Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 4,634,494 C→A G273* (GGA→TGA)  rob ← DNA‑binding transcriptional dual regulator Rob

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000964,634,4940CA99.1% 393.0 / ‑5.2 109G273* (GGA→TGA) robDNA‑binding transcriptional dual regulator Rob
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (51/57);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

Only 100 of 109 total aligned reads displayed.

TCAGGAAAGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCAC  >  U00096/4634348‑4634638
                                                                                                                                                  |                                                                                                                                                
tCAGGAAAGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACg                                                                                                                                                >  1:2071928/1‑149 (MQ=255)
    gAAAGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCttt                                                                                                                                            <  1:2071918/149‑1 (MQ=255)
    gAAAGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCttt                                                                                                                                            >  2:2071964/1‑149 (MQ=255)
    gAAAGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCtt                                                                                                                                             <  1:2071919/148‑1 (MQ=255)
    gAAAGCCTGTGGCTGGCGGCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCttt                                                                                                                                            <  2:2071920/149‑1 (MQ=255)
      aaGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTgg                                                                                                                                          <  1:2071921/149‑1 (MQ=255)
         ccTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAt                                                                                                                                       <  1:2071922/149‑1 (MQ=255)
           tgtgGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAtct                                                                                                                                     >  2:2071955/1‑149 (MQ=255)
           tgtgGCTGGCGTCTGGCGGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAtct                                                                                                                                     <  2:2071923/149‑1 (MQ=255)
            gtgGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAtctt                                                                                                                                    <  1:2071924/149‑1 (MQ=255)
             tggctggcGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAtcttc                                                                                                                                   <  1:2071927/149‑1 (MQ=255)
             tggctggcGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAtcttc                                                                                                                                   <  1:2071925/149‑1 (MQ=255)
             tggctggcGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCAtcttc                                                                                                                                   <  1:2071926/149‑1 (MQ=255)
                     gTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGgt                                                                                                                           >  1:2071999/1‑149 (MQ=255)
                      tCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGgta                                                                                                                          >  1:2071981/1‑149 (MQ=255)
                           ctggGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATc                                                                                                                     >  2:2071978/1‑149 (MQ=255)
                           ctggGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATc                                                                                                                     <  2:2071928/149‑1 (MQ=255)
                             gggTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGc                                                                                                                                <  1:2071930/136‑1 (MQ=255)
                                   ttGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAata                                                                                                             <  2:2071931/149‑1 (MQ=255)
                                   ttGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAata                                                                                                             <  2:2071932/149‑1 (MQ=255)
                                    tGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAatat                                                                                                            >  1:2071972/1‑149 (MQ=255)
                                     gAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATc                                                                                                           >  2:2071982/1‑149 (MQ=255)
                                       aaCCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCt                                                                                                         <  2:2071933/149‑1 (MQ=255)
                                        aCCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTg                                                                                                        >  2:2072002/1‑149 (MQ=255)
                                        aCCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTg                                                                                                        >  2:2071996/1‑149 (MQ=255)
                                         ccGCAGGGGACATCTCGAATCAGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGa                                                                                                       <  2:2071934/149‑1 (MQ=255)
                                           gCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTg                                                                                                        <  1:2071935/146‑1 (MQ=255)
                                            cAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCt                                                                                                    <  2:2071936/149‑1 (MQ=255)
                                            cAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCt                                                                                                    >  1:2071980/1‑149 (MQ=255)
                                               gggACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTa                                                                                                 <  2:2071937/149‑1 (MQ=255)
                                               gggACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTa                                                                                                 <  2:2071938/149‑1 (MQ=255)
                                               gggACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTa                                                                                                 <  1:2071939/149‑1 (MQ=255)
                                                ggACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTa                                                                                                 >  1:2071977/1‑148 (MQ=255)
                                                ggACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGAGCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGAGCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTAc                                                                                                <  2:2071940/149‑1 (MQ=255)
                                                 gACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACg                                                                                               >  2:2071975/1‑149 (MQ=255)
                                                 gACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACg                                                                                               >  1:2072031/1‑149 (MQ=255)
                                                  aCATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACgg                                                                                              <  2:2071941/149‑1 (MQ=255)
                                                  aCATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACgg                                                                                              <  2:2071942/149‑1 (MQ=255)
                                                   cATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGgc                                                                                             >  2:2072015/1‑149 (MQ=255)
                                                       tcGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGt                                                                                         >  2:2071993/1‑149 (MQ=255)
                                                         gCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTACTTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGGCa                                                                                       <  2:2071943/149‑1 (MQ=255)
                                                            tCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGt                                                                                    >  1:2071992/1‑149 (MQ=255)
                                                            tCTGCACGCCCCTGCATAAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGt                                                                                    >  2:2072026/1‑149 (MQ=255)
                                                              tGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTg                                                                                  >  1:2072019/1‑149 (MQ=255)
                                                                gACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAg                                                                                >  2:2071974/1‑149 (MQ=255)
                                                                  cGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCa                                                                              <  1:2071945/149‑1 (MQ=255)
                                                                   gCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCAt                                                                             >  1:2072011/1‑149 (MQ=255)
                                                                       cTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGCCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGc                                                                         >  1:2072037/1‑149 (MQ=255)
                                                                        tGCATTAGATGAGCTGCAGCGTTAACGACGGCTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCa                                                                        <  1:2071948/149‑1 (MQ=255)
                                                                        tGCATTAGATGAGCTGCAGCGGTATCGACGTATGGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCa                                                                        <  2:2071947/149‑1 (MQ=255)
                                                                          cATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCAt                                                                       <  1:2071949/148‑1 (MQ=255)
                                                                          cATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                      >  1:2072009/1‑149 (MQ=255)
                                                                            ttAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCa                                                                    <  2:2071951/149‑1 (MQ=255)
                                                                            ttAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCa                                                                    >  1:2071997/1‑149 (MQ=255)
                                                                              aGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACg                                                                  >  2:2072017/1‑149 (MQ=255)
                                                                                aTGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCAAAGCGTAGAGTAATTGGGCGATCTCACGCTTTGGCATATTCTGCCGGGTAGGATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGtt                                                                >  2:2072003/1‑149 (MQ=255)
                                                                                  gAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTcc                                                              <  1:2071952/149‑1 (MQ=255)
                                                                                       gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGtata                                                         >  1:2071953/1‑149 (MQ=255)
                                                                                       gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGtata                                                         >  1:2072022/1‑149 (MQ=255)
                                                                                        cAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATAc                                                        >  1:2072040/1‑149 (MQ=255)
                                                                                          gCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCg                                                      <  2:2071954/149‑1 (MQ=255)
                                                                                                acgacgGATCGGAATCATCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGa                                                <  2:2071956/149‑1 (MQ=255)
                                                                                                acgacgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGa                                                <  1:2071955/149‑1 (MQ=255)
                                                                                                  gacgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATa                                              >  1:2072004/1‑149 (MQ=255)
                                                                                                  gacgGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATa                                              <  1:2071957/149‑1 (MQ=255)
                                                                                                   acgGATCGGCATCAGCAGGTCACAGCGTAGATTAATTGGGCTAGCTCACGCTTTGGCATCTTCTGCCTGGGAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCAGGCACGTTCCGTATACCGTCAGGATaa                                             <  2:2071958/149‑1 (MQ=255)
                                                                                                     ggATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAAc                                           <  1:2071959/149‑1 (MQ=255)
                                                                                                      gATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACt                                          <  1:2071960/149‑1 (MQ=255)
                                                                                                      gATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACt                                          >  2:2072048/1‑149 (MQ=255)
                                                                                                       aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACt                                          >  1:2071998/1‑148 (MQ=255)
                                                                                                       aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTc                                         >  1:2072030/1‑149 (MQ=255)
                                                                                                        tCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTcc                                        <  1:2071961/149‑1 (MQ=255)
                                                                                                          ggAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTg                                      <  2:2071962/149‑1 (MQ=255)
                                                                                                            aaTCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTTCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCa                                    <  2:2071963/149‑1 (MQ=255)
                                                                                                             aTCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCa                                    <  1:2071964/148‑1 (MQ=255)
                                                                                                                agcagTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                <  2:2071967/149‑1 (MQ=255)
                                                                                                                agcagTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                <  1:2071966/149‑1 (MQ=255)
                                                                                                                 gcagTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCg                               >  2:2072028/1‑149 (MQ=255)
                                                                                                                 gcagTGCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGGTCCGTATACCGTCAGGATAAACTCCTGCACGCCg                               >  2:2072066/1‑149 (MQ=255)
                                                                                                                  cagTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCgg                              <  2:2071968/149‑1 (MQ=255)
                                                                                                                   agTTCACAGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGt                             >  1:2072018/1‑149 (MQ=255)
                                                                                                                       cacaGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTccc                         >  1:2071983/1‑149 (MQ=255)
                                                                                                                         caGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAat                                                                                                              >  1:2072008/1‑62 (MQ=255)
                                                                                                                         caGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCAtt                                                                            >  1:2072007/1‑96 (MQ=255)
                                                                                                                         caGCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAg                       >  1:2072025/1‑149 (MQ=255)
                                                                                                                           gCGTAGATTAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAc                     >  1:2072005/1‑149 (MQ=255)
                                                                                                                                  ttAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAGTATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATa              >  1:2072001/1‑149 (MQ=255)
                                                                                                                                  ttAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATa              >  2:2072041/1‑149 (MQ=255)
                                                                                                                                  ttAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATa              <  1:2071971/149‑1 (MQ=255)
                                                                                                                                  ttAATTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATa              <  1:2071970/149‑1 (MQ=255)
                                                                                                                                    aaTTGGGCGCTCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAgg            >  2:2072012/1‑149 (MQ=255)
                                                                                                                                     aTTGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTACACGCCGGTTCCCAGACCTTCATAGGt           <  2:2071972/149‑1 (MQ=255)
                                                                                                                                      ttGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTa          <  2:2071973/149‑1 (MQ=255)
                                                                                                                                      ttGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTa          >  2:2072039/1‑149 (MQ=255)
                                                                                                                                       tGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaa         <  1:2071976/149‑1 (MQ=255)
                                                                                                                                       tGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaa         <  1:2071974/149‑1 (MQ=255)
                                                                                                                                       tGGGCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaa         <  1:2071975/149‑1 (MQ=255)
                                                                                                                                        gggCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaaa        >  2:2072010/1‑149 (MQ=255)
                                                                                                                                         ggCGATCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAAc       <  2:2071977/149‑1 (MQ=255)
                                                                                                                                             aTCTCACGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAGACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATca   >  2:2072016/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCAGGAAAGCCTGTGGCTGGCGTCTGGCTGGGTCGTTGAAACCGCAGGGGACATCTCGCATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCAC  >  U00096/4634348‑4634638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑