Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 2,661,812 T→A H107L (CAC→CTC)  iscR ← DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000962,661,8120TA100.0% 941.3 / NA 243H107L (CAC→CTC) iscRDNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (135/108);  total (135/108)

Only 100 of 243 total aligned reads displayed.

TCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACG  >  U00096/2661666‑2661957
                                                                                                                                                  |                                                                                                                                                 
tCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAgg                                                                                                                                                 <  1:2435566/149‑1 (MQ=255)
  gcgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGt                                                                                                                                               >  2:2435797/1‑149 (MQ=255)
   cgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTc                                                                                                                                              >  1:2435740/1‑149 (MQ=255)
    gTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGCCGACCAGACACATCCCGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAACCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTc                                                                                                                                              >  1:2435614/1‑148 (MQ=255)
      cTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAgg                                                                                                                                           >  1:2435736/1‑149 (MQ=255)
      cTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAgg                                                                                                                                           >  1:2435618/1‑149 (MQ=255)
        tgtgtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCa                                                                                                                                         <  2:2435568/149‑1 (MQ=255)
           gtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCAttt                                                                                                                                      <  1:2435569/149‑1 (MQ=255)
             gCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCCGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAATCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTAt                                                                                                                                    <  2:2435571/149‑1 (MQ=255)
                 gTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATcgcc                                                                                                                                <  1:2435573/149‑1 (MQ=255)
                     gTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGccc                                                                                                                            <  2:2435575/149‑1 (MQ=255)
                       ggCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTg                                                                                                                          >  1:2435701/1‑149 (MQ=255)
                            gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCAtt                                                                                                                                       >  1:2435706/1‑131 (MQ=255)
                            gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATcgccg                                                                                                                               >  1:2435647/1‑139 (MQ=255)
                            gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgc                                                                                                                     <  2:2435581/149‑1 (MQ=255)
                            gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgc                                                                                                                     >  1:2435816/1‑149 (MQ=255)
                            gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgc                                                                                                                     <  1:2435582/149‑1 (MQ=255)
                             tcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCt                                                                                                                           >  2:2435579/1‑142 (MQ=255)
                              cgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgccg                                                                                                                   <  1:2435585/149‑1 (MQ=255)
                              cgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgccg                                                                                                                   >  2:2435687/1‑149 (MQ=255)
                               gtGAGTATGCTGAGGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgccgc                                                                                                                  >  2:2435768/1‑149 (MQ=255)
                               gtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTAt                                                                                                                                    >  1:2435680/1‑131 (MQ=255)
                               gtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgccgc                                                                                                                  >  1:2435745/1‑149 (MQ=255)
                                tGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAgccgcc                                                                                                                 >  1:2435664/1‑149 (MQ=255)
                                   gTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCa                                                                                                                       <  1:2435591/140‑1 (MQ=255)
                                   gTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCttt                                                                                                              <  2:2435589/149‑1 (MQ=255)
                                    tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTa                                                                                                             >  2:2435620/1‑149 (MQ=255)
                                    tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTa                                                                                                             >  2:2435765/1‑149 (MQ=255)
                                     aTGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAc                                                                                                            <  2:2435593/149‑1 (MQ=255)
                                         tGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTg                                                                                                        >  2:2435715/1‑149 (MQ=255)
                                          gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGa                                                                                                       <  2:2435596/149‑1 (MQ=255)
                                           acgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGAc                                                                                                      <  2:2435598/149‑1 (MQ=255)
                                             gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACaa                                                                                                    >  1:2435718/1‑149 (MQ=255)
                                               ccAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACg                                                                                                  <  1:2435602/149‑1 (MQ=255)
                                                 aGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg                                                                                                >  2:2435814/1‑149 (MQ=255)
                                                  gACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGt                                                                                               >  1:2435716/1‑149 (MQ=255)
                                                   acacATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg                                                                                              <  1:2435606/149‑1 (MQ=255)
                                                   acacATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg                                                                                              <  2:2435607/149‑1 (MQ=255)
                                                           aGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTAc                                                                                      <  2:2435611/149‑1 (MQ=255)
                                                            gCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACa                                                                                     >  1:2435696/1‑149 (MQ=255)
                                                            gCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACa                                                                                     <  2:2435613/149‑1 (MQ=255)
                                                            gCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACa                                                                                     >  1:2435836/1‑149 (MQ=255)
                                                              aCTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTAc                                                                                      >  1:2435720/1‑146 (MQ=255)
                                                                ttCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGAtt                                                                                 <  2:2435617/149‑1 (MQ=255)
                                                                   cTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGt                                                                              >  1:2435755/1‑149 (MQ=255)
                                                                   cTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGt                                                                              >  2:2435751/1‑149 (MQ=255)
                                                                    tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc                                                                             <  1:2435622/149‑1 (MQ=255)
                                                                    tGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc                                                                             >  1:2435732/1‑149 (MQ=255)
                                                                     ggTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCa                                                                            <  2:2435624/149‑1 (MQ=255)
                                                                       ttattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAAc                                                                          <  2:2435628/149‑1 (MQ=255)
                                                                        tattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAAc                                                                          >  1:2435806/1‑148 (MQ=255)
                                                                        tattaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCAGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACg                                                                         <  1:2435629/149‑1 (MQ=255)
                                                                            aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTc                                                                             <  1:2435633/141‑1 (MQ=255)
                                                                             aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCt                                                                    <  2:2435635/149‑1 (MQ=255)
                                                                                aGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAAt                                                                 >  2:2435842/1‑149 (MQ=255)
                                                                                  ttCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTa                                                               >  1:2435764/1‑149 (MQ=255)
                                                                                  ttCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTa                                                               >  2:2435786/1‑149 (MQ=255)
                                                                                  ttCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTa                                                               <  1:2435638/149‑1 (MQ=255)
                                                                                   tCGCCTAAAGTAATGGTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTAc                                                              <  2:2435639/149‑1 (MQ=255)
                                                                                     gCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACtt                                                            >  1:2435711/1‑149 (MQ=255)
                                                                                        tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGc                                                         >  2:2435731/1‑149 (MQ=255)
                                                                                         aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGcc                                                        >  2:2435779/1‑149 (MQ=255)
                                                                                         aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGcc                                                        >  2:2435744/1‑149 (MQ=255)
                                                                                          aaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCa                                                       <  2:2435642/149‑1 (MQ=255)
                                                                                            gTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCCGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                                     >  2:2435834/1‑149 (MQ=255)
                                                                                             tAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACg                                                    >  1:2435753/1‑149 (MQ=255)
                                                                                              aaTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACgg                                                   >  2:2435699/1‑149 (MQ=255)
                                                                                               aTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACg                                                    >  1:2435832/1‑147 (MQ=255)
                                                                                               aTGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGc                                                  <  2:2435645/149‑1 (MQ=255)
                                                                                                tgttgtTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCg                                                 >  2:2435827/1‑149 (MQ=255)
                                                                                                tgttgtTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCg                                                 >  1:2435790/1‑149 (MQ=255)
                                                                                                 gttgttGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGa                                                <  1:2435649/149‑1 (MQ=255)
                                                                                                 ggtgttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGa                                                <  2:2435647/147‑1 (MQ=255)
                                                                                                  ttgttgAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAt                                               >  2:2435749/1‑149 (MQ=255)
                                                                                                       gagaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCaa                                                      <  1:2435652/137‑1 (MQ=255)
                                                                                                       gagaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctgc                                          <  2:2435653/149‑1 (MQ=255)
                                                                                                        agaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATgctgct                                         >  1:2435726/1‑149 (MQ=255)
                                                                                                            aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa                                     >  2:2435738/1‑149 (MQ=255)
                                                                                                            aaCCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGGGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa                                     <  2:2435656/149‑1 (MQ=255)
                                                                                                                 gTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCttt                                <  1:2435659/149‑1 (MQ=255)
                                                                                                                 gTGAGACGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCttt                                >  2:2435658/1‑149 (MQ=255)
                                                                                                                    agaCGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGGATCTTTGcc                             <  1:2435661/149‑1 (MQ=255)
                                                                                                                      aCGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTa                           >  2:2435775/1‑149 (MQ=255)
                                                                                                                       cGGTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa                          <  2:2435664/149‑1 (MQ=255)
                                                                                                                         gTCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa                        <  1:2435666/149‑1 (MQ=255)
                                                                                                                          tCGCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAg                       >  1:2435785/1‑149 (MQ=255)
                                                                                                                            gCTCAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc                         >  1:2435871/1‑145 (MQ=255)
                                                                                                                              taaaaTCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGTGTGGCATATACAGATTAGTCAACGGCGATAATTACTTCGCCAACTGCGATGCTGCTTGAATCTTTGCATAACAGATaa                   >  2:2435896/3‑149 (MQ=255)
                                                                                                                               cAAATCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAac                  >  1:2435856/1‑149 (MQ=255)
                                                                                                                                  aTCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac               <  2:2435668/149‑1 (MQ=255)
                                                                                                                                  aTCACGCCACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAaccac               >  1:2435781/1‑149 (MQ=255)
                                                                                                                                        ccACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCt          >  1:2435798/1‑148 (MQ=255)
                                                                                                                                        ccACAGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTg         <  2:2435671/149‑1 (MQ=255)
                                                                                                                                         cacaGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTgg        <  2:2435673/149‑1 (MQ=255)
                                                                                                                                          acaGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGt       <  1:2435674/149‑1 (MQ=255)
                                                                                                                                            aGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTcc     <  2:2435678/149‑1 (MQ=255)
                                                                                                                                            aGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTcc     >  2:2435802/1‑149 (MQ=255)
                                                                                                                                            aGCGCGAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTcc     <  1:2435677/149‑1 (MQ=255)
                                                                                                                                            aGCGCGAGGGGCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTcc     <  2:2435676/149‑1 (MQ=255)
                                                                                                                                               gcgAGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACg  >  2:2435810/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACG  >  U00096/2661666‑2661957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑