Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,537,9690CA21.3% 181.1 / 18.0 75Q295H (CAG→CAT) ‡narYnitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base A (16/0);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 3.39e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAAC  >  U00096/1537821‑1538117
                                                                                                                                                    |                                                                                                                                                    
cAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCAc                                                                                                                                                      >  2:521248/1‑149 (MQ=255)
 aGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACt                                                                                                                                                     <  1:521231/149‑1 (MQ=255)
  gggTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTTCGCAcac                                                                                                                                                    >  2:521263/1‑147 (MQ=255)
   ggTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTTGAATAAATTTTTTGTGGGCTTCCTAGATCACTTAGTGATCGTGTGGATCGAGGAACACTTCGCACTgg                                                                                                                                                   <  2:521232/149‑1 (MQ=255)
        aaCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTTAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTc                                                                                                                                              <  2:521233/149‑1 (MQ=255)
         aCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATAAATTGTTTCAGGGCTTCCTAGATCTATGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCa                                                                                                                                             <  2:521234/149‑1 (MQ=255)
            gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTTGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTTTTTCAGGGCTTCCTAGATGACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATag                                                                                                                                          <  2:521235/149‑1 (MQ=255)
            gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCAAAGTCGTTCATat                                                                                                                                          >  2:521261/1‑148 (MQ=255)
                    aGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTTGGCAGCGGCAATCACATTTTGTGGAATACCTTGTTGCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAAc                                                                                                                                  <  1:521236/149‑1 (MQ=255)
                       ttCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                               >  1:521284/1‑149 (MQ=255)
                       ttCCAGGCCCTCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGGCAATCACATTTTTTGTATTAACTGGTTTGAGGTCTTCCTAGATCTCTGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                               <  2:521237/149‑1 (MQ=255)
                         ccAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTcgc                                                                                                                             <  1:521238/149‑1 (MQ=255)
                              ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                        <  2:521240/149‑1 (MQ=255)
                              ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                        <  2:521239/149‑1 (MQ=255)
                                     cATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTcg                                                                                                                 >  1:521264/1‑149 (MQ=255)
                                       ttttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAATTAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTcgcc                                                                                                               >  2:521290/1‑149 (MQ=255)
                                        tttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAACATAGAGGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                              >  2:521242/1‑149 (MQ=255)
                                        tttGTAGACTGGCGAACGCTGCGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGAAAAGGAGATAATAGAAGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                              >  2:521309/1‑149 (MQ=255)
                                         ttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAATAATAAAGGTCAACTTCGCGCTCGGTGCTcgccgc                                                                                                             >  2:521298/1‑149 (MQ=255)
                                              gACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCt                                                                                                        >  1:521287/1‑149 (MQ=255)
                                                cTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCg                                                                                                      >  1:521277/1‑149 (MQ=255)
                                                 tGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAATAAAGGTACACTTCGCGCTCGGTGCTCGCCGCTTACTCGa                                                                                                     >  2:521299/1‑149 (MQ=255)
                                                   gCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATg                                                                                                   >  2:521255/1‑149 (MQ=255)
                                                    cGAACGCTGGGCAGCGTCAATCACATTTCGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATATTAAAAAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGAAGc                                                                                                  >  2:735117/1‑149 (MQ=255)
                                                       aCGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAAAACTTCGCACTGGCGTTCATAGCGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGt                                                                                               >  1:521302/1‑149 (MQ=255)
                                                         gCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCg                                                                                             >  2:521274/1‑149 (MQ=255)
                                                               cAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCCCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcg                                                                                       <  1:521243/149‑1 (MQ=255)
                                                                aGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                      <  2:521244/149‑1 (MQ=255)
                                                                  cGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaa                                                                                    <  2:521246/149‑1 (MQ=255)
                                                                   gTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                   >  2:521245/1‑149 (MQ=255)
                                                                    tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                      >  2:521247/1‑145 (MQ=255)
                                                                    tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                      <  1:521247/145‑1 (MQ=255)
                                                                    tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                   >  1:521267/1‑148 (MQ=255)
                                                                     cAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGCACAAGAATAAATAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagc                                                                                 >  2:521291/1‑149 (MQ=255)
                                                                      aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                >  1:521280/1‑149 (MQ=255)
                                                                      aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAATAAAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                >  2:521282/1‑149 (MQ=255)
                                                                       aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                               <  2:521249/149‑1 (MQ=255)
                                                                       aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                               <  1:521248/149‑1 (MQ=255)
                                                                       aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAATAAAAAAATTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                               >  2:521273/1‑149 (MQ=255)
                                                                         cacaTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                             >  1:521271/1‑149 (MQ=255)
                                                                           caTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTTCGAACAATAGTTCATAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACg                                                                           >  2:521270/1‑149 (MQ=255)
                                                                            aTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGc                                                                          >  2:521288/1‑149 (MQ=255)
                                                                             ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCTGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                         <  1:521250/149‑1 (MQ=255)
                                                                             ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                         >  2:521289/1‑149 (MQ=255)
                                                                                 gtgGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGa                                                                     <  2:521251/149‑1 (MQ=255)
                                                                                   ggAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGAGGTCGGCGTCGTAAAGCAGCACGCCCAGATa                                                                   >  2:521294/1‑149 (MQ=255)
                                                                                      aTACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCg                                                                <  1:521252/149‑1 (MQ=255)
                                                                                        aCCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTGCATAGAGGGCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGa                                                              <  1:521253/149‑1 (MQ=255)
                                                                                         ccTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGAt                                                             <  2:521254/149‑1 (MQ=255)
                                                                                          cTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGGTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATg                                                            >  1:521292/1‑149 (MQ=255)
                                                                                           ttGGTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGCACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGc                                                           >  1:521276/1‑149 (MQ=255)
                                                                                              tttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTACAGCCGCACGCCCAGATCCCGGAGGCGAc                                                        >  1:1202797/1‑149 (MQ=255)
                                                                                               ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGa                                                         <  1:521255/147‑1 (MQ=255)
                                                                                               ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTAGCCGCTGACTCTCTGCGGTCGGCGTCGTAAATCGGCTCGCCCAGATACCGGATGAGAca                                                       >  2:521304/1‑148 (MQ=255)
                                                                                                   gggCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACg                                                   >  1:521285/1‑149 (MQ=255)
                                                                                                    ggCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGc                                                  >  2:521311/1‑149 (MQ=255)
                                                                                                      cTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAg                                                <  1:521256/149‑1 (MQ=255)
                                                                                                       ttCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                               >  1:521281/1‑149 (MQ=255)
                                                                                                       ttCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                               >  2:521301/1‑149 (MQ=255)
                                                                                                         ccTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGtt                                             <  1:521257/149‑1 (MQ=255)
                                                                                                          cTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGttt                                            >  1:521283/1‑149 (MQ=255)
                                                                                                           tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                             <  2:521258/115‑1 (MQ=255)
                                                                                                           tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                             >  1:521258/1‑115 (MQ=255)
                                                                                                                cACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGc                                      <  1:521259/149‑1 (MQ=255)
                                                                                                                 aCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGca                                     >  1:521275/1‑149 (MQ=255)
                                                                                                                        gATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtc                              >  1:521300/1‑149 (MQ=255)
                                                                                                                           cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATATAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                           <  1:521261/149‑1 (MQ=255)
                                                                                                                           cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                           >  1:521303/1‑149 (MQ=255)
                                                                                                                                 gATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCg                     <  1:521262/149‑1 (MQ=255)
                                                                                                                                     gAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGACGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCAAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACAGGACt                 >  2:521318/1‑149 (MQ=255)
                                                                                                                                      aGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggt                               <  1:521263/134‑1 (MQ=255)
                                                                                                                                         aaCACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAt             >  2:521296/1‑149 (MQ=255)
                                                                                                                                          acacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAtt            <  2:521264/149‑1 (MQ=255)
                                                                                                                                           cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGCTACCGGATGCGACCCACGCAg                                                >  1:521295/1‑112 (MQ=255)
                                                                                                                                           cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc           <  1:521265/149‑1 (MQ=255)
                                                                                                                                            acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGCCCGGACTCAATTCg          >  1:521308/1‑149 (MQ=255)
                                                                                                                                            acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc           <  1:521266/148‑1 (MQ=255)
                                                                                                                                                    cTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAAc  >  1:521297/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGGGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAAC  >  U00096/1537821‑1538117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑