Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 2,564,930 G→T A136A (GCG→GCT)  yffS → uncharacterized protein YffS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000962,564,9300GT98.0% 561.8 / ‑5.3 153A136A (GCG→GCT) yffSuncharacterized protein YffS
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (75/75);  total (77/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

AATGGATTCGAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCGGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGCAATG  >  U00096/2564785‑2565074
                                                                                                                                                 |                                                                                                                                                
 aTGGATTCGAGTTCACGCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACATGTCCCAGCAGAAGCTGAag                                                                                                                                              <  2:1598841/149‑1 (MQ=255)
  tGGATTCGAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAaga                                                                                                                                             <  2:1598842/149‑1 (MQ=255)
      ttCGAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATg                                                                                                                                         >  2:1598906/1‑149 (MQ=255)
      ttCGAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATg                                                                                                                                         >  1:1598886/1‑149 (MQ=255)
       tCGAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATg                                                                                                                                         >  1:1598938/1‑148 (MQ=255)
         gAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTTTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGa                                                                                                                                      <  2:1598844/149‑1 (MQ=255)
          aGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAg                                                                                                                                     >  2:1598889/1‑149 (MQ=255)
             tCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTg                                                                                                                                  >  2:1598845/1‑149 (MQ=255)
                 ccGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGaaaa                                                                                                                              <  2:1598847/149‑1 (MQ=255)
                 ccGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGaaaa                                                                                                                              >  1:1598948/1‑149 (MQ=255)
                    gAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGt                                                                                                                           >  1:1598896/1‑149 (MQ=255)
                     aaGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGaaaa                                                                                                                              <  1:1598848/145‑1 (MQ=255)
                        aCCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGgaa                                                                                                                       <  1:1598850/149‑1 (MQ=255)
                        aCCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGgaa                                                                                                                       >  1:1598937/1‑149 (MQ=255)
                         ccGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGgaag                                                                                                                      >  2:1598892/1‑149 (MQ=255)
                         ccGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGgaag                                                                                                                      <  2:1598851/149‑1 (MQ=255)
                           gTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCGTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGgaagaa                                                                                                                    <  2:1598852/149‑1 (MQ=255)
                             ggAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGaagaag                                                                                                                   >  1:1598899/1‑148 (MQ=255)
                                 aaaTGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGgca                                                                                                              >  1:1598984/1‑149 (MQ=255)
                                  aaTGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGgcag                                                                                                             >  2:1598919/1‑149 (MQ=255)
                                      gAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGgcag                                                                                                             >  1:1598952/1‑145 (MQ=255)
                                       aaTTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCaga                                                                                                         <  1:1598856/148‑1 (MQ=255)
                                        aTTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGGAGCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGACGCTGAAGAGATGGGAGGTGAAAAAGGGGAAGAAGTGGCAGCagag                                                                                                        <  1:1598857/148‑1 (MQ=255)
                                          tACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCaa                                                                                                     <  2:1598859/149‑1 (MQ=255)
                                           aCGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAg                                                                                                    <  2:1598860/149‑1 (MQ=255)
                                            cgcgAGGCTGTAGCTGAACGAGCTGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAgc                                                                                                   >  2:1598977/1‑149 (MQ=255)
                                             gcgAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAgcg                                                                                                  >  1:1598961/1‑149 (MQ=255)
                                                aGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGccc                                                                                               >  2:2393857/1‑149 (MQ=255)
                                                 ggCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCATCAGAGCAAGCGcc                                                                                                >  1:1598933/1‑147 (MQ=255)
                                                   cTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAAc                                                                                            <  2:1598863/149‑1 (MQ=255)
                                                   cTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAAc                                                                                            <  2:1598864/149‑1 (MQ=255)
                                                    tGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAgcg                                                                                                  >  1:1598965/1‑142 (MQ=255)
                                                      tAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGAt                                                                                         <  1:1598867/149‑1 (MQ=255)
                                                      tAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGAt                                                                                         <  2:1598866/149‑1 (MQ=255)
                                                       aGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATg                                                                                        <  1:1598868/149‑1 (MQ=255)
                                                        gCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATg                                                                                        >  1:1598930/1‑148 (MQ=255)
                                                        gCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGa                                                                                       >  2:1598993/1‑149 (MQ=255)
                                                        gCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGa                                                                                       >  1:1598951/1‑149 (MQ=255)
                                                           gAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGaaaa                                                                                    >  2:1598922/1‑149 (MQ=255)
                                                            aaCGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGaaaaa                                                                                   <  1:1598870/149‑1 (MQ=255)
                                                            aaCGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGaaaaa                                                                                   <  1:1598871/149‑1 (MQ=255)
                                                              cgcgaTGCTATTATCGACGACCTGAACGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAaga                                                                                 <  2:1598873/146‑1 (MQ=255)
                                                               gagaTGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAagag                                                                                <  2:1598875/149‑1 (MQ=255)
                                                               gagaTGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAagag                                                                                >  1:1598970/1‑149 (MQ=255)
                                                                agaTGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAag                                                                                  >  2:1598914/1‑146 (MQ=255)
                                                                 gaTGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGAt                                                                              <  2:1598876/149‑1 (MQ=255)
                                                                  aTGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATg                                                                             >  2:1598953/1‑149 (MQ=255)
                                                                     cTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCATCGATGAAAAAGAGATGGGa                                                                          <  2:1598880/149‑1 (MQ=255)
                                                                     cTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGa                                                                          <  1:1598879/149‑1 (MQ=255)
                                                                     cTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGa                                                                          >  1:1598923/1‑149 (MQ=255)
                                                                     cTATTATCGACGACCTGAAGGCCCGTAGCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGa                                                                          <  2:1598878/149‑1 (MQ=255)
                                                                       attatCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGc                                                                        <  2:1598882/149‑1 (MQ=255)
                                                                         tatCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTa                                                                      >  2:1598924/1‑149 (MQ=255)
                                                                         tatCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTa                                                                      <  2:1598883/149‑1 (MQ=255)
                                                                          atCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTaa                                                                     <  2:1598885/149‑1 (MQ=255)
                                                                            cgacgaCCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAgg                                                                   <  2:1598886/149‑1 (MQ=255)
                                                                             gacgacCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGa                                                                  >  2:1598927/1‑149 (MQ=255)
                                                                                   cTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGt                                                            <  1:1598889/149‑1 (MQ=255)
                                                                                   cTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGt                                                            >  2:1599005/1‑149 (MQ=255)
                                                                                    tGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGtt                                                           >  1:1598967/1‑149 (MQ=255)
                                                                                          cccGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTaacccg                                                     >  1:1598891/1‑149 (MQ=255)
                                                                                          cccGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGAGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTaacccg                                                     >  1:1598957/1‑149 (MQ=255)
                                                                                             gTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTaacccgaac                                                  >  2:1598893/1‑149 (MQ=255)
                                                                                             gTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTaacc                                                       <  1:1598894/144‑1 (MQ=255)
                                                                                                  gCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAAcccgaacccgaa                                             >  2:1598976/1‑149 (MQ=255)
                                                                                                  gCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAAcccgaacccgaa                                             <  2:1598896/149‑1 (MQ=255)
                                                                                                   cTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGaaa                                            <  2:1598898/149‑1 (MQ=255)
                                                                                                    tGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGaaaa                                           >  1:1598985/1‑149 (MQ=255)
                                                                                                    tGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCGGAAGAGATGGGACGTGAAAAAGTGGAAGACGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGCAGCTAAGGAGTGGGTTAACACGAACACGataa                                           >  2:1598991/1‑149 (MQ=255)
                                                                                                     gAGCTTGCGGCGGCACTAGCAAATAAACACGTCCCAGCAGAAGCGGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAAc                                                                                            <  1:1598900/99‑1 (MQ=255)
                                                                                                     gAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGaaaaa                                          <  2:1598902/149‑1 (MQ=255)
                                                                                                     gAGCTTGAGGCGGCACTAGCAAATAAACAAGGCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGaaaaa                                          <  2:1598901/149‑1 (MQ=255)
                                                                                                      aGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGaaaaaa                                         >  1:1598943/1‑149 (MQ=255)
                                                                                                         ttGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAAtcc                                      >  1:1599014/1‑147 (MQ=255)
                                                                                                            aGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCATGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGc                                   <  2:1598905/149‑1 (MQ=255)
                                                                                                                 gcACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGCGCCAGCGCCCAACGATGAAAAAGAGATGGGCGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACCGCGTCgg                              >  1:1599000/1‑149 (MQ=255)
                                                                                                                 gcACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTc                                <  1:1598909/147‑1 (MQ=255)
                                                                                                                 gcACTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCgg                              >  2:1599010/1‑149 (MQ=255)
                                                                                                                   aCTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCa                            <  2:1598910/149‑1 (MQ=255)
                                                                                                                   aCTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCa                            <  2:1598911/149‑1 (MQ=255)
                                                                                                                    cTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCAt                           >  2:1598962/1‑149 (MQ=255)
                                                                                                                    cTAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCAt                           >  2:1599030/1‑149 (MQ=255)
                                                                                                                     tAGCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCAtg                          >  2:1598980/1‑149 (MQ=255)
                                                                                                                       gCAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTACCCCGAACCCGAAAAAATACAGCGTCGGCAtgtt                        >  2:1598913/1‑149 (MQ=255)
                                                                                                                        cAAATAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTa                                                          >  1:1599026/1‑114 (MQ=255)
                                                                                                                         aaaTAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCAtgttgt                      >  1:1598969/1‑149 (MQ=255)
                                                                                                                         aaaTAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCAtgttgt                      >  2:1598932/1‑149 (MQ=255)
                                                                                                                            tAAACAAGTCCCAGCAGCAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCACGCGCCCAACGATGAAAAAGAGATGGGTGCTAAGGAGTGGGTTAAACCGAACCCGAAAAATTACAGCGTCGGCATGTTGTGTc                   >  2:1598982/1‑149 (MQ=255)
                                                                                                                            tAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACCGCGTCGGCATGTTgtgtg                   >  2:1599036/1‑148 (MQ=255)
                                                                                                                            tAAACAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTc                   >  1:1598941/1‑149 (MQ=255)
                                                                                                                               aCAAGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAgg                >  1:1598947/1‑149 (MQ=255)
                                                                                                                                cAAGTCCCAGCAGAAGCTGAAGAGATGGGATGTGAAAAAGTGGAAGAAGTGCCAGCAGAGCAAGAGCCCAACGATGAAAAAGAGATGGGAGCAAAGGAGTGGGTTAACCCGATCCCGAAAAAATACAGCGTCTCCATGTTGTGTCAGGt               >  1:1599033/1‑149 (MQ=255)
                                                                                                                                  aGTCCCAGCAGAAGCTGAAGAGATGGGAGGTGaaaaa                                                                                                                             <  1:1598915/37‑1 (MQ=255)
                                                                                                                                     cccAGCAGAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTgg          <  1:1598917/149‑1 (MQ=255)
                                                                                                                                        agcagAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTg       >  1:1599003/1‑149 (MQ=255)
                                                                                                                                         gcagAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGc      >  1:1598959/1‑149 (MQ=255)
                                                                                                                                         gcagAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGc      <  2:1598918/149‑1 (MQ=255)
                                                                                                                                          cagAAGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGCa     >  1:1599047/1‑149 (MQ=255)
                                                                                                                                             aaGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGCAATg  >  2:1598966/1‑149 (MQ=255)
                                                                                                                                             aaGCTGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGCAATg  <  1:1598921/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AATGGATTCGAGTTCACCCGGAAGACCGTGGAGAAATGGAATTACGCGAGGCTGTAGCTGAACGAGATGCTATTATCGACGACCTGAAGGCCCGTATCGCTGAGCTTGAGGCGGCACTAGCAAATAAACAAGTCCCAGCAGAAGCGGAAGAGATGGGAGGTGAAAAAGTGGAAGAAGTGGCAGCAGAGCAAGCGCCCAACGATGAAAAAGAGATGGGAGCTAAGGAGTGGGTTAACCCGAACCCGAAAAAATACAGCGTCGGCATGTTGTGTCAGGTACTGGCTGCAATG  >  U00096/2564785‑2565074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑