Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG22.2% 404.5 / 25.7 181intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (17/123);  new base G (36/4);  total (54/127)
Fisher's exact test for biased strand distribution p-value = 1.85e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 193 total aligned reads displayed.

TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  U00096/422281‑422577
                                                                                                                                                    |                                                                                                                                                    
tttCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGt                                                                                                                                                      <  2:386277/149‑1 (MQ=255)
  tCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgcc                                                                                                                                                    >  2:386350/1‑149 (MQ=255)
    gcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGacctc                                                                                                                                                  >  2:386390/1‑149 (MQ=255)
     cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                 <  2:386280/149‑1 (MQ=255)
     cgTGGATTGTAGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACCATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcctcc                                                                                                                                                 <  2:386281/149‑1 (MQ=255)
                gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAata                                                                                                                                      <  1:386283/149‑1 (MQ=255)
                 tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAatat                                                                                                                                     <  2:386284/149‑1 (MQ=255)
                  gCTGTTGAGTGGCTGGATGTTTAAACGCCGCCACGAGCGTCAGCTGGCTGCAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                    <  1:386285/149‑1 (MQ=255)
                     gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGGCTCCCCCACATATCCtt                                                                                                                                 >  2:386421/1‑149 (MQ=255)
                       tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                               <  1:386288/149‑1 (MQ=255)
                        gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                              <  2:386289/149‑1 (MQ=255)
                           tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatg                                                                                                                           <  2:386292/149‑1 (MQ=255)
                             gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGACCTACACCCCCAAATATCCTTCAgctgat                                                                                                                         >  2:386441/1‑149 (MQ=255)
                              cTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGatgatg                                                                                                                        <  1:386295/149‑1 (MQ=255)
                               tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                       <  1:386297/149‑1 (MQ=255)
                                ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAg                                                                                                                      >  2:386446/1‑149 (MQ=255)
                                    gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                  <  1:386299/149‑1 (MQ=255)
                                      ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                                <  2:386302/149‑1 (MQ=255)
                                      ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                                <  2:386300/149‑1 (MQ=255)
                                        aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCACCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                              <  1:386305/149‑1 (MQ=255)
                                         aaagcctccACGATCGTCAGCTGGCTGAACACCAGTAATCCTTGCGTTATTTAAGCCGGTTCAGTCTGAAATCCTGCTCCGGCTATTCCGAAAGTTATCCGCCCCCTGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                             <  2:386306/146‑1 (MQ=255)
                                            gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                          <  1:386309/149‑1 (MQ=255)
                                             ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                         <  2:386310/149‑1 (MQ=255)
                                               gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCTCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                       >  1:386438/1‑149 (MQ=255)
                                               gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                        <  1:386312/148‑1 (MQ=255)
                                                  aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                    <  2:386314/149‑1 (MQ=255)
                                                   cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTTTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                   <  1:386318/149‑1 (MQ=255)
                                                   cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                   <  2:386320/149‑1 (MQ=255)
                                                   cGATCGTCAGCTAGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                   <  2:386316/149‑1 (MQ=255)
                                                      tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                <  1:386322/149‑1 (MQ=255)
                                                        gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                              <  2:386326/149‑1 (MQ=255)
                                                           aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                              <  1:386328/146‑1 (MQ=255)
                                                           aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                           <  2:386329/149‑1 (MQ=255)
                                                           aGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGt                                                                                           <  1:386331/149‑1 (MQ=255)
                                                                   gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGCGGGGCGGCGGCCCCCCAGAGCCGGCAGATGATGAGTGATCCTGGAGGAGGGTATGGGAAGGTGATCAGag                                                                                   >  2:386468/1‑148 (MQ=255)
                                                                   gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGGCGGGCGCCGCGCCCACAGAGCAAGGAGAGGAAGAGGGATCCGGAAGTAGGGTATGGCAAGGTgggccgag                                                                                   >  2:386487/1‑142 (MQ=255)
                                                                    aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                   <  1:386335/148‑1 (MQ=255)
                                                                    aaaaCCAGTAATCCTTGCGTTCTCTACGCCGGTTCAGGAGGTAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                  <  2:386334/149‑1 (MQ=255)
                                                                     aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATCCTTAAGCTGATGAGTGATCCTGAATTAGGCTATGGGAAGGTGATGAGAgtt                                                                                 >  2:386407/1‑149 (MQ=255)
                                                                      aaCCAGTACTCCTTGCGGTATGTAAGCCTGGTCAGGAGGAAATCCTGTTCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAACTATCCTTCAGATGATGAGGGATCCTGCATTAGGCGATGGCAAGGTGATCAGAtttt                                                                                <  2:386337/149‑1 (MQ=255)
                                                                      aaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCACCCCCAACAAACCATAAGATGATGAGTGATCGTGAATTTGGCTATGGCAAGGTGATCAGAtttt                                                                                >  2:386493/1‑149 (MQ=255)
                                                                        ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtca                                                                              <  2:386338/149‑1 (MQ=255)
                                                                         cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                             <  1:386339/149‑1 (MQ=255)
                                                                            tAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAACCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                          <  1:386341/149‑1 (MQ=255)
                                                                               tCCTTGCGTTCTGTAAGCCGGTTAAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATTGCAAGGTGATCAGATTTTCATCACAgg                                                                       <  1:386343/149‑1 (MQ=255)
                                                                                ccTTGCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                      <  2:386344/149‑1 (MQ=255)
                                                                                  ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTCTCCGCCGCCGGCCGGCGCCCCCAAATATACGTCAGCTGATGAGTGATGCTGCAGTAGGCTATGGGAAGGTGGGCAGATTTTCATCAGGGGGGa                                                                    >  2:386481/1‑149 (MQ=255)
                                                                                   tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGCCCGGCCGCGGCCCCCAAATTTCCTTCAGATGAGGAGTGCTCCGGCATTAGGGGATGGCTAGGGGTGCAGGGTTTCATGTCAGGGGaa                                                                   >  2:386357/1‑149 (MQ=255)
                                                                                   agCGTTCTGTAAGCCGGTGCAGGAGGAAATCCTGATCCGGCTATTCCGAACCTGATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGACGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                   <  2:386348/148‑1 (MQ=255)
                                                                                    gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                  <  1:386350/149‑1 (MQ=255)
                                                                                    gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAt                                                                  <  2:386352/149‑1 (MQ=255)
                                                                                         cTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                             <  2:386354/149‑1 (MQ=255)
                                                                                          tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                            <  1:386356/149‑1 (MQ=255)
                                                                                          tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgat                                                            <  1:386358/149‑1 (MQ=255)
                                                                                            tAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                          <  2:386360/149‑1 (MQ=255)
                                                                                             aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                         <  2:386362/149‑1 (MQ=255)
                                                                                              aGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTa                                                        <  1:386363/149‑1 (MQ=255)
                                                                                               gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                       <  2:386365/149‑1 (MQ=255)
                                                                                                 cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                     <  1:386368/149‑1 (MQ=255)
                                                                                                 cGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                     <  2:386367/149‑1 (MQ=255)
                                                                                                  ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATg                                                    <  1:386370/149‑1 (MQ=255)
                                                                                                     tccggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                 <  1:386373/146‑1 (MQ=255)
                                                                                                     tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGGCCGGCCGCCGCCCCCCAATATACATCAGATGATGAGTGATGCGGCATTAGGCTAGGGCAAGGTGATCCGATTTTCTTCACAGGGGAATATTGATGTTaaaatcat                                                 >  2:386447/1‑144 (MQ=255)
                                                                                                     tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGGAAGGTGGTCAGATTTTAATCACAGGGGAATTATGATGTTAAATGCAt                                                 >  2:386434/1‑149 (MQ=255)
                                                                                                       aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                               <  2:386375/149‑1 (MQ=255)
                                                                                                             aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATACGTCAGCTGATGAGTGATGCTGCATTAGGCTATGGCGAGGTGCTCAGATTTTCAGAACAGGGGAAGTATGTTGTTTAATGCATggaaacgg                                         >  2:386506/1‑143 (MQ=255)
                                                                                                              aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGTTCCTGCATTAGGCTATGGCGAGGTGTTGAGATTTTAATAGAAGGGGAATTTTGTTGTTAAATGCATGGCACCAGc                                        >  2:386466/1‑149 (MQ=255)
                                                                                                              aaTCCTGATACGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTTATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                        <  1:386377/149‑1 (MQ=255)
                                                                                                                tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATACGTCAGATGATGAGTGATGCTGCATTAGGCTATGGGAAGGTGATCAGATTTTGATCACAGGGGAATTATGATGTTTAATGAATGGCCCCGGCCg                                      >  2:386500/1‑149 (MQ=255)
                                                                                                                 ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                     <  2:386379/149‑1 (MQ=255)
                                                                                                                   tGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                   <  2:386381/149‑1 (MQ=255)
                                                                                                                         ggCTATTCCGAAAGTTCTCCGCCCGCGGCCTCCGCCCCCCAATATCCTTCAGATGATGAGTGATCCTGCAGTAGGCTATGGCGGGGGGCTCGGAGTTTGATGACAGGGGGATGATGATGTTAAATTCATGGCACCGGCCGGGGCCCCCa                             >  2:386556/1‑149 (MQ=255)
                                                                                                                         ggCTATTCCGAAAGTTATCCGCCCCCCGCCACCGCCCCCCAAAATACGTCATAGGATGATTTATCCTGCATTAGGCAAAGGGAAGGTGATCAGATTTTAATAACATGGGAATTATGATTTTAAATGCATTGCACCTGCCGGGGCACCCa                             >  2:386560/1‑149 (MQ=255)
                                                                                                                          gCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAGTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGccccctt                            <  2:386382/149‑3 (MQ=255)
                                                                                                                           cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                           <  1:386383/149‑1 (MQ=255)
                                                                                                                            tATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                          <  2:386385/149‑1 (MQ=255)
                                                                                                                              ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGt                        <  2:386387/149‑1 (MQ=255)
                                                                                                                               tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGtt                       <  1:386388/149‑1 (MQ=255)
                                                                                                                                ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                      <  1:386390/149‑1 (MQ=255)
                                                                                                                                 cGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                     <  1:386392/149‑1 (MQ=255)
                                                                                                                                   aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                   <  2:386393/149‑1 (MQ=255)
                                                                                                                                   aaaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                   <  1:386394/149‑1 (MQ=255)
                                                                                                                                    aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                  <  2:386397/149‑1 (MQ=255)
                                                                                                                                    aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                  <  1:386396/149‑1 (MQ=255)
                                                                                                                                      gTTATCCGCCCCCGTCCTCCTCCCCCAAAAATCCATCAGATGATGAGTGATACTGCATTAGGCTATGGCAACGTGATCAGATTTTAATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGGGCCCCCAATTGATAAAcaaa                >  1:386588/1‑149 (MQ=255)
                                                                                                                                         aTCCGCCCCCGGCCGCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca             >  2:386478/1‑149 (MQ=255)
                                                                                                                                           ccGCCCCCGTCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa           <  1:386400/149‑1 (MQ=255)
                                                                                                                                            cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag          <  2:386402/149‑1 (MQ=255)
                                                                                                                                            cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag          <  1:386403/149‑1 (MQ=255)
                                                                                                                                              cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            >  2:386498/1‑145 (MQ=255)
                                                                                                                                               ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa            >  2:386525/1‑144 (MQ=255)
                                                                                                                                               ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc       <  2:386405/149‑1 (MQ=255)
                                                                                                                                                cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa      >  1:386465/1‑149 (MQ=255)
                                                                                                                                                 ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACACAGCAAAGATCa      <  1:386408/148‑1 (MQ=255)
                                                                                                                                                 ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa      >  1:386518/1‑148 (MQ=255)
                                                                                                                                                 ccGTCATACTCCGCCAAATATCCTTCAGATGATGATTGATCCTGCATTTGGCTATGGTAAGGTGTTCAGATTTTCATCACAGTGGAATTTTGATGTTATTTGCATGGCTCGTGCCGTTGACGCGTTTTGTTTAAGAAATTAAAGATcta     <  2:386410/149‑3 (MQ=255)
                                                                                                                                                  cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa     <  1:386411/148‑1 (MQ=255)
                                                                                                                                                   gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt   >  1:386496/1‑149 (MQ=255)
                                                                                                                                                    tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  >  1:386460/1‑149 (MQ=255)
                                                                                                                                                    tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg  >  2:386549/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTG  >  U00096/422281‑422577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑