Predicted mutation
evidence seq id position mutation annotation gene description
RA U00096 379,826 (C)5→4 coding (56/276 nt) frmR ← DNA‑binding transcriptional repressor FrmR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096379,8220C.100.0% 622.1 / NA 130coding (60/276 nt)frmRDNA‑binding transcriptional repressor FrmR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (56/74);  total (56/74)

Only 100 of 134 total aligned reads displayed.

GTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTG  >  U00096/379675‑379965
                                                                                                                                                   |                                                                                                                                               
gTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGcc                                                                                                                                                <  1:243311/149‑1 (MQ=255)
gTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGcc                                                                                                                                                >  2:243396/1‑149 (MQ=255)
    tttCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGccccga                                                                                                                                            >  1:243370/1‑147 (MQ=255)
     ttCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGccccgaa                                                                                                                                           <  2:243312/149‑4 (MQ=255)
       cGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATa                                                                                                                                        <  1:243313/149‑1 (MQ=255)
       cGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATa                                                                                                                                        >  2:243331/1‑149 (MQ=255)
        ggTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATac                                                                                                                                       >  2:243362/1‑149 (MQ=255)
          tCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATacga                                                                                                                                     <  2:243316/149‑1 (MQ=255)
           cAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGTCCGTTCCAGAGCATCAATCTG‑CCCCGAATtcgac                                                                                                                                    <  2:243317/149‑1 (MQ=255)
           cAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATacga                                                                                                                                     <  1:243318/148‑1 (MQ=255)
             aaCGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGACTAcgacga                                                                                                                                  >  2:243407/1‑149 (MQ=255)
             aaCGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATAcgacga                                                                                                                                  >  1:243337/1‑149 (MQ=255)
               cGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTGCCAGAGCATCAATCTG‑CCCCGAATACGACGAAc                                                                                                                                >  2:243406/1‑149 (MQ=255)
                gttaaccGGATATGGATTTCAAGCACATCTGCCATCAGCCCAATAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATCGCACGGCAATCGGCATCACCCTCCAGCGACCGTACCAGAGCATCAATCAG‑CCCCGAATACGACGAACt                                                                                                                               >  2:243373/6‑149 (MQ=255)
                 tttCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTc                                                                                                                              <  2:243320/149‑1 (MQ=255)
                 tttCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTc                                                                                                                              >  2:243379/1‑149 (MQ=255)
                  ttCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCg                                                                                                                             >  1:243367/1‑149 (MQ=255)
                    cccGGATATGGCTTTCAAGCGCTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAg                                                                                                                           <  2:243321/149‑1 (MQ=255)
                     ccGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGt                                                                                                                          <  2:243322/149‑1 (MQ=255)
                     ccGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGt                                                                                                                          <  1:2232871/149‑1 (MQ=255)
                      cGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTa                                                                                                                         >  2:243365/1‑149 (MQ=255)
                       ggATATGGCTTTCAAGCACTTCTGCCATCAGCCTATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTaa                                                                                                                        >  2:243341/1‑149 (MQ=255)
                       ggATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTaa                                                                                                                        >  2:243377/1‑149 (MQ=255)
                        gATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTaa                                                                                                                        <  1:243324/148‑1 (MQ=255)
                          tatGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCACTCTG‑CCCCGAATACGACGAACTCGAGTAAGGa                                                                                                                     <  2:243325/149‑1 (MQ=255)
                          tatGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGa                                                                                                                     >  1:243399/1‑149 (MQ=255)
                          tatGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGa                                                                                                                     <  2:243326/149‑1 (MQ=255)
                           atGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGAc                                                                                                                    >  1:243384/1‑149 (MQ=255)
                              gCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCtt                                                                                                                 >  2:243405/1‑149 (MQ=255)
                               cTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCttt                                                                                                                <  2:243327/149‑1 (MQ=255)
                                 ttCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCttttt                                                                                                              >  2:243393/1‑149 (MQ=255)
                                 ttCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCttttt                                                                                                              <  2:243329/149‑1 (MQ=255)
                                  tCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTttc                                                                                                             >  2:243380/1‑149 (MQ=255)
                                   cAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTttc                                                                                                             <  1:243330/148‑1 (MQ=255)
                                    aaGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTttctt                                                                                                           >  2:243374/1‑149 (MQ=255)
                                     aGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTttcttc                                                                                                          <  2:243332/149‑1 (MQ=255)
                                       cACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATCCGACGAACTCGAGTAAGGACCTTTTtcttctc                                                                                                        <  2:243335/149‑1 (MQ=255)
                                       cACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTtcttctc                                                                                                        <  2:243334/149‑1 (MQ=255)
                                         cTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGAc                                                                                                                    <  1:243336/135‑1 (MQ=255)
                                         cTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGAc                                                                                                                    >  2:243336/1‑135 (MQ=255)
                                         cTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTtcttctctt                                                                                                      >  1:243357/1‑149 (MQ=255)
                                          ttCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTtcttctcttc                                                                                                     >  1:243401/1‑149 (MQ=255)
                                               ccATCAGCCCATTAGCCGCGCCCCTCACGGAATCGATCTGTTGGAGTATGGCACTGCATTCTGCATCAACCTACAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATAAGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGa                                                                                                 <  1:243338/148‑1 (MQ=255)
                                                cATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGt                                                                                               >  2:243387/1‑149 (MQ=255)
                                                 aTCAGCCTATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTa                                                                                              <  1:243341/149‑1 (MQ=255)
                                                 aTCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTa                                                                                              <  2:243340/149‑1 (MQ=255)
                                                  tCAGCCCATTAGCCGCGCCCCGCACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTAc                                                                                             >  1:243392/1‑149 (MQ=255)
                                                   cAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACt                                                                                            <  2:243342/149‑1 (MQ=255)
                                                       ccATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTggg                                                                                         >  1:243394/1‑148 (MQ=255)
                                                       ccATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGc                                                                                        <  1:243344/149‑1 (MQ=255)
                                                         aTTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CACCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAt                                                                                      >  2:243446/1‑149 (MQ=255)
                                                          ttAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAtt                                                                                     <  1:243348/149‑1 (MQ=255)
                                                          ttAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAtt                                                                                     <  1:243349/149‑1 (MQ=255)
                                                          ttAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAtt                                                                                     <  2:243346/149‑1 (MQ=255)
                                                          ttAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCAtt                                                                                     <  1:243345/149‑1 (MQ=255)
                                                                  gcCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGaa                                                                                                                                 >  1:243388/1‑97 (MQ=255)
                                                                   ccccGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCtc                                                                            <  2:243350/149‑1 (MQ=255)
                                                                    cccGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCtca                                                                           <  1:243351/149‑1 (MQ=255)
                                                                       gAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCtcatca                                                                        <  2:243352/149‑1 (MQ=255)
                                                                          cGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCt                                                                     <  2:243355/149‑1 (MQ=255)
                                                                             cAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgca                                                                  <  2:243356/149‑1 (MQ=255)
                                                                              aGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgcat                                                                 <  2:243357/149‑1 (MQ=255)
                                                                              aGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgcat                                                                 >  1:243400/1‑149 (MQ=255)
                                                                               gCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTaa                                                                                                                        <  1:243358/93‑1 (MQ=255)
                                                                               gCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTGTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgcatg                                                                >  1:243420/1‑149 (MQ=255)
                                                                                 gATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgcatgca                                                              >  1:243395/1‑149 (MQ=255)
                                                                                 gATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCtgcatgca                                                              >  2:243402/1‑149 (MQ=255)
                                                                                   tCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGCCCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAat                                                            >  2:243458/1‑149 (MQ=255)
                                                                                   tCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCCCCTCATCATCTGCATGCAat                                                            >  2:243469/1‑149 (MQ=255)
                                                                                    cTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAata                                                           <  1:243359/149‑1 (MQ=255)
                                                                                       ttGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGCCGAACGCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCGCATCATCTGCATGCAAtataa                                                        >  2:243451/1‑148 (MQ=255)
                                                                                       ttGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGTAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATAc                                                        <  2:243361/149‑1 (MQ=255)
                                                                                         ggAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACta                                                      <  1:243362/149‑1 (MQ=255)
                                                                                           aGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACtata                                                    <  2:243363/149‑1 (MQ=255)
                                                                                             tATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAgg                                                  <  1:243364/149‑1 (MQ=255)
                                                                                             tATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAgg                                                  <  1:243365/149‑1 (MQ=255)
                                                                                                  cACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGt                                             <  2:243367/149‑1 (MQ=255)
                                                                                                      gCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTc                                         <  2:243368/149‑1 (MQ=255)
                                                                                                           cGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTatat                                    <  2:243370/149‑1 (MQ=255)
                                                                                                            ggCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATg                                   >  1:243417/1‑149 (MQ=255)
                                                                                                                tCACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCaa                               <  1:243371/149‑1 (MQ=255)
                                                                                                                 cACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGATTACGACGATCTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAAt                              <  1:243373/149‑1 (MQ=255)
                                                                                                                 cACCCTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAAt                              >  2:243450/1‑149 (MQ=255)
                                                                                                                 acaccTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCAGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAAt                              <  2:539669/146‑1 (MQ=255)
                                                                                                                     cTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCAt                          <  1:243374/149‑1 (MQ=255)
                                                                                                                     cTCCAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCAt                          >  2:243445/1‑149 (MQ=255)
                                                                                                                       ccAGCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATAc                        >  1:243375/1‑149 (MQ=255)
                                                                                                                          gCGACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATAcccc                     >  1:243440/1‑149 (MQ=255)
                                                                                                                            gACCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATa                         >  1:243424/1‑143 (MQ=255)
                                                                                                                             aCCGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCt                  <  1:243378/149‑1 (MQ=255)
                                                                                                                               cGTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCtat                >  2:243437/1‑149 (MQ=255)
                                                                                                                                gTTCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCtata               <  1:243379/149‑1 (MQ=255)
                                                                                                                                 ttCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATa                         >  1:243425/1‑138 (MQ=255)
                                                                                                                                  tCCAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAg              >  1:243439/1‑148 (MQ=255)
                                                                                                                                   ccAGAGCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGt             <  1:243380/148‑1 (MQ=255)
                                                                                                                                     agagCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGtata          <  1:243382/149‑1 (MQ=255)
                                                                                                                                     agagCATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGtata          >  1:243423/1‑149 (MQ=255)
                                                                                                                                         cATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAac      <  2:243384/149‑1 (MQ=255)
                                                                                                                                         cATCAATCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAac      >  1:243419/1‑149 (MQ=255)
                                                                                                                                             aaTCTG‑CCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTg  <  2:243385/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCGCCCCGAACGGCAGCGATCTGTTGGAGTATGGCACGGCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTG  >  U00096/379675‑379965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑