BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	4,118,444	Δ2,089 bp		zapB–[hslU]	zapB, rraA, menA, [hslU]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	4118444	4120532	2089	288 [0]	[0] 286	zapB–[hslU]	zapB,rraA,menA,[hslU]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 4118443	0 (0.000)	283 (1.130)	158/290	0.2	100%	intergenic (‑353/‑72)	glpF/zapB	glycerol facilitator/cell division factor ZapB
*	?	U00096	4120533 =	0 (0.000)	283 (1.130)	158/290	0.2	100%	coding (1215/1332 nt)	hslU	ATPase component of the HslVU protease

Only 100 of 286 total aligned reads displayed.

ATCAAACAATGATGAAAATGATTGAACGCGATTATAGGATAAAACGGCTCAGATCTTCATCTGCCACCAACGCATCCAGATGTTTGCTCACATAATCTGCGTCAATAGTGATATTTTGACCGCTTAAATCGCTGGCGTCGTAGGAAATCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTGCTT  >  U00096/4120385‑4120681

cgcaacaaatttattaacattttcgattgatgtggattctgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtc                                                                                                                                                      <  2:3581037‑M2/1‑1 (MQ=255)

 gcaacaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtct                                                                                                                                                     >  2:3581169‑M2/148‑149 (MQ=255)

  caacaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtctc                                                                                                                                                    <  1:3581038‑M2/3‑1 (MQ=255)

   aacaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtctct                                                                                                                                                   >  2:3581226‑M2/146‑149 (MQ=255)

       aatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCa                                                                                                                                               >  1:3581184‑M2/142‑149 (MQ=255)

        atttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCAt                                                                                                                                              <  2:3581042‑M2/9‑1 (MQ=255)

         tttattaacattttcgattgatgtggattatgtcaccaggagaaggatgcaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCAtt                                                                                                                                             >  2:3581252‑M2/140‑149 (MQ=255)

         tttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCAtt                                                                                                                                             >  1:3581219‑M2/140‑149 (MQ=255)

           tattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTaa                                                                                                                                           >  2:3581238‑M2/138‑149 (MQ=255)

            attaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccttaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTaaa                                                                                                                                          >  1:3581201‑M2/137‑149 (MQ=255)

            attaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTaaa                                                                                                                                          <  2:3581045‑M2/13‑1 (MQ=255)

             ttaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAAc                                                                                                                                         <  1:3581046‑M2/14‑1 (MQ=255)

               aacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGc                                                                                                                                       >  2:3581142‑M2/134‑149 (MQ=255)

                   ttttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCa                                                                                                                                   >  2:3581223‑M2/130‑149 (MQ=255)

                   ttttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCa                                                                                                                                   >  1:3581180‑M2/130‑149 (MQ=255)

                    tttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAg                                                                                                                                  >  1:3581298‑M2/129‑149 (MQ=255)

                     ttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACg                                                                                                                                        >  1:3581292‑M2/128‑142 (MQ=255)

                     ttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGa                                                                                                                                 <  2:3581049‑M2/22‑1 (MQ=255)

                      tcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGaa                                                                                                                                >  1:3581281‑M2/127‑149 (MQ=255)

                      tcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGaa                                                                                                                                >  1:3581276‑M2/127‑149 (MQ=255)

                      tcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGaa                                                                                                                                <  1:3581052‑M2/23‑1 (MQ=255)

                       cgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAAc                                                                                                                               >  1:3581272‑M2/126‑149 (MQ=255)

                        gattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACa                                                                                                                              >  1:3581284‑M2/125‑149 (MQ=255)

                        gattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACa                                                                                                                              <  2:3581055‑M2/25‑1 (MQ=255)

                           tgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtg                                                                                                                           >  2:3581139‑M2/122‑149 (MQ=255)

                            gatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgt                                                                                                                          >  1:3581124‑M2/121‑149 (MQ=255)

                                tggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTaa                                                                                                                      >  1:3581195‑M2/117‑149 (MQ=255)

                                tggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTaa                                                                                                                      >  2:3581178‑M2/117‑149 (MQ=255)

                                 ggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTaaa                                                                                                                     <  1:3581060‑M2/34‑1 (MQ=255)

                                  gattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAac                                                                                                                    <  2:3581061‑M2/35‑1 (MQ=255)

                                   attatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacg                                                                                                                   <  2:3581065‑M2/36‑1 (MQ=255)

                                    ttatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgtgt                                                                                                                        >  1:3581249‑M2/113‑143 (MQ=255)

                                    ttatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacga                                                                                                                  <  1:3581070‑M2/37‑1 (MQ=255)

                                    ttatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacga                                                                                                                  <  1:3581066‑M2/37‑1 (MQ=255)

                                     tatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacgac                                                                                                                 >  1:3581232‑M2/112‑149 (MQ=255)

                                      atgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacgacg                                                                                                                <  2:3581072‑M2/39‑1 (MQ=255)

                                              aggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGa                                                                                                        >  2:3581287‑M2/103‑149 (MQ=255)

                                               ggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGAt                                                                                                       <  2:3581080‑M2/48‑1 (MQ=255)

                                               ggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGAt                                                                                                       <  1:3581081‑M2/48‑1 (MQ=255)

                                               ggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGAt                                                                                                       >  2:3581151‑M2/102‑149 (MQ=255)

                                                   aaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGttt                                                                                                   >  1:3581189‑M2/98‑149 (MQ=255)

                                                       atgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCgg                                                                                               <  1:3581089‑M2/56‑1 (MQ=255)

                                                        tgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGt                                                                                              <  2:3581092‑M2/57‑1 (MQ=255)

                                                        tgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAATGTGTAAACGACGAGCACCGATGTTTTCGGt                                                                                              >  2:3581128‑M2/93‑149 (MQ=255)

                                                         gaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTa                                                                                             <  1:3581093‑M2/58‑1 (MQ=255)

                                                             agtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGAtt                                                                                         >  2:3581208‑M2/88‑149 (MQ=255)

                                                              gtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGAtt                                                                                         <  1:3581097‑M2/62‑1 (MQ=255)

                                                                  ggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTc                                                                                    <  2:3581103‑M2/67‑1 (MQ=255)

                                                                  ggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTc                                                                                    <  1:3581102‑M2/67‑1 (MQ=255)

                                                                   gcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCa                                                                                   >  2:3581190‑M2/82‑149 (MQ=255)

                                                                      aaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCt                                                                                <  1:3581108‑M2/71‑1 (MQ=255)

                                                                       accgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTACGGTAGATTCGTTCa                                                                                   >  1:3581304‑M2/78‑145 (MQ=255)

                                                                        ccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTg                                                                               >  1:3581318‑M2/77‑148 (MQ=255)

                                                                        ccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGc                                                                              >  2:3581277‑M2/77‑149 (MQ=255)

                                                                        ccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGc                                                                              >  1:3581209‑M2/77‑149 (MQ=255)

                                                                                tgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGc                                                                      >  2:3581323‑M2/69‑149 (MQ=255)

                                                                                tgcaaggcgtgccgctggacagcgatagcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTGCCATTACACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGc                                                                      >  1:3581120‑M2/69‑149 (MQ=255)

                                                                                 gcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGc                                                                         <  2:3581153‑M2/78‑1 (MQ=255)

                                                                                         ttccgatggacagcgatggcgcggcttcattcaatcggagctaactaaagtgcgctcgtCTCTTCCATTATACGCTCCAGTAGTGTGTTTAAACGTCGAGCACAGATGTTTTCGTTAGATTCGTTCACCTGCCATGCCGCTGCCGCGAt                                                             <  2:3581112‑M2/90‑1 (MQ=255)

                                                                                         tgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGAt                                                             >  2:3581202‑M2/60‑149 (MQ=255)

                                                                                         tgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGAt                                                             >  2:3581380‑M2/60‑149 (MQ=255)

                                                                                             gctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGt                                                         >  1:3581242‑M2/56‑149 (MQ=255)

                                                                                                ggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTa                                                      >  2:3581217‑M2/53‑149 (MQ=255)

                                                                                                    agcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATAc                                                  <  2:3581160‑M2/101‑1 (MQ=255)

                                                                                                      cgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCg                                                >  2:3581367‑M2/47‑149 (MQ=255)

                                                                                                       gatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCgg                                               <  1:3581168‑M2/104‑1 (MQ=255)

                                                                                                       gatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCgg                                               <  1:3581155‑M2/104‑1 (MQ=255)

                                                                                                          ggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCGAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGt                                            <  2:3581156‑M2/107‑1 (MQ=255)

                                                                                                          ggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGt                                            <  1:3581135‑M2/107‑1 (MQ=255)

                                                                                                          ggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGt                                            <  2:3581166‑M2/107‑1 (MQ=255)

                                                                                                              cggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGAGGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGt                                        <  2:3581145‑M2/111‑1 (MQ=255)

                                                                                                               ggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTTAACGCTCCAGAACAGTATGTAAACGACGAGCACCGAGGTTTTCGGAAGATTCGATCACCTGCCATCCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTa                                       >  2:3581119‑M2/38‑149 (MQ=255)

                                                                                                                  ttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAAc                                    <  1:3581172‑M2/115‑1 (MQ=255)

                                                                                                                   tcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACt                                   <  1:3581175‑M2/116‑1 (MQ=255)

                                                                                                                     atacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCg                                 >  1:3581236‑M2/32‑149 (MQ=255)

                                                                                                                      tacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGa                                <  2:3581134‑M2/119‑1 (MQ=255)

                                                                                                                      tacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGa                                >  1:3581259‑M2/31‑149 (MQ=255)

                                                                                                                          atcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTa                                       <  1:3581162‑M2/112‑1 (MQ=255)

                                                                                                                          atcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATAtt                            >  2:3581265‑M2/27‑149 (MQ=255)

                                                                                                                            cggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTa                          <  2:3581180‑M2/125‑1 (MQ=255)

                                                                                                                             ggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTAc                         <  2:3581125‑M2/126‑1 (MQ=255)

                                                                                                                             ggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTAc                         >  1:3581398‑M2/24‑149 (MQ=255)

                                                                                                                              gagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACg                        >  2:3581258‑M2/23‑149 (MQ=255)

                                                                                                                               agctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGc                       <  1:3581132‑M2/128‑1 (MQ=255)

                                                                                                                               agctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGc                       <  1:3581142‑M2/128‑1 (MQ=255)

                                                                                                                                 ctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCt                     >  1:3581264‑M2/20‑149 (MQ=255)

                                                                                                                                     ctaaagtgcgctcgtCTCTTCCATTACACGCGCCAGAACAGAGAGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAAAACCGGAGTCGGTAAACTCGATATTTACGCCTACAg                 >  2:3581340‑M2/16‑149 (MQ=255)

                                                                                                                                     ctaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAg                 <  1:3581151‑M2/134‑1 (MQ=255)

                                                                                                                                      taaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGt                >  2:3581246‑M2/15‑149 (MQ=255)

                                                                                                                                          gtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGcc            >  1:3581374‑M2/11‑149 (MQ=255)

                                                                                                                                          gtgcgctcgtCTCGTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGCTGTTTTCGGGAGATTCGTTCACCTGCCATGCCGCTTCCGCGCTGCGTTTAATACCGGAGTCGGTAAACTCGATATTTCCGCCTTCAGTCGcc            >  2:3581301‑M2/11‑149 (MQ=255)

                                                                                                                                           tgcgctcttCTCTTCCATTAAACGCTCCAGAACAGTGTGTATACGACGTGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCa           <  1:3581148‑M2/140‑1 (MQ=255)

                                                                                                                                           tgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCa           <  1:3581139‑M2/140‑1 (MQ=255)

                                                                                                                                            gcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCAt          >  1:3581349‑M2/9‑149 (MQ=255)

                                                                                                                                             cgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATc         >  2:3581316‑M2/8‑149 (MQ=255)

                                                                                                                                                tcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGt      <  2:3581184‑M2/145‑1 (MQ=255)

                                                                                                                                                 cgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTg     <  2:3581173‑M2/146‑1 (MQ=255)

                                                                                                                                                 cgtCTCTTCCATTAAACGCTCCAGAACAATGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGt      <  1:3581128‑M2/145‑1 (MQ=255)

                                                                                                                                                  gtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTa                          <  1:3581115‑M2/125‑1 (MQ=255)

                                                                                                                                                   tCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTGCt   >  1:3581328‑M2/2‑149 (MQ=255)

ATCAAACAATGATGAAAATGATTGAACGCGATTATAGGATAAAACGGCTCAGATCTTCATCTGCCACCAACGCATCCAGATGTTTGCTCACATAATCTGCGTCAATAGTGATATTTTGACCGCTTAAATCGCTGGCGTCGTAGGAAATCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTGCTT  >  U00096/4120385‑4120681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`