Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096380,0130G.22.6% 84.3 / 21.8 32intergenic (‑132/+56)frmR/yaiODNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (22/2);  new base . (4/3);  total (26/6)
Fisher's exact test for biased strand distribution p-value = 6.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTT  >  U00096/379869‑380143
                                                                                                                                                |                                                                                                                                  
gAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                                                 <  1:108582/148‑1 (MQ=255)
      tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCACTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggg                                                                                                                           >  1:108594/1‑148 (MQ=255)
        ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTTGTCGGAAAACAAAGAGGAAAggtgggggggat                                                                                                                         >  1:108617/1‑146 (MQ=255)
            tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATc                                                                                                                     >  1:108597/1‑148 (MQ=255)
            tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                                                                                                                    <  2:108584/148‑1 (MQ=255)
              tCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCgg                                                                                                                   >  2:108604/1‑148 (MQ=255)
                gCACCTCATCATCTGCATGCAATATACTATTGGGGGGTTTTCTTTATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTTAATGTGGTCGGAAAACAATGAGGAAA‑GGGGGGGGGCTAATCGGCAg                                                                                                                <  2:108585/148‑1 (MQ=255)
                 cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAg                                                                                                                >  2:108602/1‑148 (MQ=255)
                 cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAg                                                                                                                >  2:108629/1‑148 (MQ=255)
                       atcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                          >  2:108611/1‑148 (MQ=255)
                            cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                                                     >  2:108635/1‑148 (MQ=255)
                              gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAATACAAAGAGGAAAGGGGGGGGGGCTATTCGGCAGGGAAGGCCGcccc                                                                                                   >  2:108622/1‑148 (MQ=255)
                               catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGAGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                  >  2:108589/1‑148 (MQ=255)
                                  gcaaactaCTATAGGGCGGTATTCTATTTGTCAATGCATACCCCCCTATAGTATAAAACACTGGCGACTAAACATCCCCCGGCGCCCCGGGCCCGCACACCACGCCCCCCGGGCGGGCGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                                                               <  2:108586/142‑1 (MQ=255)
                                        taCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCTGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc                                                                                        >  2:108626/1‑148 (MQ=255)
                                              tctgttgTATTCTAAATGTCAACTCCAACCCCCCTATACTATATAACCCTTCAGAATAAAATTTATCCGGCGCATGTCGCCCCCAAACAACCACGAACCGGGTGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                      <  2:1375228/139‑1 (MQ=255)
                                                       ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCAGAAGGAATCa                                                                          >  2:108607/1‑148 (MQ=255)
                                                         cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGAGGAAGGAATc                                                                           >  2:108619/1‑145 (MQ=255)
                                                           atatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                                     >  1:108623/1‑133 (MQ=255)
                                                                     gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGACGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                            >  1:108631/1‑147 (MQ=255)
                                                                             cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                                                           >  1:108614/1‑125 (MQ=255)
                                                                             cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                    >  1:108613/1‑148 (MQ=255)
                                                                               cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                  >  2:108618/1‑148 (MQ=255)
                                                                               cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                  >  1:108615/1‑147 (MQ=255)
                                                                                 ataGTATATAACACTGCCCAAAAAACTTTATCCGGCGAAGGTGGTCGGAAAACACCGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                               <  2:108588/148‑1 (MQ=255)
                                                                                    gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                            >  1:108638/1‑148 (MQ=255)
                                                                                     tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                            >  1:108621/1‑148 (MQ=255)
                                                                                          aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                            >  1:108612/1‑143 (MQ=255)
                                                                                           acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCGAATCGGCAGGGAAGGCCGCCCCGGATCGGGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGCCGGGCTGCAAGTTGCAGAcc                                      >  2:332493/1‑148 (MQ=255)
                                                                                                    aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAACGAGGAAAGGGGGGAGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc                             <  1:108589/148‑1 (MQ=255)
                                                                                                    aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                          >  1:108632/1‑135 (MQ=255)
                                                                                                          aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGTAATCGGCCGGGAGGGCCGCCCCGGGTAGCGGGCGGGGGAGGGAATCAGAATTTCCAGGTCTGACGGGCTGCAAGTGGCGGCCCGTTAAAATCATCGGt                       >  2:108625/1‑148 (MQ=255)
                                                                                                                      aaTGTGGTCGGAAAACAAAGAGGAAAgggggggggg                                                                                                                           >  1:239668/1‑36 (MQ=255)
                                                                                                                            gTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGACGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAGCAGAATTTCCAGGTCAGACGGGCTGCAAGTGGCAGCCCGGTAAAATCTTGGGTTGGGGTGTCGGACCacac     >  2:108620/1‑148 (MQ=255)
                                                                                                                              cGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt   >  2:108643/1‑148 (MQ=255)
                                                                                                                               ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGCCAGAAGGAATCAGAATTACCAGGTCAGACGGGCTGCAAGTTGCACACCGGTAAAATCAGCGGTTGGGGTGTCGTACCACACttt  >  1:373639/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                  
GAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTT  >  U00096/379869‑380143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑