Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4350TG21.0% 256.3 / 11.2 121intergenic (+76/‑83)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (21/73);  new base G (25/0);  total (48/73)
Fisher's exact test for biased strand distribution p-value = 2.10e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 136 total aligned reads displayed.

GTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  U00096/422287‑422583
                                                                                                                                                    |                                                                                                                                                    
     ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                                 >  2:256850/1‑149 (MQ=255)
     ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGccccc                                                                                                                                                 >  2:256906/1‑149 (MQ=255)
        ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                              <  1:1120194/149‑1 (MQ=255)
        ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                              <  1:256803/149‑1 (MQ=255)
         tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                             <  1:256804/149‑1 (MQ=255)
          gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCTCCGCCCCCAAatc                                                                                                                                            >  2:256880/1‑148 (MQ=255)
          gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                            <  2:256805/149‑1 (MQ=255)
          gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                            >  1:2694837/1‑149 (MQ=255)
           tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTGCGCCCCCACatat                                                                                                                                           >  2:256908/1‑149 (MQ=255)
              tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGTGCTCCCCCAAaaatacg                                                                                                                                        >  2:256834/1‑146 (MQ=255)
               gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGGCGGCCGCCGCCCCCACAGATCCtg                                                                                                                                       >  2:256901/1‑148 (MQ=255)
                ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTc                                                                                                                                      <  2:256806/149‑1 (MQ=255)
                  gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAg                                                                                                                                    >  2:256931/1‑149 (MQ=255)
                       gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                               <  1:256807/149‑1 (MQ=255)
                         tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCTCCGCCACCAAATCTCCTGCAgatgatta                                                                                                                             >  2:256874/1‑147 (MQ=255)
                           gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGt                                                                                                                           >  2:256949/1‑149 (MQ=255)
                             tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGa                                                                                                                         <  2:256809/149‑1 (MQ=255)
                               tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGAGGATGAGTGATc                                                                                                                       >  2:256919/1‑149 (MQ=255)
                                   aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                                   <  2:256810/149‑1 (MQ=255)
                                         gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGCATTAgg                                                                                                             >  2:256929/1‑149 (MQ=255)
                                          ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                            <  1:256812/149‑1 (MQ=255)
                                          ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGTCCACCTCACCCAAATAACCATCAGATGAAGAGAGATACTGCATAAgga                                                                                                            >  2:256938/1‑148 (MQ=255)
                                           cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGGCGGCCGGCGCCCACAAATCACCGTGGGATGATGAGTGATCCTGCAGTAGGcg                                                                                                           >  2:256854/1‑148 (MQ=255)
                                            aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGCCCCCAAATATCCTTCAGATGAGGAGTGATCCTGGATTAgggga                                                                                                          >  2:256872/1‑146 (MQ=255)
                                            aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCGGCATTAGGCTa                                                                                                          >  2:256914/1‑149 (MQ=255)
                                              gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                          <  1:256813/147‑1 (MQ=255)
                                              gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCACCTCCCCCAAAAAAACTTCATAATATGATAGATACTGCATAATGCTATg                                                                                                        >  2:256863/1‑149 (MQ=255)
                                                   tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTAAGATGATTAGTGATCCTGCATTAGGGTATGGCGAg                                                                                                   >  1:256904/1‑149 (MQ=255)
                                                    cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                                  <  2:256814/149‑1 (MQ=255)
                                                     aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                                 >  2:256948/1‑149 (MQ=255)
                                                      gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                                <  1:256816/149‑1 (MQ=255)
                                                       ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGa                                                                                               <  2:256817/149‑1 (MQ=255)
                                                           ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                           <  2:256819/149‑1 (MQ=255)
                                                             gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                         <  2:256822/149‑1 (MQ=255)
                                                                   cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                                    <  1:256823/148‑1 (MQ=255)
                                                                    aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatc                                                                                  <  1:256824/149‑1 (MQ=255)
                                                                    aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGCTCCGCCCCCACATATACATCAGATGGTGAGTGAGCCTGCATTAGGCTATGGCGAGGGGATAAGATTTtcatc                                                                                  >  2:256921/1‑149 (MQ=255)
                                                                      tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGATATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                                <  1:256825/149‑1 (MQ=255)
                                                                      tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCGATTCCGAAAGTTAGCCGCCCCCGGCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATcac                                                                                <  2:256827/149‑1 (MQ=255)
                                                                       aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGGCGCATCGCCCAGCTAACAATCAGAGGATGAGTGATACTGCATTAGGGTATGGCAAGGTGATCGGAttttagtc                                                                                  >  2:256923/1‑142 (MQ=255)
                                                                          ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                            <  1:256829/149‑1 (MQ=255)
                                                                           cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAgggg                                                                           <  2:256831/149‑1 (MQ=255)
                                                                           cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                           <  1:256830/149‑1 (MQ=255)
                                                                             tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                         <  1:256834/149‑1 (MQ=255)
                                                                              gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGGCCCCAAATATAATTCAGATGATGAGTGATCCTGCATTAGTCGATGGCAAGGGGATCAGATTTTCATCAAAGGGGAAt                                                                        >  2:256892/1‑149 (MQ=255)
                                                                               cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                       <  1:256835/149‑1 (MQ=255)
                                                                                    tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                                  <  1:256837/149‑1 (MQ=255)
                                                                                       aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTAGCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                               <  2:256839/149‑1 (MQ=255)
                                                                                         gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                             <  2:256840/149‑1 (MQ=255)
                                                                                           cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATACTGCATTAGGCTAGGGCAAGGTGGTCAGATTTTGATCACAGGGGAATTATGATGTTAAAt                                                           >  2:256950/1‑149 (MQ=255)
                                                                                              ttCATGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                         <  1:256841/148‑1 (MQ=255)
                                                                                               tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                       <  2:256842/149‑1 (MQ=255)
                                                                                                 aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                                     <  2:256843/149‑1 (MQ=255)
                                                                                                 aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                                     <  1:256844/149‑1 (MQ=255)
                                                                                                     ggaAATCCTGATACGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCACCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAca                                                 <  2:256845/149‑2 (MQ=255)
                                                                                                      gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                                <  1:256846/149‑1 (MQ=255)
                                                                                                         aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                             <  2:256847/149‑1 (MQ=255)
                                                                                                          tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTGCAGAGGATGAGTGGTCCTGCCGTAGGCTATGGGAAGGTGATCAGCTTTTATTGACAGGGGAATTATGATGTTAATTGCATGGCACCTGCCg                                            >  2:256971/1‑149 (MQ=255)
                                                                                                           ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                           >  2:256952/1‑149 (MQ=255)
                                                                                                            cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                          <  2:256849/149‑1 (MQ=255)
                                                                                                            cTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                          <  1:256850/149‑1 (MQ=255)
                                                                                                                 ccGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGGGTGGTCCGGCATTAGGCTATGGCAAGGGGATCAGAGTTTAAGAACAGGGGGATTATGATTTTAAATGCATGGCACCTGCCGGGGcccc                                     >  2:257008/1‑149 (MQ=255)
                                                                                                                  cGGCTATTCCGAAAGTTATCCGCGCCCGTCCTCCGCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTAATCAAAGGGGATTTATGATTTTAAATTCAATGCACCTGCCGGTGccccc                                    >  2:256922/1‑149 (MQ=255)
                                                                                                                    gCTATTCCGAAAGTTATCCGGCCCCGTCCTCCTCCCCCAAATTTCCTTCAGATGATGAGTGTGCCGGCATTAGGCTATGGCAAGGGGATCAGATTTTGATCTCAGGGGGATTTTGATGTTTAATGCATGGCACCTGCCGGTGgccccct                                  >  2:256978/1‑147 (MQ=255)
                                                                                                                    gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                                  <  2:256852/149‑1 (MQ=255)
                                                                                                                    gCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                                  <  2:256853/149‑1 (MQ=255)
                                                                                                                      tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGCTGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                                <  1:256854/149‑1 (MQ=255)
                                                                                                                        ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTTATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                              <  1:256856/149‑1 (MQ=255)
                                                                                                                         tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                             <  2:256857/149‑1 (MQ=255)
                                                                                                                          ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                            <  2:256859/149‑1 (MQ=255)
                                                                                                                          ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                            <  2:256858/149‑1 (MQ=255)
                                                                                                                           cGAAAGTTATCCGCGCCCGGCCTCCGCCCCCAAATATACTAAAGATGATGAGTGATGATGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATTAATGGCACCGGCCGCTGCCCCCCATTGttta                           >  2:256928/1‑147 (MQ=255)
                                                                                                                            gAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCATTCAGATGATGAGTGTTCCTGCATTAGGGTATGGCAAGGTGATCAGATTTTGATCACAGGGGATTTATTATGTTTTTTGCATGGCGCCGGCCGGGGCCCCCATATGtttat                          >  2:256918/1‑146 (MQ=255)
                                                                                                                              aaGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTAAAGATGATGAGGGATGCTGCATTTGGGGATGGAAAGGTGATCAGATTTTGATGACAGGGGATTTATGATGTTAAATGAATGGAACCGGCCGGTGCCCCCATTTGTTaaaaa                        >  2:256912/1‑147 (MQ=255)
                                                                                                                               aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                       <  2:256862/149‑1 (MQ=255)
                                                                                                                                 ttATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATGAGATTTTAATAACAGGGGAATTATTATGTTAAATTAATGGCACCAGCCGGTGCCCCCCGTTGTTAAAcaaag                     >  2:256964/1‑149 (MQ=255)
                                                                                                                                  tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc                    <  1:256865/149‑1 (MQ=255)
                                                                                                                                  tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGATAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag                     <  1:256864/148‑1 (MQ=255)
                                                                                                                                     ccgccgccgTCCTCCTCCCCCTAATATCCTTCAGATGATGAGTGAGCCTGCAGTAGGCTATGGCAAGGTGATCAGATTTTCCTCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATCTGTTAAAcattgcaaa                 <  2:256867/149‑1 (MQ=255)
                                                                                                                                     ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa                 <  2:256868/149‑1 (MQ=255)
                                                                                                                                      cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag                <  2:256869/149‑1 (MQ=255)
                                                                                                                                      cGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTTTGGGAAGGTGATCAGATTTTCATCAAAGGGGAATTATGATGTTAAATGCATGGCACCGGCCGGTGCCCCCATTTGTTAAAcaaagcaaag                >  2:257011/1‑149 (MQ=255)
                                                                                                                                       gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa               <  2:256871/149‑1 (MQ=255)
                                                                                                                                       gCCCCCGAACTCCACCCCCAAATATACTACAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATAAGATTTTAAAAACAGGGGAATTATGATGTTTAATGCATTGCACCAGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  >  2:256935/1‑146 (MQ=255)
                                                                                                                                        cccccGTCCTCCTCCCCCAAATATCCATCAGATGATGAGTGATCCTGCATTAGGCTGTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa                  >  1:256997/1‑145 (MQ=255)
                                                                                                                                         ccccGTCCTCCTGCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAt              <  1:256873/148‑1 (MQ=255)
                                                                                                                                         ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa               <  1:256872/147‑1 (MQ=255)
                                                                                                                                          cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc             <  1:256874/148‑1 (MQ=255)
                                                                                                                                          cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa            <  1:1601292/149‑1 (MQ=255)
                                                                                                                                          cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa            >  1:256933/1‑149 (MQ=255)
                                                                                                                                              tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg        >  2:256987/1‑149 (MQ=255)
                                                                                                                                               cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc       <  2:256876/149‑1 (MQ=255)
                                                                                                                                               cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc       >  2:2441328/1‑149 (MQ=255)
                                                                                                                                                 tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                             >  1:256963/1‑93 (MQ=255)
                                                                                                                                                 tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg        >  1:1904564/1‑146 (MQ=255)
                                                                                                                                                 tcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc     <  1:256877/149‑1 (MQ=255)
                                                                                                                                                   ctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt   >  2:2345056/1‑149 (MQ=255)
                                                                                                                                                    tccCCCAAATATCCTTCAGATGATGCGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAtt  >  2:256985/1‑149 (MQ=255)
                                                                                                                                                    tccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                                    <  1:256878/83‑1 (MQ=255)
                                                                                                                                                    tccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatg                                                                    >  2:256878/1‑83 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATT  >  U00096/422287‑422583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑