BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	4,033,611	G→A	G156D (GGT→GAT)	trkH →	K(+) transporter TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	4,033,611	0	G	A	100.0%	901.1 / NA	231	G156D (GGT→GAT)	trkH	K(+) transporter TrkH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (113/118); total (113/118)

Only 100 of 241 total aligned reads displayed.

GGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGA  >  U00096/4033466‑4033756

 gCCTGACCACTACGGGAGCCCCTACGCTGGTGGGGCGGGATTCGCTCCCGCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCgtggatgg                                                                                                                                                <  1:3862242/148‑1 (MQ=255)

  ccTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGa                                                                                                                                              <  1:3862245/149‑1 (MQ=255)

  ccTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGa                                                                                                                                              >  2:3862333/1‑149 (MQ=255)

    tGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTggatgggatg                                                                                                                                            >  2:3862246/1‑149 (MQ=255)

        cACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCTGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGc                                                                                                                                        <  2:3862251/149‑1 (MQ=255)

        cACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGc                                                                                                                                        <  2:3862253/149‑1 (MQ=255)

         aCTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGct                                                                                                                                       >  1:3862250/1‑149 (MQ=255)

               ggAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCa                                                                                                                                          >  1:3862435/1‑140 (MQ=255)

                     aCTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCgcgc                                                                                                                              >  1:3862329/1‑146 (MQ=255)

                     aCTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGa                                                                                                                           <  2:3862257/149‑1 (MQ=255)

                       tACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGaaa                                                                                                                         <  2:3862260/149‑1 (MQ=255)

                        aCGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCGCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAAt                                                                                                                        >  2:3862298/1‑149 (MQ=255)

                            tggtggGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATgccc                                                                                                                    >  2:3862262/1‑149 (MQ=255)

                             ggtggGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATgcccg                                                                                                                   <  1:3862266/149‑1 (MQ=255)

                             ggtggGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATgcccg                                                                                                                   <  2:3862267/149‑1 (MQ=255)

                               tggGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATgccc                                                                                                                    <  1:3862269/146‑1 (MQ=255)

                                ggggCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATgcccggcc                                                                                                                >  1:3862468/1‑149 (MQ=255)

                                   gCTGTATACGCTCCCTCACGCCATCCTCTTTTATCGACAGATGCTGAAATGGATTGGCGGGAAGGGCAGCATCGAGTTGGCGGTTGCGATACTGCCTATACTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGc                                                                                                             >  2:3862371/1‑149 (MQ=255)

                                   gCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGc                                                                                                             <  2:3862271/149‑1 (MQ=255)

                                      ggATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGa                                                                                                          >  1:3862451/1‑149 (MQ=255)

                                        aTTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGaaa                                                                                                        <  1:3862276/149‑1 (MQ=255)

                                        aTTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGaaa                                                                                                        <  2:3862277/149‑1 (MQ=255)

                                         ttCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAg                                                                                                       >  1:3862299/1‑149 (MQ=255)

                                          tCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTggatgggatg                                                                                                                                            >  1:3862402/1‑111 (MQ=255)

                                            gCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATa                                                                                                    <  2:3862280/149‑1 (MQ=255)

                                            gCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATa                                                                                                    <  2:3862282/149‑1 (MQ=255)

                                              tCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAAc                                                                                                  >  1:3862334/1‑149 (MQ=255)

                                              tCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAAc                                                                                                  >  2:3862449/1‑149 (MQ=255)

                                              tCCCTCACGCAATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGATCTATCGCGCATCAATGCCCGGCCCGCTGAAAGATAAc                                                                                                  >  2:3862552/1‑149 (MQ=255)

                                               cccTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACa                                                                                                 >  2:3862424/1‑149 (MQ=255)

                                                ccTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaa                                                                                                >  2:3862434/1‑149 (MQ=255)

                                                 cTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaaa                                                                                               >  2:3862441/1‑149 (MQ=255)

                                                 cTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaa                                                                                                <  1:3862285/148‑1 (MQ=255)

                                                  tCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaaaa                                                                                              <  2:3862287/149‑1 (MQ=255)

                                                    aCGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATg                                                                                            <  2:3862289/149‑1 (MQ=255)

                                                       ccATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATgcgc                                                                                         <  1:3862292/149‑1 (MQ=255)

                                                        cATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGcc                                                                                        >  2:3862378/1‑149 (MQ=255)

                                                        cATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGcc                                                                                        <  2:3862293/149‑1 (MQ=255)

                                                        cATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGcc                                                                                        >  2:3862427/1‑149 (MQ=255)

                                                         aTCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGccc                                                                                       >  2:3862387/1‑149 (MQ=255)

                                                          tCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCcg                                                                                      >  2:3862411/1‑149 (MQ=255)

                                                            ctctTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATg                                                                                            >  1:3862470/1‑141 (MQ=255)

                                                             tctTTTATCGCCAGATGGTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGa                                                                                   >  1:3862372/1‑149 (MQ=255)

                                                               ttttATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAt                                                                                 >  1:3862419/1‑149 (MQ=255)

                                                                tttATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAtt                                                                                >  2:3862417/1‑149 (MQ=255)

                                                                 ttATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAAtt                                                                                >  1:3862368/1‑148 (MQ=255)

                                                                  tATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc                                                                              >  1:3862445/1‑149 (MQ=255)

                                                                  tATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGc                                                                              <  1:3862297/149‑1 (MQ=255)

                                                                       ccAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGaaa                                                                         <  1:3862304/149‑1 (MQ=255)

                                                                        cAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAAc                                                                        >  2:3862481/1‑149 (MQ=255)

                                                                        cAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAAc                                                                        <  2:3862305/149‑1 (MQ=255)

                                                                         aGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACg                                                                       >  2:3862465/1‑149 (MQ=255)

                                                                          gATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACgg                                                                      >  2:3862382/1‑149 (MQ=255)

                                                                            tgctgcAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCg                                                                    <  1:3862308/149‑1 (MQ=255)

                                                                               tgcAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGaaa                                                                 <  1:3862310/149‑1 (MQ=255)

                                                                               tgcAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGaaa                                                                 <  2:3862311/149‑1 (MQ=255)

                                                                                 cAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAAc                                                               >  1:3862423/1‑149 (MQ=255)

                                                                                    tGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAAccc                                                             >  1:3862355/1‑148 (MQ=255)

                                                                                    tGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCt                                                            <  1:3862314/149‑1 (MQ=255)

                                                                                     ggTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCtg                                                           <  2:3862317/149‑1 (MQ=255)

                                                                                      gTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCtgt                                                          >  2:3862492/1‑149 (MQ=255)

                                                                                           gCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTg                                                     >  2:3862396/1‑149 (MQ=255)

                                                                                            cGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGAATGAAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGa                                                    <  2:3862320/149‑1 (MQ=255)

                                                                                             gggATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGAt                                                   <  2:3862322/149‑1 (MQ=255)

                                                                                              ggATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCGGTGGTTGAtt                                                  >  2:3862475/1‑149 (MQ=255)

                                                                                               gATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGAttt                                                 >  2:3862432/1‑149 (MQ=255)

                                                                                                aTGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTa                                                <  1:3862323/149‑1 (MQ=255)

                                                                                                   gggATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGt                                             <  2:3862327/149‑1 (MQ=255)

                                                                                                     gATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTg                                                     <  1:3862332/139‑1 (MQ=255)

                                                                                                     gATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCt                                           <  2:3862329/149‑1 (MQ=255)

                                                                                                           cGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGa                                     <  1:3862338/149‑1 (MQ=255)

                                                                                                            gtgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGAc                                    >  2:3862539/1‑149 (MQ=255)

                                                                                                             tgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGa                                     >  1:3862397/1‑147 (MQ=255)

                                                                                                             tgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGAcc                                   <  2:3862339/149‑1 (MQ=255)

                                                                                                             tgtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGAcc                                   <  1:3862341/149‑1 (MQ=255)

                                                                                                              gtTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCg                                  <  2:3862342/149‑1 (MQ=255)

                                                                                                               gtGGCGGTTGCGATACTGCCTATCCTCGGCGTGGATGGTATGCAGCTCTATCGAGCAGAAGTGAGATGCCCGCTGAAAGATATCAAAATGCTCCCTCGAATTTCGGAAACGGCGACAACACGGTGGTTGATTTATGTCTTGc                                        >  1:3862557/2‑142 (MQ=255)

                                                                                                                   cGGTTGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTg                                      >  1:3862457/1‑140 (MQ=255)

                                                                                                                      ttGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACaaaa                                                                                              >  1:3862488/1‑81 (MQ=255)

                                                                                                                      ttGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCtgt                          >  1:3862536/1‑149 (MQ=255)

                                                                                                                      tgtcGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACGCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCtgt                          <  1:3862349/146‑1 (MQ=255)

                                                                                                                       tGCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACTATATGAGCCAGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCtgt                          <  1:3862346/148‑1 (MQ=255)

                                                                                                                        gCGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTgc                        <  2:3862348/149‑1 (MQ=255)

                                                                                                                         cGATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTgcg                       >  1:3862494/1‑149 (MQ=255)

                                                                                                                          gATACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTgcg                       >  1:3862393/1‑148 (MQ=255)

                                                                                                                           aTACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTg                                      <  2:3862352/132‑1 (MQ=255)

                                                                                                                           aTACTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTg                                      >  1:3862352/1‑132 (MQ=255)

                                                                                                                             aCTGCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTgg                   <  2:3862354/149‑1 (MQ=255)

                                                                                                                                gCCTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGt                >  2:3862455/1‑149 (MQ=255)

                                                                                                                                  cTATCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtg              <  1:3862357/149‑1 (MQ=255)

                                                                                                                                    aTCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTTAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtgt             <  1:3862360/148‑1 (MQ=255)

                                                                                                                                    aTCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtgtg            >  2:3862471/1‑149 (MQ=255)

                                                                                                                                    aTCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtgtg            <  2:3862361/149‑1 (MQ=255)

                                                                                                                                    aTCCTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTtgtg            <  2:3862359/149‑1 (MQ=255)

                                                                                                                                      ccTCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGt          >  1:3862362/1‑149 (MQ=255)

                                                                                                                                        tCGGCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGttt        >  2:3862462/1‑149 (MQ=255)

                                                                                                                                          ggCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGc      <  1:3862366/149‑1 (MQ=255)

                                                                                                                                           gCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGcc     <  2:3862367/149‑1 (MQ=255)

                                                                                                                                           gCGTGGATGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGcc     >  2:3862509/1‑149 (MQ=255)

                                                                                                                                              tggatgggATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGa  >  1:3862377/1‑149 (MQ=255)

GGCCTGACCACTACGGGAGCCACTACGCTGGTGGGGCTGGATTCGCTCCCTCACGCCATCCTCTTTTATCGCCAGATGCTGCAATGGTTTGGCGGGATGGGGATCATCGTGTTGGCGGTTGCGATACTGCCTATCCTCGGCGTGGGTGGGATGCAGCTCTATCGCGCAGAAATGCCCGGCCCGCTGAAAGATAACAAAATGCGCCCGCGAATTGCGGAAACGGCGAAAACCCTGTGGTTGATTTATGTCTTGCTGACCGTCGCCTGTGCGCTGGCGTTGTGGTTTGCCGGA  >  U00096/4033466‑4033756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`