Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	1,618,245	A→T	*222K (TAA→AAA)	marC ←	inner membrane protein MarC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	1,618,245	0	A	T	100.0%	295.0 / NA	77	*222K (TAA→AAA)	marC	inner membrane protein MarC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (39/38);  total (39/38)

CACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGG  >  U00096/1618102‑1618387                                                                                                                                                |                                                                                                                                               cACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGt                                                                                                                                           >  1:518588/1‑149 (MQ=255)   cTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTAc                                                                                                                                         >  1:518592/1‑149 (MQ=255)    tGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACg                                                                                                                                        <  1:518560/149‑1 (MQ=255)         aaTGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTa                                                                                                                                   >  2:518610/1‑149 (MQ=255)                ttgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGAttt                                                                                                                            <  1:518561/148‑1 (MQ=255)                atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACCCTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGAt                                                                                                                              >  1:518590/1‑147 (MQ=255)                 tgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTc                                                                                                                           <  1:518562/149‑1 (MQ=255)                   atTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCa                                                                                                                         <  1:518563/149‑1 (MQ=255)                   atTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCa                                                                                                                         <  1:518564/149‑1 (MQ=255)                       gTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAg                                                                                                                        <  1:518565/146‑1 (MQ=255)                          aTGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  <  1:518567/149‑1 (MQ=255)                          aTGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGcc                                                                                                                  <  2:518566/149‑1 (MQ=255)                           tgtgGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGCCACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCg                                                                                                                 >  2:518580/1‑149 (MQ=255)                               ggcgcggcgcCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaa                                                                                                             <  2:518568/149‑1 (MQ=255)                                  gcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataa                                                                                                          >  2:518585/1‑149 (MQ=255)                                     gTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCACTAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataataa                                                                                                       <  1:518570/149‑1 (MQ=255)                                     gTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataataa                                                                                                       <  2:518569/149‑1 (MQ=255)                                      tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGGTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATaaa                                                                                                      <  2:518571/149‑1 (MQ=255)                                      tGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATCTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATaaa                                                                                                      <  2:518572/149‑1 (MQ=255)                                        ccTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACt                                                                                                    >  2:518595/1‑149 (MQ=255)                                        ccTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACt                                                                                                    <  2:518573/149‑1 (MQ=255)                                        ccTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACt                                                                                                    <  2:518574/149‑1 (MQ=255)                                          tGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                                                                                  >  2:518596/1‑149 (MQ=255)                                          tGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                                                                                  >  2:518607/1‑149 (MQ=255)                                          tGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGc                                                                                                  >  2:518606/1‑149 (MQ=255)                                            cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcac                                                                                                <  2:518575/149‑1 (MQ=255)                                            cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcac                                                                                                <  1:518577/149‑1 (MQ=255)                                            cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcac                                                                                                >  1:518609/1‑149 (MQ=255)                                            cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAAAAGACGCAATAGTTGAAAGTCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaat                                                                                                            <  1:518576/137‑1 (MQ=255)                                             ttttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                                                                                               >  2:518613/1‑149 (MQ=255)                                              tttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                                                                                              >  1:518614/1‑149 (MQ=255)                                               ttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataataa                                                                                                       >  1:518594/1‑139 (MQ=255)                                               ttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                                                 >  1:518602/1‑145 (MQ=255)                                                    gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcatac                                                                                        >  1:518632/1‑149 (MQ=255)                                                     cgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcataca                                                                                       <  2:518578/149‑1 (MQ=255)                                                        tAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCcata                                                                                         <  1:518579/144‑1 (MQ=255)                                                                tCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGCCGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGCTGCCGTTAATAATAAACTGCACACCCATTCATACCAGCAGGa                                                                            >  2:518636/1‑149 (MQ=255)                                                                tCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGa                                                                            >  1:518626/1‑149 (MQ=255)                                                                 cAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGaa                                                                           >  2:518624/1‑149 (MQ=255)                                                                 cAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGa                                                                            <  1:518580/148‑1 (MQ=255)                                                                 cAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAAGGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGaa                                                                           >  2:518611/1‑149 (MQ=255)                                                                    tCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATcc                                                                        <  1:518581/149‑1 (MQ=255)                                                                      aTTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa                                                                      <  2:518582/149‑1 (MQ=255)                                                                       ttATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt                                                                     >  1:518628/1‑149 (MQ=255)                                                                           atTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCGATCAGa                                                                 <  2:1191951/149‑1 (MQ=255)                                                                           atTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt                                                                     >  1:518599/1‑145 (MQ=255)                                                                               cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACggg                                                             <  2:518583/149‑1 (MQ=255)                                                                                gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGga                                                            <  1:518584/149‑1 (MQ=255)                                                                                gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGga                                                            >  1:518619/1‑149 (MQ=255)                                                                                gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAAAAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGga                                                            >  1:518612/1‑149 (MQ=255)                                                                                 ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGgag                                                           <  1:518585/149‑1 (MQ=255)                                                                                 ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGgag                                                           >  1:518600/1‑149 (MQ=255)                                                                                 ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGgag                                                           >  1:518601/1‑149 (MQ=255)                                                                                 ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGgag                                                           >  2:518629/1‑149 (MQ=255)                                                                                      ggCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGc                                                      >  1:518631/1‑149 (MQ=255)                                                                                         cAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTc                                                   >  1:518620/1‑149 (MQ=255)                                                                                            tGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAAt                                                >  2:518605/1‑149 (MQ=255)                                                                                             gACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACTGGAGATCGCTTc                                                   <  1:518586/145‑1 (MQ=255)                                                                                               cacTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGcc                                             <  1:518587/149‑1 (MQ=255)                                                                                                 cTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAc                                           >  2:518623/1‑149 (MQ=255)                                                                                                    gaagaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCCCACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGcc                                             >  1:518617/1‑144 (MQ=255)                                                                                                    gaagaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCt                                        >  1:518618/1‑149 (MQ=255)                                                                                                       gaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGc                                     <  2:518588/149‑1 (MQ=255)                                                                                                         aCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGccc                                   >  2:518616/1‑149 (MQ=255)                                                                                                           aGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCccac                                 <  2:518589/149‑1 (MQ=255)                                                                                                             aCGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCccacca                               >  1:518639/1‑149 (MQ=255)                                                                                                                cAATAGTTGAAAGGCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGc                             <  1:1129962/148‑1 (MQ=255)                                                                                                                     gTTGAACGGCCCATTCGGGCCGTTTTTTATGGTACGTGTTAATGATTTCCAGGATGCCGGTAATAATAACCTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGAGCGCTTCAATGCCACCCTTGCCCACCAGCCGCATa                       <  2:518590/149‑1 (MQ=255)                                                                                                                        gAAAGGCCCAGTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAGCAGCCGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAAtt                    >  1:518621/1‑149 (MQ=255)                                                                                                                            ggCCCATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTgcgc                <  1:518591/149‑1 (MQ=255)                                                                                                                                cATTCGGGCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGa            <  1:900528/149‑1 (MQ=255)                                                                                                                                     gggCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGAAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGc       >  2:518630/1‑149 (MQ=255)                                                                                                                                       gCCTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCAATCAGACGGGAGATCGCTTCAATGCCACCCTTGCACACCAGCCGCATAATTGCGCAGGAGCTGCGt     >  1:518627/1‑149 (MQ=255)                                                                                                                                        ccTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTa    <  2:518594/149‑1 (MQ=255)                                                                                                                                        ccTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTa    <  2:518592/149‑1 (MQ=255)                                                                                                                                        ccTTTTTTTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTa    <  1:518593/149‑1 (MQ=255)                                                                                                                                          ttttttTATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAgg  >  1:518651/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                               CACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGG  >  U00096/1618102‑1618387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑