BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	U00096	2,173,363	Δ2 bp	intergenic (‑490/+918)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	2,173,361	0	C	.	99.2%	615.9 / ‑2.2	130	intergenic (‑488/+921)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (69/60); total (70/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*	U00096	2,173,362	0	C	.	99.2%	616.3 / ‑1.2	130	intergenic (‑489/+920)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (69/60); total (70/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 141 total aligned reads displayed.

CGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTA  >  U00096/2173213‑2173503

||

cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AACAATCAGGCGTGCCGATaccac                                                                                                                                                <  2:1418189/149‑1 (MQ=255)

 ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccacc                                                                                                                                               <  2:1418192/149‑1 (MQ=255)

   aaCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccacc                                                                                                                                               <  1:1418193/147‑1 (MQ=255)

    aCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                            >  2:1418249/1‑146 (MQ=255)

    aCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                            <  2:1418194/149‑4 (MQ=255)

     cAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccaccgccg                                                                                                                                           >  1:1418236/1‑145 (MQ=255)

       gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccaccgccgta                                                                                                                                         <  1:1418197/149‑7 (MQ=255)

        aTTACCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATaccaccgccgtat                                                                                                                                        >  1:1418243/1‑142 (MQ=255)

         tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATg                                                                                                                                     >  1:1418215/1‑149 (MQ=255)

           tCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCCGGCTTGCCGATACCA‑‑CCGCCGTATGTc                                                                                                                                   >  1:1418247/1‑149 (MQ=255)

           tCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTc                                                                                                                                   >  2:1418226/1‑149 (MQ=255)

             cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                                 <  2:1418198/149‑1 (MQ=255)

               cGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcag                                                                                                                               <  2:1418199/149‑1 (MQ=255)

               cGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcag                                                                                                                               >  2:1418234/1‑149 (MQ=255)

                 aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGTCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca                                                                                                                             <  2:1418200/149‑1 (MQ=255)

                 aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca                                                                                                                             >  2:1418306/1‑149 (MQ=255)

                  tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCC‑AACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                           >  2:1418242/1‑149 (MQ=255)

                          gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                                                                                                                     <  1:1418201/148‑1 (MQ=255)

                          gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCg                                                                                                                    >  1:1418316/1‑149 (MQ=255)

                              gCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                                >  1:1418269/1‑149 (MQ=255)

                                cATCGCCACGAAGACGCCGATGGTGGCAAGATCGCCAAACGGCCGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAACTCAGGCTTGCCGATACCA‑‑CCGCCGTATGACCCAGCAGCAACGCCGGATCaa                                                                                                              >  1:1418308/1‑149 (MQ=255)

                                 aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCACCGCCGGATCAAg                                                                                                             >  2:1418253/1‑149 (MQ=255)

                                  tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg                                                                                                            >  2:1418267/1‑149 (MQ=255)

                                    gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGcc                                                                                                          >  1:1418280/1‑149 (MQ=255)

                                     ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                         <  2:1418205/149‑1 (MQ=255)

                                     ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAG‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                         >  1:1418271/1‑149 (MQ=255)

                                      cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                                        <  2:1418206/149‑1 (MQ=255)

                                       aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                       >  1:1418298/1‑149 (MQ=255)

                                          aagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                    >  2:1418270/1‑149 (MQ=255)

                                            gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGa                                                                                                  <  2:1418209/149‑1 (MQ=255)

                                             aagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa                                                                                                 <  2:1418210/149‑1 (MQ=255)

                                                ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                              <  2:1418211/149‑1 (MQ=255)

                                                ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                              <  2:1418213/149‑1 (MQ=255)

                                                 cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt                                                                                                                 >  1:1418212/1‑129 (MQ=255)

                                                      gtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc                                                                                        <  2:1418214/149‑1 (MQ=255)

                                                        ggCAAGATCGCCAAACGGCAGCACCTGAATACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCCGCCTTGCCGATACCA‑‑CCGCCGTATGTAGCAGCAGCAACGCCGGATCAAGGCCAATCAGTAACTCCTGAccgc                                                                                      >  2:1418333/1‑149 (MQ=255)

                                                         gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                     >  1:1418281/1‑149 (MQ=255)

                                                            aGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGaa                                                                                  >  2:1418261/1‑149 (MQ=255)

                                                             gATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGa                                                                                   <  1:1418217/147‑1 (MQ=255)

                                                               tCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                               <  2:1418220/149‑1 (MQ=255)

                                                               tCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGACACCA‑‑CCGCCGTATGTCCCAGCAGCAACGACGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                               <  1:1418219/149‑1 (MQ=255)

                                                               tCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGGGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                               <  1:1418218/149‑1 (MQ=255)

                                                                cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                              <  2:1418221/149‑1 (MQ=255)

                                                                        ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑TAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                                      <  2:1418224/149‑1 (MQ=255)

                                                                        ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                                      >  1:1418266/1‑149 (MQ=255)

                                                                            gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                   >  2:1418300/1‑149 (MQ=255)

                                                                             caCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc                                                                 <  1:2213723/149‑1 (MQ=255)

                                                                             caCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCACGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc                                                                 <  1:1418226/149‑1 (MQ=255)

                                                                              aCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCCGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                >  2:1418278/1‑149 (MQ=255)

                                                                              aCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                <  2:1418227/149‑1 (MQ=255)

                                                                               ccTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCAGTAAACGACTa                                                               >  2:1418297/1‑149 (MQ=255)

                                                                                 tGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                             >  2:1418336/1‑149 (MQ=255)

                                                                                  gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa                                                            >  2:1418338/1‑149 (MQ=255)

                                                                                      aCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCt                                                                          >  1:1418229/1‑131 (MQ=255)

                                                                                       cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTg                                                       <  2:1418231/149‑1 (MQ=255)

                                                                                       cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACAGCCGAACTTCGCCTGTAAACGACTACGAGCCTg                                                       <  2:1418232/149‑1 (MQ=255)

                                                                                        ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc                                                      >  2:1418314/1‑149 (MQ=255)

                                                                                         cGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGa                                                                                          >  1:1418315/1‑112 (MQ=255)

                                                                                           gCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc                                                      <  1:1418234/146‑1 (MQ=255)

                                                                                            cacacaAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAg                                                  <  1:1418235/149‑1 (MQ=255)

                                                                                             acacaAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGc                                                 <  2:1418236/149‑1 (MQ=255)

                                                                                             acacaAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGc                                                 <  2:1418237/149‑1 (MQ=255)

                                                                                               acaATCAGCAATTAAAAAGGTGAGTGGGAT‑AAAAAACAGGCTTGCCGATACCACCCC‑CC‑TAAGTCCCAGCAGCAACGCCGCAACAAGCCCAAACAGGAACACCAGACCGCCCAACTTCCCCTCTAAACCACTACGACCCTGCTAAGc                                                 >  1:1418323/1‑147 (MQ=255)

                                                                                                 aaaCAGCAATTAAAATGGTGAGTGGTAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                                <  1:1418240/146‑1 (MQ=255)

                                                                                                 aaaCAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATg                                             <  1:1418241/149‑1 (MQ=255)

                                                                                                  aaCAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                            >  2:1418329/1‑149 (MQ=255)

                                                                                                   aCAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATggg                                           <  2:1418243/149‑1 (MQ=255)

                                                                                                   aCAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACAA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATggg                                           >  2:1418362/1‑149 (MQ=255)

                                                                                                     aGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                         <  2:1757161/149‑1 (MQ=255)

                                                                                                     aGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGATTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                         >  2:1418303/1‑149 (MQ=255)

                                                                                                      gCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCCGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt                                        >  2:1418326/1‑149 (MQ=255)

                                                                                                        aaTTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCACCGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                                      >  2:1418288/1‑149 (MQ=255)

                                                                                                        aaTTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                                      <  1:1418245/149‑1 (MQ=255)

                                                                                                        aaTTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGACGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATggg                                           >  1:1418341/1‑144 (MQ=255)

                                                                                                         aTTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGTCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaa                                     >  2:1418284/1‑149 (MQ=255)

                                                                                                          ttAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCACCGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                           >  1:1418340/1‑94 (MQ=255)

                                                                                                           tAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATg                                             >  1:1418283/1‑139 (MQ=255)

                                                                                                           tAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                   >  2:1418291/1‑149 (MQ=255)

                                                                                                            aaaaTGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGCAACTCCTGACCGCCGAACATCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGTCGGTAAAcc                                  <  2:1418247/149‑1 (MQ=255)

                                                                                                             aaaTGGTGAGTGGGAT‑AAAAATCAGGTTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                 >  1:1418307/1‑149 (MQ=255)

                                                                                                                tGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcc                              <  1:1418249/149‑1 (MQ=255)

                                                                                                                  gTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatccat                            >  1:1418289/1‑149 (MQ=255)

                                                                                                                    gAGTGGGAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                          <  2:1418250/149‑1 (MQ=255)

                                                                                                                        gggAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCTGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATggg                      <  1:1418253/149‑1 (MQ=255)

                                                                                                                         ggAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTATACCATCCATGATGGGt                     >  2:1418252/1‑149 (MQ=255)

                                                                                                                         ggAT‑AAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt                     >  1:1418287/1‑149 (MQ=255)

                                                                                                                               aaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                <  2:1418256/149‑1 (MQ=255)

                                                                                                                                aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc               <  2:1418257/149‑1 (MQ=255)

                                                                                                                                 aaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa              >  2:1418312/1‑149 (MQ=255)

                                                                                                                                  aTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc             >  1:1418321/1‑149 (MQ=255)

                                                                                                                                  aTCAGGCGTCCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa              <  1:1418258/148‑1 (MQ=255)

                                                                                                                                   tCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc            >  1:1418379/1‑149 (MQ=255)

                                                                                                                                    cAGGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc           <  1:1418259/149‑1 (MQ=255)

                                                                                                                                     aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCg          >  1:1418350/1‑149 (MQ=255)

                                                                                                                                         ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg       >  1:1418320/1‑148 (MQ=255)

                                                                                                                                         ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc      <  1:1418261/149‑1 (MQ=255)

                                                                                                                                         ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc      >  2:1418355/1‑149 (MQ=255)

                                                                                                                                          tGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc      >  1:1418364/1‑148 (MQ=255)

                                                                                                                                            ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt   <  1:1418262/149‑1 (MQ=255)

                                                                                                                                            ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt   >  2:1418378/1‑149 (MQ=255)

||

CGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAT‑AAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTA  >  U00096/2173213‑2173503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`