Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG21.1% 282.6 / 18.0 134intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (16/89);  new base G (26/2);  total (43/91)
Fisher's exact test for biased strand distribution p-value = 1.53e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACT  >  U00096/422285‑422576
                                                                                                                                                |                                                                                                                                                   
gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGgccgc                                                                                                                                                 >  2:275543/1‑147 (MQ=255)
gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctc                                                                                                                                                 <  2:275497/149‑1 (MQ=255)
  gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGgccgccg                                                                                                                                               >  2:275643/1‑148 (MQ=255)
       ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTccccc                                                                                                                                          <  1:275498/149‑1 (MQ=255)
       ttgttgTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGccccc                                                                                                                                          >  1:275651/1‑149 (MQ=255)
        tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcct                                                                                                                                               <  1:275499/143‑1 (MQ=255)
         gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCaa                                                                                                                                        >  2:275574/1‑149 (MQ=255)
          ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaaa                                                                                                                                       <  2:275500/149‑1 (MQ=255)
           tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCCGCCCCCaaaa                                                                                                                                      >  2:275567/1‑148 (MQ=255)
           tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGATATCCCCCCCGGGCCTCCGCCCCCa                                                                                                                                         >  2:275630/1‑146 (MQ=255)
                tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                 >  2:275558/1‑149 (MQ=255)
                   tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGCCGCCGCCCCCAAATATCCTTCa                                                                                                                              >  2:275526/1‑149 (MQ=255)
                    gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAg                                                                                                                             >  1:275586/1‑149 (MQ=255)
                     aTTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                            <  2:275504/149‑1 (MQ=255)
                     aTTGGCTGGAGGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAga                                                                                                                            <  2:275503/149‑1 (MQ=255)
                      ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgat                                                                                                                           <  1:275505/149‑1 (MQ=255)
                           tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                      <  2:1439172/149‑1 (MQ=255)
                            ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCGGCGCCCCCCCATATCCTGGAGCTGATGAg                                                                                                                     >  1:275585/1‑149 (MQ=255)
                            ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAg                                                                                                                     <  2:275508/149‑1 (MQ=255)
                              aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                   <  1:275510/149‑1 (MQ=255)
                                  ttAAACGCCGCCGCGATCGTCAGCTGGCGGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                               <  2:275511/149‑1 (MQ=255)
                                     aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                            <  1:275513/149‑1 (MQ=255)
                                     aaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                            <  2:275514/149‑1 (MQ=255)
                                       cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                                          <  1:275515/149‑1 (MQ=255)
                                         ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                        <  1:275517/149‑1 (MQ=255)
                                           gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                      <  2:275518/149‑1 (MQ=255)
                                           gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                      <  2:275519/149‑1 (MQ=255)
                                            ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCCTCCTCCCCCCAATATCCGTAAGATGGTGAGAGGGGCTGCGTTAggg                                                                                                     >  2:275659/1‑148 (MQ=255)
                                            ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGTCCTCCCCCACATATAGGTCAGATGAGGAGTGATCCTGCGTTAGGc                                                                                                     >  2:275627/1‑149 (MQ=255)
                                             cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGGTCTGTAAGCCGGTTCCGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCt                                                                                                    <  2:275520/149‑1 (MQ=255)
                                              aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCGCCGCCCCCACATCGACTGAAGAAGAGGAGGGATCCAGGATGAGGCTa                                                                                                   >  2:275652/1‑149 (MQ=255)
                                                gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGGCGGGCGCCGCCCCCAACTATCGGTGAGATGATGAGTGATCGTGCGTTAggggatg                                                                                                 >  2:275592/1‑144 (MQ=255)
                                                gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                       <  1:275522/143‑1 (MQ=255)
                                                 aTCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTATGTAAGCCGGTTCAGTAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                <  1:275524/149‑1 (MQ=255)
                                                  tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                               <  1:275526/149‑1 (MQ=255)
                                                    gTCAGGTGGGTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCATATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                   <  1:275527/143‑1 (MQ=255)
                                                    gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGCCGTGCGCCGCCCCCAAGAAAGCGACGTATGGTGAGTGATCCTGCAATTGGCTATGGCaa                                                                                             >  2:275648/1‑149 (MQ=255)
                                                     tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                            <  1:275528/149‑1 (MQ=255)
                                                     tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTTGGCTATGGGAAg                                                                                            >  1:275649/1‑149 (MQ=255)
                                                       aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGGCCACCTCACCCAAATAAAATTCAGATGATGAGTGATGCTGAATTATGCTATGGaaaatt                                                                                          >  2:275589/1‑146 (MQ=255)
                                                        gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                         <  2:275530/149‑1 (MQ=255)
                                                           ggcttAAAACCAGTAATCCTTGCTTTCTGTAAGCCGTTTCAGGAGGAAATCCTGATCCGGCTAATCCGAAAGTAATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAACCTGCATTAGGCTATGGCAAGGTGATc                                                                                      <  1:275531/149‑1 (MQ=255)
                                                           ggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCTGCTCCCCCAACAATACTTCACAAGATGAGTGATCATGCAATTGGGTATGGCaatgagctc                                                                                      >  2:275591/1‑142 (MQ=255)
                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                     <  1:275532/149‑1 (MQ=255)
                                                            gctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCACCTCCCCCCAATATCCATCAGAAGATGAGTGATACTGCATTAGGCAATTGCAAGGTGATCa                                                                                     >  2:275584/1‑149 (MQ=255)
                                                                aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                 <  2:275534/149‑1 (MQ=255)
                                                                    ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                             <  2:275535/149‑1 (MQ=255)
                                                                      aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGGCCCCAGATATCCTGGAGATGATGAGGGAGCCTGCGGTATGCTAGGGCAAGGTGATCAGATGTtcatc                                                                           >  2:275640/1‑149 (MQ=255)
                                                                       gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcatca                                                                          <  1:275537/149‑1 (MQ=255)
                                                                         aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCGCCTCCCCCAAATATCCTACAGATGATGAGTGATCATGCATTAGGCTATGGCAAGGTGATGAGATTTTAATcaca                                                                        >  2:275663/1‑149 (MQ=255)
                                                                          aTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                       <  2:275538/149‑1 (MQ=255)
                                                                           tCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                      <  1:275539/149‑1 (MQ=255)
                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCGCCCACAAATATCATTCAGATGATGATTGATCGTGGATTAGGCTATGGCAAGGTGATAAGATTTTCTGGAAAGGGGaa                                                                  >  2:275614/1‑149 (MQ=255)
                                                                               tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                  <  1:275541/149‑1 (MQ=255)
                                                                                gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                 <  2:275542/149‑1 (MQ=255)
                                                                                  gTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAGGGTGGTGAGGTTTTGAGAGCAGGGGAATTa                                                               >  2:275620/1‑149 (MQ=255)
                                                                                   ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAGATCCTGCGGATGATGGGTGAGCCTGCATTAGGGTATGGCAAGGTGGTGGGGTTTTCATCACAGGGGAATTat                                                              >  2:275632/1‑149 (MQ=255)
                                                                                      tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                           <  1:275544/149‑1 (MQ=255)
                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTAAGATGATGAGGGAGCCTGCAGTTGGCTATGGGGTGGGGAGGAGATTTTCTTGACAGGGGAATTatgatg                                                          >  2:275670/1‑149 (MQ=255)
                                                                                        tAAGCGGGATCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                         <  1:275545/149‑1 (MQ=255)
                                                                                         aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGTTGAGATTTTGATGACAGGGGGATTTTgttgtt                                                        >  2:275634/1‑149 (MQ=255)
                                                                                               gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                  <  2:275548/149‑1 (MQ=255)
                                                                                                ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                 <  2:275549/149‑1 (MQ=255)
                                                                                                   aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                              <  2:275550/149‑1 (MQ=255)
                                                                                                    ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                             <  1:275551/149‑1 (MQ=255)
                                                                                                        gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                         <  1:275552/149‑1 (MQ=255)
                                                                                                        gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCACACCCAAAAAACATTCAGATGATTAGTGATACAGCATTAGACAAAGCAAAGGTGTTAAGATATTAAAAACAAGGGAATTAAGATGATAAATGAAttgaacaa                                         >  2:275618/1‑142 (MQ=255)
                                                                                                         aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCt                                         <  1:275553/148‑1 (MQ=255)
                                                                                                          aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                       <  2:275555/149‑1 (MQ=255)
                                                                                                           aTCCTGATCCGGCTATTCCGAATGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATCGAAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATTGCACCTGcc                                      <  2:275556/149‑1 (MQ=255)
                                                                                                             ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                    <  2:275559/149‑1 (MQ=255)
                                                                                                                     ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                         <  1:275560/120‑1 (MQ=255)
                                                                                                                     ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                            <  1:275561/149‑1 (MQ=255)
                                                                                                                      gCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCCAAAACCTGCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGAGCAGATTTTAATGACAGGGGATTTTTGATGTTAAATGAATGGCACCAGCCGGGGCCCCCaa                           >  2:275704/1‑148 (MQ=255)
                                                                                                                       cTATTCCGAAAGTTATCCGGGGCCGGCCGGCGCCCCCAAAGATAGGTCAGGTGATGGGTGATGCGGCATTAGGGTGTGGGGAGGTGGTGGGTTTTTGTTGACAGGGGATTTATGTTGTTTTATGCATGGCGCGGGGCGGTGCGCCCagt                          >  2:275681/1‑147 (MQ=255)
                                                                                                                       cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                            <  1:275564/147‑1 (MQ=255)
                                                                                                                       cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                          <  1:275563/149‑1 (MQ=255)
                                                                                                                        tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                         <  1:275565/149‑1 (MQ=255)
                                                                                                                         aTTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAATAACATTGAGATGAGGAGTGATCCTGGAGTAGGCGATGGAAAGGTGATAAGAGTTTCATAAAAAGGGAGTTATGATGTTTAATGAATGGCACCGGCCGGGGCCCCCaggtg                        >  2:275688/1‑145 (MQ=255)
                                                                                                                           tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCTAGGTGATCAGATTTTCATCACAGTGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                      <  1:1270582/149‑1 (MQ=255)
                                                                                                                             cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                    <  1:275567/149‑1 (MQ=255)
                                                                                                                              gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGAGCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaaa                   <  1:275568/149‑1 (MQ=255)
                                                                                                                                aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                 <  2:275571/149‑1 (MQ=255)
                                                                                                                                 aGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaa                <  1:275572/149‑1 (MQ=255)
                                                                                                                                  gTTATCCGCCCCCGTCCTCCTACCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTATGCTATTGCAATGTGATCAGATTTTCCTCACAGGGGAATTATGATGTTAAATGCATGTCACCTGCCGGTGCCCCCATTTGTTAAAcaaa               <  1:275573/149‑1 (MQ=255)
                                                                                                                                   ttATCCGCGCCCGTCCGCCGCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCAAAGGGGAATTATTATGTTAAATTAATGGCACCTGCCGCGGCCCCCAGATGTTAAAcaaag              >  2:275647/1‑149 (MQ=255)
                                                                                                                                   ttATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag              <  1:275574/149‑1 (MQ=255)
                                                                                                                                      tCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa           <  2:275578/149‑1 (MQ=255)
                                                                                                                                      tCCGCCCCCGGCCTCCTCACCCAAATATCCTTCAGATGATTAGTGCTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATCCATGGCACCTTCCTGTGCCCCCAATTGTTAAAcaaagcaa           <  2:275577/149‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa          <  2:275579/149‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa          <  1:275581/149‑1 (MQ=255)
                                                                                                                                         gCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa        <  2:275582/149‑1 (MQ=255)
                                                                                                                                         gCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa           >  2:275636/1‑146 (MQ=255)
                                                                                                                                          cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca            <  1:275583/144‑1 (MQ=255)
                                                                                                                                          cacccGTCCTACTCACCCAAATATCATTAAGATGATGAGTGATACTGCATTAGGGTATGGCAAGTTGATAAGATTTTAATAAAAGGGGAATTATGATGTTAAATGAATTGCAACTGCCGGTGCCCCCATTTGTTAAAaaaagcaa           >  2:275677/3‑145 (MQ=255)
                                                                                                                                              cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc   <  1:275588/149‑1 (MQ=255)
                                                                                                                                              cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc   <  2:275587/149‑1 (MQ=255)
                                                                                                                                              cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc   <  2:275586/149‑1 (MQ=255)
                                                                                                                                               gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCGTCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  >  1:275727/1‑149 (MQ=255)
                                                                                                                                               gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  >  1:275682/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACT  >  U00096/422285‑422576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑