Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	U00096	2,947,465	0	T	.	21.7%	165.5 / 42.8	61	intergenic (+2/‑32)	metZ/metW	tRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (7/40);  new base . (5/8);  total (12/49)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCG  >  U00096/2947317‑2947560                                                                                                                                                     |                                                                                                   ccAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                                    <  1:2114000/149‑1 (MQ=255) ccAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                                    <  2:2114001/149‑1 (MQ=255)  ctaaTCCGGGAGCGGCTTATAACTAAGGGTGCATGAAATCGAGAGGGTAGTATTGTGCGCCTCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtc                                                                                                   <  2:2114101/147‑2 (MQ=14)  cAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAtt                                                                                                   >  2:2114139/1‑149 (MQ=255)    aaTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaa                                                                                                 <  2:2114004/149‑1 (MQ=255)     aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                <  1:2114007/149‑1 (MQ=255)     aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa                                                                                                <  1:2114008/149‑1 (MQ=255)     aTCCGTGAGCGGGGTAAAATAAAGGTTGCATGAAAAAGCGAGCGTAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                                                <  2:2114006/149‑1 (MQ=21)      tCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaaa                                                                                               <  2:2114011/149‑1 (MQ=255)        cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAtt                                                                                             <  1:2114012/149‑1 (MQ=255)         gTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                                            <  2:2114013/149‑1 (MQ=255)         gTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAtttg                                                                                            <  2:2114014/149‑2 (MQ=255)          tGATCGGTGTAAAACAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTGCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                                           <  1:2114015/149‑1 (MQ=255)          tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                                           <  1:2114016/149‑1 (MQ=255)          tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtga                                                                                          >  2:2114040/1‑149 (MQ=255)           gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                          <  2:2114018/149‑1 (MQ=255)           gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                          <  1:2114017/149‑1 (MQ=255)           gATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgat                                                                                         >  1:2114020/1‑149 (MQ=255)            aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat                                                                                         <  2:2114019/149‑1 (MQ=255)            aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgat‑g                                                                                       <  2:2114020/149‑1 (MQ=255)             tCGGGGTAAAAAAAAGGTTTCATGAAAAAGCGAGCTGAGTATAGTCCGCATCCACGGACGCTGTGTGGAGCAGCCTGGTATCTCGTCGGGCTCATAACCCGAATGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat‑g                                                                                       <  2:2114022/149‑1 (MQ=21)             gcGGTGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCTGGGTGGAGCAGCCTGGTAGCTCGTCTGGCTCATAACCCTAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat‑g                                                                                       <  2:2114025/148‑1 (MQ=255)                  gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCTGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑a                                                                                <  1:2114024/149‑1 (MQ=255)                  gTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtg                                                                                           <  1:2114026/141‑1 (MQ=255)                   tAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aa                                                                               <  1:2114028/149‑1 (MQ=255)                   tAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aa                                                                               <  2:2114031/149‑1 (MQ=255)                   tAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aa                                                                               <  1:2114029/149‑1 (MQ=255)                   tAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aa                                                                               <  1:2114030/149‑1 (MQ=255)                   tAAAAAAAAGGTGGCATGAAAACGCGCGCGTAGTATAGTGCGCATCCACGTACGCTGTGTGGAGCAGCATGTTAGCTCGTCGGGCTCATAAACCGAAGGTCGGCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aa                                                                               <  1:2265912/149‑1 (MQ=21)                    aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aaa                                                                              <  1:2114033/149‑1 (MQ=255)                    aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑aaa                                                                              <  1:2114034/149‑1 (MQ=255)                     aaaacaaGGTTGCATGAAAACGCGAGCGTAGTATAGTGCGCATCCCCGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑AAGT‑aa                                                                               <  2:2114036/146‑1 (MQ=17)                     aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑a                                                                                >  2:2114032/1‑146 (MQ=255)                     aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑a                                                                                <  1:2114032/146‑1 (MQ=255)                     aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAg                                                                             <  1:2114037/149‑1 (MQ=255)                     aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAg                                                                             <  1:2114039/149‑1 (MQ=255)                      aatcaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCAGGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑AAGT‑aaaagc                                                                           <  2:2114042/145‑4 (MQ=17)                      aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAA‑TTGAT‑G‑AAGG‑aaaag                                                                            >  1:2114036/1‑146 (MQ=255)                      aaaaaaGGTTGCATGAAAACGCCAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑AAGT‑aaa                                                                              <  1:2114040/146‑1 (MQ=255)                          aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGt                                                                         <  1:2114041/148‑1 (MQ=255)                          aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAA‑GTAGGA‑AAAGCAGTa                                                                        >  1:2114087/1‑149 (MQ=17)                           aGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                  >  1:2114043/1‑125 (MQ=255)                            ggTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGAAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTGGTGG‑AGGT‑AAAGCAGTACg                                                                      >  1:2114117/1‑149 (MQ=18)                             gTTGCATGAAAACGCTAGCGTTGTATTGTGCGCTTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑AAGTAAAAGCAGTACgg                                                                     <  2:2114087/149‑1 (MQ=11)                             gTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACgg                                                                     <  1:2114046/149‑1 (MQ=255)                             gTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                                                                                >  1:2114045/1‑125 (MQ=255)                             gTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑AAGTAAAAGCAGTACgg                                                                     >  2:2114076/1‑149 (MQ=255)                              ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGt                                                                    <  1:2114047/149‑1 (MQ=255)                              ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCGCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGt                                                                    <  2:2114048/149‑1 (MQ=255)                               tGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTg                                                                   <  2:2114052/149‑1 (MQ=255)                               tGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTg                                                                   <  1:2114051/149‑1 (MQ=255)                               tGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAAGCGGGGTGGAGCAGCCTGGTAGCTCGTGGGGCTCATAACCCGAAGGTCGTCGGCTCAAATCCGGCCCCAGCAACCAATTAAAATTTGAT‑T‑AAGT‑AAAGCAGTACGGt                                                                    <  1:2114049/148‑1 (MQ=21)                                   tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGAcgc                                                               <  2:2114053/149‑1 (MQ=255)                                   tGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGAc                                                                 <  1:2114054/147‑1 (MQ=255)                                   tGAAAACGCCAGCGGAGTATAGTCGGCTTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑AAGTAAAAGCAGTACGGTGAcgc                                                               <  2:2114102/149‑1 (MQ=11)                                     aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                                                                                    >  1:2114094/1‑113 (MQ=255)                                      aaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGAT‑G‑aa                                                                                    <  2:2114045/125‑1 (MQ=255)                                       aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGACGCgggg                                                           <  2:2114056/149‑1 (MQ=255)                                        aCGCCAGCGGAGTATAGCCCGCCTCCACGGACTCGGGGTGTAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGGCGGCGGTTCATATCCGGCCCCCGGAACCAA‑TAAAATTTGAT‑T‑TACTAAAAGCAGTACGGTGACGCGGGGt                                                          <  2:2114117/149‑1 (MQ=11)                                            gAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCTGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                                            <  1:2114058/114‑1 (MQ=255)                                              gCGGAGTATAGTGCGCATCCACGGACGCGGTGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGACGCGGGGTGGagca                                                    <  2:2114064/149‑1 (MQ=255)                                                 gAGTATAGTGATCATCCTCTGAATCGTTTTGGAGCAGCCTTGTCGCTCGGCGGTATCATAACCCTAAGGTCGGCGGGTCTAATCCTTCCCCCGCAACCAAATAAATTTTGTT‑G‑AATT‑TACGCATTCCTGGGACGCGGGGTGGAGCAGcc                                                 <  2:2114097/149‑1 (MQ=9)                                                        gTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                  >  1:2114146/1‑96 (MQ=255)                                                         tGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTa                                                                                                  >  1:2114147/1‑95 (MQ=255)                                                                    ggACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTAATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACGAATTAAATTTTGTT‑G‑AAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                              <  2:2114068/149‑1 (MQ=255)                                                                                                                                                   aaTTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg  >  2:2114132/1‑98 (MQ=255)                                                                                                                                                     |                                                                                                   CCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAT‑G‑AAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCG  >  U00096/2947317‑2947560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑