Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U000961,708,7510AG32.7% 42.5 / 39.1 55E611E (GAA→GAG) rsxCSoxR [2Fe‑2S] reducing system protein RsxC
Reads supporting (aligned to +/- strand):  ref base A (12/23);  new base G (11/6);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 7.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGC  >  U00096/1708610‑1708879
                                                                                                                                             |                                                                                                                                
ccGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATCCGGAACCAGCACCACAGGTCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaag                                                                                                                           <  2:1633168/149‑1 (MQ=255)
        aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                   <  1:1633170/149‑1 (MQ=255)
            gctgaaGCTGGAACAGCAACAGGATAATCAGGAAGCAGAAAAAGAGGCCGATCCTCGTAAAGCAGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATc                                                                                                               <  2:1633176/145‑1 (MQ=14)
             cgcAAGCTGGAACAGCAACAGCCTACTCCGGACCCAGCACCCGAGGCCGATCCCCGCAAACCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATcc                                                                                                              <  2:1633172/149‑1 (MQ=14)
               cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgc                                                                                                            <  2:1633174/149‑1 (MQ=255)
                 aGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCgcgc                                                                                                          <  2:1633177/149‑1 (MQ=255)
                                          ggAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                   <  1:1633181/115‑1 (MQ=255)
                                          ggAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                   >  2:1633181/1‑115 (MQ=255)
                                                       caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGCTCGATCCGCGCAAAGCCGCCGTCGAAGAAGCTATTGCACGTGCCAAAGc                                                                    >  2:1633274/1‑149 (MQ=11)
                                                         ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGAGCTCACAGAAGAACAGGTAGCTCCGCGCAAAGCAGCAGTAGAAGCCGCTATTGCCCGCGCGCAAGctc                                                                  >  2:1633476/1‑147 (MQ=11)
                                                             gATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAc                                                                                                                        <  1:1633188/91‑1 (MQ=255)
                                                             gATCCTCGCAAAGCAGCCGTCGAAGCAGCTATAGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                     <  1:1633189/94‑1 (MQ=11)
                                                             gATCCCCGCAAACCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCaga                                                                                                                             <  1:1633190/86‑1 (MQ=11)
                                                                 cgcgCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg                                                          >  2:1633251/1‑149 (MQ=255)
                                                                    gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                    >  1:1633284/1‑88 (MQ=255)
                                                                      aaaccggccgTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa                                                     <  2:1633193/143‑1 (MQ=255)
                                                                            gccgTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                   <  1:1633196/81‑1 (MQ=255)
                                                                            gccgTCGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGa                                                                                                                 <  1:1633199/83‑1 (MQ=255)
                                                                             ccttCGAAGCCGCTATCGCCCGAGCCAAAGCGCGAAAGCTGGAACTTCTACATTCTTATGCGGAACCAGAACACCTGGCCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAg                                              <  2:1633203/146‑1 (MQ=11)
                                                                             ccgTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                    >  1:1633297/1‑79 (MQ=255)
                                                                                       cGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCgcgc                                                                                                          >  1:1633307/1‑79 (MQ=255)
                                                                                        gatataGCCCGTGCCAAAGCTCGAATGCTTGACGAGCATCAGGATAATTAGGAACCAGCACACGAGGCCGATCCTCGCAAAGCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                   <  2:1633218/147‑1 (MQ=2)
                                                                                        gCTATCGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCg                                                                                                                  <  1:1633206/70‑1 (MQ=25)
                                                                                            ttGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGcc                                                                                        >  1:1633254/1‑92 (MQ=255)
                                                                                               cccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                    >  1:1633208/1‑61 (MQ=255)
                                                                                                ccGTGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAgg                                                                                                                     <  2:1633313/59‑1 (MQ=11)
                                                                                                ccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAgg                                                                                                                     >  1:1633313/1‑59 (MQ=255)
                                                                                                ccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGtt                                                                                                                   <  2:1633208/61‑2 (MQ=255)
                                                                                                    gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAgaagaa                                                                                                                         <  1:1633316/51‑1 (MQ=255)
                                                                                                    gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAgaagaa                                                                                                                         >  2:1633316/1‑51 (MQ=255)
                                                                                                      cAAAGCGCTCAAGATTGAACAGCAACTGGCTAATGCGCAACGACAAGAAGTGTGCGTTCCGCTGACTGGCGCCGTCGACCCCTCTATTGCCCGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                     <  1:1633256/149‑1 (MQ=9)
                                                                                                      cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAgaagaa                                                                                                                         >  1:1633319/1‑49 (MQ=255)
                                                                                                      cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaaa                                                                                                       <  2:1633318/67‑1 (MQ=255)
                                                                                                      cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCaaa                                                                                                       >  1:1633318/1‑67 (MQ=25)
                                                                                                       aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGt                                                                                                                    >  1:1633321/1‑53 (MQ=255)
                                                                                                       aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCgcgc                                                                                                          <  2:1633320/63‑1 (MQ=255)
                                                                                                       aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCgcgc                                                                                                          >  1:1633320/1‑63 (MQ=255)
                                                                                                            gcgcAAGCTGTAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCATCGCAAAGCCTCCGTCGAAGCCTCTATTGCCATTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATc               <  2:1633210/149‑1 (MQ=14)
                                                                                                            gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                                                   <  1:1633209/49‑1 (MQ=38)
                                                                                                               cAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACa                                                                                                                       <  2:1633328/42‑1 (MQ=255)
                                                                                                               cAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACa                                                                                                                       >  1:1633328/1‑42 (MQ=255)
                                                                                                                    tGGAACGGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                           >  1:1633211/1‑97 (MQ=37)
                                                                                                                    tGGAACGGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                                                           <  2:1633211/97‑1 (MQ=37)
                                                                                                                    tGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCTGCCGTCGa                                                                                            >  1:1633327/1‑64 (MQ=25)
                                                                                                                       aaCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGt                                                                                               >  1:1633438/1‑58 (MQ=11)
                                                                                                                         cAGCAACAGTCTATTTCTTAACGAGAAGTAGAGGTCGATCCGCTAAAATCTTCCGTCTAAGCCGCTAATGCCCTTGACAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTCGATCCGCGCAAAgccgc  <  2:1633243/149‑1 (MQ=11)
                                                                                                                         cAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccgc                                                                                                  <  2:1633213/53‑1 (MQ=35)
                                                                                                                            cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcgttatc         >  1:3721470/1‑133 (MQ=255)
                                                                                                                                 ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg                                                                                       >  1:1901776/1‑56 (MQ=35)
                                                                                                                                 ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt                                           >  1:1633302/1‑100 (MQ=37)
                                                                                                                                    tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAt                                                                                   <  1:2725909/57‑1 (MQ=35)
                                                                                                                                    tAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTaa                                         >  2:1633334/1‑99 (MQ=37)
                                                                                                                                    tAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCg                                                                                             >  2:1633336/1‑47 (MQ=255)
                                                                                                                                      aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                      >  1:1633333/1‑100 (MQ=255)
                                                                                                                                          ggAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTAGCAGCGGCTATTGCCCg                                                                             >  1:1633355/1‑57 (MQ=11)
                                                                                                                                            aaCCAGAAGAACAGGTCGATCCGCGCAAAGccgccg                                                                                                >  1:1633339/1‑36 (MQ=40)
                                                                                                                                             |                                                                                                                                
CCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGC  >  U00096/1708610‑1708879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑