New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	2213706 =	206 (1.050)	3 (0.020)	3/288	27.2	1.5%	coding (939/1686 nt)	btsS	high‑affinity pyruvate receptor
	?	U00096	= 3125261	189 (0.970)					coding (199/1224 nt)	glcF	glycolate dehydrogenase, putative iron‑sulfur subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACGACCACCATTTACCCGGCAAACCGATTTCTTCAACTTATACTTTAAAAGCGATTGAAACCGGTGAAGTGGTCTACGCTGATGGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGCAATGCAAACTGGGGTCGACGCTGGTAATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  U00096/2213854‑2213706 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGTGATATTGTCGAGCAGAAAGTGAAACGCCCACTGCCGGAGCGAATACTGC  <  U00096/3125261‑3125114                                                                                                                                                                                                                                                                                                           ACGACCACCATTTACCCGGCAAACCGATTTCTTCAACTTATACTTTAAAAGCGATTGAAACCGGTGAAGTGGTCTACGCTGATGGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGCAATGCAAACTGGGGTCGACGCTGGTAATT                                                                                                                                                      >  2:1458054/1‑149    acaACCATTTACCAGGCAAACCGATTTCTTCAACTTATACTTTAAAAGCGATTGAAACCGGTGAAGTGGTCTACGCTGATGGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGAAATGCAAACTGGGGTCGACGCTGGTAATTcag                                                                                                                                                   >  1:1458053/4‑146    ACCACCATTTACCCGGCAAACCGATTTCTTCAACTTATACTTTAAAAGCGATTGAAACCGGTGAAGTGGTCTACGCTGATGGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGCAATGCAAACTGGGGTCGACGCTGGTAATTAcg                                                                                                                                                   >  1:1458052/1‑147                                     CTTATACTTTAAAAGCGATTGAAACCGGTGAAGTGGTCTACGCTGATGGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGCAATGCAAACTGGGGTCGACGCTGGTAATTAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGC                                                                                                                  >  2:2087494/1‑149                                                                                    GGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGCAATGCAAACTGGGGTCGACGCTGGTAATTAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTT                                                                   <  1:2087494/149‑1                                                                                                                                                    TTAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGT                                                   >  1:2087488/1‑101                                                                                                                                                     TAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGTGCGTGATATTGTCTAGCAGAAAGTGAAACGCCCACTGCCGGAGCGAATACTGC  <  1:2087559/149‑1                                                                                                                                                     TAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGTGATATTGTCGAGCAGAAAGTGAAACGCCCACTGCCGGAGCGAATACTGC  >  2:2087449/1‑149                                                                                                                                                     TAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGTGATATTGTCGAGCAGAAAGTGAAACGCCCACTGCCGGAGCGAATACTGC  >  2:2087450/1‑149                                                                                                                                                                                                                                                                                                           ACGACCACCATTTACCCGGCAAACCGATTTCTTCAACTTATACTTTAAAAGCGATTGAAACCGGTGAAGTGGTCTACGCTGATGGCAACGAAGTACCTTACCGTTGCTCTTTGCATCCGCAATGCAAACTGGGGTCGACGCTGGTAATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  U00096/2213854‑2213706 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttAAAACACAGGAGCATCTCGATCGCTGCCTCACTTGCCGTAATTGTGAAACCACCTGTCCTTCTGGTGTGCGCTATCACAATTTGCTGGATATCGGGCGTGATATTGTCGAGCAGAAAGTGAAACGCCCACTGCCGGAGCGAATACTGC  <  U00096/3125261‑3125114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.