Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	754,123	C→T	E116K (GAG→AAG)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	754,123	0	C	T	100.0%	344.4 / NA	91	E116K (GAG→AAG)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/47);  total (44/47)

GGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGC  >  U00096/753979‑754265                                                                                                                                                 |                                                                                                                                               ggAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTgg                                                                                                                                            >  1:243290/1‑149 (MQ=255)  gAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGa                                                                                                                                           <  2:243241/149‑1 (MQ=255)  gAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGa                                                                                                                                           <  1:243240/149‑1 (MQ=255)   aaCGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGa                                                                                                                                           <  1:243243/148‑1 (MQ=255)   aaCGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGAt                                                                                                                                          <  1:243242/149‑1 (MQ=255)         gCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGt                                                                                                                                    >  2:243294/1‑149 (MQ=255)         gCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGt                                                                                                                                    >  2:243307/1‑149 (MQ=255)          cAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTa                                                                                                                                   <  1:243245/149‑1 (MQ=255)          cAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTa                                                                                                                                   <  1:243246/149‑1 (MQ=255)           aaTTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGt                                                                                                                                    <  1:243247/147‑1 (MQ=255)           aaTTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTAt                                                                                                                                  >  2:243273/1‑149 (MQ=255)             tttCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGa                                                                                                                                >  2:243264/1‑149 (MQ=255)               tCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACg                                                                                                                              >  2:243310/1‑149 (MQ=255)                   ggTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTc                                                                                                                          >  2:243317/1‑149 (MQ=255)                        cGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGt                                                                                                                     >  1:243284/1‑149 (MQ=255)                         gAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTa                                                                                                                    >  1:243285/1‑149 (MQ=255)                         gAGGATTGTTAACATCCAGCGAGGCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTa                                                                                                                    <  1:243248/149‑1 (MQ=255)                              ttGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGtttt                                                                                                               >  1:243275/1‑149 (MQ=255)                               tGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTa                                                                                                              >  2:243277/1‑149 (MQ=255)                               tGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTa                                                                                                              <  2:243250/149‑1 (MQ=255)                               tGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTa                                                                                                              <  2:243249/149‑1 (MQ=255)                               tGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTa                                                                                                              >  2:243305/1‑149 (MQ=255)                               tGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTa                                                                                                              >  1:243312/1‑149 (MQ=255)                                gTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTaa                                                                                                             <  2:243251/149‑1 (MQ=255)                                  tAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAAt                                                                                                           >  2:243315/1‑149 (MQ=255)                                   aaCATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAAAAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAAtt                                                                                                          >  1:243309/1‑149 (MQ=255)                                    aCATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTc                                                                                                         <  2:718310/149‑1 (MQ=255)                                      aTCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGt                                                                                                       <  1:243252/149‑1 (MQ=255)                                       tCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTaaa                                                                                                            >  1:243281/1‑143 (MQ=255)                                        ccAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCa                                                                                                     <  1:243253/149‑1 (MQ=255)                                        ccAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCa                                                                                                     <  1:243254/149‑1 (MQ=255)                                          aGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATTACGGGTCACCGTAGTATTAAATTCGTCATa                                                                                                   >  2:243313/1‑149 (MQ=255)                                          aGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATa                                                                                                   <  1:243255/149‑1 (MQ=255)                                          aGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATa                                                                                                   <  1:243256/149‑1 (MQ=255)                                                 cGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTcc                                                                                            <  1:243257/149‑1 (MQ=255)                                                   tGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTg                                                                                          >  1:243300/1‑149 (MQ=255)                                                    gATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGa                                                                                         >  1:243329/1‑149 (MQ=255)                                                     aTAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAg                                                                                        >  1:243297/1‑149 (MQ=255)                                                     aTAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAg                                                                                        <  2:243258/149‑1 (MQ=255)                                                         aaCGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCgg                                                                                    <  2:243260/149‑1 (MQ=255)                                                         aaCGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCgg                                                                                    <  2:243259/149‑1 (MQ=255)                                                               gccAGCGCGCCGGTAATACCACCCATTACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTTCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTc                                                                              >  1:243283/1‑149 (MQ=255)                                                                    cgcgcCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAt                                                                         <  2:243261/149‑1 (MQ=255)                                                                     gcgcCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCAtt                                                                        >  1:243289/1‑149 (MQ=255)                                                                      cgcCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTc                                                                       <  2:243262/149‑1 (MQ=255)                                                                        ccGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTc                                                                       <  1:243263/147‑1 (MQ=255)                                                                         cGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagc                                                                    >  2:243322/1‑149 (MQ=255)                                                                         cGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagc                                                                    <  1:243264/149‑1 (MQ=255)                                                                          ggTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagca                                                                   >  1:243301/1‑149 (MQ=255)                                                                          ggTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagca                                                                   >  1:243331/1‑149 (MQ=255)                                                                          ggTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTcagca                                                                   >  1:243303/1‑149 (MQ=255)                                                                           gTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTca                                                                      <  1:243265/145‑1 (MQ=255)                                                                             aaTACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGa                                                                <  2:243266/149‑1 (MQ=255)                                                                               tACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATg                                                              <  1:243267/149‑1 (MQ=255)                                                                                  cacacaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTaa                                                           <  2:243268/149‑1 (MQ=255)                                                                                   acacaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTa                                                            <  1:243270/147‑1 (MQ=255)                                                                                   acacaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAAc                                                          <  2:243269/149‑1 (MQ=255)                                                                                        tGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACt                                                     >  2:243296/1‑149 (MQ=255)                                                                                        tGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACt                                                     >  2:243295/1‑149 (MQ=255)                                                                                        tGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACt                                                     <  1:243271/149‑1 (MQ=255)                                                                                          aCTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGATTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTc                                                   <  2:243272/149‑1 (MQ=255)                                                                                           cTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTc                                                   <  1:243273/148‑1 (MQ=255)                                                                                              ccATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAgg                                               >  2:243314/1‑149 (MQ=255)                                                                                                  tGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGt                                           <  1:243274/149‑1 (MQ=255)                                                                                                    gATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTa                                         <  2:369869/149‑1 (MQ=255)                                                                                                       gCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGaa                                      <  2:243275/149‑1 (MQ=255)                                                                                                       gCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGaa                                      >  2:243286/1‑149 (MQ=255)                                                                                                         gAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATc                                    >  1:243320/1‑149 (MQ=255)                                                                                                          aGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGCAACAAACTTCCAGGTAGTTAGAATc                                    <  1:243276/148‑1 (MQ=255)                                                                                                                gACGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGcc                             >  2:243304/1‑149 (MQ=255)                                                                                                                 aCGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCa                            <  1:243277/149‑1 (MQ=255)                                                                                                                  cGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg                           >  1:243318/1‑149 (MQ=255)                                                                                                                  cGGAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCATCATACTGTTCCTGAGGCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg                           <  1:243278/149‑1 (MQ=255)                                                                                                                    gAAAGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACATACTTCCAGGTAGTTAGAATCGGTCGCCCGCt                         >  2:243342/1‑149 (MQ=255)                                                                                                                     aaaGCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTg                        >  2:243311/1‑149 (MQ=255)                                                                                                                        gCATGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatc                     <  2:243279/149‑1 (MQ=255)                                                                                                                          aTGGAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga                   >  1:243328/1‑149 (MQ=255)                                                                                                                             gAACAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAg                           >  1:243287/1‑138 (MQ=255)                                                                                                                              aaCAGACGGGTAATCTGCTTGTGGATCATGGTAAGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCgg               >  1:243323/1‑149 (MQ=255)                                                                                                                               aCAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCggg              >  2:243350/1‑149 (MQ=255)                                                                                                                                cAGACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGa             <  1:243280/149‑1 (MQ=255)                                                                                                                                  gACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGaaa           >  2:243351/1‑149 (MQ=255)                                                                                                                                  gACGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGaaa           >  2:243336/1‑149 (MQ=255)                                                                                                                                   aCGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAAc          >  2:243356/1‑149 (MQ=255)                                                                                                                                   aCGGGTAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAAc          <  2:243281/149‑1 (MQ=255)                                                                                                                                        tAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGa             <  1:243282/141‑1 (MQ=255)                                                                                                                                        tAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGa             >  2:243282/1‑141 (MQ=255)                                                                                                                                        tAATCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCgg     <  2:243283/149‑1 (MQ=255)                                                                                                                                         aaTCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGt    <  2:243284/149‑1 (MQ=255)                                                                                                                                           tCTGCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgc  >  2:243327/1‑149 (MQ=255)                                                                                                                                              gCTTGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgc  <  2:243285/146‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               GGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGC  >  U00096/753979‑754265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑