BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	1,299,499	Δ1,199 bp		insH21	insH21

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	1299499–1300697	1300697	1–1199	153 [0]	[0] 153	insH21	insH21

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 1299498	0 (0.000)	145 (1.450)	113/284	0.0	100%	intergenic (+253/‑69)	ychE/insH21	MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
*	?	U00096	1300698 =	0 (0.000)	145 (1.450)	113/284	0.0	100%	intergenic (+151/‑484)	insH21/oppA	IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein

Only 100 of 153 total aligned reads displayed.

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAA  >  U00096/1300550‑1300846

ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                       <  1:443742‑M2/1‑1 (MQ=255)

 tctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaat                                                                                                                                                      >  2:444136‑M2/148‑149 (MQ=255)

    caaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                                    >  1:443743‑M2/145‑148 (MQ=255)

     aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                  >  2:444069‑M2/144‑149 (MQ=255)

     aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                  >  1:444057‑M2/144‑149 (MQ=255)

      aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggctttctgaagaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                                 >  1:444065‑M2/143‑149 (MQ=255)

      aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                                 <  1:443745‑M2/7‑1 (MQ=255)

       attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                                >  2:444078‑M2/142‑149 (MQ=255)

         tacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCa                                                                                                                                              <  2:443746‑M2/10‑1 (MQ=255)

          acagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                             >  2:444068‑M2/139‑149 (MQ=255)

            agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                           >  2:444120‑M2/137‑149 (MQ=255)

            agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                           >  2:444119‑M2/137‑149 (MQ=255)

              ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑t                                                                                                                                        <  2:443748‑M2/15‑1 (MQ=255)

                  ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTaaa                                                                                                                                    <  1:443749‑M2/19‑1 (MQ=255)

                   tataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTaaa                                                                                                                                    <  1:443751‑M2/19‑1 (MQ=255)

                   tataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAat                                                                                                                                   >  2:444083‑M2/130‑149 (MQ=255)

                   tataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAat                                                                                                                                   >  1:444088‑M2/130‑149 (MQ=255)

                     taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAatt                                                                                                                                  >  1:444085‑M2/128‑148 (MQ=255)

                      aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAattat                                                                                                                                <  1:443752‑M2/23‑1 (MQ=255)

                       aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAattatt                                                                                                                               <  2:443754‑M2/24‑1 (MQ=255)

                           tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGc                                                                                                                           >  2:444101‑M2/122‑149 (MQ=255)

                           tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGc                                                                                                                           <  2:443756‑M2/28‑1 (MQ=255)

                           tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGc                                                                                                                           <  1:443757‑M2/28‑1 (MQ=255)

                            ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgataatattaattccacagcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTaaa                                                                                                                                    <  1:443758‑M2/19‑1 (MQ=255)

                                 taactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTc                                                                                                                      >  1:444141‑M2/116‑148 (MQ=255)

                                 taactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagagaaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCa                                                                                                                     >  1:444072‑M2/116‑149 (MQ=255)

                                   actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCact                                                                                                                   >  1:444073‑M2/114‑149 (MQ=255)

                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCactact                                                                                                                >  2:444096‑M2/111‑149 (MQ=255)

                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCactact                                                                                                                <  1:443761‑M2/39‑1 (MQ=255)

                                      gcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCactact                                                                                                                >  2:444089‑M2/111‑149 (MQ=255)

                                       cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTg                                                                                                               >  1:444112‑M2/110‑149 (MQ=255)

                                        ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTgg                                                                                                              >  1:444103‑M2/109‑149 (MQ=255)

                                         tctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaatatttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCAGTACTGGa                                                                                                             >  2:444144‑M2/108‑149 (MQ=255)

                                          ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGaa                                                                                                            <  2:443762‑M2/43‑1 (MQ=255)

                                            cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGa                                                                                                             <  2:443763‑M2/42‑1 (MQ=255)

                                                  catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                    <  1:443764‑M2/51‑1 (MQ=255)

                                                   atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                                   >  1:444107‑M2/98‑149 (MQ=255)

                                                    tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAg                                                                                                  >  2:444132‑M2/97‑149 (MQ=255)

                                                     catgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                 <  2:443766‑M2/54‑1 (MQ=255)

                                                      atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                                <  2:443767‑M2/55‑1 (MQ=255)

                                                             tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                         >  1:444131‑M2/88‑149 (MQ=255)

                                                               tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                       <  2:443770‑M2/64‑1 (MQ=255)

                                                                cgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACa                                                                                      <  2:443771‑M2/65‑1 (MQ=255)

                                                                  ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                    <  2:443773‑M2/67‑1 (MQ=255)

                                                                    acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                                  >  2:444166‑M2/81‑149 (MQ=255)

                                                                        cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                              <  1:444078‑M2/73‑1 (MQ=255)

                                                                         ataatcctgggcttgctgaagaataattgaaatgatattattaattcgactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAGTTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                             >  2:444148‑M2/76‑149 (MQ=255)

                                                                          taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCa                                                                            >  1:444139‑M2/75‑149 (MQ=255)

                                                                           aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCaa                                                                           <  1:444099‑M2/76‑1 (MQ=255)

                                                                            atcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAt                                                                          <  1:444061‑M2/77‑1 (MQ=255)

                                                                                tgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                      >  2:444133‑M2/69‑149 (MQ=255)

                                                                                 gggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAGCTCTa                                                                     <  2:444075‑M2/82‑1 (MQ=255)

                                                                                   gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                                   <  1:444070‑M2/84‑1 (MQ=255)

                                                                                    cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                                  <  2:444060‑M2/85‑1 (MQ=255)

                                                                                    cttgctgaagaataattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                         <  1:444062‑M2/62‑1 (MQ=255)

                                                                                     ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttg                                                                 >  1:444113‑M2/64‑149 (MQ=255)

                                                                                       gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                               >  2:444190‑M2/62‑149 (MQ=255)

                                                                                         tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattg                                                             <  2:444092‑M2/90‑1 (MQ=255)

                                                                                          gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattga                                                            <  1:444064‑M2/91‑1 (MQ=255)

                                                                                            agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                          >  2:444165‑M2/57‑149 (MQ=255)

                                                                                             gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                         >  1:444121‑M2/56‑149 (MQ=255)

                                                                                              aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAACGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                        <  2:444077‑M2/95‑1 (MQ=255)

                                                                                                  attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAg                                                    >  1:444118‑M2/51‑149 (MQ=255)

                                                                                                  attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAg                                                    <  2:444091‑M2/99‑1 (MQ=255)

                                                                                                   ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                   <  1:444083‑M2/100‑1 (MQ=255)

                                                                                                     gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtg                                                 <  1:444079‑M2/102‑1 (MQ=255)

                                                                                                        atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                              <  1:444089‑M2/105‑1 (MQ=255)

                                                                                                        atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATATCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                              >  2:444184‑M2/45‑149 (MQ=255)

                                                                                                         ttatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                             <  2:444065‑M2/106‑1 (MQ=255)

                                                                                                         tgatattattaattccactgcctttggtagagaaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                             <  2:444072‑M2/106‑1 (MQ=255)

                                                                                                          gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                            <  2:444073‑M2/107‑1 (MQ=255)

                                                                                                           atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                           >  1:444164‑M2/42‑149 (MQ=255)

                                                                                                           atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                           >  2:444127‑M2/42‑149 (MQ=255)

                                                                                                             attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                         <  1:444067‑M2/110‑1 (MQ=255)

                                                                                                             attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                         <  1:444096‑M2/110‑1 (MQ=255)

                                                                                                             attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                         <  2:444081‑M2/110‑1 (MQ=255)

                                                                                                             attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTa                                          >  1:444102‑M2/40‑148 (MQ=255)

                                                                                                               tattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAgg                                       >  2:444158‑M2/38‑149 (MQ=255)

                                                                                                                attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                      >  2:444137‑M2/37‑149 (MQ=255)

                                                                                                                attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                      >  2:444173‑M2/37‑149 (MQ=255)

                                                                                                                 ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                        >  1:444147‑M2/36‑146 (MQ=255)

                                                                                                                     ttccactgcctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                                 >  1:444135‑M2/32‑149 (MQ=255)

                                                                                                                             cctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAATGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                         >  2:444157‑M2/24‑149 (MQ=255)

                                                                                                                              ctttggtagaggaaagtgctaaATAATACTCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                        <  1:444069‑M2/127‑1 (MQ=255)

                                                                                                                              ctttggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                        <  2:444098‑M2/127‑1 (MQ=255)

                                                                                                                                 tggtagaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg                     <  2:444085‑M2/130‑1 (MQ=255)

                                                                                                                                  ggtagaggaaagtgctaaATAATAATCAATTGTTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg                     >  1:444143‑M2/19‑149 (MQ=255)

                                                                                                                                     agaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc                 <  2:444088‑M2/134‑1 (MQ=255)

                                                                                                                                      gaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt                >  1:444150‑M2/15‑149 (MQ=255)

                                                                                                                                      gaggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt                <  1:444095‑M2/135‑1 (MQ=255)

                                                                                                                                        ggaaagtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa              >  2:444209‑M2/13‑149 (MQ=255)

                                                                                                                                             gtgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg         <  2:444086‑M2/142‑1 (MQ=255)

                                                                                                                                              tgctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt        <  1:444100‑M2/143‑1 (MQ=255)

                                                                                                                                               gctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTc       <  2:444057‑M2/144‑1 (MQ=255)

                                                                                                                                               gctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTc       >  1:444058‑M2/6‑149 (MQ=255)

                                                                                                                                                ctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa      >  1:444171‑M2/5‑149 (MQ=255)

                                                                                                                                                ctaaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCa      <  1:957061‑M2/145‑1 (MQ=255)

                                                                                                                                                  aaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAt    <  2:444102‑M2/147‑1 (MQ=255)

                                                                                                                                                  aaATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAAt    >  2:444177‑M2/3‑149 (MQ=255)

                                                                                                                                                    ataataatCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATaa  >  1:444197/1‑149 (MQ=255)

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTG‑TTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAA  >  U00096/1300550‑1300846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`