Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096423,5520TG20.0% 362.2 / 23.3 152G345G (GGT→GGG) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (92/28);  new base G (1/29);  total (94/58)
Fisher's exact test for biased strand distribution p-value = 4.39e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 161 total aligned reads displayed.

CCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTG  >  U00096/423404‑423700
                                                                                                                                                      |                                                                                                                                                    
ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGt                                                                                                                                                      <  1:409514/149‑1 (MQ=255)
ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGt                                                                                                                                                      >  2:409678/1‑149 (MQ=255)
ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGt                                                                                                                                                      >  1:409538/1‑149 (MQ=255)
  tgCTTGCCTAAGCTGGATTATCTGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGTAATATGG‑ACGGCGGGCGGCTGGATGTGGGCCATATGC‑GGGGGGAGGCGGGTgg                                                                                                                                                    <  2:409515/148‑1 (MQ=255)
       gCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTTATTGAATTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTTAATGCGCCGTGGAATTTGG‑AGGGCTGGCGGCTGGATGTGTTGCATATGC‑GGGGGGAGGCGGGTGGGgcgc                                                                                                                                               <  2:409517/149‑1 (MQ=255)
                ggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                                                                      >  2:409656/1‑149 (MQ=255)
                ggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                                                                      >  2:409699/1‑149 (MQ=255)
                 gATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAatat                                                                                                                                     >  2:409700/1‑149 (MQ=255)
                 gATTATCAGTCGGAAAGGCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑CCGGCTGGCGGCTGGATGGGGGGCATATGC‑TGGGGGCGGCGGGGGGGGCGCGCAATAatat                                                                                                                                     <  1:409521/149‑1 (MQ=255)
                    tATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATgca                                                                                                                                  >  2:409661/1‑149 (MQ=255)
                     aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATgcag                                                                                                                                 >  1:409652/1‑149 (MQ=255)
                      tCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagc                                                                                                                                >  2:409588/1‑149 (MQ=255)
                      tCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagc                                                                                                                                >  2:409629/1‑149 (MQ=255)
                        agccGGAAAGGCGGGGGAATGAAATTAATCGCGGGCAAGCCAGTATTGCCCGCCACGGGCTCAAAGCGCCGTGTAATATGC‑ACGGCTGGCGGCGGTATGGGGGGAATAGGC‑GGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCAc                                                                                                                              <  2:409522/146‑1 (MQ=255)
                         gTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGAGGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAAGAATATGCAGCACg                                                                                                                             >  2:409549/1‑149 (MQ=255)
                          tCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGt                                                                                                                            >  1:409644/1‑149 (MQ=255)
                              aatgTCTGGTGAATGAATTTTAGCGCGGGGAAGACAGTATTGTCCGCCACTGCCTGAACGCGCCGTGGAATATGG‑ACGGCGGGCGGCTGGAGGTGGTGCATATGC‑GGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGcc                                                                                                                        <  2:409524/146‑1 (MQ=255)
                               aaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                                                                       >  1:409684/1‑149 (MQ=255)
                                 ggcTGGTGAATTAAATTTAGCGGGGGTACGACAGTATTGTCCGCCACGGGCTGAACGCGCGGGGTATTTTGG‑CCGGCGGGGGGCGGGTGGGGGTGAATATGC‑GGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCggg                                                                                                                     <  2:409594/147‑1 (MQ=255)
                                       tgaatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATTG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCAcc                                                                                                               >  2:409640/1‑149 (MQ=255)
                                           tgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                                                                           >  1:409601/1‑149 (MQ=255)
                                            gaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATc                                                                                                                  >  1:409731/1‑141 (MQ=255)
                                            gaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgc                                                                                                          >  2:409689/1‑149 (MQ=255)
                                             aaaTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                                                                         >  1:409659/1‑149 (MQ=255)
                                               aTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgc                                                                                                          >  1:409566/1‑146 (MQ=255)
                                                tttATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggcg                                                                                                      >  1:409575/1‑149 (MQ=255)
                                                  tATCGCGGGTACGACAGTATTGCCCCCCCCTGGCTGAAAGCGCCGTGGAATAGGG‑ACGGCGGGCGGCGGGCGGTGGGGCATAGGC‑GGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaa                                                                                                    <  1:1229431/149‑1 (MQ=255)
                                                  tATCGCGGGTAAGACAGTATTGCCCGCCACTGGCTGAACGCGCCGTGTAATATGG‑ACGGCTGGCGGCGGTATGTGGTGCATATGC‑GGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaa                                                                                                    <  2:409527/149‑1 (MQ=255)
                                                  tATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAACGCGCCGTGGAATAGGG‑CCGGCTGGCGGCTGGATGTGGTGCATATGC‑GGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaa                                                                                                    <  2:409528/149‑1 (MQ=255)
                                                  tATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaa                                                                                                    >  1:409529/1‑149 (MQ=255)
                                                   aTCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaa                                                                                                   >  1:409702/1‑149 (MQ=255)
                                                     cgcgGGGACAACTGTTTTGTCCGCCCCTGGCTGATCGCGCCGGGGAATAGGT‑CCGGCGGGCGGCGGTTGGGGGGGCATAGGC‑GGGGGCCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaaga                                                                                                 <  2:409572/149‑1 (MQ=255)
                                                         gggAAGAAAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑CCGGCTGGCGGCTGGAGGTGGTGCATATGG‑TGGGGGAGGCGGGGGGGGCGCGCAATAATATGCTGCACGTTGCGGGGATACCCGATGCGGCGAATGAAAcc                                                                                             >  2:409683/1‑149 (MQ=255)
                                                           tatgACTGTTTTGGCCGCCAGTGGCTGAATGCGCCGGGTAATAGGG‑CGGGGGGGGGGGTGGTTGGGGTGAATTTGG‑GGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCa                                                                                           <  2:409567/146‑1 (MQ=255)
                                                           gAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCa                                                                                           >  2:409672/1‑149 (MQ=255)
                                                             tgACAGAATTGTCCGCCATTGTCTTAAAGGGCCTTGTTTTTTGT‑ACGGTGGGCGGTGGGTTGTGGTTAATATGT‑TGGGGTTGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCTGGATCACAGTAGCGGCGAAAGAAACCCAGc                                                                                         <  2:409584/148‑1 (MQ=255)
                                                             aGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                                                         >  2:409725/1‑149 (MQ=255)
                                                             aGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                                                         >  1:409714/1‑149 (MQ=255)
                                                             aGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                                                         >  1:409648/1‑149 (MQ=255)
                                                              gACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGGAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGc                                                                                         >  1:409718/1‑148 (MQ=255)
                                                              gACAGTATTGCCCGCCACTGGCTGAAAGCGCCGTGTAATATGG‑CCGGCTGGCGGCTGGAGGTGGTGCATATGC‑GGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGcc                                                                                        <  2:409531/149‑1 (MQ=255)
                                                                cAGTATTGTCCGTCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaaga                                                                                                 >  1:409687/1‑138 (MQ=255)
                                                                cAGTATTGTCCGTCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCgg                                                                                      >  1:409688/1‑149 (MQ=255)
                                                                cAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCgg                                                                                      >  1:409647/1‑149 (MQ=255)
                                                                 aGTATTGTCCGCCACTGGCTCAACGCGCCGGGTAATATGG‑ACGGCGGGCGGCGGGCTGGGGTGCATATGC‑GGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                                     <  2:409533/149‑1 (MQ=255)
                                                                   tATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAg                                                                                   >  1:409653/1‑149 (MQ=255)
                                                                    aTTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGT‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                                                  <  2:409535/149‑1 (MQ=255)
                                                                       gTCCGCCACTGGCTGAACGCGCCGGGGAATATGC‑CCGGCGGGCGGAGGGATGGGGTGCATATGC‑GGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGta                                                                               <  1:711947/149‑1 (MQ=255)
                                                                         cccccACTGGTGTAAGGCGCCGTGG‑ATATGGCCCGCCTGGGGGTGGGAGGTTGTTATTATGC‑GGGGGGTGGGGGGGGGCGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtata                                                                             <  1:409602/146‑1 (MQ=255)
                                                                           gcaaCTGGGTTAAAGGGCCGTGTAATTTGG‑ACGGCTGGCGGTTGGTTGTGGGGATTATGC‑GGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATAtt                                                                           <  2:409537/146‑1 (MQ=255)
                                                                           gCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATAtt                                                                           <  2:409538/149‑1 (MQ=255)
                                                                             cACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGt                                                                         >  2:409667/1‑149 (MQ=255)
                                                                              aCTGGCTGAACGCGCGGTGTAATATGG‑CCGGCGGGCGGCTGGATGTGGGGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                                                                        <  2:409541/149‑1 (MQ=255)
                                                                              aCTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCCCGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCg                                                                                 >  1:409707/1‑140 (MQ=255)
                                                                              aCTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGT‑GGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCg                                                                       >  1:409562/1‑149 (MQ=255)
                                                                               cTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                                                                            >  1:409736/1‑144 (MQ=255)
                                                                                      aacgcgcCGTGTAATATGG‑AGGGCGGGCGGGTGTATGTGGGGATTATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa                                                                <  2:409544/146‑1 (MQ=255)
                                                                                      aaaGCGCCGGTTAATATGG‑ACGGCTGGCGGCTGTTTTTGGTGCATATGC‑GGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa                                                                <  1:409543/149‑1 (MQ=255)
                                                                                       aaGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAt                                                               >  2:409669/1‑149 (MQ=255)
                                                                                        aGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtt                                                              >  1:409671/1‑149 (MQ=255)
                                                                                         gcgcGGTGTAATATGG‑CCGGCGGGCGGCTGGATGGGGTGCATAGGG‑GGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                             <  2:409545/149‑1 (MQ=255)
                                                                                            ccGTGTAATTTGG‑ACGGCGGGGGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTgg                                                          <  2:409547/149‑1 (MQ=255)
                                                                                            ccGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTgg                                                          >  1:409677/1‑149 (MQ=255)
                                                                                             cGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                                         >  1:409582/1‑149 (MQ=255)
                                                                                              gTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCg                                                        >  2:409680/1‑149 (MQ=255)
                                                                                                ggAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑GGGGGGCGGCGGGTGGGGCGCGCAATAAAATGCAGAACGTTGCCGGGATCACAGAAGCTGCTAAAGAAAACCAGCCGGAAGAGGTTAATGTCAGCGAACATTTTGGCGAt                                                      >  2:3440855/1‑149 (MQ=255)
                                                                                                 gAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATAAGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATg                                                     >  2:409766/1‑149 (MQ=255)
                                                                                                     tatgcccggcgggcggctgtTTGTGTTGCATAGGC‑GGGGGTCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCAc                                                  <  2:409571/143‑1 (MQ=255)
                                                                                                     atGG‑ACGTCTGGCTGCTGGATGGGGTGAATATGC‑TGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                                                 <  1:409549/149‑1 (MQ=255)
                                                                                                     atGG‑ACGGCTGGCGGCTGGATGCGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                                                 >  1:409679/1‑149 (MQ=255)
                                                                                                          ccggctggcggcgggATGTGGTGCATAGGC‑TGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCaa                                             <  2:409550/148‑1 (MQ=255)
                                                                                                             gcgggcggctggATGTGGTGCATATGC‑TGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATgg                                          <  1:409551/146‑1 (MQ=255)
                                                                                                               tggcggctggATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGtt                                        >  1:409666/1‑149 (MQ=255)
                                                                                                               tggcggctggATGAGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCAc                                                                                                                >  1:409555/1‑77 (MQ=255)
                                                                                                                 gcggctggATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTAc                                      >  1:409698/1‑149 (MQ=255)
                                                                                                                  cggctggATGTGGTGCAT‑TGCGGGGGGGTGGCGGGGGGCGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACa                                     <  2:409554/149‑1 (MQ=255)
                                                                                                                    gctggTGGTTGTGCATATGC‑TGGGGGCGGCGGGGGGGGCGCGCAAGAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAgg                                   <  1:409557/149‑1 (MQ=255)
                                                                                                                    gctggATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGaa                                                                                    <  2:409556/100‑1 (MQ=255)
                                                                                                                      tggATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGcc                                 >  2:409712/1‑149 (MQ=255)
                                                                                                                         aTGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAt                              >  1:409696/1‑149 (MQ=255)
                                                                                                                          tggggttCATAGGC‑GGGGGCCGGCGGGCGGCGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtg                             <  2:409610/146‑1 (MQ=255)
                                                                                                                             ggtgCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg                          >  2:409692/1‑149 (MQ=255)
                                                                                                                                   tatGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATg                    <  1:409558/149‑1 (MQ=255)
                                                                                                                                    atGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgc                   >  2:409759/1‑149 (MQ=255)
                                                                                                                                      gggtGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgcc                  <  2:409583/146‑1 (MQ=255)
                                                                                                                                      gC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                                         <  1:409561/109‑1 (MQ=255)
                                                                                                                                      gC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccg                 >  1:409729/1‑149 (MQ=255)
                                                                                                                                         tGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccgcc               >  2:409705/1‑149 (MQ=255)
                                                                                                                                         tGGGGGAGGCGGGT‑GGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCa              <  2:409562/149‑1 (MQ=255)
                                                                                                                                           ggggAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCAt             <  2:409569/149‑1 (MQ=255)
                                                                                                                                             ggAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGACGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGa           >  2:409778/1‑149 (MQ=255)
                                                                                                                                              gcggCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCCGCCGGACGCGTATATTGTCGGCGAACATTTTGGCGCTGCACGGCAATGGTTACAGGCCGATGGGGACGATGCCGCCCTGaa          >  1:409760/3‑149 (MQ=255)
                                                                                                                                                ggCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                                                                           <  1:409574/50‑1 (MQ=255)
                                                                                                                                                ggCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACt        >  1:409709/1‑149 (MQ=255)
                                                                                                                                                ggCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCTCCGAAGCGGCGAAAGAAACCCACCCCGCAGCGTATATTGACGGAGAACATTTTGGCGATGCACGTCAATGTTTACAGGCCGATGTGGAAGATGCCGCCATGAACt        <  2:1932343/149‑1 (MQ=255)
                                                                                                                                                ggCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgc                                                                                                          <  1:1843582/51‑1 (MQ=255)
                                                                                                                                                 gCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTa       <  2:409575/149‑1 (MQ=255)
                                                                                                                                                   ggggggggCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                                     <  1:409578/69‑1 (MQ=255)
                                                                                                                                                   ggggggggCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                                                                        <  1:409579/82‑1 (MQ=255)
                                                                                                                                                      tGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTg  <  2:409601/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGG‑ACGGCTGGCGGCTGGATGTGGTGCATATGC‑TGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTG  >  U00096/423404‑423700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑