Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 1,152,159 T→G F74C (TTC→TGC)  fabF → 3‑oxoacyl‑[acyl carrier protein] synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,152,1590TG99.2% 500.7 / NA 130F74C (TTC→TGC) fabF3‑oxoacyl‑[acyl carrier protein] synthase 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (66/63);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

AAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTTCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATCTCT  >  U00096/1152015‑1152303
                                                                                                                                                |                                                                                                                                                
aaGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCtgcatt                                                                                                                                              >  2:480106/1‑149 (MQ=255)
 aGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCtgcattc                                                                                                                                             >  1:480093/1‑149 (MQ=255)
   ctctGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGAAAGATGGATGCCTGCATTCaa                                                                                                                                           >  2:480108/1‑149 (MQ=255)
      tGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAatat                                                                                                                                        >  1:480156/1‑149 (MQ=255)
        cTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATg                                                                                                                                       <  1:480067/148‑1 (MQ=255)
          tGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGaa                                                                                                                                    <  2:480068/149‑1 (MQ=255)
           gCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAAt                                                                                                                                   >  1:480116/1‑149 (MQ=255)
               gTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTc                                                                                                                               <  1:480069/149‑1 (MQ=255)
                tCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCg                                                                                                                              <  1:480070/149‑1 (MQ=255)
                 cAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGc                                                                                                                             <  2:480071/149‑1 (MQ=255)
                   gagTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTg                                                                                                                           >  2:480099/1‑149 (MQ=255)
                   gagTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTg                                                                                                                           <  2:480072/149‑1 (MQ=255)
                     gTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGc                                                                                                                         >  1:480141/1‑149 (MQ=255)
                       ggCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGt                                                                                                                       <  1:480074/149‑1 (MQ=255)
                        gCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGtt                                                                                                                      >  1:1283355/1‑149 (MQ=255)
                          aTCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCa                                                                                                                    <  1:480075/149‑1 (MQ=255)
                           tCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAg                                                                                                                   >  1:480130/1‑149 (MQ=255)
                             aGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGc                                                                                                                 >  2:480125/1‑149 (MQ=255)
                              gCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGcc                                                                                                                >  1:480082/1‑149 (MQ=255)
                                     cGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAgg                                                                                                         <  2:480077/149‑1 (MQ=255)
                                      gACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGAGGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGa                                                                                                        <  1:480078/149‑1 (MQ=255)
                                       aCCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGAt                                                                                                       >  1:480134/1‑149 (MQ=255)
                                          aTTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCt                                                                                                    >  1:480176/1‑149 (MQ=255)
                                          aTTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCACTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCt                                                                                                    >  1:480124/1‑149 (MQ=255)
                                              cGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGcc                                                                                                <  2:480081/149‑1 (MQ=255)
                                                 tACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAg                                                                                                                   >  1:480143/1‑127 (MQ=255)
                                                 tACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTg                                                                                             >  2:480118/1‑149 (MQ=255)
                                                  aCTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGa                                                                                            <  2:480082/149‑1 (MQ=255)
                                                    tAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGaaa                                                                                          <  1:480083/149‑1 (MQ=255)
                                                      gcgcCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATa                                                                                        >  2:480136/1‑149 (MQ=255)
                                                      gcgcCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATa                                                                                        >  2:480122/1‑149 (MQ=255)
                                                      gcgcCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATa                                                                                        <  1:480084/149‑1 (MQ=255)
                                                        gcCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAAc                                                                                      >  2:480088/1‑149 (MQ=255)
                                                         ccTATGCAACGAAATTTGCTTGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATaa                                                                                       <  1:480087/147‑1 (MQ=255)
                                                         ccTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAAc                                                                                      <  1:480088/148‑1 (MQ=255)
                                                         ccTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGAAGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACg                                                                                     <  1:480089/149‑1 (MQ=255)
                                                              gCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGa                                                                                   >  2:480180/1‑146 (MQ=255)
                                                               cAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAagag                                                                               <  2:480090/149‑1 (MQ=255)
                                                                aaCGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAgaga                                                                              <  1:480092/149‑1 (MQ=255)
                                                                 aCGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGTCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGaa                                                                             >  1:480154/1‑149 (MQ=255)
                                                                 aCGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGaa                                                                             <  2:480093/149‑1 (MQ=255)
                                                                    aaaTTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGc                                                                          >  1:480168/1‑149 (MQ=255)
                                                                      aTTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCaa                                                                        >  1:480145/1‑149 (MQ=255)
                                                                         tGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAAccc                                                                     <  2:1799407/149‑1 (MQ=255)
                                                                            tGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCa                                                                  <  1:480095/149‑1 (MQ=255)
                                                                             ggCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCAt                                                                 >  2:480165/1‑149 (MQ=255)
                                                                              gCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGACATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCAtt                                                                <  2:480096/149‑1 (MQ=255)
                                                                               cTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTg                                                               >  2:480153/1‑149 (MQ=255)
                                                                                ttAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGACCGCCACCCGCATTgg                                                              >  2:480166/1‑149 (MQ=255)
                                                                                  aGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTg                                                            >  1:480161/1‑149 (MQ=255)
                                                                                   gTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGc                                                           <  2:480098/149‑1 (MQ=255)
                                                                                    tAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGcc                                                          >  1:480173/1‑149 (MQ=255)
                                                                                    tAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGcc                                                          <  1:480099/149‑1 (MQ=255)
                                                                                       aGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCa                                                       >  1:480139/1‑149 (MQ=255)
                                                                                         gATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAAt                                                     >  2:480183/1‑149 (MQ=255)
                                                                                          aTTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAAtt                                                    <  1:480101/149‑1 (MQ=255)
                                                                                             ttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGc                                                 <  1:480102/149‑1 (MQ=255)
                                                                                             ttAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGc                                                 >  1:480146/1‑149 (MQ=255)
                                                                                              tAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCt                                                <  2:480103/149‑1 (MQ=255)
                                                                                              tAACTGTGAGGACATTAACTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGACAACGCAACCCGCATTGGTGCCGCAATTGGCt                                                <  1:480104/149‑1 (MQ=255)
                                                                                               aaCTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTc                                               <  1:480105/149‑1 (MQ=255)
                                                                                                aCTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTcc                                              <  1:480106/149‑1 (MQ=255)
                                                                                                aCTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTcc                                              >  2:480164/1‑149 (MQ=255)
                                                                                                   gtgAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCggg                                           >  1:480129/1‑149 (MQ=255)
                                                                                                        gACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTgg                                      >  1:480188/1‑149 (MQ=255)
                                                                                                           aTTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggcgg                                   >  1:480148/1‑149 (MQ=255)
                                                                                                            ttATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTggcggc                                  >  2:480171/1‑149 (MQ=255)
                                                                                                              aTCTCGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCt                                >  2:480155/1‑149 (MQ=255)
                                                                                                               tctcGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCt                                >  1:480169/1‑148 (MQ=255)
                                                                                                               tctcGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTc                               >  1:480170/1‑149 (MQ=255)
                                                                                                                ctcGCGCAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCg                              <  1:480108/149‑1 (MQ=255)
                                                                                                                   gcgcAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGAc                           >  1:480191/1‑149 (MQ=255)
                                                                                                                    cgcAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGAc                           <  1:480110/148‑1 (MQ=255)
                                                                                                                     gcAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTg                         >  2:480158/1‑149 (MQ=255)
                                                                                                                      cAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGa                        <  1:480111/149‑1 (MQ=255)
                                                                                                                      cAAAGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGa                        <  2:480112/149‑1 (MQ=255)
                                                                                                                       aaaGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGAt                       <  2:480113/149‑1 (MQ=255)
                                                                                                                       aaaGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGAt                       >  1:480159/1‑149 (MQ=255)
                                                                                                                       aaaGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGACGGCCTCGGACTGa                        >  1:515490/1‑148 (MQ=255)
                                                                                                                        aaGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATc                      >  2:480197/1‑149 (MQ=255)
                                                                                                                         aGAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCg                     <  2:480115/149‑1 (MQ=255)
                                                                                                                          gAACAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGAGCga                    <  2:480116/149‑1 (MQ=255)
                                                                                                                           aaCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaa                   <  1:480117/149‑1 (MQ=255)
                                                                                                                            aCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAATCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaag                  <  1:480118/149‑1 (MQ=255)
                                                                                                                            aCAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCg                     >  1:480189/1‑146 (MQ=255)
                                                                                                                             cAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCACCCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCga                    >  2:480202/1‑146 (MQ=255)
                                                                                                                             cAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaaga                 <  2:480119/149‑1 (MQ=255)
                                                                                                                             cAGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaaga                 <  2:480120/149‑1 (MQ=255)
                                                                                                                              aGCGCAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCAATGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaagaa                <  1:480122/149‑1 (MQ=255)
                                                                                                                               gcgcAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGaaa               >  1:480175/1‑149 (MQ=255)
                                                                                                                                 gcAAGATGGATGCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCGGGATTCTGGCCTTGAAATAACGGAAGCGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGTTTGGCGGCCTCGGACTGATCgaagctaac             >  2:480142/1‑144 (MQ=255)
                                                                                                                                    aGATGGATGCCTGCACTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACcac          <  2:480124/149‑1 (MQ=255)
                                                                                                                                     gatggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCgaag                  >  1:480174/1‑140 (MQ=255)
                                                                                                                                     gatggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACcaca         <  1:480125/149‑1 (MQ=255)
                                                                                                                                     gatggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACcac          >  1:480160/1‑148 (MQ=255)
                                                                                                                                      atggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACcacac        <  1:480126/149‑1 (MQ=255)
                                                                                                                                       tggatgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACcacaca       <  2:480127/149‑1 (MQ=255)
                                                                                                                                         gatgCCTGCATTCAATATGGATTTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACAtc     >  1:480150/1‑149 (MQ=255)
                                                                                                                                          atgCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACAtct    >  1:480201/1‑149 (MQ=255)
                                                                                                                                            gCCTGCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATctct  >  2:480209/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGACCATTTCGATACTAGCGCCTATGCAACGAAATTTGCTGGCTTAGTAAAGGATTTTAACTGTGAGGACATTATCTCGCGCAAAGAACAGCGCAAGATGGATGCCTTCATTCAATATGGAATTGTCGCTGGCGTTCAGGCCATGCAGGATTCTGGCCTTGAAATAACGGAAGAGAACGCAACCCGCATTGGTGCCGCAATTGGCTCCGGGATTGGCGGCCTCGGACTGATCGAAGAAAACCACACATCTCT  >  U00096/1152015‑1152303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑