BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	754,123	C→T	E116K (GAG→AAG)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	754,123	0	C	T	99.0%	380.3 / ‑4.3	104	E116K (GAG→AAG)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (51/52); total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 107 total aligned reads displayed.

CGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGC  >  U00096/753978‑754268

cGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATCCCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTtgtg                                                                                                                                                >  2:296714/1‑149 (MQ=255)

cGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTtgtg                                                                                                                                                <  2:296630/149‑1 (MQ=255)

cGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTtgtg                                                                                                                                                <  2:296631/149‑1 (MQ=255)

   aaCGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGAt                                                                                                                                             >  2:296679/1‑149 (MQ=255)

     cgcgGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCa                                                                                                                                           >  2:296695/1‑149 (MQ=255)

      gcgGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCCGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCAt                                                                                                                                          >  2:296632/1‑149 (MQ=255)

       cgGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAAGCTGCTTGTGGATCATg                                                                                                                                         <  2:296633/149‑1 (MQ=255)

        ggCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATgg                                                                                                                                        >  1:296681/1‑149 (MQ=255)

         gCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGt                                                                                                                                       >  1:296700/1‑149 (MQ=255)

          cAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTa                                                                                                                                      <  1:296634/149‑1 (MQ=255)

          cAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTa                                                                                                                                      <  2:296635/149‑1 (MQ=255)

           aaTTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTAt                                                                                                                                     >  1:296684/1‑149 (MQ=255)

            aTTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATg                                                                                                                                    >  2:296726/1‑149 (MQ=255)

             tttCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAGCAGACGGGTAATCTGCTTGTGGATCATGGTATGa                                                                                                                                   <  2:296636/149‑1 (MQ=255)

             tttCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGa                                                                                                                                   <  2:296637/149‑1 (MQ=255)

             tttCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGa                                                                                                                                   >  1:296672/1‑149 (MQ=255)

               tCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACg                                                                                                                                 <  2:296638/149‑1 (MQ=255)

                  cGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGt                                                                                                                              >  1:296664/1‑149 (MQ=255)

                  cGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCAATGGATGCGAGGCGCGACGGAAAGCAGGGAACAGACGGGTAATCTGCATGTGGATCATGGTATGACGGGt                                                                                                                              <  2:296639/149‑1 (MQ=255)

                      gACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCAcc                                                                                                                          <  2:296641/149‑1 (MQ=255)

                        cGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGt                                                                                                                        >  2:296693/1‑149 (MQ=255)

                         gAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTa                                                                                                                       <  1:296642/149‑1 (MQ=255)

                          aGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAg                                                                                                                      >  2:296683/1‑149 (MQ=255)

                              ttGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGtttt                                                                                                                  >  2:296660/1‑149 (MQ=255)

                                 ttAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTaaa                                                                                                               <  2:296643/149‑1 (MQ=255)

                                   aaCATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAAtt                                                                                                             <  2:296644/149‑1 (MQ=255)

                                      aTCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGt                                                                                                          >  1:296710/1‑149 (MQ=255)

                                      aTCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGt                                                                                                          <  1:1582554/149‑1 (MQ=255)

                                       tCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTc                                                                                                         >  2:296685/1‑149 (MQ=255)

                                        ccAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCa                                                                                                        <  2:296646/149‑1 (MQ=255)

                                            cGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACt                                                                                                    <  2:296648/149‑1 (MQ=255)

                                             gAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACt                                                                                                    >  1:296675/1‑148 (MQ=255)

                                                tCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTc                                                                                                >  1:296736/1‑149 (MQ=255)

                                                 cGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTcc                                                                                               <  2:296649/149‑1 (MQ=255)

                                                  gTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCt                                                                                              <  2:296651/149‑1 (MQ=255)

                                                  gTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCt                                                                                              <  1:296650/149‑1 (MQ=255)

                                                   tGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTg                                                                                             >  2:296704/1‑149 (MQ=255)

                                                    gATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGa                                                                                            <  1:296652/149‑1 (MQ=255)

                                                       aGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTc                                                                                         >  1:296744/1‑149 (MQ=255)

                                                       aGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTc                                                                                         <  1:296653/149‑1 (MQ=255)

                                                           cgccgcCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGtt                                                                                     >  2:1255512/1‑149 (MQ=255)

                                                            gccgccAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTc                                                                                         <  1:296654/144‑1 (MQ=255)

                                                             ccgccAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGtttt                                                                                   >  1:296706/1‑149 (MQ=255)

                                                                ccAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTc                                                                                 >  1:296717/1‑148 (MQ=255)

                                                                     gcgcCGTTAATACCAGACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGTACAGACGGGTAATCTGCTTGTGTATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAt                                                                            <  1:296655/148‑1 (MQ=255)

                                                                      cgcCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTc                                                                          <  2:296656/149‑1 (MQ=255)

                                                                      cgcCGGTAATACCACACATGACTGCCAATGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTc                                                                          <  2:1537813/149‑1 (MQ=255)

                                                                       gcCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTca                                                                         >  2:296747/1‑149 (MQ=255)

                                                                          ggTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagca                                                                      <  2:296658/149‑1 (MQ=255)

                                                                          ggTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagca                                                                      >  2:296719/1‑149 (MQ=255)

                                                                          ggTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGAGCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagca                                                                      <  2:296657/149‑1 (MQ=255)

                                                                           gTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACAATTcagcag                                                                     >  2:296728/1‑149 (MQ=255)

                                                                            tAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAgg                                                                    >  1:296707/1‑149 (MQ=255)

                                                                            tAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGAAATCTGCTTGTGGATCATGGTATGACGGGTCACCGAAGTTTAAAATACGTCAAACTGATCCTGAGTCGCTTTTTCACCATTCAGCAgg                                                                    >  2:296697/1‑149 (MQ=255)

                                                                               tACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGAAGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTATTCATACTGTTACTGAGTCGGTTTTTCACCATTCAGCAGGATg                                                                 >  2:296663/1‑149 (MQ=255)

                                                                                 ccACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTa                                                               <  1:296659/149‑1 (MQ=255)

                                                                                   acacaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAAc                                                             <  2:296661/149‑1 (MQ=255)

                                                                                   acacaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAAc                                                             <  1:296660/149‑1 (MQ=255)

                                                                                    cacaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACa                                                            <  1:296662/149‑1 (MQ=255)

                                                                                     acctGACTGCCTTTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACaa                                                           <  1:296663/146‑1 (MQ=255)

                                                                                         gACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACtt                                                       >  2:296731/1‑149 (MQ=255)

                                                                                          aCTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTc                                                      <  2:296664/149‑1 (MQ=255)

                                                                                           cTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTcc                                                     <  1:296665/149‑1 (MQ=255)

                                                                                           cTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTcc                                                     >  2:296694/1‑149 (MQ=255)

                                                                                            tGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCa                                                    >  1:1242758/1‑149 (MQ=255)

                                                                                               cATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGt                                                 <  1:296667/149‑1 (MQ=255)

                                                                                                aTTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTa                                                >  1:296737/1‑149 (MQ=255)

                                                                                                 ttGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAg                                               <  1:296668/149‑1 (MQ=255)

                                                                                                   ggATGCGAGTCGCGACGGAACGCATGGAACAGACGGGTAATCTGCTTGTGGAGCATGGTATGACGGGGCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGtt                                             <  2:296669/149‑1 (MQ=255)

                                                                                                    ttggCGAGTCGCGACGGAAAGCTTGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTa                                            <  1:296671/146‑1 (MQ=255)

                                                                                                    gATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTa                                            >  2:296746/1‑149 (MQ=255)

                                                                                                     aTGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAg                                           <  2:296670/149‑1 (MQ=255)

                                                                                                      tGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGtt                                             >  2:296739/1‑146 (MQ=255)

                                                                                                        cGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAAt                                        >  1:296718/1‑149 (MQ=255)

                                                                                                           gTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGGAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCgg                                     >  2:296755/1‑149 (MQ=255)

                                                                                                             cgcgACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTc                                   <  2:296672/149‑1 (MQ=255)

                                                                                                              gcgACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCg                                  >  1:296724/1‑149 (MQ=255)

                                                                                                                gACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGc                                 >  1:296716/1‑148 (MQ=255)

                                                                                                                  cGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg                              >  2:296738/1‑149 (MQ=255)

                                                                                                                  cGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg                              >  2:296740/1‑149 (MQ=255)

                                                                                                                       aGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgat                         <  1:296673/149‑1 (MQ=255)

                                                                                                                       aGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgat                         <  1:296674/149‑1 (MQ=255)

                                                                                                                        gCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatc                        <  2:296675/149‑1 (MQ=255)

                                                                                                                         cATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcg                       <  2:296676/149‑1 (MQ=255)

                                                                                                                         cATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcg                       >  2:296723/1‑149 (MQ=255)

                                                                                                                          aTGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga                      <  1:296677/149‑1 (MQ=255)

                                                                                                                            ggAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcgatc                    <  1:296678/149‑1 (MQ=255)

                                                                                                                            ggAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcgatc                    <  1:296679/149‑1 (MQ=255)

                                                                                                                             gAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGatcgatcg                   >  2:296762/1‑149 (MQ=255)

                                                                                                                             gAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGatcgatcg                   >  1:296743/1‑149 (MQ=255)

                                                                                                                              aaCAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCgg                  >  1:296764/1‑149 (MQ=255)

                                                                                                                                 aGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGaa               <  2:296681/149‑1 (MQ=255)

                                                                                                                                 aGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGaa               <  2:296680/149‑1 (MQ=255)

                                                                                                                                       gTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACcgcg         >  1:296732/1‑149 (MQ=255)

                                                                                                                                        tAATCTGCTTTTGGATCATGGTATGACGGGTCACCTTAGTTTTAAATTCGTCATACTGTTCCTTATGCGGGTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCTATCGGGAAACCGTgg        <  2:296682/149‑1 (MQ=255)

                                                                                                                                           tCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgc     >  2:296727/1‑149 (MQ=255)

                                                                                                                                             tGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgcag   >  1:735084/1‑149 (MQ=255)

                                                                                                                                              gCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGgcgc     <  1:296685/146‑1 (MQ=255)

                                                                                                                                              gCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgcagc  <  2:296684/149‑1 (MQ=255)

                                                                                                                                              gCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgc     <  1:296683/146‑1 (MQ=255)

CGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGC  >  U00096/753978‑754268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`