Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,537,9720GT21.6% 182.1 / 19.3 74R294R (CGC→CGA) ‡narYnitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base T (16/0);  total (44/30)
Fisher's exact test for biased strand distribution p-value = 8.34e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAG  >  U00096/1537824‑1538119
                                                                                                                                                    |                                                                                                                                                   
ggTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTTGAATAAATTTTTTGTGGGCTTCCTAGATCACTTAGTGATCGTGTGGATCGAGGAACACTTCGCACTgg                                                                                                                                                     <  2:521232/149‑1 (MQ=255)
     aaCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTTAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTc                                                                                                                                                <  2:521233/149‑1 (MQ=255)
      aCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATAAATTGTTTCAGGGCTTCCTAGATCTATGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCa                                                                                                                                               <  2:521234/149‑1 (MQ=255)
         gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTTGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTTTTTCAGGGCTTCCTAGATGACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATag                                                                                                                                            <  2:521235/149‑1 (MQ=255)
         gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCAAAGTCGTTCATat                                                                                                                                            >  2:521261/1‑148 (MQ=255)
                 aGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTTGGCAGCGGCAATCACATTTTGTGGAATACCTTGTTGCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAAc                                                                                                                                    <  1:521236/149‑1 (MQ=255)
                    ttCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                                 >  1:521284/1‑149 (MQ=255)
                    ttCCAGGCCCTCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGGCAATCACATTTTTTGTATTAACTGGTTTGAGGTCTTCCTAGATCTCTGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                                 <  2:521237/149‑1 (MQ=255)
                      ccAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTcgc                                                                                                                               <  1:521238/149‑1 (MQ=255)
                           ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                          <  2:521240/149‑1 (MQ=255)
                           ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                          <  2:521239/149‑1 (MQ=255)
                                  cATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTcg                                                                                                                   >  1:521264/1‑149 (MQ=255)
                                    ttttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAATTAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTcgcc                                                                                                                 >  2:521290/1‑149 (MQ=255)
                                     tttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAACATAGAGGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                                >  2:521242/1‑149 (MQ=255)
                                     tttGTAGACTGGCGAACGCTGCGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGAAAAGGAGATAATAGAAGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                                >  2:521309/1‑149 (MQ=255)
                                      ttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAATAATAAAGGTCAACTTCGCGCTCGGTGCTcgccgc                                                                                                               >  2:521298/1‑149 (MQ=255)
                                           gACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCt                                                                                                          >  1:521287/1‑149 (MQ=255)
                                             cTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCg                                                                                                        >  1:521277/1‑149 (MQ=255)
                                              tGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAATAAAGGTACACTTCGCGCTCGGTGCTCGCCGCTTACTCGa                                                                                                       >  2:521299/1‑149 (MQ=255)
                                                gCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATg                                                                                                     >  2:521255/1‑149 (MQ=255)
                                                 cGAACGCTGGGCAGCGTCAATCACATTTCGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATATTAAAAAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGAAGc                                                                                                    >  2:735117/1‑149 (MQ=255)
                                                    aCGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAAAACTTCGCACTGGCGTTCATAGCGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGt                                                                                                 >  1:521302/1‑149 (MQ=255)
                                                      gCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCg                                                                                               >  2:521274/1‑149 (MQ=255)
                                                            cAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCCCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcg                                                                                         <  1:521243/149‑1 (MQ=255)
                                                             aGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                        <  2:521244/149‑1 (MQ=255)
                                                               cGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaa                                                                                      <  2:521246/149‑1 (MQ=255)
                                                                gTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                     >  2:521245/1‑149 (MQ=255)
                                                                 tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                        <  1:521247/145‑1 (MQ=255)
                                                                 tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                        >  2:521247/1‑145 (MQ=255)
                                                                 tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                     >  1:521267/1‑148 (MQ=255)
                                                                  cAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGCACAAGAATAAATAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagc                                                                                   >  2:521291/1‑149 (MQ=255)
                                                                   aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                  >  1:521280/1‑149 (MQ=255)
                                                                   aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAATAAAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                  >  2:521282/1‑149 (MQ=255)
                                                                    aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                 <  1:521248/149‑1 (MQ=255)
                                                                    aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                 <  2:521249/149‑1 (MQ=255)
                                                                    aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAATAAAAAAATTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                 >  2:521273/1‑149 (MQ=255)
                                                                      cacaTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                               >  1:521271/1‑149 (MQ=255)
                                                                        caTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTTCGAACAATAGTTCATAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACg                                                                             >  2:521270/1‑149 (MQ=255)
                                                                         aTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGc                                                                            >  2:521288/1‑149 (MQ=255)
                                                                          ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCTGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                           <  1:521250/149‑1 (MQ=255)
                                                                          ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                           >  2:521289/1‑149 (MQ=255)
                                                                              gtgGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGa                                                                       <  2:521251/149‑1 (MQ=255)
                                                                                ggAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGAGGTCGGCGTCGTAAAGCAGCACGCCCAGATa                                                                     >  2:521294/1‑149 (MQ=255)
                                                                                   aTACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCg                                                                  <  1:521252/149‑1 (MQ=255)
                                                                                     aCCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTGCATAGAGGGCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGa                                                                <  1:521253/149‑1 (MQ=255)
                                                                                      ccTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGAt                                                               <  2:521254/149‑1 (MQ=255)
                                                                                       cTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGGTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATg                                                              >  1:521292/1‑149 (MQ=255)
                                                                                        ttGGTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGCACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGc                                                             >  1:521276/1‑149 (MQ=255)
                                                                                           tttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTACAGCCGCACGCCCAGATCCCGGAGGCGAc                                                          >  1:1202797/1‑149 (MQ=255)
                                                                                            ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGa                                                           <  1:521255/147‑1 (MQ=255)
                                                                                            ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTAGCCGCTGACTCTCTGCGGTCGGCGTCGTAAATCGGCTCGCCCAGATACCGGATGAGAca                                                         >  2:521304/1‑148 (MQ=255)
                                                                                                gggCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACg                                                     >  1:521285/1‑149 (MQ=255)
                                                                                                 ggCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGc                                                    >  2:521311/1‑149 (MQ=255)
                                                                                                   cTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAg                                                  <  1:521256/149‑1 (MQ=255)
                                                                                                    ttCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                                 >  1:521281/1‑149 (MQ=255)
                                                                                                    ttCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                                 >  2:521301/1‑149 (MQ=255)
                                                                                                      ccTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGtt                                               <  1:521257/149‑1 (MQ=255)
                                                                                                       cTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGttt                                              >  1:521283/1‑149 (MQ=255)
                                                                                                        tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                               <  2:521258/115‑1 (MQ=255)
                                                                                                        tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                               >  1:521258/1‑115 (MQ=255)
                                                                                                             cACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGc                                        <  1:521259/149‑1 (MQ=255)
                                                                                                              aCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGca                                       >  1:521275/1‑149 (MQ=255)
                                                                                                                     gATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtc                                >  1:521300/1‑149 (MQ=255)
                                                                                                                        cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATATAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                             <  1:521261/149‑1 (MQ=255)
                                                                                                                        cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                             >  1:521303/1‑149 (MQ=255)
                                                                                                                              gATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCg                       <  1:521262/149‑1 (MQ=255)
                                                                                                                                  gAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGACGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCAAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACAGGACt                   >  2:521318/1‑149 (MQ=255)
                                                                                                                                   aGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggt                                 <  1:521263/134‑1 (MQ=255)
                                                                                                                                      aaCACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAt               >  2:521296/1‑149 (MQ=255)
                                                                                                                                       acacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAtt              <  2:521264/149‑1 (MQ=255)
                                                                                                                                        cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGCTACCGGATGCGACCCACGCAg                                                  >  1:521295/1‑112 (MQ=255)
                                                                                                                                        cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc             <  1:521265/149‑1 (MQ=255)
                                                                                                                                         acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGCCCGGACTCAATTCg            >  1:521308/1‑149 (MQ=255)
                                                                                                                                         acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc             <  1:521266/148‑1 (MQ=255)
                                                                                                                                                 cTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAAc    >  1:521297/1‑149 (MQ=255)
                                                                                                                                                   ggCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACag  <  2:521267/149‑1 (MQ=255)
                                                                                                                                                   ggCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACag  >  2:521328/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTGCAACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAG  >  U00096/1537824‑1538119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑