Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*U00096422,4290TG23.3% 264.8 / 22.7 129intergenic (+70/‑89)proY/malZputative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (14/85);  new base G (28/2);  total (42/87)
Fisher's exact test for biased strand distribution p-value = 9.95e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 134 total aligned reads displayed.

CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACT  >  U00096/422284‑422576
                                                                                                                                                 |                                                                                                                                                    
cgcgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcct                                                                                                                                                   <  2:290685/149‑1 (MQ=255)
cgcgTGGAGTGGGGGGCTGGTGATTGGCTGGATGTTTAAAAGCCGCCACGATCGTCAGCTGGCTGAAACCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcct                                                                                                                                                   <  2:290695/149‑1 (MQ=255)
  cgTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAACCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGgcctcc                                                                                                                                                 >  2:290805/1‑149 (MQ=255)
    tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCTGCTATTCCGAAAGTTAACCGCCCGCGtcgaccac                                                                                                                                               >  2:290781/1‑143 (MQ=255)
    tGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGgccgcct‑c                                                                                                                                              >  2:290792/1‑149 (MQ=255)
      gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑ccc                                                                                                                                            <  1:290687/149‑1 (MQ=255)
           ttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCaaa                                                                                                                                       <  2:290688/149‑1 (MQ=255)
            tgtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAat                                                                                                                                      <  1:290689/149‑1 (MQ=255)
              tgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAatat                                                                                                                                    <  2:2454297/149‑1 (MQ=255)
               gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATc                                                                                                                                   <  1:290690/149‑1 (MQ=255)
                cTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATcc                                                                                                                                  <  2:290693/149‑1 (MQ=255)
                 tGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGGGGCCG‑CCCCCAAATATCCt                                                                                                                                 >  2:290766/1‑149 (MQ=255)
                   ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTc                                                                                                                               <  2:290694/149‑1 (MQ=255)
                     gATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAg                                                                                                                             <  1:290696/149‑1 (MQ=255)
                       ttGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAgat                                                                                                                           <  2:290698/149‑1 (MQ=255)
                         ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAgatga                                                                                                                         <  1:290699/149‑1 (MQ=255)
                         ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAgatga                                                                                                                         <  2:290700/149‑1 (MQ=255)
                          gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAgatgat                                                                                                                        <  1:290701/149‑1 (MQ=255)
                              gATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCG‑CCCCCCAAAAACCGTCAGAAGAagagg                                                                                                                    >  2:290754/1‑148 (MQ=255)
                               aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGAtgatga                                                                                                                      <  1:290704/146‑1 (MQ=255)
                               aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                   <  1:290703/149‑1 (MQ=255)
                                   ttAAACTCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGTAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATcc                                                                                                               <  2:290705/149‑1 (MQ=255)
                                    tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGCGTGCT‑GCCCCCCAGATCCTTCAGATGGTGTGTGATcgg                                                                                                              >  2:290780/1‑147 (MQ=255)
                                       aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                           <  1:290707/149‑1 (MQ=255)
                                        cgccgcCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAt                                                                                                          <  2:290708/149‑1 (MQ=255)
                                         gccgccACGATCGTCAGCTGGCTGAAACCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCT‑CCCCCCAATATACGTAAGCTGAGGAGtgctgctgcctg                                                                                                         >  2:290832/1‑146 (MQ=255)
                                          ccgccACGATCTTCTTCTGGCTGTAAAGAAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAATTCCTGTTCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                        <  2:290710/149‑1 (MQ=255)
                                          ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTa                                                                                                        <  2:290709/149‑1 (MQ=255)
                                           cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                       <  2:290712/149‑1 (MQ=255)
                                               aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCG‑CGCCCCCATATCCGTCAGATGAGGGGTGCTGCTGCATTggggga                                                                                                   >  2:290824/1‑146 (MQ=255)
                                               aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTa                                                                                                   <  2:290714/149‑1 (MQ=255)
                                                cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAt                                                                                                  <  1:290715/149‑1 (MQ=255)
                                                 gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTATg                                                                                                 <  2:290716/149‑1 (MQ=255)
                                                    cGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                              <  2:290718/149‑1 (MQ=255)
                                                     gTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCaa                                                                                             <  2:694666/149‑1 (MQ=255)
                                                      tCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAg                                                                                            <  1:290719/149‑1 (MQ=255)
                                                       cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAgg                                                                                           <  1:290720/149‑1 (MQ=255)
                                                         gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTg                                                                                         <  1:290722/149‑1 (MQ=255)
                                                         gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCG‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATGAGGCTAGGGCAAgggg                                                                                         >  1:290791/1‑147 (MQ=255)
                                                         gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCT‑CCCCCCAATATCCTTCAGATGATGAGTGATGCTGCAGTAGGCTATGGGAAgggg                                                                                         >  1:290809/1‑147 (MQ=255)
                                                             ggctaaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCa                                                                                     <  1:290723/145‑1 (MQ=255)
                                                               tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCG‑CCCCCAAAGAGCCTGCACATGATGAGTGATCCTGCATTAGGGTAAGGGAAGGTGATc                                                                                      >  2:1743935/1‑146 (MQ=255)
                                                                gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGGGCCGGCGTCGTGCCGCCAAGTA‑AGTACAGATGATGAGTGATCCTGGAGTATGCTATGGCAAGGTggta                                                                                      >  2:290826/1‑142 (MQ=255)
                                                                gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCT‑CCCCCAAATATCCTTCAGATGATGAGGGATGCTGCATTAGGCTATGGGAAGGTGATc                                                                                      >  2:290796/1‑145 (MQ=255)
                                                                gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCACCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                  <  2:290724/149‑1 (MQ=255)
                                                                 aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTg                                                                                                                   <  1:290725/115‑1 (MQ=255)
                                                                    aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCG‑CCCCCAAAAATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                              >  2:290810/1‑149 (MQ=255)
                                                                      cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCGCCG‑CCCCCGAAGATCCAGCAGAGGATGAGTGATGCTGGAGTAGGCTATGGCAAGGTGGTCAGATTTtcat                                                                            >  2:290778/1‑149 (MQ=255)
                                                                      cAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCG‑CCCCCAAAAATCCTTCAGATGATGAGTGATCCGGCAGTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                            >  2:290827/1‑149 (MQ=255)
                                                                        gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCG‑CGCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCGAGGGGAAGGGGATAAGATTTtgatca                                                                          >  2:290790/1‑149 (MQ=255)
                                                                         tactCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcac                                                                         <  1:290727/146‑1 (MQ=255)
                                                                           ctacTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                       <  2:1327593/146‑1 (MQ=255)
                                                                             ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                     <  1:290729/149‑1 (MQ=255)
                                                                              cTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgggg                                                                    <  2:290730/149‑1 (MQ=255)
                                                                               ttGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATGAGATTTTCATCACAGGGGa                                                                   >  2:290833/1‑149 (MQ=255)
                                                                                tGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                  <  2:290731/149‑1 (MQ=255)
                                                                                tGCGTTCTGTAAGCCGGGTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGaa                                                                  <  2:290732/149‑1 (MQ=255)
                                                                                 gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCT‑CCCCCCCAAAGCCTGAAGATGATGAGTGATCCTGGAGTAGGCGATGGGAAGGTGAGCGGATTTTCATGACAGTGGaag                                                                 >  2:290844/1‑148 (MQ=255)
                                                                                    ttCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCGGCCT‑CCCCCAAAAAACAATCAGATGATTACTGGTCCTGCATTAGGGGATGGGAAGGTGGTCAGATTTTCTTAACAGGGGAttttt                                                              >  2:290838/1‑147 (MQ=255)
                                                                                     tCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                <  1:290733/146‑1 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                          <  2:290736/149‑1 (MQ=255)
                                                                                        gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCAGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatgatg                                                          <  2:290735/149‑1 (MQ=255)
                                                                                          aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                        <  2:290737/149‑1 (MQ=255)
                                                                                             ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                     <  2:290740/149‑1 (MQ=255)
                                                                                             ccGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaaa                                                     <  2:290739/149‑1 (MQ=255)
                                                                                                gTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGc                                                  <  2:290741/149‑1 (MQ=255)
                                                                                                 ttCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCGT‑CCCCCAAAGAACCGTCCGAGGATGAGTGAGCCTGGATTAGGCTATGGGAAGGTGAGCAGATTTTCATCACAGGGGAATGATGATGTTATATGCa                                                 >  2:290859/1‑149 (MQ=255)
                                                                                                  tCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                <  1:290743/149‑1 (MQ=255)
                                                                                                   cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                               <  2:290745/149‑1 (MQ=255)
                                                                                                   cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATg                                               <  1:290746/149‑1 (MQ=255)
                                                                                                     ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGc                                             <  2:290747/149‑1 (MQ=255)
                                                                                                     ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCG‑CCCCCAAATATGCGTCAGATGATGAGGGTTCCTGGAGTAGGGGATGGCAAGGTGGTCAGATTTTGATGACAGGGGGATTATGATGTTTTATGAATGGc                                             >  2:290854/1‑149 (MQ=255)
                                                                                                      gaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                            <  2:290748/149‑1 (MQ=255)
                                                                                                       aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                           <  2:290750/149‑1 (MQ=255)
                                                                                                       aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCAc                                           <  1:290749/149‑1 (MQ=255)
                                                                                                          aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGGCTCCT‑CCCCCCAAAATCATTCGGCGGATGAGTGATCATGGATTAGGCGATTGGAAGGTGATATGATTTTCATGAAAGGGGTATTATTATGTTTAATGAATTGCACATg                                        >  2:290799/1‑149 (MQ=255)
                                                                                                           aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                       <  2:290751/149‑1 (MQ=255)
                                                                                                           aaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGc                                       <  2:290752/149‑1 (MQ=255)
                                                                                                                        cTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                          <  2:290756/149‑1 (MQ=255)
                                                                                                                         tATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAttt                         <  2:290757/149‑1 (MQ=255)
                                                                                                                         tATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                            <  1:290759/146‑1 (MQ=255)
                                                                                                                          aTTCCGAAAGTTATCCGCCGCCGGCCTCCT‑CCCCCAAAAATCATTAAGATGATGAGTGATACTGCATTAGGCTATGGCAAGGTGATAAGATTTTAATAAAAGGGGAATTATGATGTTAAATTAATGGAACCTGCCGGTGCCCCCaaatg                        >  2:290816/1‑145 (MQ=255)
                                                                                                                             ccGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTatga                                                            <  1:290760/110‑1 (MQ=255)
                                                                                                                               gAAAGTTATCCGCCCCCGGCCGCCG‑CCCCCAAATAACCTTCAGATGATGAGTGATGCTGCATTAGGGTATGGCAAGGTGATAAGATTTTGATCACAGGGGAATTATTATGTTTAATGCATGGCACCTGCCGGGGGCCCCCTATGTTaaa                   >  2:290864/1‑149 (MQ=255)
                                                                                                                                 aaGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                 <  2:290761/149‑1 (MQ=255)
                                                                                                                                    ttATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag              <  2:290763/149‑1 (MQ=255)
                                                                                                                                    ttATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGGGATCAGAGTTTCATCACAGGGGAATTATGATGTGAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaag              <  2:290762/149‑1 (MQ=255)
                                                                                                                                     tATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagc             <  1:290764/149‑1 (MQ=255)
                                                                                                                                     ggtgcGCCCCCGTCCTCCG‑CCCCCACATATCCGTGAGATGCTGAGTGATCCTGCGTTAGGCTAGGGCAAGGTGATCAGCTTTTAGTCACAGGGGAAGTATGATTTTAAATGCATGGCCCCTGCCGGTGCCCCCATTTGTTAAAcaaagc             >  1:290891/5‑149 (MQ=255)
                                                                                                                                      aTCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagca            <  1:290766/149‑1 (MQ=255)
                                                                                                                                       tCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa           <  2:290767/149‑1 (MQ=255)
                                                                                                                                         cGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag         <  1:290768/149‑1 (MQ=255)
                                                                                                                                           cccccGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa        <  1:290769/148‑1 (MQ=255)
                                                                                                                                            ccccGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaa           >  2:290815/1‑144 (MQ=255)
                                                                                                                                            ccccGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAAGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc      >  2:290841/1‑149 (MQ=255)
                                                                                                                                              ccGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa    >  1:290830/1‑149 (MQ=255)
                                                                                                                                              ccGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa    >  2:290846/1‑149 (MQ=255)
                                                                                                                                              ccGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa    >  2:290894/1‑149 (MQ=255)
                                                                                                                                                gTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  >  2:290800/1‑149 (MQ=255)
                                                                                                                                                gTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt  <  2:290771/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCT‑CCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACT  >  U00096/422284‑422576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑