New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	781577 =	51 (0.530)	44 (0.460) +G	6/290	12.2	55.9%	noncoding (1/76 nt)	lysQ	tRNA‑Lys
	?	U00096	= 2521129	19 (0.200)					intergenic (+3/‑44)	valX/valY	tRNA‑Val/tRNA‑Val
Rejected: Coverage evenness skew score above cutoff.

CTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTT  >  U00096/781430‑781723                                                                                                                                                    |                                                                                                                                                   cTGCACGACCCACCAATTTAAAGGAGGTAACTGGAAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATACTGAATGAGTAAGGGAGCATAATCCCGCAAggg                                                                                                                                                   >  2:251898/1‑149 (MQ=25)     aCGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCg                                                                                                                                               >  2:251873/1‑149 (MQ=255)      cGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGt                                                                                                                                              <  2:251834/149‑1 (MQ=255)       gACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGtt                                                                                                                                             >  2:251874/1‑149 (MQ=255)       gACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGtt                                                                                                                                             >  2:251851/1‑149 (MQ=255)        aCCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGGCGTTa                                                                                                                                            >  2:251850/1‑149 (MQ=255)           caccaATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGtt                                                                                                                                             >  1:251843/1‑145 (MQ=255)               aaTTTAACGGTGGTTACTGGTAGAGACCGGGCAGGATAACTTTGCGGTAGCAACGGCCCGATGGGCGAGACGTAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGt                                                                                                                                     <  1:251835/149‑1 (MQ=37)               aaTTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCGCAGt                                                                                                                                     >  2:251854/1‑149 (MQ=255)                         tggtTACTGGTAGAGAACGTGAAGGATACCGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGCCCCACCCTCCTGAATGCGTAAGGCAGCATCAGCCCGCAAGGGGTCGGTAGCTCAGGTGGTAgcgcc                                                                                                                           >  2:251884/1‑148 (MQ=21)                         tggtTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCa                                                                                                                           >  1:251890/1‑149 (MQ=255)                           gtTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                                                                                                         >  2:251882/1‑149 (MQ=255)                                  gTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                                  <  2:251837/149‑1 (MQ=255)                                  gTAGAGAACGTGAAGGATAACGTTGCGTTAGCAAAGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                                                                                                                  >  2:251853/1‑149 (MQ=255)                                      agaACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt                                                                                                              >  2:251892/1‑149 (MQ=255)                                          cGTGAGGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                                                                                                          <  1:251839/149‑1 (MQ=255)                                                      cGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                              >  2:251852/1‑149 (MQ=255)                                                       gTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGCCCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc                                                                                             >  1:251869/1‑149 (MQ=255)                                                        ttGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg                                                                                            >  2:721166/1‑149 (MQ=255)                                                          gCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGt                                                                                                                                                 <  1:251841/94‑1 (MQ=255)                                                               aGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGAAGCATAATCCCTCAAGGGGTCGTTAGCTAAGTTGGTAGAGCAGTTGACTTGTAATCAATTGGTCGCAGGTTCGATTCCTg                                                                                     >  2:251924/1‑149 (MQ=37)                                                                                    aCGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTaaaaa                                                                <  2:251843/149‑1 (MQ=255)                                                                                      gAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAg                                                              >  2:251883/1‑149 (MQ=255)                                                                                      gAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAg                                                              >  2:251900/1‑149 (MQ=255)                                                                                           cGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGccc                                                         >  2:251901/1‑149 (MQ=255)                                                                                              gTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa                                                      >  1:251880/1‑149 (MQ=255)                                                                                                cATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                    >  1:251878/1‑149 (MQ=255)                                                                                                 aTCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                   >  2:251922/1‑149 (MQ=255)                                                                                                  tCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc                                                  >  1:251902/1‑149 (MQ=255)                                                                                                  tCCTGCACGACCCACCATACAGAATGAATAATGCAGCTTAATCCCGCACTGGTTCGTTAGCTCATCTGTTAGATCAGTTGTCTTTTAATCTATAGGTCTCAGCTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgc                                                  <  2:251847/149‑1 (MQ=17)                                                                                                    cTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcgc                                                <  2:251848/149‑1 (MQ=255)                                                                                                           aCCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTTGCTCAGTTGGTAGAGCAGTTGTCTATTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTg                                         <  2:251849/149‑1 (MQ=255)                                                                                                           aCCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTg                                         >  2:251907/1‑149 (MQ=255)                                                                                                                ccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATc                                    <  1:251850/149‑1 (MQ=255)                                                                                                                     cTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGa                               >  2:251910/1‑149 (MQ=255)                                                                                                                       gAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATa                             <  1:251851/149‑1 (MQ=255)                                                                                                                       gAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATa                             >  1:251954/1‑149 (MQ=255)                                                                                                                          tGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTc                          >  1:251896/1‑149 (MQ=255)                                                                                                                            aTTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCaa                        >  2:505200/1‑149 (MQ=255)                                                                                                                             ttAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa                       <  1:251852/149‑1 (MQ=255)                                                                                                                                aGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaa                        >  2:251923/1‑145 (MQ=255)                                                                                                                                  gcagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTc                  <  1:251853/149‑1 (MQ=255)                                                                                                                                    agcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGa                >  2:251897/1‑149 (MQ=255)                                                                                                                                     gcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa               >  2:251908/1‑149 (MQ=255)                                                                                                                                       aTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGAtt                   <  1:251854/143‑1 (MQ=255)                                                                                                                                        tAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCGTTTTTGCTATCTGCGATACTCAAAGATTCGAACCt            >  2:251899/1‑149 (MQ=255)                                                                                                                                           tCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgca         >  2:251905/1‑149 (MQ=39)                                                                                                                                           tCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgc          >  1:251903/1‑148 (MQ=39)                                                                                                                                            cccGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgca         <  1:488225/148‑1 (MQ=38)                                                                                                                                              cGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgcagca      >  1:251912/1‑149 (MQ=37)                                                                                                                                                  aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGtt  >  2:251941/1‑149 (MQ=33)                                                                                                                                                    gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa               <  1:251862/134‑1 (MQ=32)                                                                                                                                                    |                                                                                                                                                   CTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTT  >  U00096/781430‑781723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.