Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	1,307,934	C→T	G238S (GGC→AGC)	clsA ←	cardiolipin synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	1,307,934	0	C	T	100.0%	197.8 / NA	55	G238S (GGC→AGC)	clsA	cardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/21);  total (34/21)

CGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCT  >  U00096/1307793‑1308078                                                                                                                                              |                                                                                                                                                 cGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGgtggt                                                                                                                                            >  2:1405050/1‑146 (MQ=255)  ggTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTAc                                                                                                                                           <  2:381870/148‑1 (MQ=255)   gTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTAcg                                                                                                                                          >  1:381892/1‑148 (MQ=255)       cAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGAt                                                                                                                                      >  2:381883/1‑148 (MQ=255)         aTCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGt                                                                                                                                    >  2:381906/1‑148 (MQ=255)             cccAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGt                                                                                                                                    <  1:381871/144‑1 (MQ=255)                aGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTAt                                                                                                                              >  1:381886/1‑147 (MQ=255)                    gCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCga                                                                                                                           >  1:381912/1‑146 (MQ=255)                     cAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatct                                                                                                                        >  2:761119/1‑147 (MQ=255)                      aGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatcat                                                                                                                       <  1:381872/148‑1 (MQ=255)                        gAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatcatga                                                                                                                     <  2:381873/148‑1 (MQ=255)                         aataaataATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatcatgat                                                                                                                    <  2:381874/148‑1 (MQ=255)                         aataaataATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatcatgat                                                                                                                    <  1:381875/148‑1 (MQ=255)                          ataaataaTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatcatgatc                                                                                                                   <  2:381876/148‑1 (MQ=255)                            aaataaTCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCgatcatgatcat                                                                                                                 <  1:381877/148‑1 (MQ=255)                              ataatCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCt                                                                                                               <  1:416365/148‑1 (MQ=255)                               taatCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCCTGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCt                                                                                                               >  1:381900/1‑147 (MQ=255)                                      cATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGt                                                                                                       <  2:381878/148‑1 (MQ=255)                                          gcgGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGgc                                                                                                   >  1:381890/1‑148 (MQ=255)                                            ggTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGAGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGgcgc                                                                                                 <  1:381879/148‑1 (MQ=255)                                                  gATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTcc                                                                                           <  1:381880/148‑1 (MQ=255)                                                   aTAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCa                                                                                          >  1:381909/1‑148 (MQ=255)                                                     aGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCCTa                                                                                        >  2:1396248/1‑148 (MQ=255)                                                            tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGt                                                                                  >  1:381902/1‑147 (MQ=255)                                                            tCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTa                                                                                 >  1:381903/1‑148 (MQ=255)                                                               aTACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTaaaa                                                                              >  2:381891/1‑148 (MQ=255)                                                                   gcgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacac                                                                          >  1:381926/1‑148 (MQ=255)                                                                    cgcCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAacac                                                                          <  1:381881/147‑1 (MQ=255)                                                                         tCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCAt                                                                    <  1:381882/148‑1 (MQ=255)                                                                            gATCAATCCATTGCCCTCCGCCCGCAGCTTGTTTGAAGTAGCGAGGATCGACCAGATTCAAGCTGCTGGTGTACGAGATGTAATTATCGATCATGTTCATCTTGAGATGTTGGCGCAGGTCCATACGGCGTTAAAGCACCCGCATCAg                                                                 >  2:381946/1‑148 (MQ=255)                                                                             aTCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAg                                                                 <  1:381883/147‑1 (MQ=255)                                                                               cAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGAtt                                                              >  2:381922/1‑148 (MQ=255)                                                                                aaTCAATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGAAGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTg                                                             >  2:381884/1‑148 (MQ=255)                                                                                   ccATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCCATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGAc                                                           <  1:381885/147‑1 (MQ=255)                                                                                      ttGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCttt                                                       <  2:381886/148‑1 (MQ=255)                                                                                       tGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTa                                                      >  2:381924/1‑148 (MQ=255)                                                                                         cccTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGTCCTTTAAg                                                    <  2:381887/148‑1 (MQ=255)                                                                                             aCGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCtt                                                <  2:381888/148‑1 (MQ=255)                                                                                                cccGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTc                                               >  1:381895/1‑146 (MQ=255)                                                                                                cccGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGa                                             >  2:381904/1‑148 (MQ=255)                                                                                                 ccGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGAc                                            >  2:381923/1‑148 (MQ=255)                                                                                                   gCATCTTGTGTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGCTCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCAGCAGATTGACCTTAAAGGCTTCGACCa                                          >  2:381901/1‑148 (MQ=255)                                                                                                       cTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTc                                      >  1:381897/1‑148 (MQ=255)                                                                                                                     cGAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTAc                        >  1:381899/1‑148 (MQ=255)                                                                                                                      gAGGATCGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACg                       <  2:381889/148‑1 (MQ=255)                                                                                                                            cGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATAc                                 >  1:381929/1‑132 (MQ=255)                                                                                                                            cGACCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAg                 >  1:381905/1‑148 (MQ=255)                                                                                                                              aCCATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCt               >  2:381932/1‑148 (MQ=255)                                                                                                                              aCCATATTCATGCTGCTGGTGTACGCGATGTAATTATCAATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCt               >  2:381941/1‑148 (MQ=255)                                                                                                                               ccATATTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTc              >  2:381935/1‑148 (MQ=255)                                                                                                                                 atatTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAACCACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCgg            >  2:381920/1‑148 (MQ=255)                                                                                                                                   atTCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGc          >  1:381916/1‑148 (MQ=255)                                                                                                                                     tCATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCa        >  2:381911/1‑148 (MQ=255)                                                                                                                                      cATGCTGCTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCAc       <  2:381890/148‑1 (MQ=255)                                                                                                                                          ctgctgGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGc          >  1:381921/1‑141 (MQ=255)                                                                                                                                          ctgctgGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGc   <  1:381891/148‑1 (MQ=255)                                                                                                                                           tgcTGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCt  >  1:381939/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                                 CGGTTTCAATCTCCCAGTCGCAGGAATAAATAATCCCCATCGCGGTGGCGATAGGGCCTTCCATACGCGCCATCAGATCAATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGATCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCT  >  U00096/1307793‑1308078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑