Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,537,9730CA22.4% 185.0 / 31.7 76R294L (CGC→CTC) ‡narYnitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base A (17/0);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 3.70e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAA  >  U00096/1537829‑1538121
                                                                                                                                                |                                                                                                                                                    
aaCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTTAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTc                                                                                                                                                  <  2:521233/149‑1 (MQ=255)
 aCGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATAAATTGTTTCAGGGCTTCCTAGATCTATGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCa                                                                                                                                                 <  2:521234/149‑1 (MQ=255)
    gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTTGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTTTTTCAGGGCTTCCTAGATGACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATag                                                                                                                                              <  2:521235/149‑1 (MQ=255)
    gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCAAAGTCGTTCATat                                                                                                                                              >  2:521261/1‑148 (MQ=255)
            aGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTTGGCAGCGGCAATCACATTTTGTGGAATACCTTGTTGCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAAc                                                                                                                                      <  1:521236/149‑1 (MQ=255)
               ttCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                                   >  1:521284/1‑149 (MQ=255)
               ttCCAGGCCCTCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGGCAATCACATTTTTTGTATTAACTGGTTTGAGGTCTTCCTAGATCTCTGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                                   <  2:521237/149‑1 (MQ=255)
                 ccAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTcgc                                                                                                                                 <  1:521238/149‑1 (MQ=255)
                      ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                            <  2:521240/149‑1 (MQ=255)
                      ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                            <  2:521239/149‑1 (MQ=255)
                             cATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTcg                                                                                                                     >  1:521264/1‑149 (MQ=255)
                               ttttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAATTAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTcgcc                                                                                                                   >  2:521290/1‑149 (MQ=255)
                                tttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAACATAGAGGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                                  >  2:521242/1‑149 (MQ=255)
                                tttGTAGACTGGCGAACGCTGCGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGAAAAGGAGATAATAGAAGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                                  >  2:521309/1‑149 (MQ=255)
                                 ttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAATAATAAAGGTCAACTTCGCGCTCGGTGCTcgccgc                                                                                                                 >  2:521298/1‑149 (MQ=255)
                                      gACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCt                                                                                                            >  1:521287/1‑149 (MQ=255)
                                        cTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCg                                                                                                          >  1:521277/1‑149 (MQ=255)
                                         tGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAATAAAGGTACACTTCGCGCTCGGTGCTCGCCGCTTACTCGa                                                                                                         >  2:521299/1‑149 (MQ=255)
                                           gCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATg                                                                                                       >  2:521255/1‑149 (MQ=255)
                                            cGAACGCTGGGCAGCGTCAATCACATTTCGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATATTAAAAAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGAAGc                                                                                                      >  2:735117/1‑149 (MQ=255)
                                               aCGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAAAACTTCGCACTGGCGTTCATAGCGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGt                                                                                                   >  1:521302/1‑149 (MQ=255)
                                                 gCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCg                                                                                                 >  2:521274/1‑149 (MQ=255)
                                                       cAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCCCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcg                                                                                           <  1:521243/149‑1 (MQ=255)
                                                        aGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                          <  2:521244/149‑1 (MQ=255)
                                                          cGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaa                                                                                        <  2:521246/149‑1 (MQ=255)
                                                           gTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                       >  2:521245/1‑149 (MQ=255)
                                                            tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                          >  2:521247/1‑145 (MQ=255)
                                                            tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                          <  1:521247/145‑1 (MQ=255)
                                                            tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                       >  1:521267/1‑148 (MQ=255)
                                                             cAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGCACAAGAATAAATAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagc                                                                                     >  2:521291/1‑149 (MQ=255)
                                                              aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                    >  1:521280/1‑149 (MQ=255)
                                                              aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAATAAAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                    >  2:521282/1‑149 (MQ=255)
                                                               aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                   <  1:521248/149‑1 (MQ=255)
                                                               aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                   <  2:521249/149‑1 (MQ=255)
                                                               aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAATAAAAAAATTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                   >  2:521273/1‑149 (MQ=255)
                                                                 cacaTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                                 >  1:521271/1‑149 (MQ=255)
                                                                   caTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTTCGAACAATAGTTCATAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACg                                                                               >  2:521270/1‑149 (MQ=255)
                                                                    aTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGc                                                                              >  2:521288/1‑149 (MQ=255)
                                                                     ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCTGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                             <  1:521250/149‑1 (MQ=255)
                                                                     ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                             >  2:521289/1‑149 (MQ=255)
                                                                         gtgGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGa                                                                         <  2:521251/149‑1 (MQ=255)
                                                                           ggAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGAGGTCGGCGTCGTAAAGCAGCACGCCCAGATa                                                                       >  2:521294/1‑149 (MQ=255)
                                                                              aTACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCg                                                                    <  1:521252/149‑1 (MQ=255)
                                                                                aCCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTGCATAGAGGGCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGa                                                                  <  1:521253/149‑1 (MQ=255)
                                                                                 ccTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGAt                                                                 <  2:521254/149‑1 (MQ=255)
                                                                                  cTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGGTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATg                                                                >  1:521292/1‑149 (MQ=255)
                                                                                   ttGGTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGCACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGc                                                               >  1:521276/1‑149 (MQ=255)
                                                                                      tttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTACAGCCGCACGCCCAGATCCCGGAGGCGAc                                                            >  1:1202797/1‑149 (MQ=255)
                                                                                       ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGa                                                             <  1:521255/147‑1 (MQ=255)
                                                                                       ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTAGCCGCTGACTCTCTGCGGTCGGCGTCGTAAATCGGCTCGCCCAGATACCGGATGAGAca                                                           >  2:521304/1‑148 (MQ=255)
                                                                                           gggCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACg                                                       >  1:521285/1‑149 (MQ=255)
                                                                                            ggCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGc                                                      >  2:521311/1‑149 (MQ=255)
                                                                                              cTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAg                                                    <  1:521256/149‑1 (MQ=255)
                                                                                               ttCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                                   >  1:521281/1‑149 (MQ=255)
                                                                                               ttCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                                   >  2:521301/1‑149 (MQ=255)
                                                                                                 ccTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGtt                                                 <  1:521257/149‑1 (MQ=255)
                                                                                                  cTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGttt                                                >  1:521283/1‑149 (MQ=255)
                                                                                                   tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                                 >  1:521258/1‑115 (MQ=255)
                                                                                                   tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                                 <  2:521258/115‑1 (MQ=255)
                                                                                                        cACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGc                                          <  1:521259/149‑1 (MQ=255)
                                                                                                         aCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGca                                         >  1:521275/1‑149 (MQ=255)
                                                                                                                gATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtc                                  >  1:521300/1‑149 (MQ=255)
                                                                                                                   cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATATAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                               <  1:521261/149‑1 (MQ=255)
                                                                                                                   cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                               >  1:521303/1‑149 (MQ=255)
                                                                                                                         gATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCg                         <  1:521262/149‑1 (MQ=255)
                                                                                                                             gAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGACGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCAAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACAGGACt                     >  2:521318/1‑149 (MQ=255)
                                                                                                                              aGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggt                                   <  1:521263/134‑1 (MQ=255)
                                                                                                                                 aaCACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAt                 >  2:521296/1‑149 (MQ=255)
                                                                                                                                  acacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAtt                <  2:521264/149‑1 (MQ=255)
                                                                                                                                   cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGCTACCGGATGCGACCCACGCAg                                                    >  1:521295/1‑112 (MQ=255)
                                                                                                                                   cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc               <  1:521265/149‑1 (MQ=255)
                                                                                                                                    acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGCCCGGACTCAATTCg              >  1:521308/1‑149 (MQ=255)
                                                                                                                                    acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc               <  1:521266/148‑1 (MQ=255)
                                                                                                                                            cTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAAc      >  1:521297/1‑149 (MQ=255)
                                                                                                                                              ggCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACag    <  2:521267/149‑1 (MQ=255)
                                                                                                                                              ggCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACag    >  2:521328/1‑149 (MQ=255)
                                                                                                                                                cGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACagaa  <  2:521268/149‑1 (MQ=255)
                                                                                                                                                cGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACagaa  <  1:521269/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AACGGTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAA  >  U00096/1537829‑1538121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑