Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,537,9490GA22.8% 170.5 / 30.7 79P302L (CCA→CTA) narYnitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base G (26/35);  new base A (18/0);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 4.48e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGT  >  U00096/1537805‑1538092
                                                                                                                                                |                                                                                                                                               
ggCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGCCTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGAt                                                                                                                                              >  1:521246/1‑148 (MQ=255)
ggCAGGGTGCGGTATTCAGGGTGCAACGGTAGGGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATc                                                                                                                                             <  2:521222/149‑1 (MQ=255)
  cAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGAGCACTGAGGGATCGTGTGGATCGa                                                                                                                                           <  2:521224/149‑1 (MQ=255)
  cAGGGTGCGGTATTCAGGGTGCAACGGGAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCGTCCTCGATCACTGAGGGATCGTGTGGATCGa                                                                                                                                           <  1:521223/149‑1 (MQ=255)
     ggTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGa                                                                                                                                        >  1:521286/1‑149 (MQ=255)
         cGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAacac                                                                                                                                    <  2:521225/149‑1 (MQ=255)
         cGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAaca                                                                                                                                     <  1:521226/148‑1 (MQ=255)
            tATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTc                                                                                                                                 <  1:521227/149‑1 (MQ=255)
              ttCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGTAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGc                                                                                                                               <  2:521228/149‑1 (MQ=255)
              ttCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGTAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGc                                                                                                                               <  2:521229/149‑1 (MQ=255)
               tCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCa                                                                                                                              >  2:521257/1‑149 (MQ=255)
                cAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCAc                                                                                                                             >  2:521248/1‑149 (MQ=255)
                 aGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACt                                                                                                                            <  1:521231/149‑1 (MQ=255)
                  gggTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTTCGCAcac                                                                                                                           >  2:521263/1‑147 (MQ=255)
                   ggTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTTGAATAAATTTTTTGTGGGCTTCCTAGATCACTTAGTGATCGTGTGGATCGAGGAACACTTCGCACTgg                                                                                                                          <  2:521232/149‑1 (MQ=255)
                        aaCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTTAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTc                                                                                                                     <  2:521233/149‑1 (MQ=255)
                         aCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATAAATTGTTTCAGGGCTTCCTAGATCTATGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCa                                                                                                                    <  2:521234/149‑1 (MQ=255)
                            gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTTGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTTTTTCAGGGCTTCCTAGATGACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATag                                                                                                                 <  2:521235/149‑1 (MQ=255)
                            gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCAAAGTCGTTCATat                                                                                                                 >  2:521261/1‑148 (MQ=255)
                                    aGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTTGGCAGCGGCAATCACATTTTGTGGAATACCTTGTTGCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAAc                                                                                                         <  1:521236/149‑1 (MQ=255)
                                       ttCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                      >  1:521284/1‑149 (MQ=255)
                                       ttCCAGGCCCTCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGGCAATCACATTTTTTGTATTAACTGGTTTGAGGTCTTCCTAGATCTCTGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                      <  2:521237/149‑1 (MQ=255)
                                         ccAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTcgc                                                                                                    <  1:521238/149‑1 (MQ=255)
                                              ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                               <  2:521239/149‑1 (MQ=255)
                                              ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                               <  2:521240/149‑1 (MQ=255)
                                                     cATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTcg                                                                                        >  1:521264/1‑149 (MQ=255)
                                                       ttttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAATTAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTcgcc                                                                                      >  2:521290/1‑149 (MQ=255)
                                                        tttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAACATAGAGGTCAACTTCGCGCTCGGTGCTcgccg                                                                                     >  2:521242/1‑149 (MQ=255)
                                                        tttGTAGACTGGCGAACGCTGCGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGAAAAGGAGATAATAGAAGTCAACTTCGCGCTCGGTGCTcgccg                                                                                     >  2:521309/1‑149 (MQ=255)
                                                         ttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAATAATAAAGGTCAACTTCGCGCTCGGTGCTcgccgc                                                                                    >  2:521298/1‑149 (MQ=255)
                                                              gACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCt                                                                               >  1:521287/1‑149 (MQ=255)
                                                                cTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCg                                                                             >  1:521277/1‑149 (MQ=255)
                                                                 tGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAATAAAGGTACACTTCGCGCTCGGTGCTCGCCGCTTACTCGa                                                                            >  2:521299/1‑149 (MQ=255)
                                                                   gCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATg                                                                          >  2:521255/1‑149 (MQ=255)
                                                                    cGAACGCTGGGCAGCGTCAATCACATTTCGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATATTAAAAAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGAAGc                                                                         >  2:735117/1‑149 (MQ=255)
                                                                       aCGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAAAACTTCGCACTGGCGTTCATAGCGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGt                                                                      >  1:521302/1‑149 (MQ=255)
                                                                         gCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCg                                                                    >  2:521274/1‑149 (MQ=255)
                                                                               cAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCCCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcg                                                              <  1:521243/149‑1 (MQ=255)
                                                                                aGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                             <  2:521244/149‑1 (MQ=255)
                                                                                  cGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaa                                                           <  2:521246/149‑1 (MQ=255)
                                                                                   gTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                          >  2:521245/1‑149 (MQ=255)
                                                                                    tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                             <  1:521247/145‑1 (MQ=255)
                                                                                    tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                             >  2:521247/1‑145 (MQ=255)
                                                                                    tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                          >  1:521267/1‑148 (MQ=255)
                                                                                     cAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGCACAAGAATAAATAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagc                                                        >  2:521291/1‑149 (MQ=255)
                                                                                      aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                       >  1:521280/1‑149 (MQ=255)
                                                                                      aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAATAAAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                       >  2:521282/1‑149 (MQ=255)
                                                                                       aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                      <  2:521249/149‑1 (MQ=255)
                                                                                       aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                      <  1:521248/149‑1 (MQ=255)
                                                                                       aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAATAAAAAAATTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                      >  2:521273/1‑149 (MQ=255)
                                                                                         cacaTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                    >  1:521271/1‑149 (MQ=255)
                                                                                           caTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTTCGAACAATAGTTCATAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACg                                                  >  2:521270/1‑149 (MQ=255)
                                                                                            aTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGc                                                 >  2:521288/1‑149 (MQ=255)
                                                                                             ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCTGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                <  1:521250/149‑1 (MQ=255)
                                                                                             ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                >  2:521289/1‑149 (MQ=255)
                                                                                                 gtgGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGa                                            <  2:521251/149‑1 (MQ=255)
                                                                                                   ggAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGAGGTCGGCGTCGTAAAGCAGCACGCCCAGATa                                          >  2:521294/1‑149 (MQ=255)
                                                                                                      aTACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCg                                       <  1:521252/149‑1 (MQ=255)
                                                                                                        aCCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTGCATAGAGGGCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGa                                     <  1:521253/149‑1 (MQ=255)
                                                                                                         ccTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGAt                                    <  2:521254/149‑1 (MQ=255)
                                                                                                          cTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGGTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATg                                   >  1:521292/1‑149 (MQ=255)
                                                                                                           ttGGTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGCACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGc                                  >  1:521276/1‑149 (MQ=255)
                                                                                                              tttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTACAGCCGCACGCCCAGATCCCGGAGGCGAc                               >  1:1202797/1‑149 (MQ=255)
                                                                                                               ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGa                                <  1:521255/147‑1 (MQ=255)
                                                                                                               ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTAGCCGCTGACTCTCTGCGGTCGGCGTCGTAAATCGGCTCGCCCAGATACCGGATGAGAca                              >  2:521304/1‑148 (MQ=255)
                                                                                                                   gggCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACg                          >  1:521285/1‑149 (MQ=255)
                                                                                                                    ggCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGc                         >  2:521311/1‑149 (MQ=255)
                                                                                                                      cTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAg                       <  1:521256/149‑1 (MQ=255)
                                                                                                                       ttCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                      >  1:521281/1‑149 (MQ=255)
                                                                                                                       ttCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                      >  2:521301/1‑149 (MQ=255)
                                                                                                                         ccTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGtt                    <  1:521257/149‑1 (MQ=255)
                                                                                                                          cTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGttt                   >  1:521283/1‑149 (MQ=255)
                                                                                                                           tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                    <  2:521258/115‑1 (MQ=255)
                                                                                                                           tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                    >  1:521258/1‑115 (MQ=255)
                                                                                                                                cACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGc             <  1:521259/149‑1 (MQ=255)
                                                                                                                                 aCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGca            >  1:521275/1‑149 (MQ=255)
                                                                                                                                        gATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtc     >  1:521300/1‑149 (MQ=255)
                                                                                                                                           cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATATAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt  <  1:521261/149‑1 (MQ=255)
                                                                                                                                           cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt  >  1:521303/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GGCAGGGTGCGGTATTCAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGT  >  U00096/1537805‑1538092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑