Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,708,7510AG21.1% 56.7 / 10.1 39E611E (GAA→GAG) rsxCSoxR [2Fe‑2S] reducing system protein RsxC
Reads supporting (aligned to +/- strand):  ref base A (10/20);  new base G (5/3);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATT  >  U00096/1708609‑1708895
                                                                                                                                              |                                                                                                                                                
acctcgcgAAAGCGCTCAAGCAGGAACATCATCAGGCTACTCAGGACGCAGCACCAGAGGCCGATCCTCGTAAATCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaa                                                                                                                                            <  2:1427741/145‑1 (MQ=9)
    ctgcAAAGCGCGCAAGCTGGAACAGCTACAGTCTAATGCGGAAACAGAACAACATGTCTATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAc                                                                                                                                        <  1:2622052/146‑1 (MQ=14)
                 aaGCTGGCAGACCATCAGGCAAATGACGACCCAGCACCCCTGGGCGATCCTCGCAAATCCGCCGTCGAAGCCGCTATTTCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgcg                                                                                                                           <  1:1427728/149‑1 (MQ=14)
                    cTGGAACAGCATCAGGATAATCCGGACGCAGAACACCAGGGCGATCCGCGAAAAGCCGCCGTCGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCaa                                                                                                                        <  1:1427715/149‑1 (MQ=14)
                         aCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg                                                                                                                   <  1:1427717/149‑1 (MQ=255)
                               aCAGGCTAATTCGGTACCACCACATCTTGCCGATCCTCGCAAATCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCg                                                                                                             <  2:1427729/149‑1 (MQ=14)
                                cAGGCTAATCAGGACCCAGCACCACATGCCGATCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGa                                                                                                            <  2:1427731/149‑1 (MQ=14)
                                    atacttaggAAGCAGAAAAAGTTGTCGATCCTCGCAAACCCGCCGTTGAAGCAGCTTTAGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGcc                                                                                                        <  2:1427725/142‑1 (MQ=11)
                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGcc                                                                                               >  2:2010044/1‑149 (MQ=255)
                                              aCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGccc                                                                                              <  2:1427723/149‑1 (MQ=255)
                                                      accaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa                                                                                      <  2:1427724/147‑1 (MQ=255)
                                                         aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCa                                                                                                                                               >  2:1427874/1‑89 (MQ=255)
                                                              gATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg                                                                                                                                      <  1:1427735/93‑1 (MQ=255)
                                                              gATCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                                     <  1:1427734/94‑1 (MQ=255)
                                                              gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACa                                                                                                                                       <  1:1427737/92‑1 (MQ=255)
                                                                    ccccaacccgccgccgAAGCCCCTATCGCCCGCGTCAAAGCTATCTAGATGGTACAGCAACCTGCTAAACAGTAAACAGAACCACCTCTCGATCCGCTCATATCTGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGa                                                                        <  2:1427772/142‑1 (MQ=2)
                                                                      ccaccccgccgTCGAAGCCGCTATCGCCCGCGCCAAATTTCTAAAGCTTTAAGTGGAACAGGGTTATGTTGAACCATAACAACATGGCGTCGCTTGCTAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAAc                                                                      <  2:1427768/144‑1 (MQ=2)
                                                                       accgccgccCTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTTTAACTGCAACTGGCTAATGCGGAACCAGAAGAACAGGTCTATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa                                                                     <  2:1427738/146‑1 (MQ=11)
                                                                       aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa                                                                     <  1:2693607/149‑1 (MQ=255)
                                                                         cccccccgccgAAGCCGCTCTCGCCCGCGCCAACGCGCTCTAGCTGTAACTGCAACGGGCTAATGCGGAACCACAACACCATGCCGATCCGCGCATCTCCGCAGCCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGc                                                                   <  2:1427744/144‑1 (MQ=9)
                                                                            cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCACGAGCCAAAGCACGCAAGCTGGAACAGCagc                                                                >  2:1427828/1‑147 (MQ=11)
                                                                                    aaGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGt                                                                                                                                    <  1:1427746/73‑1 (MQ=255)
                                                                                      gCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCaga                                                                                                                                             >  1:1427821/1‑62 (MQ=255)
                                                                                        cGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATc                                                                                                                               >  1:1427747/1‑74 (MQ=255)
                                                                                          cTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAc                                                                                                                                        >  1:1427907/1‑63 (MQ=255)
                                                                                          cTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAc                                                                                                                                        <  2:1427907/63‑1 (MQ=255)
                                                                                                             gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                                    <  1:1427842/48‑1 (MQ=255)
                                                                                                             gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                                    >  2:1427842/1‑48 (MQ=255)
                                                                                                             gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc                                                                                                                              >  1:1427933/1‑54 (MQ=11)
                                                                                                             gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc                                                                                                                              <  2:1427933/54‑1 (MQ=11)
                                                                                                               gcAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTc                                                                                                              <  2:1427849/68‑1 (MQ=11)
                                                                                                               gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTc                                                                                                              >  1:1427849/1‑68 (MQ=25)
                                                                                                               gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTc                                                                                                              >  1:1427850/1‑68 (MQ=25)
                                                                                                                 aaGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAgc                                                                                                                     >  2:1427843/1‑59 (MQ=255)
                                                                                                                  aGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAgctgc                                                                                                                  >  2:1427846/1‑61 (MQ=11)
                                                                                                                        aaCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAgc                                                                                                                     >  1:1427851/1‑52 (MQ=255)
                                                                                                                            gCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                >  1:1427801/1‑69 (MQ=35)
                                                                                                                                     tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAACCGCGCAATGCCGCCTTccgtgacg       >  2:1427752/1‑142 (MQ=14)
                                                                                                                                          cGGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAtt  <  2:1427755/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATT  >  U00096/1708609‑1708895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑