Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG24.7% 329.0 / 25.1 151intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (18/95);  new base G (35/2);  total (53/98)
Fisher's exact test for biased strand distribution p-value = 1.63e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 153 total aligned reads displayed.

TTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACT  >  U00096/422282‑422576
                                                                                                                                                   |                                                                                                                                                   
        gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                <  1:312875/145‑1 (MQ=255)
        gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccc                                                                                                                                            >  2:312936/1‑149 (MQ=255)
          ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCGCCTccccc                                                                                                                                          >  2:312996/1‑149 (MQ=255)
          ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                          <  1:312876/149‑1 (MQ=255)
          ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                          >  2:312940/1‑149 (MQ=255)
            gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCATCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                        <  2:312877/149‑1 (MQ=255)
            gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                        <  2:312878/149‑1 (MQ=255)
                  cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATcc                                                                                                                                  <  2:312881/149‑1 (MQ=255)
                   tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATccg                                                                                                                                 >  2:313022/1‑148 (MQ=255)
                    gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGGCGCCCCCAAAGATCCtt                                                                                                                                >  2:312942/1‑149 (MQ=255)
                    gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                <  1:312882/149‑1 (MQ=255)
                      tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCGAAATATCCTTCa                                                                                                                              >  1:313040/1‑149 (MQ=255)
                       gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGCAAGTTCTCCGAGCCCGTCCGCCGCCCCCCAAGATCCTGCAg                                                                                                                             >  2:312988/1‑149 (MQ=255)
                       gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                             <  1:312883/149‑1 (MQ=255)
                       gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAGTCCGTCAg                                                                                                                             >  2:312947/1‑149 (MQ=255)
                            gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                        <  2:312885/149‑1 (MQ=255)
                             acttaTGTTTAAACGCCGCCACGTTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTGAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                       <  2:1116153/145‑1 (MQ=255)
                                gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                     <  1:312889/148‑1 (MQ=255)
                                gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATAGCCGTCAGATGATGAGt                                                                                                                    >  2:312975/1‑149 (MQ=255)
                                gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                       <  1:312888/146‑1 (MQ=255)
                                 aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                   <  2:312891/149‑1 (MQ=255)
                                 aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCGCCCACAGATCCTGCAGATGATGAGTg                                                                                                                   >  2:312939/1‑149 (MQ=255)
                                   gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGGGCCGCCCCCACAGATCCGTCAGATGAGGAGTggt                                                                                                                 >  2:313043/1‑147 (MQ=255)
                                   gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGGGCGCCTCCCCCACATATCGTGAAGATGGTGAGTGAt                                                                                                                 >  2:312982/1‑149 (MQ=255)
                                   gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                 <  1:312892/149‑1 (MQ=255)
                                    tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCAGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATc                                                                                                                <  2:312893/149‑1 (MQ=255)
                                      tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                              <  1:312895/149‑1 (MQ=255)
                                         aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                           <  2:312896/149‑1 (MQ=255)
                                            ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                        <  1:312898/149‑1 (MQ=255)
                                              gccACGATCGGCAGCCGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                      <  1:312899/149‑1 (MQ=255)
                                               ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTGCAGATGTTGAGTGATCCTGCATTAggg                                                                                                     >  2:313031/1‑148 (MQ=255)
                                                 aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCGCCCCCAAATATCCGTCAGATGAGGAGTGATCCTGCATGAGGCTa                                                                                                   >  2:312960/1‑149 (MQ=255)
                                                 aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                   <  2:312901/149‑1 (MQ=255)
                                                   gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                 <  1:312902/149‑1 (MQ=255)
                                                     tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                               <  1:312903/149‑1 (MQ=255)
                                                      cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                              <  2:312906/149‑1 (MQ=255)
                                                      cGTCAGCTGGCGGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTCTCCGCCCCCGCCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                              <  2:312907/149‑1 (MQ=255)
                                                          aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                          >  2:312970/1‑149 (MQ=255)
                                                             tggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAt                                                                                       <  2:312910/149‑1 (MQ=255)
                                                               gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATc                                                                                      <  1:312912/148‑1 (MQ=255)
                                                               gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                     <  2:312913/149‑1 (MQ=255)
                                                                ctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAg                                                                                    <  2:312914/149‑1 (MQ=255)
                                                                  gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCAATATCCTTCAGATGATGAGGGGGCCGGCTTTAGGGGATGGGAAGGGGATc                                                                                      >  2:313033/1‑145 (MQ=255)
                                                                   aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGa                                                                                        <  1:312915/142‑1 (MQ=255)
                                                                     aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGTCCGCCGCCCCCCAATATCCTTCAGATGATGAGTGGGCCGGCATTAGGCGAGGGCGAGGGGATCAGAtgtt                                                                               >  2:313048/1‑149 (MQ=255)
                                                                     aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATGCTTGAGAGGATGAGTGATCGTGGGTGAGGGTAGGGCAAGGGGGTCCGGtttt                                                                               >  1:313068/1‑149 (MQ=255)
                                                                      aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                              <  1:312919/149‑1 (MQ=255)
                                                                      aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                              <  2:312918/149‑1 (MQ=255)
                                                                       ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                             <  1:312920/149‑1 (MQ=255)
                                                                          gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                          <  2:312922/149‑1 (MQ=255)
                                                                          gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCGTCAGATGATGAGTGATCCTGGAGTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                          >  2:312998/1‑149 (MQ=255)
                                                                           tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCATGGTGATCAGATTTTCATcac                                                                         <  1:312923/149‑1 (MQ=255)
                                                                             aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                       <  1:312925/149‑1 (MQ=255)
                                                                              tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCCCAAATATCCGTCAGATGATGAGTGATGCTGCGGTAGGGGATGGGAAGGTGATCAGAGTTTGATCACAgg                                                                      >  2:313063/1‑149 (MQ=255)
                                                                                cTTGCTTTCTGTAAGGCGTTGCAGGAGGTAATCCTGATCCGGCTATTCCGAAAGTTATGCGCCCGCGTCCTCGTCCCCCATTTCTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTTATCAGATTTTCTTCACTgggg                                                                    <  1:312926/149‑1 (MQ=255)
                                                                                     gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                               <  2:312930/149‑1 (MQ=255)
                                                                                     gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                               <  2:312929/149‑1 (MQ=255)
                                                                                       tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGATATCCGGGGCCGGGCGGCGCCCCCCAAGATACGGCAGAGGATGAGTGATGAGGCAGTAGGGTATGGCAAGGTGATCAGATATTAATCACAGGGGTAGTatg                                                             >  2:313017/1‑149 (MQ=255)
                                                                                        cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTCTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                 <  1:312932/112‑1 (MQ=255)
                                                                                        cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                            <  1:312934/149‑1 (MQ=255)
                                                                                           tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                          <  1:312936/148‑1 (MQ=255)
                                                                                               ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                     <  1:312938/149‑1 (MQ=255)
                                                                                               ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                     <  2:312937/149‑1 (MQ=255)
                                                                                                cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGGTCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                    <  1:312940/149‑1 (MQ=255)
                                                                                                  gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCAGCCCCCAAATATAAAGAAGATAATTAGTGGTGATGGGTTTGGATATGGCAAGGTGAGAAGAATTTATTGACAGGGGTTTTATGATGTTTAATga                                                  >  2:312992/1‑148 (MQ=255)
                                                                                                  gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCCAATAACCTTCAGATGATGAGTGATCCTGCAGTAGGGTTTGGCAAGGTGTTCAGGTTTTAATGAGAGGGGAATTATGATGTTTAATga                                                  >  2:313075/1‑148 (MQ=255)
                                                                                                   ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                 <  1:312942/149‑1 (MQ=255)
                                                                                                   ttCAGGAGGAAATCCTGATCCGGATATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATTTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                 <  2:312943/149‑1 (MQ=255)
                                                                                                      aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGGCCGCCCCCAAAAATCCTTCAGAGGATGAGTGAGCCGGCATTAGGCTATGGCAAGGTGAGCAGATTTTAATCCCAGGGGAATTATTTTGTTTAATGCATgg                                              >  2:313003/1‑149 (MQ=255)
                                                                                                      aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCACGGTGATCAGATTTTCATCAAAGGGGAATTATGATGTTAAATGAATgg                                              >  2:313034/1‑149 (MQ=255)
                                                                                                      aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATAACCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGGAAGGTGATCAGAGTTTCATCACAGGGGAATTATGATGTTAAATGAATgg                                              >  2:313027/1‑149 (MQ=255)
                                                                                                           gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                         <  1:312947/149‑1 (MQ=255)
                                                                                                           gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                         <  2:312945/149‑1 (MQ=255)
                                                                                                           gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                         <  2:1007999/149‑1 (MQ=255)
                                                                                                                 cTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGTCCGCGGCCCCCAAAAATCCTGCAGATGTGGAGTGGTGAGGGGTTAGGGTTGGGGCTGGGGATCAGATTTTGATCAAATGGGGTTTATGAGGTTTTAGTCATGGCACCTGCGGGt                                   >  2:312999/1‑149 (MQ=255)
                                                                                                                   gATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGCCGCCCCCCAAAAACCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTCATGACAGGGGAATTATGATGTTAAATTGATGGCTCCTGCCGGTgg                                 >  2:313092/1‑148 (MQ=255)
                                                                                                                     tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccc                               <  1:312949/149‑1 (MQ=255)
                                                                                                                             ttCCGAAAGTTATCCGGGCCCGGGCGCCGCCCCCAAATATACTTCAGATGATGAGTGGTGGTGGATTAGGGTATGGGAAGGTGTTCAGATTTTATTAACAGGGGAATTATGATGTTTAATTGATGGCACCTGCCGGTGCCCCCCTTTGt                       >  2:313004/1‑149 (MQ=255)
                                                                                                                             ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAcc                                          <  1:312952/130‑1 (MQ=255)
                                                                                                                             ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTg                        <  1:312951/148‑1 (MQ=255)
                                                                                                                              tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                      <  2:312953/149‑1 (MQ=255)
                                                                                                                               ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                     <  2:312956/149‑1 (MQ=255)
                                                                                                                               ccGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                     <  2:312954/149‑1 (MQ=255)
                                                                                                                               ccGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATACTGGATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGTATTATGATGTTTAATGAATGGCACCTGCCGGTGCCCCCAGTTGTTa                     >  2:313021/1‑149 (MQ=255)
                                                                                                                                  aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                  <  1:312959/149‑1 (MQ=255)
                                                                                                                                     gTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                <  1:312960/148‑1 (MQ=255)
                                                                                                                                       tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc             <  1:312963/149‑1 (MQ=255)
                                                                                                                                       tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc             <  1:312962/149‑1 (MQ=255)
                                                                                                                                          ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa          <  2:312964/149‑1 (MQ=255)
                                                                                                                                            gCCCCGGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTTTGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTATACAAAGCAAAGa        <  2:312967/149‑1 (MQ=255)
                                                                                                                                            gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa        <  2:312969/149‑1 (MQ=255)
                                                                                                                                            gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa        <  2:312966/149‑1 (MQ=255)
                                                                                                                                              ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa           >  2:313062/1‑144 (MQ=255)
                                                                                                                                              ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc      <  1:312970/149‑1 (MQ=255)
                                                                                                                                              ccccGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa           >  2:313055/1‑144 (MQ=255)
                                                                                                                                               cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa     >  2:313037/1‑149 (MQ=255)
                                                                                                                                               cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa     >  1:313061/1‑149 (MQ=255)
                                                                                                                                                  gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  <  2:312972/149‑1 (MQ=255)
                                                                                                                                                  gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  >  2:313071/1‑149 (MQ=255)
                                                                                                                                                  gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  >  1:313041/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACT  >  U00096/422282‑422576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑