Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,537,9810GA20.0% 183.4 / 21.1 76L291L (CTC→CTT) narYnitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base G (29/31);  new base A (15/0);  total (44/32)
Fisher's exact test for biased strand distribution p-value = 1.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAAGATGCATT  >  U00096/1537833‑1538129
                                                                                                                                                    |                                                                                                                                                    
gTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTTGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTTTTTCAGGGCTTCCTAGATGACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATag                                                                                                                                                      <  2:521235/149‑1 (MQ=255)
        aGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTTGGCAGCGGCAATCACATTTTGTGGAATACCTTGTTGCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAAc                                                                                                                                              <  1:521236/149‑1 (MQ=255)
           ttCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                                           >  1:521284/1‑149 (MQ=255)
           ttCCAGGCCCTCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGGCAATCACATTTTTTGTATTAACTGGTTTGAGGTCTTCCTAGATCTCTGAGTGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTc                                                                                                                                           <  2:521237/149‑1 (MQ=255)
             ccAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTcgc                                                                                                                                         <  1:521238/149‑1 (MQ=255)
                  ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                                    <  2:521239/149‑1 (MQ=255)
                  ccATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCg                                                                                                                                    <  2:521240/149‑1 (MQ=255)
                         cATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTcg                                                                                                                             >  1:521264/1‑149 (MQ=255)
                           ttttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAATTAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTcgcc                                                                                                                           >  2:521290/1‑149 (MQ=255)
                            tttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAACATAGAGGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                                          >  2:521242/1‑149 (MQ=255)
                            tttGTAGACTGGCGAACGCTGCGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGAAAAGGAGATAATAGAAGTCAACTTCGCGCTCGGTGCTcgccg                                                                                                                          >  2:521309/1‑149 (MQ=255)
                             ttGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAATAATAAAGGTCAACTTCGCGCTCGGTGCTcgccgc                                                                                                                         >  2:521298/1‑149 (MQ=255)
                                  gACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCt                                                                                                                    >  1:521287/1‑149 (MQ=255)
                                    cTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCg                                                                                                                  >  1:521277/1‑149 (MQ=255)
                                     tGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAATAAAGGTACACTTCGCGCTCGGTGCTCGCCGCTTACTCGa                                                                                                                 >  2:521299/1‑149 (MQ=255)
                                       gCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATg                                                                                                               >  2:521255/1‑149 (MQ=255)
                                        cGAACGCTGGGCAGCGTCAATCACATTTCGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATATTAAAAAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGAAGc                                                                                                              >  2:735117/1‑149 (MQ=255)
                                           aCGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAAAACTTCGCACTGGCGTTCATAGCGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGt                                                                                                           >  1:521302/1‑149 (MQ=255)
                                             gCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCg                                                                                                         >  2:521274/1‑149 (MQ=255)
                                                   cAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCCCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcg                                                                                                   <  1:521243/149‑1 (MQ=255)
                                                    aGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                                  <  2:521244/149‑1 (MQ=255)
                                                      cGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaa                                                                                                <  2:521246/149‑1 (MQ=255)
                                                       gTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                               >  2:521245/1‑149 (MQ=255)
                                                        tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                                  >  2:521247/1‑145 (MQ=255)
                                                        tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGcgtcgt                                                                                                  <  1:521247/145‑1 (MQ=255)
                                                        tCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTaaa                                                                                               >  1:521267/1‑148 (MQ=255)
                                                         cAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCGAGGAACACTACGCACAAGAATAAATAAACGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagc                                                                                             >  2:521291/1‑149 (MQ=255)
                                                          aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                            >  1:521280/1‑149 (MQ=255)
                                                          aaTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAATAAAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagca                                                                                            >  2:521282/1‑149 (MQ=255)
                                                           aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                           <  1:521248/149‑1 (MQ=255)
                                                           aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                           <  2:521249/149‑1 (MQ=255)
                                                           aTCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAATAAAAAAATTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAagcag                                                                                           >  2:521273/1‑149 (MQ=255)
                                                             cacaTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                                         >  1:521271/1‑149 (MQ=255)
                                                               caTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTTCGAACAATAGTTCATAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACg                                                                                       >  2:521270/1‑149 (MQ=255)
                                                                aTTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGc                                                                                      >  2:521288/1‑149 (MQ=255)
                                                                 ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCTGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                                     <  1:521250/149‑1 (MQ=255)
                                                                 ttttGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAATTAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGcc                                                                                     >  2:521289/1‑149 (MQ=255)
                                                                     gtgGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGa                                                                                 <  2:521251/149‑1 (MQ=255)
                                                                       ggAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGAGGTCGGCGTCGTAAAGCAGCACGCCCAGATa                                                                               >  2:521294/1‑149 (MQ=255)
                                                                          aTACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCg                                                                            <  1:521252/149‑1 (MQ=255)
                                                                            aCCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTGCATAGAGGGCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGa                                                                          <  1:521253/149‑1 (MQ=255)
                                                                             ccTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGAt                                                                         <  2:521254/149‑1 (MQ=255)
                                                                              cTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGGTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATg                                                                        >  1:521292/1‑149 (MQ=255)
                                                                               ttGGTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGCACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGc                                                                       >  1:521276/1‑149 (MQ=255)
                                                                                  tttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTACAGCCGCACGCCCAGATCCCGGAGGCGAc                                                                    >  1:1202797/1‑149 (MQ=255)
                                                                                   ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGa                                                                     <  1:521255/147‑1 (MQ=255)
                                                                                   ttCAGGGCTTCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGCAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTAGCCGCTGACTCTCTGCGGTCGGCGTCGTAAATCGGCTCGCCCAGATACCGGATGAGAca                                                                   >  2:521304/1‑148 (MQ=255)
                                                                                       gggCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACg                                                               >  1:521285/1‑149 (MQ=255)
                                                                                        ggCTTCCTCGATCACTGAGGGATCGTGTAGATCTCGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGc                                                              >  2:521311/1‑149 (MQ=255)
                                                                                          cTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAg                                                            <  1:521256/149‑1 (MQ=255)
                                                                                           ttCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                                           >  1:521281/1‑149 (MQ=255)
                                                                                           ttCCTCGATCACTGAGGGATCGTGTAGATCTAGGAACACTACGAAAAATAAAAAAAAAAAGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAgg                                                           >  2:521301/1‑149 (MQ=255)
                                                                                             ccTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGtt                                                         <  1:521257/149‑1 (MQ=255)
                                                                                              cTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGttt                                                        >  1:521283/1‑149 (MQ=255)
                                                                                               tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                                         >  1:521258/1‑115 (MQ=255)
                                                                                               tCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAgcagca                                                                                         <  2:521258/115‑1 (MQ=255)
                                                                                                    cACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGc                                                  <  1:521259/149‑1 (MQ=255)
                                                                                                     aCTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGca                                                 >  1:521275/1‑149 (MQ=255)
                                                                                                            gATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtc                                          >  1:521300/1‑149 (MQ=255)
                                                                                                               cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATATAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                                       <  1:521261/149‑1 (MQ=255)
                                                                                                               cGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggtcggt                                       >  1:521303/1‑149 (MQ=255)
                                                                                                                     gATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCg                                 <  1:521262/149‑1 (MQ=255)
                                                                                                                         gAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGACGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCAAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACAGGACt                             >  2:521318/1‑149 (MQ=255)
                                                                                                                          aGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACAcggt                                           <  1:521263/134‑1 (MQ=255)
                                                                                                                             aaCACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAt                         >  2:521296/1‑149 (MQ=255)
                                                                                                                              acacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAAtt                        <  2:521264/149‑1 (MQ=255)
                                                                                                                               cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGCTACCGGATGCGACCCACGCAg                                                            >  1:521295/1‑112 (MQ=255)
                                                                                                                               cacTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc                       <  1:521265/149‑1 (MQ=255)
                                                                                                                                acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGCCCGGACTCAATTCg                      >  1:521308/1‑149 (MQ=255)
                                                                                                                                acTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTc                       <  1:521266/148‑1 (MQ=255)
                                                                                                                                        cTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAAc              >  1:521297/1‑149 (MQ=255)
                                                                                                                                          ggCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACag            >  2:521328/1‑149 (MQ=255)
                                                                                                                                          ggCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACag            <  2:521267/149‑1 (MQ=255)
                                                                                                                                            cGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACagaa          <  1:521269/149‑1 (MQ=255)
                                                                                                                                            cGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACagaa          <  2:521268/149‑1 (MQ=255)
                                                                                                                                              ttCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACagaaga        <  1:521270/149‑1 (MQ=255)
                                                                                                                                                cATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAAGATg      <  2:521271/149‑1 (MQ=255)
                                                                                                                                                   tgagGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAATGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAAGATGCAt   <  2:521272/148‑1 (MQ=255)
                                                                                                                                                    gagGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAAGATGCAtt  >  2:521334/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTAGTGCCAGTTTCCAGTCCATCGCCATTTTGTAGACTGGCGAACGCTGGGCAGCGTCAATCACATTTTGTGGAATACCTTGTTTCAGGGCTTCCTCGATCACTGAGGGATCGTGTGGATCGAGGAACACTTCGCACTGGCGTTCATAGAGGTCAACTTCGCGCTCGGTGCTCGCCGCTTCCTCGATGCGGTCGGCGTCGTAAAGCAGCACGCCCAGATACCGGATGCGACCCACGCAGGTTTCTGAGCACACGGTCGGTTGACCGGACTCAATTCGTGGGTAACAGAAGATGCATT  >  U00096/1537833‑1538129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑