New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	696863 =	73 (0.330)	46 (0.220) +GAAGAAACAATC	22/268	17.8	26.4%	intergenic (‑33/+2)	glnW/glnU	tRNA‑Gln/tRNA‑Gln
	?	U00096	= 697134	207 (0.930)					intergenic (‑1/+379)	metT/asnB	tRNA‑Met/asparagine synthetase B
Rejected: Coverage evenness skew score above cutoff.

CGCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  U00096/697000‑696863 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGCCACC  <  U00096/697134‑697058                                                                                                                                           ||||||||||||                                                                              CGCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAAT                                                                                >  2:519290/1‑149  GCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATC                                                                               >  2:519284/1‑149      GGTGTGCGAGTTCAAGTCTCCCCTCCCTCACACTTCACCAGAAAGCGCTGTACGTTTGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGC                                                                           <  2:519268/149‑1            CGAGGTCAAGTCGCGCCGCCCGCACCCTTCACCCGACAGCGTTGTCCTCATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTA                                                                     <  2:519304/149‑1              AGTTCAAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAG                                                                    <  1:519290/148‑1                 tcacgcCTCGCCTCCCTCACCCTTCCCGCGAAAGCGCTGTACGTATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCA                                                                <  2:519320/143‑1                   AAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCTAAGAAACAAACTCGCTACGAAGCTCAGT                                                              >  2:519253/1‑149                   AAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAGCAATCTCGCTACGAAGCTCAGT                                                              >  1:519289/1‑149                       CTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTCGCTACGTAGCGCAGTTAGT                                                          >  2:519267/1‑149                       CTCGACTCCCTCACCCTTCTCCTGTAAGCGTTGGTCGTATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGT                                                          <  2:519293/149‑1                        TCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGACACAAACTCGCTACGAAGCACAGTTAGTT                                                         >  2:519280/1‑149                        TCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTT                                                         >  2:519281/1‑149                          gaCTCCCGAACAATTCACCAGAAAGCGCTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAG                                                       <  2:519266/147‑1                          GCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCTTATAATCAATCTCGCTTCGAAGCTCAGTTGGTTAG                                                       >  2:519245/1‑149                           CCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCTAAGAAACAATCTCGCTACGAAGCTCAGTTTGTT                                                         >  2:519252/1‑146                            CTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGT                                                                      >  1:519260/1‑132                            CTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGT                                                                      <  2:519260/132‑1                             TCCCGCACCATTCTCCAGAAAGCGTTGTTCGTATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGC                                                    <  2:519276/149‑1                                 GCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCTAATAAGAAAACTCGCTTCGTAGCTCAGTTGTTTAGATCACga                                                >  2:519250/1‑147                                  CACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAACCAAACTGGCTACCTAGCGCAGTTGGTTAGAGAACAac                                               >  1:519277/1‑147                                     CATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGGAGCAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACGTCAat                                            >  2:519183/1‑147                                              gtcAGCGTTGTACGTATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGAT                                   <  2:519289/146‑1                                               AAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATG                                  <  2:519296/149‑1                                                AAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCG                                                                                          >  1:519251/1‑92                                                 AGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGG                                <  2:519283/149‑1                                                    GTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCTAATACAAAATCTGGATACGAAGCACAGTTAGTTAGAGCACATCACTCATAAAGAataggtc                             >  2:519249/1‑142                                                       gttcttATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACA                          <  2:519277/143‑1                                                             ATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAG                                                       <  1:519281/114‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGGAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTC                                           <  1:519183/125‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATG                                  <  1:519267/134‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTC                                           <  1:519242/125‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCAC                                                  <  1:519343/118‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTAC                                                                        <  1:519279/96‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTCGCTACGAAGCACAGTTGGTTAGAGCACAACACTCATAATGAAGCCGTCACAGGATCGA                   >  2:519178/1‑149                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAAT                                                                                <  1:519311/88‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAA                                                                                        <  1:519361/80‑1                                                              TGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAA                                                                                        <  1:519259/80‑1                                                               GGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAA                  <  1:519315/149‑1                                                               GGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGA                                                                                         <  1:519332/78‑1                                                                GGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTT                      >  2:519179/1‑144                                                                GGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTT                      <  1:519179/144‑1                                                                   TATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCC              <  1:519284/149‑1                                                                   TATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCC              >  1:519173/1‑149                                                                       GCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACG                                                                       <  1:1512136/88‑1                                                                       GCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAG                                                                                       >  1:519200/1‑72                                                                          AAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAG       >  1:519172/1‑149                                                                          AAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAG       <  1:519178/149‑1                                                                           AGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGC      <  2:519278/149‑1                                                                           AGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGC      <  1:519180/149‑1                                                                           AGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGCTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGC      >  1:519171/1‑149                                                                             CGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGCCA    <  1:519252/149‑1                                                                               GTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGCCACC  >  2:519170/1‑149                                                                                       CCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGA                   <  1:519177/124‑1                                                                                             TTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATCGAAGAAACAATCTGGCTACGTAGCTC                                                                 <  2:519291/72‑1                                                                                                                                            AAGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAA                  <  2:519200/72‑1                                                                                                                                             AGAAACAATCTGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACA                          <  2:519297/63‑1                                                                                                                                           ||||||||||||                                                                              CGCAAGGTGTGCGAGTTCAAGTCTCGCCTCCCGCACCATTCACCAGAAAGCGTTGTACGGATGGGGTATCGCCAAGCGGTAAGGCACCGGTTTTTGATACCGGCATTCCCTGGTTCGAATCCAGGTACCCCAGCCATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  U00096/697000‑696863 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGCTACGTAGCTCAGTTGGTTAGAGCACATCACTCATAATGATGGGGTCACAGGTTCGAATCCCGTCGTAGCCACC  <  U00096/697134‑697058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.