Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	1,618,245	A→T	*222K (TAA→AAA)	marC ←	inner membrane protein MarC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	1,618,245	0	A	T	100.0%	310.2 / NA	81	*222K (TAA→AAA)	marC	inner membrane protein MarC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (42/39);  total (42/39)

TGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGG  >  U00096/1618100‑1618387                                                                                                                                                  |                                                                                                                                               tGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCACGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATg                                                                                                                                             <  2:622443/149‑1 (MQ=255)  gCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATgg                                                                                                                                            >  1:622466/1‑149 (MQ=255)    aCTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTa                                                                                                                                          >  1:622510/1‑149 (MQ=255)      tGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACg                                                                                                                                        >  2:622490/1‑149 (MQ=255)        gTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGtt                                                                                                                                      <  1:622445/149‑1 (MQ=255)         tCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCATTAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGttt                                                                                                                                     >  2:622494/1‑149 (MQ=255)             tGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAAt                                                                                                                                 <  2:622446/149‑1 (MQ=255)                 gatgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGAtt                                                                                                                             <  1:622447/149‑1 (MQ=255)                   tgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTc                                                                                                                           <  2:622448/149‑1 (MQ=255)                    gatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTc                                                                                                                           >  1:622506/1‑148 (MQ=255)                       tGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAgg                                                                                                                       <  1:622449/149‑1 (MQ=255)                         gTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCa                                                                                                                         <  1:622451/145‑1 (MQ=255)                         gTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGAt                                                                                                                     <  2:622450/149‑1 (MQ=255)                            aTGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  <  2:622454/149‑1 (MQ=255)                            aTGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  <  2:622453/149‑1 (MQ=255)                            aTGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  >  2:622503/1‑149 (MQ=255)                            aTGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  <  2:622452/149‑1 (MQ=255)                            aTGTGGGCGCGGTGCATGCTTTTGCCGCGTTAATTTGTTCAATCATTATATTTCGCCGCTTGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  <  2:622455/149‑1 (MQ=255)                              gtgGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                                                                >  1:622499/1‑149 (MQ=255)                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaata                                                                                                           <  2:622456/149‑1 (MQ=255)                                   cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGGCAATCATTATAATTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaata                                                                                                           <  2:622457/149‑1 (MQ=255)                                     cgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataa                                                                                                          <  1:622458/148‑1 (MQ=255)                                      ggTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                                                                        >  1:622475/1‑149 (MQ=255)                                        tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATaaa                                                                                                      <  2:622460/149‑1 (MQ=255)                                        tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATaaa                                                                                                      <  1:622459/149‑1 (MQ=255)                                         gCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAAc                                                                                                     <  2:622461/149‑1 (MQ=255)                                          ccTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAAc                                                                                                     >  1:622488/1‑148 (MQ=255)                                            tGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                                                                                  >  1:622480/1‑149 (MQ=255)                                                tttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                                                                                               <  1:622462/148‑1 (MQ=255)                                                tttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                                                                                              >  2:622501/1‑149 (MQ=255)                                                  tGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACAccc                                                                                            >  2:622519/1‑149 (MQ=255)                                                    ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcat                                                                                          >  2:622485/1‑149 (MQ=255)                                                    ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcat                                                                                          <  2:622463/149‑1 (MQ=255)                                                        gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcatacat                                                                                      >  2:622531/1‑149 (MQ=255)                                                        gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcatacat                                                                                      >  1:622495/1‑149 (MQ=255)                                                             tttGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAAAAATAAACTGcac                                                                                                <  1:622464/134‑1 (MQ=255)                                                               tGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACcagca                                                                               >  2:622479/1‑149 (MQ=255)                                                               tGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACcagca                                                                               >  2:622521/1‑149 (MQ=255)                                                                ggTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACcagcag                                                                              <  2:622465/149‑1 (MQ=255)                                                                ggTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACcagcag                                                                              >  2:622484/1‑149 (MQ=255)                                                                ggTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACcagcag                                                                              >  1:622496/1‑149 (MQ=255)                                                                  tCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGa                                                                            <  2:622466/149‑1 (MQ=255)                                                                    aaTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAAt                                                                          >  1:622482/1‑149 (MQ=255)                                                                     aTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACca                                                                                  <  1:622467/140‑1 (MQ=255)                                                                     aTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATc                                                                         >  1:622518/1‑149 (MQ=255)                                                                       cATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGCTGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATccc                                                                       >  2:622509/1‑149 (MQ=255)                                                                       cATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATccc                                                                       >  1:622492/1‑149 (MQ=255)                                                                       cATTATATATCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATccc                                                                       <  2:622468/149‑1 (MQ=255)                                                                        aTTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATcc                                                                        >  1:622517/1‑147 (MQ=255)                                                                        aTTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATc                                                                         >  1:622493/1‑146 (MQ=255)                                                                         ttATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt                                                                     >  2:622491/1‑149 (MQ=255)                                                                           atatTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcac                                                                                                >  1:622498/1‑120 (MQ=255)                                                                           atatTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATc                                                                    <  1:622469/148‑1 (MQ=255)                                                                             atTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGa                                                                 <  1:622470/149‑1 (MQ=255)                                                                              tttCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGAc                                                                >  1:622516/1‑149 (MQ=255)                                                                               ttCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACg                                                               >  2:622487/1‑149 (MQ=255)                                                                               ttCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATACTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCAAGGATGCCGTTAATAATAAACTGCACACCCATACATACAAGCAGGAATCCCATCAGACg                                                               >  2:622505/1‑149 (MQ=255)                                                                                 cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACggg                                                             <  1:622471/149‑1 (MQ=255)                                                                                    cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACggg                                                             >  1:622500/1‑146 (MQ=255)                                                                                     gcTGGCCAGTGACCCTCGAACAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGAACGTTTTAATGATTTCCAGGATGCCGTAAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGAt                                                         <  2:622473/149‑1 (MQ=255)                                                                                     gcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACggg                                                             >  2:622489/1‑145 (MQ=255)                                                                                     gcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATATCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGAt                                                         <  2:622472/149‑1 (MQ=255)                                                                                        ggCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGc                                                      >  1:622535/1‑149 (MQ=255)                                                                                           cAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTc                                                   <  2:622474/149‑1 (MQ=255)                                                                                              tGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAAt                                                <  2:622475/149‑1 (MQ=255)                                                                                                                   aaTATTTGCAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTATTAATAAACTGCACACCCATACATACCATCAGTAATCCCATCAGACGGGAGTTCGCTTCAATGCCACCCTTGCCCACCAGcgg                           <  2:622476/149‑3 (MQ=255)                                                                                                                   aaTAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCg                           >  2:622478/1‑149 (MQ=255)                                                                                                                   aaTAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCg                           >  2:622526/1‑149 (MQ=255)                                                                                                                    aTAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGc                          >  2:622533/1‑149 (MQ=255)                                                                                                                     tAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGc                          >  1:622502/1‑148 (MQ=255)                                                                                                                       gTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATa                       >  2:622545/1‑149 (MQ=255)                                                                                                                          gAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAAtt                    >  2:622497/1‑149 (MQ=255)                                                                                                                           aaaGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTg                   >  1:622529/1‑149 (MQ=255)                                                                                                                              ggCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTgcgc                <  1:622478/149‑1 (MQ=255)                                                                                                                                   aTTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAg           <  1:622479/149‑1 (MQ=255)                                                                                                                                    ttCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCa                                                                                                                         <  1:622481/38‑1 (MQ=255)                                                                                                                                    ttCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGc          <  2:622480/149‑1 (MQ=255)                                                                                                                                         gCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGt     >  2:622515/1‑149 (MQ=255)                                                                                                                                          ccTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAACTGCGCCGGAGCTGCGTa    <  2:622482/149‑1 (MQ=255)                                                                                                                                            ttttttTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAGTAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAgg  >  2:622483/1‑149 (MQ=255)                                                                                                                                            ttttttTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAgg  <  1:622484/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                               TGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGG  >  U00096/1618100‑1618387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑