BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	3,153,487	Δ15 bp	intergenic (+64/‑62)	metC → / → yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	3153487	3153501	15	245 [0]	[0] 243	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 3153486	0 (0.000)	234 (1.160)	149/280	0.1	100%	intergenic (+63/‑77)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB
*	?	U00096	3153502 =	0 (0.000)	234 (1.160)	149/280	0.1	100%	intergenic (+79/‑61)	metC/yghB	cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase/DedA family protein YghB

Only 100 of 245 total aligned reads displayed.

GGACCTTGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATT  >  U00096/3153338‑3153634

ggACCTTGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCC                                                                                                                                                      <  2:2272971‑M1/149‑1 (MQ=255)

  aCCTTGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcg                                                                                                                                                    >  2:2273078‑M1/1‑147 (MQ=255)

  aCCTTGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcg                                                                                                                                                    <  2:2272973‑M1/149‑3 (MQ=255)

    cTTGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcgg                                                                                                                                                   <  1:2272976‑M1/148‑4 (MQ=255)

     ttGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggac                                                                                                                                                 >  2:2273091‑M1/1‑144 (MQ=255)

           cGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattca                                                                                                                                           <  2:2272980‑M1/149‑12 (MQ=255)

            gCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcag                                                                                                                                          <  2:2272982‑M1/149‑13 (MQ=255)

              cTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcagga                                                                                                                                        >  2:2273045‑M1/1‑135 (MQ=255)

                   tatTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtaca                                                                                                                                   <  1:2272984‑M1/149‑20 (MQ=255)

                    atTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaa                                                                                                                                  <  1:2272986‑M1/149‑21 (MQ=255)

                    atTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaa                                                                                                                                  >  2:2273041‑M1/1‑129 (MQ=255)

                         tCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggc                                                                                                                             >  1:2273112‑M1/1‑124 (MQ=255)

                         tCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggc                                                                                                                             >  2:2273081‑M1/1‑124 (MQ=255)

                          cTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggca                                                                                                                            <  1:2272990‑M1/149‑27 (MQ=255)

                           tGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcag                                                                                                                           <  2:2272992‑M1/149‑28 (MQ=255)

                           tGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcag                                                                                                                           >  1:2273106‑M1/1‑122 (MQ=255)

                           tGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcag                                                                                                                           >  1:2273050‑M1/1‑122 (MQ=255)

                            ggAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggca                                                                                                                            <  1:2272994‑M1/147‑27 (MQ=255)

                               aGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataa                                                                                                                       <  1:2272997‑M1/149‑32 (MQ=255)

                                gATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGATAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaa                                                                                                                      <  2:2272999‑M1/149‑33 (MQ=255)

                                 aTGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcaga                                                                                                                          <  1:2273001‑M1/144‑29 (MQ=255)

                                      gacgaTCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggctta                                                                                                                <  1:2273007‑M1/149‑39 (MQ=255)

                                      gacgaTCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggctta                                                                                                                <  2:2273006‑M1/149‑39 (MQ=255)

                                       aagatcTGTTTGCCGATCTGGTCGCCGGTTTTGAGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGTAAAGTCTTAAATTGTTGCGTCCcggacgattcaggtgtacaataggcagataaaggcttaa                                                                                                               <  2:2273009‑M1/147‑40 (MQ=255)

                                         gaTCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaac                                                                                                             >  2:2273097‑M1/1‑108 (MQ=255)

                                              gATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgt                                                                                                        <  1:2273011‑M1/149‑47 (MQ=255)

                                              gATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTATGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgc                                                                                                           <  2:2273014‑M1/146‑44 (MQ=255)

                                                     gATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacag                                                                                                 >  1:2273123‑M1/1‑96 (MQ=255)

                                                      aTCTGGACGCCGGTGTTGCGCGAATTGTATAACATAGCCACTATTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAATTTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacagg                                                                                                >  1:2273092‑M1/1‑95 (MQ=255)

                                                        cTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaa                                                                                              <  2:2273018‑M1/149‑57 (MQ=255)

                                                        cTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaa                                                                                              <  1:2273019‑M1/149‑57 (MQ=255)

                                                         tGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaa                                                                                             >  1:2273067‑M1/1‑92 (MQ=255)

                                                         tGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaa                                                                                             >  2:2273088‑M1/1‑92 (MQ=255)

                                                           gACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagt                                                                                           <  2:2273024‑M1/149‑60 (MQ=255)

                                                           gACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccac                                                                                                   <  1:2273023‑M1/141‑52 (MQ=255)

                                                              gCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtcca                                                                                        <  1:2273028‑M1/149‑63 (MQ=255)

                                                              gCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtcca                                                                                        <  2:2273027‑M1/149‑63 (MQ=255)

                                                                cGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGCCATTTTAGTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatg                                                                                      >  2:2273165‑M1/1‑85 (MQ=255)

                                                                       gcgcGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgtta                                                                               <  2:2273030‑M1/149‑72 (MQ=255)

                                                                            aaTTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaa                                                                          >  2:2273127‑M1/1‑73 (MQ=255)

                                                                             aTTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaa                                                                          <  1:2273033‑M1/148‑77 (MQ=255)

                                                                              ttGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcg                                                                           >  2:2273117‑M1/1‑71 (MQ=255)

                                                                              ttGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattca                                                                           >  2:2273149‑M1/1‑71 (MQ=255)

                                                                               tGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagat                                                                       <  2:2273034‑M1/149‑80 (MQ=255)

                                                                                gTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagata                                                                      >  1:2273121‑M1/1‑69 (MQ=255)

                                                                                gTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagata                                                                      >  1:2273101‑M1/1‑69 (MQ=255)

                                                                                 tataACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatat                                                                     <  2:2273036‑M1/149‑82 (MQ=255)

                                                                                 tataACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttatacaagatat                                                                     >  2:2273154‑M1/1‑68 (MQ=255)

                                                                                   taACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatca                                                                   <  2:2273039‑M1/149‑84 (MQ=255)

                                                                                     aCATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatc                                                                 <  1:2273041‑M1/149‑86 (MQ=255)

                                                                                       aTTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgc                                                               <  1:2273045‑M1/149‑88 (MQ=255)

                                                                                          gCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgc                                                            >  2:2273114‑M1/1‑59 (MQ=255)

                                                                                           ccACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagatataggcttaaacgctgatccacaggaaagtccatggctgttattcaagatatcatcgctgcg                                                           >  2:2273143‑M1/1‑58 (MQ=255)

                                                                                                   ggACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggca                                                   >  2:2273047‑M1/1‑50 (MQ=255)

                                                                                                   ggACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggca                                                   >  1:2273171‑M1/1‑50 (MQ=255)

                                                                                                    gACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcttcgctgcgctctggca                                                   >  1:2273159‑M1/1‑49 (MQ=255)

                                                                                                    gACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggcggttattcaagatatcatcgctgcgctctggc                                                    >  1:2273182‑M1/1‑49 (MQ=255)

                                                                                                     aCAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaac                                                 <  2:2273050‑M1/149‑102 (MQ=255)

                                                                                                     aCAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaac                                                 <  2:2273052‑M1/149‑102 (MQ=255)

                                                                                                       aaTTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacac                                               <  1:2273054‑M1/149‑104 (MQ=255)

                                                                                                       aaTTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacac                                               >  1:2273203‑M1/1‑46 (MQ=255)

                                                                                                        aTTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacg                                              <  1:2273056‑M1/149‑105 (MQ=255)

                                                                                                        aTTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacac                                               >  1:2273220‑M1/1‑45 (MQ=255)

                                                                                                         ttttGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacga                                             >  1:2273128‑M1/1‑44 (MQ=255)

                                                                                                         ttttGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggc                                                                                    >  2:2273055‑M1/1‑44 (MQ=255)

                                                                                                         ttttGCAGACATTTTATTGTGAAAAGTCTTAAAATGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgatccacaggaaagtccatggctgttattcaagatatcatcgcagcgctctggcaacacga                                             >  2:2273188‑M1/1‑44 (MQ=255)

                                                                                                           ttGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagaccatgggggttattcaagatttcatcgctgcgctctggcaacacgact                                           >  1:2273185‑M1/1‑42 (MQ=255)

                                                                                                             gCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgacttt                                         >  2:2273176‑M1/1‑40 (MQ=255)

                                                                                                              cAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttg                                        >  2:2273207‑M1/1‑39 (MQ=255)

                                                                                                               aGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgacttt                                         >  1:2273133‑M1/1‑38 (MQ=255)

                                                                                                                 aCATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgcgctggcaacacgactttgccg                                     <  1:2273059‑M1/149‑114 (MQ=255)

                                                                                                                  cATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgcc                                      >  1:2273193‑M1/1‑35 (MQ=255)

                                                                                                                    ttttATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgc                                  >  2:2273132‑M1/1‑33 (MQ=255)

                                                                                                                    ttttATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgc                                  >  1:2273152‑M1/1‑33 (MQ=255)

                                                                                                                     tttATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgct                                 <  1:2273064‑M1/149‑118 (MQ=255)

                                                                                                                     tttATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgcgctggcaacacgactttgccgcgct                                 <  2:2273063‑M1/149‑118 (MQ=255)

                                                                                                                       tATTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctgg                               >  2:2273103‑M1/1‑30 (MQ=255)

                                                                                                                        aTTGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggc                              <  2:2273065‑M1/149‑121 (MQ=255)

                                                                                                                         ttGTGAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttg                                        >  1:2273145‑M1/1‑28 (MQ=255)

                                                                                                                          tgtgAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcgg                            <  2:2273068‑M1/149‑123 (MQ=255)

                                                                                                                            tgAAAAGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggat                          >  2:2273170‑M1/1‑25 (MQ=255)

                                                                                                                              aaaaGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcc                        <  1:2273070‑M1/149‑127 (MQ=255)

                                                                                                                               aaaGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacactgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcct                       <  1:2273075‑M1/149‑128 (MQ=255)

                                                                                                                                 aGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctca                     >  2:2273249‑M1/1‑20 (MQ=255)

                                                                                                                                 aGTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctca                     <  1:2273081‑M1/149‑130 (MQ=255)

                                                                                                                                  gTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcat                    <  2:2273085‑M1/149‑131 (MQ=255)

                                                                                                                                  gTCTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcat                    >  1:2273139‑M1/1‑19 (MQ=255)

                                                                                                                                    cTTAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatat                  >  1:2273148‑M1/1‑17 (MQ=255)

                                                                                                                                      tAAATTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattg                >  2:2273192‑M1/1‑15 (MQ=255)

                                                                                                                                       aaaTTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgt               >  2:2273213‑M1/1‑14 (MQ=255)

                                                                                                                                        aaTTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgacgttgccgcgctggcggatcctcatattgtt              >  2:2273230‑M1/1‑13 (MQ=255)

                                                                                                                                         aTTGTTGCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgtta             <  1:2273084‑M1/149‑138 (MQ=255)

                                                                                                                                           tgttgCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttagc           <  2:2273074‑M1/149‑140 (MQ=255)

                                                                                                                                           tgttgCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttagc           >  2:2273161‑M1/1‑10 (MQ=255)

                                                                                                                                           tgttgCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttag            <  1:2273078‑M1/148‑139 (MQ=255)

                                                                                                                                           tgttgCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttag            >  1:2273210‑M1/1‑10 (MQ=255)

                                                                                                                                            gttgCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttagc           >  1:2273164‑M1/1‑9 (MQ=255)

                                                                                                                                              tgCGTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttagcgtt        <  1:2273072‑M1/149‑143 (MQ=255)

                                                                                                                                                 gTCCcggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaacacgactttgccgcgctggcggatcctcatattgttagcgttgtt     <  1:2273088‑M1/149‑146 (MQ=255)

                                                                                                                                                    ccggacgattcaggagtacaataggcagataaaggcttaaacgctgttccacaggaaagtccatggctgttattcaagatatcatcgctgcgctctggcaccacgactttgccgcgctggcggatcctcatattgttagcgttgtttac  >  1:2273270‑M1/1‑1 (MQ=255)

GGACCTTGATTCGCCTGCATATTGGTCTGGAAGATGTCGACGATCTGATTGCCGATCTGGACGCCGGTTTTGCGCGAATTGTATAACATTGCCACTTTTGGACAATTTTGCAGACATTTTATTGTGAAAAGTCTTAAATTGTTGCGTCCGGGATCAAGGCGTCCCGGACGATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATT  >  U00096/3153338‑3153634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`