Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG25.0% 428.1 / 33.4 198intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (13/134);  new base G (48/1);  total (63/135)
Fisher's exact test for biased strand distribution p-value = 1.84e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 209 total aligned reads displayed.

TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  U00096/422283‑422577
                                                                                                                                                  |                                                                                                                                                    
  gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGgccgc                                                                                                                                                  >  2:399148/1‑147 (MQ=255)
   cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                 <  1:398954/149‑1 (MQ=255)
   cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                 <  2:398953/149‑1 (MQ=255)
     tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGgccgcctc                                                                                                                                               >  2:399077/1‑149 (MQ=255)
         ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                           <  2:398957/149‑1 (MQ=255)
           gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCGCCGCGCCCaa                                                                                                                                         >  2:399113/1‑149 (MQ=255)
            ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCcaa                                                                                                                                        >  2:399093/1‑149 (MQ=255)
            ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGGAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                        <  2:398959/149‑1 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                      <  2:398961/149‑1 (MQ=255)
              gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                      <  1:398962/149‑1 (MQ=255)
                 cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATcc                                                                                                                                   >  2:399045/1‑149 (MQ=255)
                   gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                 <  2:398966/149‑1 (MQ=255)
                     tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCATGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                               <  1:398969/149‑1 (MQ=255)
                       aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                             <  2:398970/149‑1 (MQ=255)
                          ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatga                                                                                                                          <  2:398972/149‑1 (MQ=255)
                            cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                        <  1:398976/149‑1 (MQ=255)
                            cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                        <  2:398974/149‑1 (MQ=255)
                             tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATAACCTTAAGAtgatga                                                                                                                       >  2:399117/1‑149 (MQ=255)
                                aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                    <  1:398979/149‑1 (MQ=255)
                                  gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCCAAGATCCTTCAGAAGAAGAGTgct                                                                                                                  >  2:399098/1‑147 (MQ=255)
                                     tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                               <  1:398986/149‑1 (MQ=255)
                                     tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                               <  1:398984/149‑1 (MQ=255)
                                        aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                            <  2:398987/149‑1 (MQ=255)
                                        aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATACTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                            <  2:398989/149‑1 (MQ=255)
                                          gcctccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCATCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                          <  1:398993/149‑1 (MQ=255)
                                          gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTGCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                          <  2:398992/149‑1 (MQ=255)
                                           ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                    <  1:398997/138‑1 (MQ=255)
                                           ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                         <  2:398996/149‑1 (MQ=255)
                                            cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                        <  2:398999/149‑1 (MQ=255)
                                              ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                     <  2:398964/118‑1 (MQ=255)
                                              ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCGTCAGATGGTGAGTGGTGCGGGATTAggg                                                                                                      >  2:399183/1‑148 (MQ=255)
                                                aCGATCGTCAGCTGGCTGAAAACCAGTAATACTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                    <  1:399002/149‑1 (MQ=255)
                                                 cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                   <  2:399004/149‑1 (MQ=255)
                                                    tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                <  1:399006/149‑1 (MQ=255)
                                                        aaGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                            <  2:399007/148‑1 (MQ=255)
                                                           ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGa                                                                                         <  2:399009/149‑1 (MQ=255)
                                                             ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCCTTAGGCTATGGCAAGGTGATc                                                                                       <  2:399013/149‑1 (MQ=255)
                                                              gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCCAATATCCTTCAGATGATGAGTGTTCCTGCTTTAGGGTTTGGCAAGGTggtga                                                                                      >  2:399136/1‑145 (MQ=255)
                                                              gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                       <  1:399015/148‑1 (MQ=255)
                                                              gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                      <  2:399016/149‑1 (MQ=255)
                                                                  aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                    <  2:398980/99‑1 (MQ=255)
                                                                   aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATCCAAAAGAAGATGAGTGATCCAGCAATAGGGTATGGCAAGGTGATCAGAatt                                                                                 >  2:399104/1‑149 (MQ=255)
                                                                    aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGAtttt                                                                                <  2:399019/149‑1 (MQ=255)
                                                                         gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                           <  2:399022/149‑1 (MQ=255)
                                                                          tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                           <  1:399024/148‑1 (MQ=255)
                                                                           aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                         <  2:399025/149‑1 (MQ=255)
                                                                            aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCAGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGGGGAGTGAGGCGGGATTAGGCGATGGGAAGGGGATCAGATTTTGATGACAg                                                                        >  2:399172/1‑149 (MQ=255)
                                                                             tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                       <  1:399027/149‑1 (MQ=255)
                                                                             tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                       <  1:399029/149‑1 (MQ=255)
                                                                              ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                      <  2:399030/149‑1 (MQ=255)
                                                                                ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                    <  1:399032/149‑1 (MQ=255)
                                                                                  gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                  <  2:399033/149‑1 (MQ=255)
                                                                                    gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                <  2:399036/149‑1 (MQ=255)
                                                                                     ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                               <  1:399038/85‑1 (MQ=255)
                                                                                       cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                             <  1:399040/149‑1 (MQ=255)
                                                                                        tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                            <  2:399042/149‑1 (MQ=255)
                                                                                          tAAGCCGGTTCAGGAGGAAATGCTGATCCGGCTATTCGGAAAGGTATCCGCCCCCGTCCTCCTCCCCCAATTATACTTCAGATGATTAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                          <  1:399044/149‑1 (MQ=255)
                                                                                             gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                        <  1:399047/148‑1 (MQ=255)
                                                                                             gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCGTCAGAGGAGGAGTGATGCTGGAGTAGGGGATGGCAAGGGGGTCAGATTTTAGTGACAGGGGAGTTATGATGTTaa                                                       >  2:399194/1‑149 (MQ=255)
                                                                                                ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCCGCGCCCAAAGATCCAACAGAAGATGAGTGATCCTGCAGTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAAATATGATGTTAAAtt                                                    >  2:399155/1‑148 (MQ=255)
                                                                                                ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATACGCCCGCGAGCGGCGCGCCCGAGTAACCGTCCGAAGATGAGTGCTCAGGCGTTAGGAGAATGCAAGTTGCTAAGATATTGATAACGGGGGAATTGAGTTGTGAAATg                                                    >  2:399158/1‑149 (MQ=255)
                                                                                                 gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCTCCCCCAAAGATCCGTCAGATGAAGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGGATTATGAGGTTTAATGc                                                   >  2:399048/1‑149 (MQ=255)
                                                                                                  ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                  <  2:399053/149‑1 (MQ=255)
                                                                                                  ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                  <  1:399052/149‑1 (MQ=255)
                                                                                                         ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                           <  2:399056/149‑1 (MQ=255)
                                                                                                           aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                             <  1:399057/145‑1 (MQ=255)
                                                                                                             aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTCCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGcc                                       <  2:399059/149‑1 (MQ=255)
                                                                                                              tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAAAATACTTCAGATGATGAGTGCTGGAGCAATAGGCGATGGGAAGGTGATCAGAATTTCATGACAGGGGAAGTATGATGTTAAATGCATGGCACCAGCCg                                      >  2:399141/1‑149 (MQ=255)
                                                                                                               ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                     <  1:399061/149‑1 (MQ=255)
                                                                                                                 tGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCACCCCCCCCAAATATACTTCAGATCATGAGTGAGCCGGCATTAGGCTAAGGGAAGGGGATCAGAGTTTCATGACAGGGGTTTTATGATGTTAGATGAATGGCACCTGCCgggg                                   >  2:399231/1‑147 (MQ=255)
                                                                                                                 tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCAGTAGGGTATGGGAAGGTGGTGAGATTTTGATATAAGGGGTATTATTATGTTAAATGAATGGCACCTGCCgggg                                   >  2:399124/1‑147 (MQ=255)
                                                                                                                    tCCGGCTATTCCGAAAGTTATCCGGGGCCGGCCGCCGCCCCCCAATATCCGTCAGATGATGAGTGATCCGGCATTAGGGGTTGGCAAGGTGATCCGGGTTTGATAACAGGGGAATGATGATGTTATATGCATTGCACCTGCCgggggcc                                >  2:399162/1‑144 (MQ=255)
                                                                                                                     ccGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCACAAATACATCAGATGAGGAGTGATGCTGCAGAAGGCTATGGCAAGGTGAGCAGATGTTGATAAACGGGGAATTATGAGGTTAAATGCATGGCACAAGCCGGTGcccc                               >  2:399131/1‑149 (MQ=255)
                                                                                                                      cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccc                              <  2:399063/149‑1 (MQ=255)
                                                                                                                         cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAt                            <  1:399065/148‑1 (MQ=255)
                                                                                                                         cTATTCCGAAAGTTATCCGCCCCCGGCCTGCTGCCCCAAAAAAACATAAGATGAAGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCTAGACACGGGAATTATGATGTTAAATGCATTGCACCTGCCGGTGCCCCCagt                           >  2:1809175/1‑147 (MQ=255)
                                                                                                                             tCCGACAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                       <  2:399068/149‑1 (MQ=255)
                                                                                                                             tCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATAATTAAGATGATGAGTGATCCTGCGTTAGGCGATGGGAAGGGGATAAGATTTTAAAAACAGGGGAATTATTATGATTAATGCATGGCACCAGCCCGTGCCCCCCgttgtt                       >  2:399234/1‑149 (MQ=255)
                                                                                                                             tCCGAAAGTTATCCGCCCCCATCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                       <  1:399067/149‑1 (MQ=255)
                                                                                                                               cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                     <  2:399072/149‑1 (MQ=255)
                                                                                                                               cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                     <  2:399070/149‑1 (MQ=255)
                                                                                                                                gAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGATTTTAATCAAAGGGGAATTATGATGTTTAATGGATGGCACCTGCCGGGGCGCCCATGTGTTaaa                    >  2:399238/1‑149 (MQ=255)
                                                                                                                                 aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                        <  1:1945364/64‑1 (MQ=255)
                                                                                                                                  aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                  <  1:399074/149‑1 (MQ=255)
                                                                                                                                    gTTATCCGGCCCCGGCCGCCGCCCCCAAATATACTTGAGATGATGAGTGATCCTGCATTATGCTAGGGCAAGGTGATAAGATTTTAATAAAAGGGGAATTATTATTTTTAATGAATGGCACCTTCCGGGGCCGCCATTTGTTAaaaaaa                >  2:399186/1‑149 (MQ=255)
                                                                                                                                     ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag               <  1:399076/149‑1 (MQ=255)
                                                                                                                                       aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca             <  1:399078/149‑1 (MQ=255)
                                                                                                                                       aTCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATGACAGGGGAATTATGTTGTTTAATGAATGGCACCTGCCGGGGGCCCCAATTGTTTAAaaaagca             >  2:399127/1‑149 (MQ=255)
                                                                                                                                        tCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            <  2:399081/149‑1 (MQ=255)
                                                                                                                                         ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            <  1:399082/148‑1 (MQ=255)
                                                                                                                                          cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag          >  2:399153/1‑149 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa         <  2:399085/149‑1 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa         <  1:399084/149‑1 (MQ=255)
                                                                                                                                           gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa         <  2:399087/149‑1 (MQ=255)
                                                                                                                                            cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            >  2:399217/1‑145 (MQ=255)
                                                                                                                                             ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            >  2:399207/1‑144 (MQ=255)
                                                                                                                                               ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa     >  1:399187/1‑149 (MQ=255)
                                                                                                                                                 gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt   <  1:399089/149‑1 (MQ=255)
                                                                                                                                                  tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTTCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  <  2:399090/149‑1 (MQ=255)
                                                                                                                                                  tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCTAAGATCAACt   >  1:399254/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  U00096/422283‑422577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑