BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	3,153,487	Δ15 bp	intergenic (+64/‑62)	metC → / → yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	3153487	3153501	15	192 [0]	[0] 191	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 3153486	0 (0.000)	184 (0.990)	122/280	0.4	100%	intergenic (+63/‑77)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB
*	?	U00096	3153502 =	0 (0.000)	184 (0.990)	122/280	0.4	100%	intergenic (+79/‑61)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Only 100 of 191 total aligned reads displayed.

TGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACT  >  U00096/3153360‑3153650

gattcgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGa                                                                                                                                                <  2:2097544‑M2/7‑1 (MQ=255)

    cgcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCa                                                                                                                                            <  1:2097547‑M2/11‑1 (MQ=255)

     gcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAg                                                                                                                                           <  1:2097548‑M2/12‑1 (MQ=255)

     gcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAg                                                                                                                                           >  2:2097640‑M2/138‑149 (MQ=255)

     gcctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAg                                                                                                                                           >  1:2097583‑M2/138‑149 (MQ=255)

       ctgcatattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGa                                                                                                                                         >  1:2097711‑M2/136‑149 (MQ=255)

           atattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTAc                                                                                                                                     >  1:2097673‑M2/132‑149 (MQ=255)

            tattggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgagcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtcaCGGACGATTCAGTAGTACa                                                                                                                                    >  2:2097681‑M2/131‑149 (MQ=255)

                ggtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAg                                                                                                                                <  2:2097550‑M2/23‑1 (MQ=255)

                 gtctggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAgg                                                                                                                               >  2:2097674‑M2/126‑149 (MQ=255)

                     ggaagatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGc                                                                                                                              <  1:2097552‑M2/25‑1 (MQ=255)

                       aagatgtcgacgatctgattgcggatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATa                                                                                                                         <  2:2097553‑M2/30‑1 (MQ=255)

                         gatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATaaa                                                                                                                       >  1:2097695‑M2/118‑149 (MQ=255)

                         gatgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATaaa                                                                                                                       >  1:2097631‑M2/118‑149 (MQ=255)

                           tgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAgg                                                                                                                     <  1:2097558‑M2/34‑1 (MQ=255)

                           tgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAgg                                                                                                                     <  2:2097559‑M2/34‑1 (MQ=255)

                           tgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAgg                                                                                                                     >  2:2097626‑M2/116‑149 (MQ=255)

                           tgtcgacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAgg                                                                                                                     >  2:2097591‑M2/116‑149 (MQ=255)

                              cgacgatctgattgccgatctggacgccgggtttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCtt                                                                                                                  <  2:2097562‑M2/37‑1 (MQ=255)

                               gacgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTa                                                                                                                 <  2:2097563‑M2/38‑1 (MQ=255)

                                acgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                                >  2:2097662‑M2/111‑149 (MQ=255)

                                acgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaa                                                                                                                >  1:2097624‑M2/111‑149 (MQ=255)

                                acgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTa                                                                                                                 >  1:2097619‑M2/111‑148 (MQ=255)

                                 cgatctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCtt                                                                                                                  <  1:2097564‑M2/37‑1 (MQ=255)

                                   atctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACg                                                                                                             >  1:2097654‑M2/108‑149 (MQ=255)

                                   atctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACg                                                                                                             >  2:2097689‑M2/108‑149 (MQ=255)

                                    tctgattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaaa                                                                                                               >  1:2097642‑M2/107‑146 (MQ=255)

                                       gattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTaaa                                                                                                               <  1:2097566‑M2/40‑1 (MQ=255)

                                       gattgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGt                                                                                                         >  2:2097623‑M2/104‑149 (MQ=255)

                                        attgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGtt                                                                                                        <  1:2097569‑M2/47‑1 (MQ=255)

                                        attgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGtt                                                                                                        >  2:2097724‑M2/103‑149 (MQ=255)

                                          tgccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTcc                                                                                                      >  2:2097611‑M2/101‑149 (MQ=255)

                                            ccgatctggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCcac                                                                                                    >  1:2097706‑M2/99‑149 (MQ=255)

                                               atatggacgccggttttgcgcgaattgaataacatagccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAgg                                                                                                 <  2:2097572‑M2/54‑1 (MQ=255)

                                                   ggacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAgg                                                                                                 <  2:2097574‑M2/54‑1 (MQ=255)

                                                    gacgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCcaca                                                                                                   <  1:2097575‑M2/52‑1 (MQ=255)

                                                     acgccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTc                                                                                           >  1:2097656‑M2/90‑149 (MQ=255)

                                                       gccggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATGCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCa                                                                                         <  1:2097577‑M2/62‑1 (MQ=255)

                                                         cggttttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATg                                                                                       >  1:2097652‑M2/86‑149 (MQ=255)

                                                             tttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTg                                                                                   >  1:2097701‑M2/82‑149 (MQ=255)

                                                              ttgcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGt                                                                                  <  2:2097581‑M2/69‑1 (MQ=255)

                                                                gcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTa                                                                                >  2:2097639‑M2/79‑149 (MQ=255)

                                                                gcgcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTa                                                                                >  2:2097702‑M2/79‑149 (MQ=255)

                                                                  gcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTAtt                                                                              >  2:2119127‑M2/77‑149 (MQ=255)

                                                                  gcgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTAtt                                                                              >  1:2097715‑M2/77‑149 (MQ=255)

                                                                   cgaattgtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTc                                                                             <  2:2097583‑M2/74‑1 (MQ=255)

                                                                         gtataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGata                                                                       >  2:2097691‑M2/70‑149 (MQ=255)

                                                                           ataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGatat                                                                      >  1:2097684‑M2/68‑148 (MQ=255)

                                                                           ataacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc                                                                     >  2:2097696‑M2/68‑149 (MQ=255)

                                                                             aacattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGGTATTCACGATatcat                                                                   >  2:2097659‑M2/66‑149 (MQ=255)

                                                                              acattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc                                                                  <  1:2097587‑M2/85‑1 (MQ=255)

                                                                              acattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatca                                                                    >  1:2097704‑M2/65‑147 (MQ=255)

                                                                              acattgccacttttggacaattttgcagacactttattgtgaaaattcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTACACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc                                                                  <  2:2097586‑M2/85‑1 (MQ=255)

                                                                               cattgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCg                                                                 >  1:2097649‑M2/64‑149 (MQ=255)

                                                                                 ttgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCt                                                               >  2:2097634‑M2/62‑149 (MQ=255)

                                                                                  tgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTg                                                              >  2:2097753‑M2/61‑149 (MQ=255)

                                                                                  tgccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTg                                                              <  2:2097589‑M2/89‑1 (MQ=255)

                                                                                   gccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgc                                                             <  1:2097591‑M2/90‑1 (MQ=255)

                                                                                    ccacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat                                                                   >  1:2097742‑M2/59‑142 (MQ=255)

                                                                                     cacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcgc                                                           >  2:2097668‑M2/58‑149 (MQ=255)

                                                                                     cacttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTg                                                              >  1:2097719‑M2/58‑146 (MQ=255)

                                                                                      acttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGct                                                          >  2:2097636‑M2/57‑149 (MQ=255)

                                                                                        ttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctct                                                        <  1:2097595‑M2/95‑1 (MQ=255)

                                                                                        ttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTCCGctct                                                        >  2:2097732‑M2/55‑149 (MQ=255)

                                                                                        ttttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtcaCGGACGATTCAGGAGTACAATATGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctct                                                        <  1:2097593‑M2/95‑1 (MQ=255)

                                                                                         tttggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTg                                                       <  2:2097597‑M2/96‑1 (MQ=255)

                                                                                           tggacaattttgcagacattttattgtgaaaagtcttaaattattgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAGGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTgg                                                      >  1:2097665‑M2/52‑148 (MQ=255)

                                                                                            ggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCa                                                    >  1:2097687‑M2/51‑149 (MQ=255)

                                                                                            ggacaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCa                                                    >  1:2097667‑M2/51‑149 (MQ=255)

                                                                                              acaattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAac                                                  >  1:2097759‑M2/49‑149 (MQ=255)

                                                                                               caattttgcagacattttattttgaaaagtcttaaattgttgcggccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAAAGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                                 <  2:2097600‑M2/102‑1 (MQ=255)

                                                                                               caattttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca                                                 <  1:2097599‑M2/102‑1 (MQ=255)

                                                                                                  ttttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAGAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa                                              >  2:2097781‑M2/45‑149 (MQ=255)

                                                                                                    ttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACt                                            <  2:2097603‑M2/107‑1 (MQ=255)

                                                                                                    ttgcagacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACt                                            >  2:2097699‑M2/43‑149 (MQ=255)

                                                                                                     tgcagacattttattgtgaaaagtcttaaattgttgcggccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCCGCGCTCTGGCAACACGAc                                             <  1:2097604‑M2/106‑1 (MQ=255)

                                                                                                        agacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                                        >  2:2097670‑M2/39‑149 (MQ=255)

                                                                                                         gacattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGcc                                       <  1:2097607‑M2/112‑1 (MQ=255)

                                                                                                           cattttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc                                        >  1:2097728‑M2/36‑146 (MQ=255)

                                                                                                             ttttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                   >  2:2097735‑M2/34‑149 (MQ=255)

                                                                                                             ttttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc                                   <  2:2097608‑M2/116‑1 (MQ=255)

                                                                                                              tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt                                  >  1:2097740‑M2/33‑149 (MQ=255)

                                                                                                              tttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt                                  <  1:2097611‑M2/117‑1 (MQ=255)

                                                                                                              tttattgtgaaaagtcttaaattggggcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTGAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt                                  <  1:2097613‑M2/117‑1 (MQ=255)

                                                                                                               ttattgtgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTg                                 >  1:2097676‑M2/32‑149 (MQ=255)

                                                                                                                     tgaaaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTAGCAACACGACTTTGCCGCGCTGGCGGAt                           <  1:2097616‑M2/124‑1 (MQ=255)

                                                                                                                        aaagtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg                                >  1:2097721‑M2/23‑141 (MQ=255)

                                                                                                                          agtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa                      >  2:2097749‑M2/21‑149 (MQ=255)

                                                                                                                           gtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCat                     <  1:2097622‑M2/130‑1 (MQ=255)

                                                                                                                           gtcttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa                      >  1:2097682‑M2/20‑148 (MQ=255)

                                                                                                                             cttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCatat                   <  1:2097625‑M2/132‑1 (MQ=255)

                                                                                                                              ttaaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCatat                   >  1:2097725‑M2/17‑148 (MQ=255)

                                                                                                                                aaattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGt                >  2:2097713‑M2/15‑149 (MQ=255)

                                                                                                                                 aattgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt               >  2:2097693‑M2/14‑149 (MQ=255)

                                                                                                                                   ttgttgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAg             <  2:2097620‑M2/138‑1 (MQ=255)

                                                                                                                                       tgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgtt         <  2:2097617‑M2/142‑1 (MQ=255)

                                                                                                                                       tgcgtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgtt         >  2:2097743‑M2/8‑149 (MQ=255)

                                                                                                                                          gtccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgttgtt      <  1:2097627‑M2/145‑1 (MQ=255)

                                                                                                                                           tccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGttt     <  2:2097628‑M2/146‑1 (MQ=255)

                                                                                                                                            ccCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTa    <  2:2097630‑M2/147‑1 (MQ=255)

TGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTTACT  >  U00096/3153360‑3153650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`