Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	3,153,487	Δ15 bp	intergenic (+64/‑62)	metC → / → yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	3153487	3153501	15	89 [0]	[0] 89	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 3153486	0 (0.000)	86 (1.020)	71/278	0.2	100%	intergenic (+63/‑77)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB
	?	U00096	3153502 =	0 (0.000)					intergenic (+79/‑61)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

TTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTA  >  U00096/3153359‑3153648                                                                                                                                                |                                                                                                                                                   tgattcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGAc                                                                                                                                                >  1:950065‑M2/144‑148 (MQ=255)     tcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGAtt                                                                                                                                            >  2:950084‑M2/140‑148 (MQ=255)             atattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTa                                                                                                                                    <  2:950030‑M2/17‑1 (MQ=255)             atattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTa                                                                                                                                    <  2:950031‑M2/17‑1 (MQ=255)                 tggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAAt                                                                                                                                <  2:950032‑M2/21‑1 (MQ=255)                  ggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATa                                                                                                                               <  2:950034‑M2/22‑1 (MQ=255)                  ggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATa                                                                                                                               <  2:950033‑M2/22‑1 (MQ=255)                  ggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATa                                                                                                                               <  2:950035‑M2/22‑1 (MQ=255)                       ggaagatgtcgacgatctgattgccgatctggacgccgtttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAgg                                                                                                                             <  2:950036‑M2/24‑1 (MQ=255)                       ggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAg                                                                                                                          >  2:950090‑M2/122‑148 (MQ=255)                        gaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGc                                                                                                                            >  1:950072‑M2/121‑145 (MQ=255)                             tgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAg                                                                                                                    >  2:950085‑M2/116‑148 (MQ=255)                             tgtcgacgacctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAg                                                                                                                    >  2:950101‑M2/116‑148 (MQ=255)                                  acgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTa                                                                                                               >  2:950061‑M2/111‑148 (MQ=255)                                   cgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                              >  1:950063‑M2/110‑148 (MQ=255)                                     atctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAAc                                                                                                            >  1:950078‑M2/108‑148 (MQ=255)                                       ctgcttgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtacCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGc                                                                                                          >  2:950070‑M2/106‑148 (MQ=255)                                       ctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGc                                                                                                          <  1:950037‑M2/43‑1 (MQ=255)                                        tgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCt                                                                                                         <  1:950038‑M2/44‑1 (MQ=255)                                        tgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCt                                                                                                         >  1:950077‑M2/105‑148 (MQ=255)                                         gattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCtt                                                                                                                <  2:950039‑M2/37‑1 (MQ=255)                                         gattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                              <  2:950041‑M2/39‑1 (MQ=255)                                         gattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACg                                                                                                           <  1:950040‑M2/42‑1 (MQ=255)                                          attgccgatctggccggcggttttgcgcgaattgtataacattgcctcttatggacaattttgcagtcattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTTAACGCTGt                                                                                                       <  2:950042‑M2/46‑1 (MQ=255)                                           ttgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGtt                                                                                                      >  1:950067‑M2/102‑148 (MQ=255)                                                 atctggacgccggttttgagcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAAAAGGCAGATAAAGGCTTAAACGCTGTTCCACAg                                                                                                >  1:950076‑M2/96‑148 (MQ=255)                                                  tctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAgg                                                                                               >  2:950075‑M2/95‑148 (MQ=255)                                                  tctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAgg                                                                                               >  1:950066‑M2/95‑148 (MQ=255)                                                     ggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCcaca                                                                                                 <  1:950044‑M2/52‑1 (MQ=255)                                                      gacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAg                                                                                           >  1:950068‑M2/91‑148 (MQ=255)                                                      gacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAg                                                                                           >  1:950073‑M2/91‑148 (MQ=255)                                                      gacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAg                                                                                           <  1:950045‑M2/58‑1 (MQ=255)                                                      gacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAg                                                                                           >  2:950043‑M2/91‑148 (MQ=255)                                                        cgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTc                                                                                         <  1:950046‑M2/60‑1 (MQ=255)                                                         gccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTcc                                                                                        <  1:950048‑M2/61‑1 (MQ=255)                                                         gccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTc                                                                                         <  1:950047‑M2/60‑1 (MQ=255)                                                            ggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTcc                                                                                        <  1:950049‑M2/61‑1 (MQ=255)                                                              ttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGc                                                                                   <  2:950050‑M2/66‑1 (MQ=255)                                                                  gcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGAAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGtt                                                                               >  1:950100‑M2/79‑148 (MQ=255)                                                                     cgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTAtt                                                                            <  2:950051‑M2/73‑1 (MQ=255)                                                                       aattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTc                                                                           <  1:950052‑M2/74‑1 (MQ=255)                                                                         ttgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAg                                                                        <  1:950053‑M2/77‑1 (MQ=255)                                                                          tgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCa                                                                          >  2:950103‑M2/71‑145 (MQ=255)                                                                          tgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa                                                                       <  2:950054‑M2/78‑1 (MQ=255)                                                                           gtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa                                                                       <  1:950055‑M2/78‑1 (MQ=255)                                                                             ataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGatat                                                                    >  2:950099‑M2/68‑148 (MQ=255)                                                                              taacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc                                                                   >  1:950074‑M2/67‑148 (MQ=255)                                                                               aacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatca                                                                  <  1:950056‑M2/83‑1 (MQ=255)                                                                                   ttgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc                                                              >  2:950057‑M2/62‑148 (MQ=255)                                                                                   ttgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc                                                              >  2:950105‑M2/62‑148 (MQ=255)                                                                                    tgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCt                                                             >  2:950096‑M2/61‑148 (MQ=255)                                                                                        acttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcg                                                          >  1:950087‑M2/57‑147 (MQ=255)                                                                                         cttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGct                                                        >  2:950111‑M2/56‑148 (MQ=255)                                                                                          ttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctc                                                       >  2:950116‑M2/55‑148 (MQ=255)                                                                                            ttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGGTATTCAAGATATCATCGCTGCGCTCTg                                                     >  1:950086‑M2/53‑148 (MQ=255)                                                                                              ggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc                                                   >  1:950112‑M2/51‑148 (MQ=255)                                                                                                 caattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTg                                                                                                        >  1:950125‑M2/48‑92 (MQ=255)                                                                                                  aattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                               >  1:950129‑M2/47‑148 (MQ=255)                                                                                                  aattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                               <  2:950060‑M2/102‑1 (MQ=255)                                                                                                  aattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                               <  1:950059‑M2/102‑1 (MQ=255)                                                                                                   attttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa                                                 >  1:950093‑M2/46‑145 (MQ=255)                                                                                                    ttttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCGATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACg                                             >  2:950109‑M2/45‑148 (MQ=255)                                                                                                    ttttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACg                                             <  1:950061‑M2/104‑1 (MQ=255)                                                                                                    ttttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACg                                             >  2:950094‑M2/45‑148 (MQ=255)                                                                                                      ttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACCCGAc                                           >  2:950114‑M2/43‑148 (MQ=255)                                                                                                       tgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACt                                          <  2:950062‑M2/107‑1 (MQ=255)                                                                                                        gcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACtt                                         >  2:950083‑M2/41‑148 (MQ=255)                                                                                                           gacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                                      >  2:950080‑M2/38‑148 (MQ=255)                                                                                                             cattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcg                                    <  2:950063‑M2/113‑1 (MQ=255)                                                                                                               ttttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAAGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgcg                                  <  2:950064‑M2/115‑1 (MQ=255)                                                                                                                tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGCTATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                 >  1:950089‑M2/33‑148 (MQ=255)                                                                                                                tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                 <  2:950065‑M2/116‑1 (MQ=255)                                                                                                                tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                 >  2:950113‑M2/33‑148 (MQ=255)                                                                                                                tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATAATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                 >  1:950095‑M2/33‑148 (MQ=255)                                                                                                                 ttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt                                >  2:950091‑M2/32‑148 (MQ=255)                                                                                                                    ttgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGTCAACACGACTTTGCCGCGCTGGc                             >  2:950079‑M2/29‑148 (MQ=255)                                                                                                                     tgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTGCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg                            >  2:950106‑M2/28‑148 (MQ=255)                                                                                                                       tgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa                          >  2:950120‑M2/26‑148 (MQ=255)                                                                                                                           aagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATcc                       >  1:950124‑M2/22‑147 (MQ=255)                                                                                                                              gcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCat                   <  2:1425491‑M2/130‑1 (MQ=255)                                                                                                                                ttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCatat                 <  2:950066‑M2/132‑1 (MQ=255)                                                                                                                                   aattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGt              >  1:950136‑M2/14‑148 (MQ=255)                                                                                                                                    attgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt             >  1:950107‑M2/13‑148 (MQ=255)                                                                                                                                     ttgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTa            >  2:950088‑M2/12‑148 (MQ=255)                                                                                                                                     ttgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTa            <  2:950068‑M2/137‑1 (MQ=255)                                                                                                                                        ttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTtaggc         >  1:1030386‑M2/9‑146 (MQ=255)                                                                                                                                          gcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgtt       <  2:950067‑M2/142‑1 (MQ=255)                                                                                                                                             tccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGATCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgttgtt    >  1:950132‑M2/4‑148 (MQ=255)                                                                                                                                               cCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTa  >  2:950102‑M2/2‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                   TTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTA  >  U00096/3153359‑3153648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑