BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	4,118,444	Δ2,089 bp		zapB–[hslU]	zapB, rraA, menA, [hslU]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	4118444	4120532	2089	225 [1]	[0] 224	zapB–[hslU]	zapB,rraA,menA,[hslU]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 4118443	1 (0.010)	221 (1.200)	144/290	0.1	99.8%	intergenic (‑353/‑72)	glpF/zapB	glycerol facilitator/cell division factor ZapB
*	?	U00096	4120533 =	0 (0.000)	221 (1.200)	144/290	0.1	99.8%	coding (1215/1332 nt)	hslU	ATPase component of the HslVU protease

Only 100 of 224 total aligned reads displayed.

ATCAAACAATGATGAAAATGATTGAACGCGATTATAGGATAAAACGGCTCAGATCTTCATCTGCCACCAACGCATCCAGATGTTTGCTCACATAATCTGCGTCAATAGTGATATTTTGACCGCTTAAATCGCTGGCGTCGTAGGAAATCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTGCTT  >  U00096/4120385‑4120681

cgcaacaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtc                                                                                                                                                      <  1:2647370‑M2/1‑1 (MQ=255)

 gcaacaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtct                                                                                                                                                     <  2:2647373‑M2/2‑1 (MQ=255)

   aacaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtctct                                                                                                                                                   >  2:2647460‑M2/146‑149 (MQ=255)

    acaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCtt                                                                                                                                                  >  2:2647500‑M2/145‑149 (MQ=255)

    acaaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCtt                                                                                                                                                  >  2:2647474‑M2/145‑149 (MQ=255)

     caaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTc                                                                                                                                                 <  2:2647376‑M2/6‑1 (MQ=255)

      aaatttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTcc                                                                                                                                                <  1:2647377‑M2/7‑1 (MQ=255)

        atttattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCAt                                                                                                                                              >  1:2647499‑M2/141‑149 (MQ=255)

           tattaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTaa                                                                                                                                           <  1:2647379‑M2/12‑1 (MQ=255)

            attaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTaaa                                                                                                                                          >  2:2647519‑M2/137‑149 (MQ=255)

             ttaacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAAc                                                                                                                                         >  1:2647548‑M2/136‑149 (MQ=255)

              taacattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAAc                                                                                                                                         <  1:2647383‑M2/14‑1 (MQ=255)

                acattttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCt                                                                                                                                      <  1:2647385‑M2/17‑1 (MQ=255)

                   ttttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcagcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCa                                                                                                                                   >  2:2647469‑M2/130‑149 (MQ=255)

                    tttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcacaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAg                                                                                                                                  >  2:2647450‑M2/129‑149 (MQ=255)

                     ttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCtca                                                                                                                                    >  2:2647550‑M2/128‑145 (MQ=255)

                     ttcgattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTcc                                                                                                                                    >  1:2647475‑M2/128‑146 (MQ=255)

                        gattgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACa                                                                                                                              >  1:2647481‑M2/125‑149 (MQ=255)

                         attgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAg                                                                                                                             <  2:2647387‑M2/26‑1 (MQ=255)

                         attgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAg                                                                                                                             >  1:2647496‑M2/124‑149 (MQ=255)

                         attgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAg                                                                                                                             >  1:2647493‑M2/124‑149 (MQ=255)

                         attgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAg                                                                                                                             >  1:2647472‑M2/124‑149 (MQ=255)

                          ttgatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgt                                                                                                                            >  1:2647445‑M2/123‑149 (MQ=255)

                           tgatgtggattatgtcaccatgagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtg                                                                                                                           <  2:2647389‑M2/28‑1 (MQ=255)

                            gatgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgt                                                                                                                          <  2:2647391‑M2/29‑1 (MQ=255)

                             atgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgtg                                                                                                                         <  1:2647394‑M2/30‑1 (MQ=255)

                              tgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgtgt                                                                                                                        >  2:2647446‑M2/119‑149 (MQ=255)

                              tgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgtgt                                                                                                                        >  2:2647565‑M2/119‑149 (MQ=255)

                              tgtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAgtgtgt                                                                                                                        >  1:2647511‑M2/119‑149 (MQ=255)

                               gtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTa                                                                                                                       <  2:2647397‑M2/32‑1 (MQ=255)

                               gtggattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTa                                                                                                                       <  2:2647395‑M2/32‑1 (MQ=255)

                                  gattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAac                                                                                                                    <  2:2647400‑M2/35‑1 (MQ=255)

                                  gattatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAac                                                                                                                    >  2:2647526‑M2/115‑149 (MQ=255)

                                     tatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacgac                                                                                                                 <  1:2647404‑M2/38‑1 (MQ=255)

                                     tatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacgac                                                                                                                 <  2:2647403‑M2/38‑1 (MQ=255)

                                     tatgtcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAacgac                                                                                                                 >  1:2647491‑M2/112‑149 (MQ=255)

                                         tcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGc                                                                                                             <  2:2647408‑M2/42‑1 (MQ=255)

                                         tcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGc                                                                                                             >  1:2647411‑M2/108‑149 (MQ=255)

                                         tcaccaggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGc                                                                                                             <  1:2647407‑M2/42‑1 (MQ=255)

                                              aggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGa                                                                                                        <  2:2647411‑M2/47‑1 (MQ=255)

                                               ggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGAt                                                                                                       >  1:2647455‑M2/102‑149 (MQ=255)

                                               ggagaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGAt                                                                                                       >  2:2647479‑M2/102‑149 (MQ=255)

                                                  gaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGtt                                                                                                    <  2:2647414‑M2/51‑1 (MQ=255)

                                                  gaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGtt                                                                                                    >  1:2647531‑M2/99‑149 (MQ=255)

                                                  gaaggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGtt                                                                                                    >  2:2647529‑M2/99‑149 (MQ=255)

                                                     ggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTc                                                                                                 <  1:2647416‑M2/54‑1 (MQ=255)

                                                     ggatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTc                                                                                                 >  2:2647540‑M2/96‑149 (MQ=255)

                                                      gatgaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCg                                                                                                <  2:2647418‑M2/55‑1 (MQ=255)

                                                         gaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTa                                                                                             <  2:2647423‑M2/58‑1 (MQ=255)

                                                         gaaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgcgcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTa                                                                                             <  2:2647421‑M2/58‑1 (MQ=255)

                                                          aaaagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAg                                                                                            <  1:2647424‑M2/59‑1 (MQ=255)

                                                            aagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGACCAGTGTGTAAACGACGAGCACCGATGTTTTCGGt                                                                                              >  1:2647580‑M2/89‑145 (MQ=255)

                                                            aagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGt                                                                                              >  2:2647507‑M2/89‑145 (MQ=255)

                                                            aagtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGAt                                                                                          <  2:2647428‑M2/61‑1 (MQ=255)

                                                             cgggtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGAtt                                                                                         <  2:2647433‑M2/62‑1 (MQ=255)

                                                             agtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGAtt                                                                                         <  1:2647429‑M2/62‑1 (MQ=255)

                                                              gtgtggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTc                                                                                        <  2:2647430‑M2/63‑1 (MQ=255)

                                                                  ggcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCAACAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTc                                                                                    >  2:2647583‑M2/83‑149 (MQ=255)

                                                                   gcaaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGtt                                                                                     <  1:2647434‑M2/66‑1 (MQ=255)

                                                                     aaaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCAcc                                                                                 >  2:2647513‑M2/80‑149 (MQ=255)

                                                                      aaccgtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCt                                                                                <  2:2647436‑M2/71‑1 (MQ=255)

                                                                          gtaatctgcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCa                                                                            >  2:2647516‑M2/75‑149 (MQ=255)

                                                                                 gcaaggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGcc                                                                        <  2:2647484‑M2/79‑1 (MQ=255)

                                                                                     ggcgtgccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGc                                                                      >  1:2647522‑M2/64‑144 (MQ=255)

                                                                                          gccgctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATg                                                            <  2:2647453‑M2/91‑1 (MQ=255)

                                                                                              ctggacagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGtt                                                        >  1:2647505‑M2/55‑149 (MQ=255)

                                                                                                  acagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAAt                                                    <  1:2647488‑M2/99‑1 (MQ=255)

                                                                                                  acagcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAAt                                                    <  1:2647461‑M2/99‑1 (MQ=255)

                                                                                                    agcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATAc                                                  <  2:2647465‑M2/101‑1 (MQ=255)

                                                                                                     gcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAACCGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCATCCGCGATGCGTTTAATAcc                                                 <  2:2647442‑M2/102‑1 (MQ=255)

                                                                                                     gcgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATAcc                                                 <  2:2647481‑M2/102‑1 (MQ=255)

                                                                                                     gcgatggcgcagcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATAcc                                                 <  1:2647469‑M2/102‑1 (MQ=255)

                                                                                                      cgatggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCg                                                <  2:2647472‑M2/103‑1 (MQ=255)

                                                                                                        atggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGa                                              >  2:2647590‑M2/45‑149 (MQ=255)

                                                                                                          ggcgcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGt                                            >  2:2647535‑M2/43‑149 (MQ=255)

                                                                                                             gcggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCgg                                         >  1:2647574‑M2/40‑149 (MQ=255)

                                                                                                              cggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGt                                        >  1:2647629‑M2/39‑149 (MQ=255)

                                                                                                               ggcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAAGGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTa                                       <  1:2647450‑M2/112‑1 (MQ=255)

                                                                                                                gcttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTaa                                      <  1:2647486‑M2/113‑1 (MQ=255)

                                                                                                                 cttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCg                                          <  1:2647466‑M2/109‑1 (MQ=255)

                                                                                                                 cttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTaaa                                     >  1:2647555‑M2/36‑149 (MQ=255)

                                                                                                                  ttcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAAc                                    >  2:2647440‑M2/35‑149 (MQ=255)

                                                                                                                   tcatacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACt                                   >  1:2647560‑M2/34‑149 (MQ=255)

                                                                                                                    catacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTc                                  <  1:2647459‑M2/117‑1 (MQ=255)

                                                                                                                      tacaatcggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGa                                <  1:2647441‑M2/119‑1 (MQ=255)

                                                                                                                            cggagctaactaaagtgcgctcgtCTCTTCCATTAACCGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCCTGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTa                          >  2:2647559‑M2/25‑149 (MQ=255)

                                                                                                                            cggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTa                          <  1:2647464‑M2/125‑1 (MQ=255)

                                                                                                                             ggagctaactaaagtgcgctcgtCTCTTCCATTACACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTAc                         <  1:2647448‑M2/126‑1 (MQ=255)

                                                                                                                             ggagctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTAc                         <  1:2647483‑M2/126‑1 (MQ=255)

                                                                                                                               agctaactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGc                       <  2:2647444‑M2/128‑1 (MQ=255)

                                                                                                                                  taactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGa                                                                                                        <  1:2647457‑M2/47‑1 (MQ=255)

                                                                                                                                  taactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCtt                    >  2:2647553‑M2/19‑149 (MQ=255)

                                                                                                                                   aactaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTc                   <  2:2647477‑M2/132‑1 (MQ=255)

                                                                                                                                    actaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCa                  <  2:2647455‑M2/133‑1 (MQ=255)

                                                                                                                                    actaaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCa                  >  1:2647620‑M2/17‑149 (MQ=255)

                                                                                                                                     actaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAg                 >  1:2647611‑M2/16‑149 (MQ=255)

                                                                                                                                       aaagtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTc               >  2:2647545‑M2/14‑149 (MQ=255)

                                                                                                                                          gtgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGcc            >  1:2647564‑M2/11‑149 (MQ=255)

                                                                                                                                           tgcgctcgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCa           <  2:2647467‑M2/140‑1 (MQ=255)

                                                                                                                                                 cgtCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTg     >  1:2647595‑M2/4‑149 (MQ=255)

ATCAAACAATGATGAAAATGATTGAACGCGATTATAGGATAAAACGGCTCAGATCTTCATCTGCCACCAACGCATCCAGATGTTTGCTCACATAATCTGCGTCAATAGTGATATTTTGACCGCTTAAATCGCTGGCGTCGTAGGAAATCTCTTCCATTAAACGCTCCAGAACAGTGTGTAAACGACGAGCACCGATGTTTTCGGTAGATTCGTTCACCTGCCATGCCGCTTCCGCGATGCGTTTAATACCGGAGTCGGTAAACTCGATATTTACGCCTTCAGTCGCCATCAGTGCTT  >  U00096/4120385‑4120681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`