Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG23.5% 230.2 / 19.5 121intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (19/72);  new base G (27/1);  total (48/73)
Fisher's exact test for biased strand distribution p-value = 2.26e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 129 total aligned reads displayed.

TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  U00096/422281‑422577
                                                                                                                                                    |                                                                                                                                                    
tttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGt                                                                                                                                                      <  1:256800/149‑1 (MQ=255)
     cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                 <  2:256801/149‑1 (MQ=255)
      gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtccacct                                                                                                                                                >  2:256920/1‑149 (MQ=255)
            tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCa                                                                                                                                          <  2:256802/149‑1 (MQ=255)
              ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                        <  1:1120194/149‑1 (MQ=255)
               tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                       <  1:256804/149‑1 (MQ=255)
                gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCTCCGCCCCCAAatc                                                                                                                                      >  2:256880/1‑148 (MQ=255)
                gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                      <  2:256805/149‑1 (MQ=255)
                 tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTGCGCCCCCACatat                                                                                                                                     >  2:256908/1‑149 (MQ=255)
                    tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGTGCTCCCCCAAaaatacg                                                                                                                                  >  2:256834/1‑146 (MQ=255)
                     gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGGCGGCCGCCGCCCCCACAGATCCtg                                                                                                                                 >  2:256901/1‑148 (MQ=255)
                             gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                         <  1:256807/149‑1 (MQ=255)
                               tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTCCGCCACCAAATCTCCTGCAgatgatta                                                                                                                       >  2:256874/1‑147 (MQ=255)
                               tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                       <  2:256808/149‑1 (MQ=255)
                               tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAGAATCCGTAAGAtgatga                                                                                                                       >  2:256875/1‑149 (MQ=255)
                               tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCGCCGAATAGCCTGCAGAtgagga                                                                                                                       >  2:256865/1‑149 (MQ=255)
                                 gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                     >  2:256949/1‑149 (MQ=255)
                                   tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                   <  2:256809/149‑1 (MQ=255)
                                     tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGAGGATGAGTGATc                                                                                                                 >  2:256919/1‑149 (MQ=255)
                                      ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCTGCTCCCCCAAAGATCCATCAGATGAGCAGTgttgc                                                                                                                >  2:256975/1‑145 (MQ=255)
                                         aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                             <  2:256810/149‑1 (MQ=255)
                                              cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTCCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                        <  1:256811/149‑1 (MQ=255)
                                               gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGCATTAgg                                                                                                       >  2:256929/1‑149 (MQ=255)
                                                ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGTCCACCTCACCCAAATAACCATCAGATGAAGAGAGATACTGCATAAgga                                                                                                      >  2:256938/1‑148 (MQ=255)
                                                  aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATCCTTCAGATGAGGAGTGATCCTGGATTAgggga                                                                                                    >  2:256872/1‑146 (MQ=255)
                                                  aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCGGCATTAGGCTa                                                                                                    >  2:256914/1‑149 (MQ=255)
                                                    gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                    <  1:256813/147‑1 (MQ=255)
                                                    gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCACCTCCCCCAAAAAAACTTCATAATATGATAGATACTGCATAATGCTATg                                                                                                  >  2:256863/1‑149 (MQ=255)
                                                        gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                              <  1:2278032/149‑1 (MQ=255)
                                                        gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAAAAGCATGCAGATGATGAGTGATCGTGCATTAGGCTATGGCaa                                                                                              >  2:256926/1‑149 (MQ=255)
                                                          cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                            <  2:256814/149‑1 (MQ=255)
                                                           aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                           >  2:256948/1‑149 (MQ=255)
                                                            gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                          <  1:256816/149‑1 (MQ=255)
                                                             ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGa                                                                                         <  2:256817/149‑1 (MQ=255)
                                                               ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                        <  1:256818/132‑1 (MQ=255)
                                                                 ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                     <  2:256819/149‑1 (MQ=255)
                                                                   gAAAACCATTAATCCATGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                  <  1:256820/86‑1 (MQ=255)
                                                                   gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                   <  2:256822/149‑1 (MQ=255)
                                                                   gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCACCGTCCACCGCACCCAAATATCATTCAGATGATGAGTGATACTGCATTAGGGTATGGCATGGTGATCaaaa                                                                                   >  2:256841/1‑146 (MQ=255)
                                                                     aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCTCCCCCCAATATCCTTGAGATGATGAGTGGTGCTGCAGGAGGCGATGGGAAGGTGGTGAGAttt                                                                                 >  2:256846/1‑149 (MQ=255)
                                                                         cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                              <  1:256823/148‑1 (MQ=255)
                                                                          aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                            <  1:256824/149‑1 (MQ=255)
                                                                          aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTCCGCCCCCACATATACATCAGATGGTGAGTGAGCCTGCATTAGGCTATGGCGAGGGGATAAGATTTtcatc                                                                            >  2:256921/1‑149 (MQ=255)
                                                                            tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGATATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                          <  1:256825/149‑1 (MQ=255)
                                                                            tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCGATTCCGAAAGTTAGCCGCCCCCGGCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATcac                                                                          <  2:256827/149‑1 (MQ=255)
                                                                             aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGGCGCATCGCCCAGCTAACAATCAGAGGATGAGTGATACTGCATTAGGGTATGGCAAGGTGATCGGAttttagtc                                                                            >  2:256923/1‑142 (MQ=255)
                                                                                ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                      <  1:256829/149‑1 (MQ=255)
                                                                                 cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAgggg                                                                     <  2:256831/149‑1 (MQ=255)
                                                                                 cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                     <  1:256830/149‑1 (MQ=255)
                                                                                  ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                    <  1:256832/149‑1 (MQ=255)
                                                                                   tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                   <  1:256834/149‑1 (MQ=255)
                                                                                    gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGGCCCCAAATATAATTCAGATGATGAGTGATCCTGCATTAGTCGATGGCAAGGGGATCAGATTTTCATCAAAGGGGAAt                                                                  >  2:256892/1‑149 (MQ=255)
                                                                                       ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                               <  2:256836/149‑1 (MQ=255)
                                                                                          tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                            <  1:256838/149‑1 (MQ=255)
                                                                                          tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                            <  1:256837/149‑1 (MQ=255)
                                                                                               gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                       <  2:256840/149‑1 (MQ=255)
                                                                                                 cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATACTGCATTAGGCTAGGGCAAGGTGGTCAGATTTTGATCACAGGGGAATTATGATGTTAAAt                                                     >  2:256950/1‑149 (MQ=255)
                                                                                                    ttCATGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                   <  1:256841/148‑1 (MQ=255)
                                                                                                     tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                 <  2:256842/149‑1 (MQ=255)
                                                                                                           ggaAATCCTGATACGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCACCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAca                                           <  2:256845/149‑2 (MQ=255)
                                                                                                            gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                          <  1:256846/149‑1 (MQ=255)
                                                                                                               aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                       <  2:256847/149‑1 (MQ=255)
                                                                                                                tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTGCAGAGGATGAGTGGTCCTGCCGTAGGCTATGGGAAGGTGATCAGCTTTTATTGACAGGGGAATTATGATGTTAATTGCATGGCACCTGCCg                                      >  2:256971/1‑149 (MQ=255)
                                                                                                                 ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAATGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                     <  1:256848/149‑1 (MQ=255)
                                                                                                                  cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                    <  2:256849/149‑1 (MQ=255)
                                                                                                                  cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                    <  1:256850/149‑1 (MQ=255)
                                                                                                                     aTCCGGCTATTCCGAAAGTTATCCGGCGCCGGGGGCCGCCCCCCAAAATCCGTCAGATGATGAGTGATCCAGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAAAAGGGGAATTATGATGTTAAATTAATGGAACCTGCCGGTGcc                                 >  2:256951/1‑149 (MQ=255)
                                                                                                                       ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc                               <  2:256851/149‑1 (MQ=255)
                                                                                                                       ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGGGTGGTCCGGCATTAGGCTATGGCAAGGGGATCAGAGTTTAAGAACAGGGGGATTATGATTTTAAATGCATGGCACCTGCCGGGGcccc                               >  2:257008/1‑149 (MQ=255)
                                                                                                                          gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                            <  2:256853/149‑1 (MQ=255)
                                                                                                                          gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                            <  2:256852/149‑1 (MQ=255)
                                                                                                                            tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGCTGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                          <  1:256854/149‑1 (MQ=255)
                                                                                                                             aTTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                         <  2:256855/149‑1 (MQ=255)
                                                                                                                               tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                       <  2:256857/149‑1 (MQ=255)
                                                                                                                                ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                      <  2:256859/149‑1 (MQ=255)
                                                                                                                                ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                      <  2:256858/149‑1 (MQ=255)
                                                                                                                                 cGAAAGTTATCCGCGCCCGGCCTCCGCCCCCAAATATACTAAAGATGATGAGTGATGATGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATTAATGGCACCGGCCGCTGCCCCCCATTGttta                     >  2:256928/1‑147 (MQ=255)
                                                                                                                                    aaGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTAAAGATGATGAGGGATGCTGCATTTGGGGATGGAAAGGTGATCAGATTTTGATGACAGGGGATTTATGATGTTAAATGAATGGAACCGGCCGGTGCCCCCATTTGTTaaaaa                  >  2:256912/1‑147 (MQ=255)
                                                                                                                                     aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                 <  2:256862/149‑1 (MQ=255)
                                                                                                                                       ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag               <  1:256863/149‑1 (MQ=255)
                                                                                                                                       ttATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATGAGATTTTAATAACAGGGGAATTATTATGTTAAATTAATGGCACCAGCCGGTGCCCCCCGTTGTTAAAcaaag               >  2:256964/1‑149 (MQ=255)
                                                                                                                                        tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc              <  1:256865/149‑1 (MQ=255)
                                                                                                                                        tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGATAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag               <  1:256864/148‑1 (MQ=255)
                                                                                                                                        tATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                        >  1:256915/1‑75 (MQ=255)
                                                                                                                                           ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa           <  2:256868/149‑1 (MQ=255)
                                                                                                                                            cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag          <  2:256869/149‑1 (MQ=255)
                                                                                                                                            cGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTTTGGGAAGGTGATCAGATTTTCATCAAAGGGGAATTATGATGTTAAATGCATGGCACCGGCCGGTGCCCCCATTTGTTAAAcaaagcaaag          >  2:257011/1‑149 (MQ=255)
                                                                                                                                             gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa         <  2:256870/149‑1 (MQ=255)
                                                                                                                                             gCCCCCGAACTCCACCCCCAAATATACTACAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATAAGATTTTAAAAACAGGGGAATTATGATGTTTAATGCATTGCACCAGCCGGTGCCCCCATTTGTTAAAcaaagcaa            >  2:256935/1‑146 (MQ=255)
                                                                                                                                              cccccGTCCTCCTCCCCCAAATATCCATCAGATGATGAGTGATCCTGCATTAGGCTGTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            >  1:256997/1‑145 (MQ=255)
                                                                                                                                               ccccGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt        <  1:256873/148‑1 (MQ=255)
                                                                                                                                               ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa         <  1:256872/147‑1 (MQ=255)
                                                                                                                                                cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc       <  1:256874/148‑1 (MQ=255)
                                                                                                                                                cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa      >  2:256934/1‑149 (MQ=255)
                                                                                                                                                cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa      >  1:256933/1‑149 (MQ=255)
                                                                                                                                                cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa      <  1:1601292/149‑1 (MQ=255)
                                                                                                                                                  cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc    <  1:256875/149‑1 (MQ=255)
                                                                                                                                                    tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  >  2:256913/1‑149 (MQ=255)
                                                                                                                                                    tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  >  2:256905/1‑149 (MQ=255)
                                                                                                                                                    tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  >  2:256987/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  U00096/422281‑422577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑