCGGCGGCGATATGCGGCGGCACCAGCCCCTGGTGATACAGCACGTAAGAAAGCAGCAGGTAGTCCATGTGACTGCGGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAG  >  U00096/4255358‑4255645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑