Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 2,713,364 G→A D157N (GAC→AAC)  srmB → ATP‑dependent RNA helicase SrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000962,713,3640GA99.6% 902.3 / NA 234D157N (GAC→AAC) srmBATP‑dependent RNA helicase SrmB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (114/119);  minor base C (0/1);  total (114/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

CCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGA  >  U00096/2713218‑2713509
                                                                                                                                                  |                                                                                                                                                 
ccaccaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTcaac                                                                                                                                                 >  2:2396155/1‑149 (MQ=255)
  accaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCCGCGAAATTCAGGACATCGTGGTCGCCCCGCCCGGCCGTCTGCTGCAATACATAAAAGAAGAGCCCTTCGATTGCCGCGCGGTTGAAACGCTGATCCTcaacga                                                                                                                                               >  1:2396293/1‑149 (MQ=255)
  accaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTcaacga                                                                                                                                               <  2:2396097/149‑1 (MQ=255)
  accaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTCAAACGCTGATCCTcaacga                                                                                                                                               <  1:2396098/149‑1 (MQ=255)
  accaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGGCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTcaacga                                                                                                                                               <  1:2396095/149‑1 (MQ=255)
   ccaTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGaa                                                                                                                                              >  2:2396258/1‑149 (MQ=255)
    caTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAg                                                                                                                                             >  2:2396176/1‑149 (MQ=255)
     aTCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGc                                                                                                                                            <  2:2396100/149‑1 (MQ=255)
         ccGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGAc                                                                                                                                        <  1:2396103/149‑1 (MQ=255)
            gcggcgTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAACCGCTGATCCTCAACGAAGCAGACCGt                                                                                                                                     >  2:2396287/1‑149 (MQ=255)
            gcggcgTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGt                                                                                                                                     >  2:2396275/1‑149 (MQ=255)
             cggcgTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTa                                                                                                                                    <  2:2396106/149‑1 (MQ=255)
                  tAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTg                                                                                                                               <  1:2396110/149‑1 (MQ=255)
                  tAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTg                                                                                                                               <  2:2396109/149‑1 (MQ=255)
                    gCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGa                                                                                                                             >  1:2396239/1‑149 (MQ=255)
                    gCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGa                                                                                                                             <  2:2396114/149‑1 (MQ=255)
                    gCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGa                                                                                                                             >  1:2396125/1‑149 (MQ=255)
                     ccTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGa                                                                                                                             <  1:2396115/148‑1 (MQ=255)
                      cTATATGAACCACGCGGACGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGata                                                                                                                           <  2:2396119/149‑1 (MQ=255)
                        atatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATg                                                                                                                         >  2:2396237/1‑149 (MQ=255)
                        atatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATg                                                                                                                         >  2:2396242/1‑149 (MQ=255)
                        atatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATg                                                                                                                         >  1:2396245/1‑149 (MQ=255)
                         tatGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACAGCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATgg                                                                                                                        <  1:2396122/149‑1 (MQ=255)
                           tGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGc                                                                                                                      <  2:2396125/149‑1 (MQ=255)
                              aCCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTc                                                                                                                   <  2:2396128/149‑1 (MQ=255)
                                cACGCGGAAGTGTTCAGCGAAAATCAGGACAACGTGGTCGCCACGACCGGACGTCTGCTGCAATACAAAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGc                                                                                                                 >  2:2396301/1‑149 (MQ=255)
                                  cgcgGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTc                                                                                                               <  2:2396130/149‑1 (MQ=255)
                                   gcgGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCa                                                                                                              <  1:2396131/149‑1 (MQ=255)
                                     ggAAGTGTTCAGCGAAAATCAGGACATCTTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAgg                                                                                                            <  1:2396133/149‑1 (MQ=255)
                                      gAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGa                                                                                                           >  1:2396198/1‑149 (MQ=255)
                                       aaGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTAGGCTGGATATGGGCTTCGCTCAGGat                                                                                                          >  2:2396209/1‑149 (MQ=255)
                                         gtgtTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGatat                                                                                                        <  2:2396137/149‑1 (MQ=255)
                                          tgtTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATc                                                                                                       <  2:2396140/149‑1 (MQ=255)
                                            ttCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGa                                                                                                     <  1:2396143/149‑1 (MQ=255)
                                              cAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAAc                                                                                                   <  1:2396146/149‑1 (MQ=255)
                                                gCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACat                                                                                                 >  2:2396218/1‑149 (MQ=255)
                                                  gAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACatat                                                                                               <  1:2396148/149‑1 (MQ=255)
                                                  gAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACatat                                                                                               >  2:2396268/1‑149 (MQ=255)
                                                    aaaTCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGTGCTTCGCTCAGGATATCGAACATATTg                                                                                             >  1:2396260/1‑149 (MQ=255)
                                                     aaTCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGc                                                                                            >  2:2396354/1‑149 (MQ=255)
                                                      aTCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATAtt                                                                                              >  2:2396149/1‑146 (MQ=255)
                                                       tCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTg                                                                                          >  2:2396290/1‑149 (MQ=255)
                                                        cAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTgg                                                                                         <  1:2396152/149‑1 (MQ=255)
                                                         aGGACATCGTGGTGGCCACGACCGGACGTCTTCTGAAATACATAAAAGAAGAGATCTTCGATTGCCGCTCGGTTGAAACTCTGATCCTCAACGATGCAGACCGTATGCTGGATTTGGGCTTCGCTCAGGATATCGAACATATTGCTGGc                                                                                        <  1:2396154/149‑1 (MQ=255)
                                                         aGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGc                                                                                        >  2:2396335/1‑149 (MQ=255)
                                                          ggACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCg                                                                                       >  1:2396204/1‑149 (MQ=255)
                                                                cGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAcgc                                                                                 <  1:2396159/149‑1 (MQ=255)
                                                                 gTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAcgcg                                                                                >  2:2396297/1‑149 (MQ=255)
                                                                  tGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACgcgc                                                                               <  1:2396161/149‑1 (MQ=255)
                                                                    gTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTg                                                                             <  2:2396162/149‑1 (MQ=255)
                                                                         cACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTa                                                                        <  1:2396164/149‑1 (MQ=255)
                                                                          aCGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaa                                                                       >  1:2396226/1‑149 (MQ=255)
                                                                          aCGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaa                                                                       >  2:2396229/1‑149 (MQ=255)
                                                                          aCGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaa                                                                       >  2:2396321/1‑149 (MQ=255)
                                                                             aCCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATTGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACa                                                                    <  2:2396167/149‑1 (MQ=255)
                                                                              ccGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAg                                                                   <  2:2396169/149‑1 (MQ=255)
                                                                                ggACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAc                                                                 <  1:2396170/149‑1 (MQ=255)
                                                                                        gctgcAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGctct                                                         >  2:2396273/1‑149 (MQ=255)
                                                                                        gctgcAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGctct                                                         >  2:2396323/1‑149 (MQ=255)
                                                                                         ctgcAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCtt                                                        >  2:2396279/1‑149 (MQ=255)
                                                                                          tgcAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTGGAAACGCTGATCCTCAACGAAGCAGCCCGTATGCTGGATATGCGCAACGCTCACGATGTCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCatt                                                       >  1:2396174/1‑149 (MQ=255)
                                                                                           gcAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCtttt                                                      <  1:2396177/149‑1 (MQ=255)
                                                                                            cAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTc                                                     <  2:2396180/149‑1 (MQ=255)
                                                                                             aaTACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCg                                                    >  2:2396304/1‑149 (MQ=255)
                                                                                             aaTACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCg                                                    <  1:2396181/149‑1 (MQ=255)
                                                                                                   tAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACg                                              >  1:2396359/1‑149 (MQ=255)
                                                                                                    aaaaGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGc                                             >  2:2396252/1‑149 (MQ=255)
                                                                                                     aaaGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCt                                            <  2:2396186/149‑1 (MQ=255)
                                                                                                      aagaagAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTg                                           <  1:2396187/149‑1 (MQ=255)
                                                                                                       agcagagAACTTCGATTGCCGCGCGGTGGAAACGCTGAGCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCCgg                                          <  2:2396188/146‑1 (MQ=255)
                                                                                                        gaagaGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGa                                         <  1:2396191/149‑1 (MQ=255)
                                                                                                        gaagaGAACTTCGATTGCCGCGCGGGTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCGGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGa                                         <  1:3936551/149‑1 (MQ=255)
                                                                                                         aagaGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGaa                                        <  1:2396195/149‑1 (MQ=255)
                                                                                                         aagaGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGaa                                        <  2:2396193/149‑1 (MQ=255)
                                                                                                          agagAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCGCAACGAAGCAGACCGTATGCGGGATATGGGCTGCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAg                                       <  2:2396196/149‑1 (MQ=255)
                                                                                                           gagaACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAgg                                      >  2:2396330/1‑149 (MQ=255)
                                                                                                             gaACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAgg                                      >  1:2396286/1‑147 (MQ=255)
                                                                                                              aaCTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGgcga                                   <  2:2396198/149‑1 (MQ=255)
                                                                                                                  tCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGgcgatgcg                               >  1:2396337/1‑149 (MQ=255)
                                                                                                                  tCGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGgcgatgcg                               >  1:2396309/1‑149 (MQ=255)
                                                                                                                   cGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTTGGCAACGCTGGAAGgcgatgcga                              >  1:2396223/1‑149 (MQ=255)
                                                                                                                   cGATTGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGgcgatgcga                              <  2:2396202/149‑1 (MQ=255)
                                                                                                                      ttGCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTc                           <  2:2396204/149‑1 (MQ=255)
                                                                                                                        gCCGCGCGGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAg                         >  1:2396303/1‑149 (MQ=255)
                                                                                                                           gcgcgGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGAc                      >  1:2396312/1‑149 (MQ=255)
                                                                                                                             gcgGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACtt                    >  2:2396282/1‑149 (MQ=255)
                                                                                                                              cgGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACttt                   >  1:2396266/1‑149 (MQ=255)
                                                                                                                              cgGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACttt                   <  1:2396209/149‑1 (MQ=255)
                                                                                                                              cgGTTGAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACttt                   <  2:2396211/149‑1 (MQ=255)
                                                                                                                                  tGAAACGCTGATCCTCAAAGACGCAGACCGTATGCGGGATATGGGCTTCGCTCAGGATATCGAACATTTTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCg               <  1:2396212/149‑1 (MQ=255)
                                                                                                                                   gAAACGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGa              >  1:2396371/1‑149 (MQ=255)
                                                                                                                                    aaaCGCTGATCCTCAACGAATCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGATGGCGAAACGCGCTTGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGTCGATGCGATTCAGGACTTTGCCGa              <  1:2396215/148‑1 (MQ=255)
                                                                                                                                    aaaCGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAg             >  2:2396345/1‑149 (MQ=255)
                                                                                                                                     aaCGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGc            <  1:2396217/149‑1 (MQ=255)
                                                                                                                                      aCGCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCg           >  1:2396269/1‑149 (MQ=255)
                                                                                                                                        gCTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTc         >  1:2396306/1‑149 (MQ=255)
                                                                                                                                         cTGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTct        >  1:2396320/1‑149 (MQ=255)
                                                                                                                                          tGATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTctg       <  1:2396220/149‑1 (MQ=255)
                                                                                                                                           gATCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTctgc      >  1:2396349/1‑149 (MQ=255)
                                                                                                                                            aTCCTCAACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTctgct     <  2:2396222/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCACCATCACCGGCGGCGTAGCCTATATGAACCACGCGGAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATACATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGA  >  U00096/2713218‑2713509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑