BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	1,552,191	0	C	A	24.5%	319.1 / 24.5	148	intergenic (+200/+207)	fdnI/yddM	formate dehydrogenase N subunit gamma/DNA‑binding transcriptional regulator YddM
Reads supporting (aligned to +/- strand): ref base C (32/79); new base A (29/7); total (62/86)
Fisher's exact test for biased strand distribution p-value = 5.26e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 151 total aligned reads displayed.

TATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGC  >  U00096/1552043‑1552338

tatTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTc                                                                                                                                                     <  2:1413885/149‑1 (MQ=31)

 atTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa                                                                                                                                                    <  1:1413888/149‑1 (MQ=32)

 atTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTAAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCa                                                                                                                                                    <  1:1413886/149‑1 (MQ=17)

   tGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCaaa                                                                                                                                                  <  2:1413890/149‑1 (MQ=33)

     tAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAATTg                                                                                                                                                <  1:1413891/149‑1 (MQ=17)

     tAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGGGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATg                                                                                                                                                <  1:1413892/149‑1 (MQ=21)

       gACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgc                                                                                                                                              <  2:1413894/149‑1 (MQ=34)

        aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgc                                                                                                                                              <  1:1413896/148‑1 (MQ=34)

            cGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGt                                                                                                                                         <  2:1413897/149‑1 (MQ=35)

              tAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCGAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaa                                                                                                                                       <  1:1413898/149‑1 (MQ=38)

              tAGGCTTTGTTCATGCCGGATGCGGCGTGAAAGCCTTATCCGGCATGAAAACCCTTAAAATACAATAGATTGCTGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGGAATGTTGCGGTTGTCATCAGTTTCAAATGGCGCTGTaa                                                                                                                                       >  2:1413943/1‑149 (MQ=11)

               aGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaaa                                                                                                                                      <  1:1413900/149‑1 (MQ=38)

               aGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTaaa                                                                                                                                      <  1:1413901/149‑1 (MQ=38)

                 gCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAg                                                                                                                                    <  2:1413903/149‑1 (MQ=40)

                  cTTTGTTCATTCCGGATTCTTCTTGAACGCCTTATCCGGAATGAAAACCCTTCAAATCCACTAGATTGCAGTGAACGTGTAGTCCTGATATGCGTAGCGCATCAGTCAATGTTTCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAgg                                                                                                                                   <  2:1413905/149‑1 (MQ=14)

                  cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAgg                                                                                                                                   <  1:1413906/149‑1 (MQ=40)

                   tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGc                                                                                                                                  <  2:1413909/149‑1 (MQ=255)

                   tttGTTCATGCCGGATGCGGCGTGAACGACTTAACCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGc                                                                                                                                  <  1:1413908/149‑1 (MQ=255)

                       ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCa                                                                                                                              >  2:1413949/1‑149 (MQ=255)

                       ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCa                                                                                                                              >  2:1413957/1‑149 (MQ=255)

                       ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCa                                                                                                                              <  1:1413911/149‑1 (MQ=255)

                          aTGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTTCGTTTTTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                                                                                                                           <  2:1413912/149‑1 (MQ=255)

                           tGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCAttt                                                                                                                           <  1:1413913/148‑1 (MQ=255)

                            gCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGACCGTGTAGGCCTGATAAGCGGAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTc                                                                                                                         <  2:1413916/149‑1 (MQ=255)

                             ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCa                                                                                                                        <  2:1413917/149‑1 (MQ=255)

                             ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCa                                                                                                                        <  1:1413918/149‑1 (MQ=255)

                               ggATGCGGCGTGAACGCCTTATCCGGCATGAATACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCata                                                                                                                      <  2:1413920/149‑1 (MQ=255)

                                gATGCGGCGTGAACGCCTTATCCGGCATGAAAAGCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCTCATCAGGCAATGTTGCGTTTGTCATCAGTTGCAAATGGCGCTGTAAAAGGCGTCATTTTCatat                                                                                                                     <  2:1413922/149‑1 (MQ=255)

                                 aTGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATAtt                                                                                                                    <  2:1413923/149‑1 (MQ=255)

                                    cggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGTCCTGATAAGCGTAGCTCATCAGGCAATGTTGCGTTAGTCATCAGATTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTa                                                                                                                 <  2:1413925/149‑1 (MQ=255)

                                       cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAc                                                                                                              <  1:1413927/149‑1 (MQ=255)

                                       cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGAc                                                                                                              <  2:1413926/149‑1 (MQ=255)

                                          gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAAc                                                                                                           <  2:1413930/149‑1 (MQ=255)

                                          gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAAc                                                                                                           <  2:1413931/149‑1 (MQ=255)

                                          gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGTCATTTTAATATTGAATACAAc                                                                                                           >  2:1414052/1‑149 (MQ=14)

                                              gCCTTATCCGGCATGAATACCCTTCAAATCCAATAGATTTCAGTGAACGTGTAGGCCTGATAAGCGGAGCGCATCAGGCAATGTTGCGTTTGTCATCAGGTGCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAg                                                                                                       <  2:1413933/149‑1 (MQ=18)

                                               ccTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGACATTTTAATATTGTATAAAACGTAgg                                                                                                      >  2:1414090/1‑149 (MQ=14)

                                                    tCCGGCATGAAAAACCTTCAAATCCAATAGATTTCAGTGAACGTGTAGGCCTGATAAGCGTAGCTCATCAGGCAATGTTTCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTg                                                                                                 <  2:1413934/149‑1 (MQ=18)

                                                           nnaaaaCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGAGGCATTTTCATATTGTTGACAACGTAGGCTTTGTTCATGc                                                                                          >  2:1414108/3‑149 (MQ=255)

                                                            gAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGcc                                                                                         >  2:1413973/1‑149 (MQ=255)

                                                            gAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTTTAAAAGGAGTCATTTTAATATTGAATACAACGTAGGCTTAGTACATGcc                                                                                         >  2:1414137/1‑149 (MQ=17)

                                                            gAAAACCCTTCAAATCC‑ATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGAGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCg                                                                                        >  2:1414041/1‑149 (MQ=255)

                                                             aaaaCGCTTCATATCCAATAGATTGCATTGAACGTGTAGGCCTGTTATGCGTAGCGCTTCAGTTAATGTTGAGTTTTTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCg                                                                                        <  2:1413938/149‑1 (MQ=25)

                                                              aaaCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGTCATTTTAATATTGTATACACAGTAGGCTTTCTACATGCCgg                                                                                       >  2:1414104/1‑149 (MQ=17)

                                                                    ttCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGgcgc                                                                                                                                            <  1:1413940/90‑1 (MQ=38)

                                                                    ttCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATgcgg                                                                                 <  1:1413941/149‑1 (MQ=255)

                                                                         aTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGa                                                                            <  1:1413945/149‑1 (MQ=255)

                                                                             aaTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGc                                                                        >  2:1413965/1‑149 (MQ=255)

                                                                              aTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACg                                                                         >  1:1413958/1‑147 (MQ=255)

                                                                              aTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                       <  1:1413946/149‑1 (MQ=255)

                                                                               tAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGGCAtttt                                                                                                                          >  1:1414086/1‑97 (MQ=255)

                                                                                tgATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                     <  2:1413947/148‑1 (MQ=255)

                                                                                       aGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTTAAATGGCGCTGTa                                                                                                                                        >  1:1414033/1‑75 (MQ=21)

                                                                                        gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                             <  2:1413951/149‑1 (MQ=255)

                                                                                         tGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                            >  2:1414007/1‑149 (MQ=255)

                                                                                          gAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATg                                                           <  2:1413952/149‑1 (MQ=255)

                                                                                            aCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTa                                                                                                                                        >  1:1414134/1‑70 (MQ=255)

                                                                                                gtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATAATGAGTCATTTTAATATTGTGGACCACGTAGGCTTTGTTCATGCCGTATGCGGCGTGATCGCCTTATCCGGGATGacaccc                                                     >  2:1414106/1‑144 (MQ=21)

                                                                                                  aGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCt                                                   <  2:1413954/149‑1 (MQ=255)

                                                                                                  aGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCt                                                   <  2:1413955/149‑1 (MQ=255)

                                                                                                   ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCtt                                                  <  1:1413957/149‑1 (MQ=255)

                                                                                                   ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCtt                                                  <  2:1413958/149‑1 (MQ=255)

                                                                                                      cTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCt                                                                                                                                           >  1:1414117/1‑57 (MQ=255)

                                                                                                       tGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGACTTATCCGGCATGAAAACCCTTCaaa                                              >  2:1414123/1‑149 (MQ=255)

                                                                                                        gATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCTTCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAAt                                             <  2:1413961/149‑1 (MQ=255)

                                                                                                        gATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGGCATTTTGATATTGGAGACAACGTCGGCTTTGTTCCTGCAGGTTGCGGCGTGAAAGACTTATCCGGCATGAAAACCCTGCAAAt                                             >  2:1414124/1‑149 (MQ=18)

                                                                                                            aGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTGCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCaa                                         <  2:1413963/149‑1 (MQ=255)

                                                                                                             gCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                        <  1:1413965/149‑1 (MQ=255)

                                                                                                             gCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                        >  2:1414166/1‑149 (MQ=255)

                                                                                                             gCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCt                                                                                                                                           >  1:1414118/1‑50 (MQ=255)

                                                                                                              cGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATa                                       <  2:1413966/149‑1 (MQ=255)

                                                                                                                  gctaaTCATGCAATGTAGCGTTTGCAATCAGCTGAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                   <  2:1413992/145‑1 (MQ=11)

                                                                                                                  gcgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                   <  2:1413969/149‑1 (MQ=255)

                                                                                                                  gcgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGAtt                                   <  2:1413970/149‑1 (MQ=255)

                                                                                                                    gcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGa                                     <  1:1413973/145‑1 (MQ=255)

                                                                                                                    gcATCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCt                                                                                                                                           >  1:1414138/1‑43 (MQ=255)

                                                                                                                      aTCAGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCt                                                                                                                                           >  1:1414164/1‑41 (MQ=255)

                                                                                                                         aGGCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCt                                                                                                                                           >  1:1414039/1‑38 (MQ=255)

                                                                                                                          ggCAATGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTATAAGGCGGCATTTTAATATTg                                                                                                                   >  1:1414128/1‑61 (MQ=17)

                                                                                                                             aaTGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGACTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgt                        >  2:1414111/1‑149 (MQ=255)

                                                                                                                             aaTGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACACTTCAAATCCAATAGATTGCAGTGAACgt                        >  2:1414153/1‑149 (MQ=255)

                                                                                                                             aaTGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGAGTCATTTTATTATTGTAGACAACGTCGGCTTTGTTCATGCCGGGTGAGGCGTGAGCGACTTGTACGGCGTGATAGCCCTTAAAATCCAATAAAATGCAGTggccgt                        >  2:1414058/1‑144 (MQ=255)

                                                                                                                              aTGTTGCGTTTGTCATCAGTTTAAAATGGCGCTGTAAAAGGAGTCATTTTAATATTGTAGACAACGTAGGCTTTGTTCATGACGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtg                       >  2:1414155/1‑149 (MQ=255)

                                                                                                                               tGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGGCATTTTCATATTGTTGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCGGAACCGGCCTGAAAACCCTTCAAATCAAATAGATTGCAGTGCACgtgt                      >  1:1414158/1‑149 (MQ=14)

                                                                                                                                    cGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGcc                 >  2:1414042/1‑149 (MQ=255)

                                                                                                                                     gTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGc                  >  1:1414105/1‑147 (MQ=255)

                                                                                                                                      tttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTg               >  1:1414129/1‑149 (MQ=255)

                                                                                                                                       ttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                      <  2:1413979/141‑1 (MQ=255)

                                                                                                                                       ttGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGa              >  1:1414056/1‑149 (MQ=255)

                                                                                                                                        tGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGAt             >  2:1414135/1‑149 (MQ=255)

                                                                                                                                          tcatcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCTGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATaa           >  1:1430337/1‑149 (MQ=255)

                                                                                                                                          tcatcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATa            >  1:1414049/1‑148 (MQ=40)

                                                                                                                                          tcatcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCGTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATaa           >  2:1414149/1‑149 (MQ=255)

                                                                                                                                           catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCatat                                                                                                                     <  1:1413980/42‑1 (MQ=255)

                                                                                                                                            atcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc         >  2:1414097/1‑149 (MQ=39)

                                                                                                                                              aaGTTTAAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGt       <  2:1413981/148‑1 (MQ=18)

                                                                                                                                                 tttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgc    >  1:1414205/1‑149 (MQ=35)

                                                                                                                                                  ttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCttt                                                                                                  <  1:1413984/54‑1 (MQ=255)

                                                                                                                                                   tCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcgc  >  2:1414157/1‑149 (MQ=33)

                                                                                                                                                    cAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGa                            <  2:1413974/122‑1 (MQ=33)

TATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGC  >  U00096/1552043‑1552338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`