Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4350TG23.3% 213.6 / 13.6 87intergenic (+76/‑83)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (8/58);  new base G (20/0);  total (29/58)
Fisher's exact test for biased strand distribution p-value = 1.70e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  U00096/422292‑422583
                                                                                                                                               |                                                                                                                                                    
ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGccccc                                                                                                                                                 >  1:143825/1‑149 (MQ=255)
 tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCa                                                                                                                                                >  2:143824/1‑149 (MQ=255)
  gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                               <  1:143754/149‑1 (MQ=255)
    tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                             <  1:143755/149‑1 (MQ=255)
       gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATc                                                                                                                                          >  2:143798/1‑149 (MQ=255)
        cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                         <  1:143756/149‑1 (MQ=255)
         tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTATTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCGCCCCCCAAGATCCt                                                                                                                                        >  2:143815/1‑149 (MQ=255)
         tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                        <  2:143757/149‑1 (MQ=255)
            cgATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                                                      <  1:143758/147‑1 (MQ=255)
               ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                                  <  1:143760/149‑1 (MQ=255)
               ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                                   <  1:143759/148‑1 (MQ=255)
                   cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                              <  1:143761/149‑1 (MQ=255)
                    tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGCCGTCCTCCGCCGCCAAATATCCTTCAGAtgatga                                                                                                                             >  2:143822/1‑149 (MQ=255)
                    tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                             <  1:143763/149‑1 (MQ=255)
                    tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                             <  1:143762/149‑1 (MQ=255)
                     ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                            <  1:143764/149‑1 (MQ=255)
                        tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                         <  2:143765/149‑1 (MQ=255)
                         gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                        <  1:143766/149‑1 (MQ=255)
                          tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                       <  1:143768/149‑1 (MQ=255)
                          tttAAACGCCGCCACGAGCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                       <  2:143767/149‑1 (MQ=255)
                           ttAAACGCCGCCACGATCGTCAGCTGGCTTAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                       <  1:1128986/148‑1 (MQ=255)
                             aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTCTTCCGAAAGTTATCCGCCCCCGTCCGACGCCCCCACATATCCATCAGATGCGGAGTGTGCCTg                                                                                                                    >  1:143832/1‑149 (MQ=255)
                              aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGCCGCCGCCCCCAAATATGCTTCAGATGATGAGTGAtggtgg                                                                                                                   >  2:143811/1‑144 (MQ=255)
                              aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                                   <  1:143769/149‑1 (MQ=255)
                                 gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATACGCCCCCTTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                                <  2:143770/149‑1 (MQ=255)
                                 gccgccACGATCGTCAGCTGGCTGAAAACCAGTAAGCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                                <  2:1327824/149‑1 (MQ=255)
                                   cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                              <  2:143771/149‑1 (MQ=255)
                                     ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                            <  2:143772/149‑1 (MQ=255)
                                     ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                            <  2:143773/149‑1 (MQ=255)
                                      cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGTAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                            <  1:143774/148‑1 (MQ=255)
                                        cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGCCGTCCGCCGCCCCCCAATATACATCGGATGGTGAGTGTTCCGGCATGAGGCGAt                                                                                                         >  2:143803/1‑149 (MQ=255)
                                        cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                         <  2:143775/149‑1 (MQ=255)
                                         gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                        <  1:143776/149‑1 (MQ=255)
                                             gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGGGCTCCGCCCCCAACGAACCTTCAGATGCTGAGGGGGCCTGCATTAGGCTATGGCaa                                                                                                    >  2:143836/1‑149 (MQ=255)
                                             gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                                    <  1:143777/149‑1 (MQ=255)
                                                aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGTCCTCCTCCACCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                                 <  2:143778/149‑1 (MQ=255)
                                                       tgtaaaCCATTAATCCTTTGGTTCTGTTATGCGGTTCATGTTGTAATCCTGATTCGGCTTATCCGTAATTTTTCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                          <  2:143805/146‑1 (MQ=255)
                                                       tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGa                                                                                          <  2:143779/149‑1 (MQ=255)
                                                        gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGGCGGCTGCCCCCAATATCCTGCAGATGAGGAGTGATACTGCAATAGTCTATGGGAAGGAGATCatat                                                                                         >  2:143847/1‑149 (MQ=255)
                                                        gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCGCGGCCCCCAACTAACCGGCAGATGAGGAGTGATACTGGAGTAGGCGATGGAAAGGGGagc                                                                                             >  2:143808/1‑143 (MQ=255)
                                                        gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCGGGCCGCCGCCCCCCAATATCCTGCAGAGGAGGAGTGATGCGGCATGAGGGTATGGCAAGGTGATc                                                                                             >  2:143848/1‑145 (MQ=255)
                                                        gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                         <  2:143780/149‑1 (MQ=255)
                                                           aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATAAACTTAAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                      >  2:143817/1‑149 (MQ=255)
                                                            aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                     <  1:143781/149‑1 (MQ=255)
                                                             ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                    <  1:143782/149‑1 (MQ=255)
                                                             ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                    <  1:143783/149‑1 (MQ=255)
                                                               aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCGTAAGATGATGAGTGGTGCTGCATTAGGCTATGGCAAGGGGATGAGATTTtaata                                                                                  >  2:143804/1‑148 (MQ=255)
                                                                      cTTGTGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                              <  1:143784/146‑1 (MQ=255)
                                                                      cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCACCACCACCCCCAAAAAAACATAAGATGAATAGTGATACAGCATAAAGCTATGGCAACGTGATCAAATATTAATaacacagg                                                                           >  2:143833/1‑145 (MQ=255)
                                                                           gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                      <  2:143785/149‑1 (MQ=255)
                                                                            ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                     <  2:143786/149‑1 (MQ=255)
                                                                             tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATGCGGCATTAGGCTATGGCAAGGTGGTGAGATTTTGATCACAGGGGAATTatg                                                                    >  2:143826/1‑149 (MQ=255)
                                                                               tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                  <  1:143787/149‑1 (MQ=255)
                                                                                gtcaGCCGGATCAGGAGGAACTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                                    <  2:143788/143‑1 (MQ=255)
                                                                                     ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                                <  1:143789/129‑1 (MQ=255)
                                                                                      cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                           <  2:143790/149‑1 (MQ=255)
                                                                                       ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGGCGGGCGCGGCCCCCCAGGATCAGGCAGATGAGGAGTGATGAAGCTGTAGGGTATGGCAAGGGGAGGAGATGTTGATAACAGGGGAATTATGATGTTAAATg                                                          >  2:143827/1‑149 (MQ=255)
                                                                                         ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                        <  1:143791/149‑1 (MQ=255)
                                                                                          tCATGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                       <  2:143792/149‑1 (MQ=255)
                                                                                          tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                       <  2:143793/149‑1 (MQ=255)
                                                                                          tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                       <  1:143794/149‑1 (MQ=255)
                                                                                             ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGTCCGCCGGCGCCAAATATCCGTCAGTTGAGGATTGGTCGGGGATTTGGGTTTGGGCGGGGGGGGGGATTTTAATAACAGGGGAATGATGAGGTTTAATGCAGGGc                                                    >  2:143855/1‑149 (MQ=255)
                                                                                             ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGGTGCTGCATTAGGCTATGGCAAGGTGATGAGATTTTGATCAAAGGGGAATTATGTTGTTTATTTCATGGc                                                    >  2:143834/1‑149 (MQ=255)
                                                                                                ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                                 <  2:143795/149‑1 (MQ=255)
                                                                                                      ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                           <  2:143796/149‑1 (MQ=255)
                                                                                                       cTGATCCGGCTATTCCGAAAGTTCTCCGGCCCCGGGCGGCGCCCCCCCAAAAACTGCAGAGGATGAGGGATGCGGCATTAGGCGAGGGGAAGGGGCGAAGATGTTCGTCACAGGGGATTTTTGATGTTATATGGCTGGCCCCTGCCggg                                          >  2:143821/1‑148 (MQ=255)
                                                                                                        tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCAGCAGCCCCCCAATATCCATCAGAAGATGAGTGATACTGGAGGAGGCGATGGCAAGGTGGTGAGTTTTTAATGAGAGGGGGATTTTGATGTTTAATGCATTGCTCCTGCCGGTg                                         >  2:143838/1‑149 (MQ=255)
                                                                                                              ggCTATTCCGAAAGTTATCCGGCGCCGTCCGGCGCCCCCAAATATCCGTCAGATGATGAGTGAGCCTGCATTAGGCTATGGCGAGGGGTGGAGATTTTGTTGTCCGGGGAATTATGATGTTAAATGAATGGCAGCGGCCGGGGGCCCCa                                   >  2:143837/1‑149 (MQ=255)
                                                                                                              ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                                   <  2:143797/149‑1 (MQ=255)
                                                                                                              ggCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCCAAATCCGTACGAGGGTGAGGGGTCCTGCCGTAGGCTAGGGCAAGGTGAGCAGAGTTTGATGACAGGGGAAGTATGATGTTAAATTAAGGGCACCGGCCGGGGcgcccc                                   >  2:143819/1‑148 (MQ=255)
                                                                                                                   ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                               <  1:143798/148‑1 (MQ=255)
                                                                                                                    tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                             <  2:143799/149‑1 (MQ=255)
                                                                                                                     ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                            <  2:143800/149‑1 (MQ=255)
                                                                                                                       gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTT‑AATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                         <  2:143801/149‑1 (MQ=255)
                                                                                                                        aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                         <  1:143802/149‑1 (MQ=255)
                                                                                                                         aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                        <  1:143804/149‑1 (MQ=255)
                                                                                                                         aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                        <  1:143803/149‑1 (MQ=255)
                                                                                                                           gTTATCCGCCCCCCGCCGCCGCCCCCAAATAAAAATCAAAAGATGAGTGATAATGAATTAGGCTATGGCAAGGTGATCAGATTTTCATAAGAAGGGAAATATGATTTTTAATTAATGGCACCTGCCGGTGCCCCCAATTGGTAAAcaaa                      >  2:143839/1‑149 (MQ=255)
                                                                                                                            ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                      <  1:838431/148‑1 (MQ=255)
                                                                                                                               tCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  >  2:143831/1‑149 (MQ=255)
                                                                                                                                ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa                 <  1:143806/149‑1 (MQ=255)
                                                                                                                                 cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag                <  1:143807/149‑1 (MQ=255)
                                                                                                                                   cccccGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATTAATGGCACCAGCCGGTGCCCCCATTTTTTAAAcaaagcaa                  >  1:1196326/1‑145 (MQ=255)
                                                                                                                                    ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  >  2:143853/1‑144 (MQ=255)
                                                                                                                                     cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAGGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa            <  1:143808/149‑1 (MQ=255)
                                                                                                                                       cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa           >  1:143846/1‑148 (MQ=255)
                                                                                                                                        gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt         >  2:143835/1‑149 (MQ=255)
                                                                                                                                         tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg        <  2:143809/149‑1 (MQ=255)
                                                                                                                                          cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc       >  2:143842/1‑149 (MQ=255)
                                                                                                                                           ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt      >  1:143852/1‑149 (MQ=255)
                                                                                                                                            tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc     <  2:143810/149‑1 (MQ=255)
                                                                                                                                             cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa    <  1:143811/149‑1 (MQ=255)
                                                                                                                                             cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa    <  2:143812/149‑1 (MQ=255)
                                                                                                                                               tccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCGCCCATTTGTTAAACAAAGCAAAGATCAACTGCGCAtt  >  2:143851/1‑149 (MQ=255)
                                                                                                                                               tccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAtt  <  2:143813/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  U00096/422292‑422583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑